Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,560	+C	intergenic (‑129/‑732)	ppiC ← / → rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,558	1	.	C	100.0%	533.5 / NA	146	intergenic (‑127/‑734)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (64/82); total (64/82)

Only 100 of 154 total aligned reads displayed.

TAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑T‑GC‑TCGCAGGT‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957470‑3957645

tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGT‑c‑c                                                                                       <  1:4646966/93‑1 (MQ=255)

 aGCATGATCCGCAGATGCTAGCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGT‑c‑cc                                                                                      <  1:894075/93‑2 (MQ=255)

   cATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGT‑c‑cctc                                                                                    >  2:4558835/1‑90 (MQ=255)

    aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGT‑c‑cctca                                                                                   <  1:3594481/93‑5 (MQ=255)

        tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAggg                                                                                >  1:6015253/1‑93 (MQ=255)

        tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAggg                                                                                >  2:4325677/1‑93 (MQ=255)

        tCCGCAAATGCAAACGTAATGGCCAGCCAGGGAGTGGCTGGTAAATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAggg                                                                                <  1:1513959/93‑1 (MQ=255)

         ccGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAgggg                                                                               <  2:5923120/93‑1 (MQ=255)

          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGga                                                                              <  2:1189619/93‑1 (MQ=255)

          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGga                                                                              <  2:3388717/93‑1 (MQ=255)

          cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGga                                                                              <  1:5241577/93‑1 (MQ=255)

               aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGaaaa                                                                         >  1:308986/1‑93 (MQ=255)

                tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAAc                                                                        >  1:2605310/1‑93 (MQ=255)

                 gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACt                                                                       >  1:2938992/1‑93 (MQ=255)

                 gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCGTCGCAGGTCC‑CTCAAGGGGAGAAAACt                                                                       >  2:1293094/1‑93 (MQ=255)

                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAg                                                                    <  2:1925912/93‑1 (MQ=255)

                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAg                                                                    <  1:1018440/93‑1 (MQ=255)

                    aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAg                                                                    >  1:1900277/1‑93 (MQ=255)

                       gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGc                                                                 <  2:1798259/93‑1 (MQ=255)

                       gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGc                                                                 >  1:3249213/1‑93 (MQ=255)

                       gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGT‑CTCTCAAGGGGAGAAAACTTAGGGc                                                                 >  1:3443540/1‑93 (MQ=255)

                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGcc                                                                <  1:1056185/93‑1 (MQ=255)

                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGcc                                                                >  2:1670348/1‑93 (MQ=255)

                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGcc                                                                <  1:770468/93‑1 (MQ=255)

                        tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGcc                                                                <  2:5958304/93‑1 (MQ=255)

                         aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCct                                                               <  2:6071234/93‑1 (MQ=255)

                          aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCctc                                                              <  2:4959993/93‑1 (MQ=255)

                            tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  1:2866087/93‑1 (MQ=255)

                             gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  1:3707158/93‑1 (MQ=255)

                               cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                         >  2:4989816/1‑93 (MQ=255)

                                agtcagGGAGTGGCTGACACATATATGGCAAAACCCCTGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATggg                                                        <  2:2739448/93‑1 (MQ=255)

                                  tgagGGAGTGGCTGGTACATATATGGCAAAAGCCGGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      <  2:1298734/91‑1 (MQ=255)

                                  tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      >  1:4757245/1‑93 (MQ=255)

                                  tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      >  1:3289571/1‑93 (MQ=255)

                                  tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                      >  2:1566262/1‑93 (MQ=255)

                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGGGAAAACTTAGGGCCCCCATggggtcc                                                    >  2:1508563/1‑89 (MQ=255)

                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    <  2:2358848/93‑1 (MQ=255)

                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    <  1:619340/93‑1 (MQ=255)

                                    agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  2:1508564/1‑93 (MQ=255)

                                     gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:4555456/1‑93 (MQ=255)

                                     gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:1727812/1‑93 (MQ=255)

                                      ggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                                  <  2:3183266/93‑1 (MQ=255)

                                       gAGTGGCTGGTACATATATGGCAAAAGCCGGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 <  2:4444119/93‑1 (MQ=255)

