Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,972,637 2 bp→GC coding (430‑431/546 nt) wecD → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,6370CG100.0% 634.7 / NA 179R144A (CGT→GCT) wecDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (77/102);  total (77/102)
*NC_0009133,972,6380GC100.0% 646.7 / NA 179R144A (CGT→GCT) wecDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (77/102);  total (77/102)

Only 100 of 182 total aligned reads displayed.

CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972550‑3972724
                                                                                       ||                                                                                      
cTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTcgc                                                                                    <  1:273145/93‑1 (MQ=255)
    aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTa                                                                                >  2:3107223/1‑93 (MQ=255)
    aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTa                                                                                >  2:2538613/1‑93 (MQ=255)
     tGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaa                                                                               >  1:1463961/1‑93 (MQ=255)
      gCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaa                                                                              <  1:4911144/93‑1 (MQ=255)
       cGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaaa                                                                             <  2:854531/93‑1 (MQ=255)
       cGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaaaa                                                                             <  1:2238298/93‑1 (MQ=255)
        gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaac                                                                            <  2:302303/93‑1 (MQ=255)
          cAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaa                                                                          <  1:5173075/93‑1 (MQ=255)
          cAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaa                                                                          >  2:3402974/1‑93 (MQ=255)
           aGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaac                                                                         <  1:687880/93‑1 (MQ=255)
           aGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaac                                                                         <  1:3889085/93‑1 (MQ=255)
             aTGCGCGAAGTGTCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACtt                                                                       <  2:2156126/93‑1 (MQ=255)
              tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACCACttt                                                                      >  1:2319317/1‑93 (MQ=255)
               gcgcGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTg                                                                     <  1:3032238/93‑1 (MQ=255)
                 gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCg                                                                   >  1:1944594/1‑93 (MQ=255)
                  cgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCgg                                                                  >  2:1068042/1‑93 (MQ=255)
                   gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCggg                                                                 <  1:4266644/93‑1 (MQ=255)
                   gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCggg                                                                 <  1:4136682/93‑1 (MQ=255)
                    aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt                                                                <  1:696166/93‑1 (MQ=255)
                    aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt                                                                >  1:6096575/1‑93 (MQ=255)
                     aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg                                                               <  1:3447093/93‑1 (MQ=255)
                      ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTgg                                                              <  2:2341673/93‑1 (MQ=255)
                      ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTgg                                                              >  2:4457875/1‑93 (MQ=255)
                       tGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTTGc                                                             <  1:4390700/93‑1 (MQ=255)
                         gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCgc                                                           >  2:1970804/1‑92 (MQ=255)
                            tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:5952463/1‑93 (MQ=255)
                            tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:2554068/1‑93 (MQ=255)
                             gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCa                                                       >  1:4184856/1‑93 (MQ=255)
                             gctggctgGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCa                                                       >  2:4501630/1‑93 (MQ=255)
                              ctggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaa                                                      >  2:1206223/1‑93 (MQ=255)
                                ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    >  1:2439139/1‑93 (MQ=255)
                                ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    <  2:4069621/93‑1 (MQ=255)
                                 gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  2:3745551/93‑1 (MQ=255)
                                 gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   <  1:1285925/93‑1 (MQ=255)
                                  ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  2:5133841/93‑1 (MQ=255)
                                  ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  2:605052/93‑1 (MQ=255)
                                  ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  >  2:3907587/1‑93 (MQ=255)
                                   tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACGCCAATggg                                                 >  1:491762/1‑93 (MQ=255)
                                   tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 >  1:766828/1‑93 (MQ=255)
                                    ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:1978896/93‑1 (MQ=255)
                                    ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  1:5271655/93‑1 (MQ=255)
                                    ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  1:2375789/93‑1 (MQ=255)
                                    ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGGGGAGACCCAAATGGGc                                                >  1:448909/1‑93 (MQ=255)
                                     gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGCCCCAAATGGGCa                                               >  2:1892562/1‑93 (MQ=255)
                                        gcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAaca                                            >  1:5079734/1‑93 (MQ=255)
                                         cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           >  1:5226289/1‑93 (MQ=255)