                                       gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 <  2:585246/93‑1 (MQ=255)

                                          tGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                              >  1:4814745/1‑93 (MQ=255)

                                            gCTGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                                            <  1:2671362/93‑1 (MQ=255)

                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          <  1:5584220/93‑1 (MQ=255)

                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          >  1:4211893/1‑93 (MQ=255)

                                              tGGTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          >  1:1533937/1‑93 (MQ=255)

                                               ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTTTATGGGCTACAAAGTCATTAt                                         <  1:4971884/93‑1 (MQ=255)

                                               ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         >  1:5911035/1‑93 (MQ=255)

                                               ggTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  2:2162068/93‑1 (MQ=255)

                                               ggTACATATATGGCAAAAGCCCGAAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  1:2561476/93‑1 (MQ=255)

                                                gTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                        >  1:3562583/1‑93 (MQ=255)

                                                gTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                        <  2:3238432/93‑1 (MQ=255)

                                                gTACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGAGCCTCTATGGGCTACAAAGTCATTATc                                        >  2:235248/1‑93 (MQ=255)

                                                 tACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                                       >  2:4773745/1‑93 (MQ=255)

                                                 tACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                                       >  2:2836440/1‑93 (MQ=255)

                                                 tACATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                                       <  1:4456508/93‑1 (MQ=255)

                                                  aCATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      >  1:4966817/1‑93 (MQ=255)

                                                   cATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     <  1:3971308/93‑1 (MQ=255)

                                                   cATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     >  2:2286091/1‑93 (MQ=255)

                                                   cATATATGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     >  1:5961297/1‑93 (MQ=255)

                                                    atatatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    >  1:3787/1‑93 (MQ=255)

                                                    atatatGGCAAAAGCCCGGAAAATCC‑GGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    >  2:802404/1‑93 (MQ=255)

                                                     tatatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   <  1:5811190/93‑1 (MQ=255)

                                                     tatatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  2:260567/1‑93 (MQ=255)

                                                     tatatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  2:4207929/1‑93 (MQ=255)

                                                     aatatGGCAAAAGCCCGGAAANTCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   <  2:1772790/92‑1 (MQ=255)

                                                      atagGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                                  <  2:158199/93‑1 (MQ=255)

                                                       tatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                                 <  2:5639335/93‑1 (MQ=255)

                                                       tatGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                                 <  1:3159988/93‑1 (MQ=255)

                                                         tGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                               <  1:5699089/93‑1 (MQ=255)

                                                         tGGCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                               <  2:2594229/93‑1 (MQ=255)

                                                          ggCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                              >  2:674351/1‑93 (MQ=255)

                                                           gCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                             <  1:870907/93‑1 (MQ=255)

                                                           gCAAAAGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                             <  1:3366842/93‑1 (MQ=255)

                                                              aaaGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                          <  2:417131/93‑1 (MQ=255)

                                                               aaGCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttt                         <  2:3318719/93‑1 (MQ=255)

                                                                aGCCCGGAAAATCC‑GGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                        >  2:5262722/1‑93 (MQ=255)

                                                                 gCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       <  2:1168370/93‑1 (MQ=255)

                                                                 gCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       >  2:3086145/1‑93 (MQ=255)

                                                                 gCCCGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat                       >  2:2094205/1‑93 (MQ=255)

                                                                  cccGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      <  1:1553052/93‑1 (MQ=255)

                                                                  cccGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      >  1:469397/1‑93 (MQ=255)

                                                                  cccGGAAAATCC‑GGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      >  1:2499675/1‑93 (MQ=255)

                                                                   ccGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  2:3619124/1‑93 (MQ=255)

                                                                   ccGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  1:560076/1‑93 (MQ=255)

                                                                   ccGGAAAATCCGG‑GCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     <  2:3791210/93‑1 (MQ=255)

                                                                                  cttCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCg       >  2:3060731/3‑93 (MQ=255)

                                                                                   ttCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      >  1:811851/2‑93 (MQ=255)

                                                                                   ttCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      <  1:2432112/92‑1 (MQ=255)

                                                                                    tCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     <  2:1035643/93‑1 (MQ=255)