                                          ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          >  1:3583455/1‑93 (MQ=255)
                                          ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          <  2:1633051/93‑1 (MQ=255)
                                           gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg                                         <  2:412714/93‑1 (MQ=255)
                                            tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        <  2:383961/93‑1 (MQ=255)
                                                ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg                                    <  1:4550056/93‑1 (MQ=255)
                                                  tGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt                                  >  1:2792757/1‑93 (MQ=255)
                                                     tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa                               >  2:5904412/1‑93 (MQ=255)
                                                      gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              <  2:276249/93‑1 (MQ=255)
                                                       agctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  1:5212911/93‑1 (MQ=255)
                                                       agctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAc                             <  2:661517/93‑1 (MQ=255)
                                                        tctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTTAAACAACTTTGCGGGTGGCTTTCCTAATGGGCAATACCGCCGCGCTTaaatg                            >  1:5462581/2‑91 (MQ=255)
                                                         ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           <  2:3963990/93‑1 (MQ=255)
                                                         ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           <  2:5919158/93‑1 (MQ=255)
                                                          tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          <  2:4464186/93‑1 (MQ=255)
                                                          tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          >  1:3580367/1‑93 (MQ=255)
                                                          tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          <  1:1153915/93‑1 (MQ=255)
                                                           gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:1488124/1‑93 (MQ=255)
                                                           gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:5766017/1‑93 (MQ=255)
                                                           gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:1252710/1‑93 (MQ=255)
                                                            aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        >  2:5752257/1‑93 (MQ=255)
                                                            aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        >  2:2890343/1‑93 (MQ=255)
                                                             tGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       >  2:209150/1‑93 (MQ=255)
                                                             tGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       >  2:4559440/1‑93 (MQ=255)
                                                               cAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata                     >  1:1192870/1‑93 (MQ=255)
                                                               cAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacata                     <  2:3397439/93‑1 (MQ=255)
                                                                aaaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacatac                    >  1:135498/1‑93 (MQ=255)
                                                                  aCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  <  1:5029372/93‑1 (MQ=255)
                                                                       gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:3232708/1‑93 (MQ=255)
                                                                       gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             <  2:5032346/93‑1 (MQ=255)
                                                                       gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             <  1:5669839/93‑1 (MQ=255)
                                                                       gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  2:4830476/1‑93 (MQ=255)
                                                                       gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             <  1:4506921/93‑1 (MQ=255)
                                                                        cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  1:3915871/93‑1 (MQ=255)
                                                                        cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:1021951/93‑1 (MQ=255)
                                                                        cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  1:4287773/93‑1 (MQ=255)
                                                                         tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           >  1:1892229/1‑93 (MQ=255)
                                                                         tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           >  1:906379/1‑93 (MQ=255)
                                                                          aaaCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          <  1:1784242/93‑1 (MQ=255)
                                                                          aaaCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          <  2:5785862/93‑1 (MQ=255)
                                                                          aaaCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          >  1:3868759/1‑93 (MQ=255)
                                                                           aaCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCg         >  2:3501350/1‑93 (MQ=255)
                                                                            aCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGa        <  2:1573585/93‑1 (MQ=255)
                                                                             cTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       >  1:4662674/1‑93 (MQ=255)
                                                                             cTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       <  1:2329011/93‑1 (MQ=255)
                                                                              tGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      >  2:426982/1‑93 (MQ=255)
                                                                              tGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  2:4178128/93‑1 (MQ=255)
                                                                              tGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      >  1:4743424/1‑93 (MQ=255)
                                                                               gggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     <  2:2693309/93‑1 (MQ=255)
                                                                                ggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGt    <  2:4636536/93‑1 (MQ=255)
                                                                                ggCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGt    >  1:2886773/1‑93 (MQ=255)