                                                                                    tCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     <  2:2204374/93‑1 (MQ=255)

                                                                                    tCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     <  2:3057886/93‑1 (MQ=255)

                                                                                    tCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     >  1:1733906/1‑93 (MQ=255)

                                                                                     cGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata    <  1:1168048/93‑1 (MQ=255)

                                                                                     cGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata    <  1:636644/93‑1 (MQ=255)

                                                                                       cAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  <  1:5362107/93‑1 (MQ=255)

                                                                                       cAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  >  2:835629/1‑93 (MQ=255)

TAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCC‑T‑GC‑TCGCAGGT‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957470‑3957645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCAGGT‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  NC_000913/3957478‑3957653

TCCGCAAATGCAAACGTAATGGCCAGCCAGGGAGTGGCTGGTAAATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGG                                                                                       <  SRR4302532.1565089/94‑1 (MQ=34)

  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAG                                                                                     <  SRR4302532.5422281/94‑1 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCCAGGGGAGAAA                                                                                  >  SRR4302532.175324/1‑94 (MQ=60)

      GATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAA                                                                                 >  SRR4302532.319700/1‑94 (MQ=60)

       ATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAAC                                                                                >  SRR4302532.2692445/1‑94 (MQ=60)

       ATGCTAACGTAATGGTCAGTCGGGGAGTGGCTGGTACATATAGGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAAC                                                                                <  SRR4302532.3712800/94‑1 (MQ=57)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACT                                                                               >  SRR4302532.3036606/1‑94 (MQ=60)

           TAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAG                                                                            >  SRR4302532.1963294/1‑94 (MQ=60)

            AACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGG                                                                           <  SRR4302532.1052998/94‑1 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTCGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGG                                                                          <  SRR4302532.3307454/94‑1 (MQ=60)

              CGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGC                                                                         >  SRR4302532.3357094/1‑94 (MQ=60)

              CGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGT‑CTCTCAAGGGGAGAAAACTTAGGGC                                                                         >  SRR4302532.3558911/1‑94 (MQ=60)

                TAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCT                                                                       <  SRR4302532.1091780/94‑1 (MQ=60)

                TAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCT                                                                       <  SRR4302532.797056/94‑1 (MQ=60)

                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                   <  SRR4302532.36448/94‑1 (MQ=60)

                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                   <  SRR4302532.2961952/94‑1 (MQ=60)

                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  <  SRR4302532.3831612/94‑1 (MQ=60)

                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                                 <  SRR4302532.1167266/94‑1 (MQ=60)

                        AGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGC                                                               <  SRR4302532.539546/94‑1 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              >  SRR4302532.4920009/1‑94 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              >  SRR4302532.3399002/1‑94 (MQ=60)

                         GTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCT                                                              <  SRR4302532.4626818/94‑1 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                            >  SRR4302532.4189080/1‑94 (MQ=60)

                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           <  SRR4302532.640927/94‑1 (MQ=60)

                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                        <  SRR4302532.5670115/94‑1 (MQ=60)

                                atgggaTGGTACATATATGGCAAAAGCCGGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                                       <  SRR4302532.754152/88‑1 (MQ=60)

                                AGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                                       <  SRR4302532.5054761/94‑1 (MQ=60)

                                AGTGGCTGGTACATATAAGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGT                                                       <  SRR4302532.2063092/94‑1 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      <  SRR4302532.3408990/94‑1 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      >  SRR4302532.4980533/1‑94 (MQ=60)

                                    GCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATT                                                   <  SRR4302532.2761018/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  >  SRR4302532.4353268/1‑94 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  >  SRR4302532.4907254/1‑94 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  <  SRR4302532.938826/94‑1 (MQ=60)

                                     CTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTA                                                  >  SRR4302532.1585766/1‑94 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                                 <  SRR4302532.5778083/94‑1 (MQ=60)

                                      TGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAT                                                 >  SRR4302532.6115746/1‑94 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTTTATGGGCTACAAAGTCATTATC                                                <  SRR4302532.5142330/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                >  SRR4302532.264363/1‑94 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                <  SRR4302532.6164139/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                <  SRR4302532.2757391/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                >  SRR4302532.3681776/1‑94 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGAAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                <  SRR4302532.2646935/94‑1 (MQ=60)

                                         TACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                              >  SRR4302532.5137121/1‑94 (MQ=60)

                                         TACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGG                                              <  SRR4302532.4608406/94‑1 (MQ=60)

                                          ACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                             >  SRR4302532.6167455/1‑94 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302532.3944/1‑94 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            <  SRR4302532.4105087/94‑1 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302532.6012801/1‑94 (MQ=60)

                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                          <  SRR4302532.710480/94‑1 (MQ=60)

                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                          <  SRR4302532.4012325/94‑1 (MQ=60)

                                             TATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAT                                          <  SRR4302532.6013074/94‑1 (MQ=60)

                                               TATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGG                                        <  SRR4302532.3264911/94‑1 (MQ=60)

                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                                      <  SRR4302532.5897878/94‑1 (MQ=60)

                                                 TGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTT                                      <  SRR4302532.6300765/94‑1 (MQ=60)

                                                   GCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                    <  SRR4302532.900730/94‑1 (MQ=60)

                                                   GCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                    <  SRR4302532.3479377/94‑1 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302532.2855111/1‑94 (MQ=60)

                                                      AAAGCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                                 >  SRR4302532.3725681/1‑94 (MQ=60)

                                                         GCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                              >  SRR4302532.485748/1‑94 (MQ=60)

                                                         GCCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATT                              >  SRR4302532.2583028/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             <  SRR4302532.1605565/94‑1 (MQ=60)

                                                          CCCGGAAAATCCGGGCTTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             >  SRR4302532.579600/1‑94 (MQ=60)

                                                            cggaaaatccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTC                           <  SRR4302532.3057609/79‑1 (MQ=60)

                                                             ggaaaatccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                          >  SRR4302532.1583036/15‑94 (MQ=60)

                                                                aaatccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       >  SRR4302532.5299622/12‑94 (MQ=60)

                                                                aaatccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       <  SRR4302532.5586381/83‑1 (MQ=60)

                                                                 aatccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTT                      >  SRR4302532.5830244/11‑94 (MQ=60)

                                                                  atccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                     >  SRR4302532.2379702/10‑94 (MQ=60)

                                                                  atccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                     >  SRR4302532.4636875/10‑94 (MQ=60)

                                                                   tccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAT                    <  SRR4302532.6179839/86‑1 (MQ=60)

                                                                   tccgggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAT                    <  SRR4302532.5368813/86‑1 (MQ=60)

                                                                      gggctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302532.5038049/89‑1 (MQ=60)

                                                                         ctTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              >  SRR4302532.839555/3‑94 (MQ=60)

                                                                          TTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGAT             >  SRR4302532.1792266/1‑94 (MQ=60)

                                                                          TTCGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGAT             <  SRR4302532.2512962/94‑1 (MQ=60)

                                                                            CGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302532.1207067/94‑1 (MQ=60)

                                                                            CGCAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302532.658837/94‑1 (MQ=60)

                                                                              CAGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         <  SRR4302532.5548006/94‑1 (MQ=60)

                                                                               AGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        >  SRR4302532.638147/1‑94 (MQ=60)

                                                                               AGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        >  SRR4302532.2830980/1‑94 (MQ=60)

                                                                               AGGTCC‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        <  SRR4302532.5446978/94‑1 (MQ=60)

                                                                                  C‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACCAAGTCATTATCGGGGATGGTTATTTTATTGTGAATTTATGAGCGATATGGCATTG    >  SRR4302532.4444286/1‑94 (MQ=60)

                                                                                  C‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    <  SRR4302532.3997324/94‑1 (MQ=60)

                                                                                  C‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    <  SRR4302532.3199797/94‑1 (MQ=60)

                                                                                  C‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    <  SRR4302532.1163097/94‑1 (MQ=60)

                                                                                    C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302532.1369577/1‑94 (MQ=60)

                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  <  SRR4302532.5997325/94‑1 (MQ=60)

                                                                                      CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  <  SRR4302532.4215448/94‑1 (MQ=60)

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CTCGCAGGT‑C‑CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  NC_000913/3957478‑3957653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`