                                                                                 gCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  2:5325688/93‑1 (MQ=255)
                                                                                 gCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  2:5645952/93‑1 (MQ=255)
                                                                                  cGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAg  <  1:4046601/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972550‑3972724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  NC_000913/3972549‑3972726
                                                                                        |                                                                                         
ACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGC                                                                                      <  SRR4302532.5731632/94‑1 (MQ=60)
 CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCG                                                                                     <  SRR4302532.806603/94‑1 (MQ=60)
 CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCG                                                                                     <  SRR4302532.6254258/94‑1 (MQ=60)
 CTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCG                                                                                     <  SRR4302532.282607/94‑1 (MQ=60)
  TCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGG                                                                                    <  SRR4302532.2105374/94‑1 (MQ=60)
     ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAA                                                                                 >  SRR4302532.4955426/1‑94 (MQ=60)
     ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAA                                                                                 >  SRR4302532.1513286/1‑94 (MQ=60)
      TGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAA                                                                                >  SRR4302532.4000175/1‑94 (MQ=60)
       GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAA                                                                               <  SRR4302532.246895/94‑1 (MQ=60)
       GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAA                                                                               <  SRR4302532.5079894/94‑1 (MQ=60)
        CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAAC                                                                              <  SRR4302532.2312576/94‑1 (MQ=60)
        CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAAC                                                                              <  SRR4302532.280604/94‑1 (MQ=60)
        CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAAC                                                                              <  SRR4302532.5150948/94‑1 (MQ=60)
         GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACA                                                                             >  SRR4302532.4057249/1‑94 (MQ=60)
          ACAGATGCGCGAATTGGCCTGCTGGCTGGACGGGGTGCAGGGGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAAAAA                                                                            >  SRR4302532.4775817/1‑94 (MQ=60)
           CAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAAC                                                                           <  SRR4302532.5351237/94‑1 (MQ=60)
            AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACT                                                                          <  SRR4302532.4020310/94‑1 (MQ=60)
            AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACT                                                                          <  SRR4302532.711470/94‑1 (MQ=60)
            AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACT                                                                          >  SRR4302532.5235067/1‑94 (MQ=60)
              ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACCACTTT                                                                        >  SRR4302532.2396507/1‑94 (MQ=60)
              ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTT                                                                        <  SRR4302532.4505809/94‑1 (MQ=60)
               TGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTG                                                                       <  SRR4302532.4698328/94‑1 (MQ=60)
                GCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGC                                                                      <  SRR4302532.3132297/94‑1 (MQ=60)
                 CGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCG                                                                     >  SRR4302532.2009254/1‑94 (MQ=60)
                  GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGG                                                                    <  SRR4302532.3039353/94‑1 (MQ=60)
                    GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  >  SRR4302532.6307013/1‑94 (MQ=60)
                    GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  <  SRR4302532.4410262/94‑1 (MQ=60)
                    GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  <  SRR4302532.172513/94‑1 (MQ=60)
                    GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  <  SRR4302532.2901348/94‑1 (MQ=60)
                    GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  <  SRR4302532.4275446/94‑1 (MQ=60)
                    GAATGGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  <  SRR4302532.4275447/94‑1 (MQ=60)
                     AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 <  SRR4302532.720007/94‑1 (MQ=60)
                     AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 <  SRR4302532.3279999/94‑1 (MQ=60)
                      ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGG                                                                <  SRR4302532.3562559/94‑1 (MQ=60)
                       TTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGC                                                               >  SRR4302532.1491461/1‑94 (MQ=60)
                        TGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTTGCG                                                              <  SRR4302532.4539979/94‑1 (MQ=60)
                            CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCC                                                          >  SRR4302532.2311596/1‑94 (MQ=60)
                            CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCC                                                          >  SRR4302532.6158419/1‑94 (MQ=60)
                            CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCC                                                          >  SRR4302532.2639253/1‑94 (MQ=60)
                             TGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCA                                                         >  SRR4302532.4325242/1‑94 (MQ=60)
                                TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                      >  SRR4302532.2520241/1‑94 (MQ=60)
                                TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                      <  SRR4302532.139524/94‑1 (MQ=60)
                                 GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                     <  SRR4302532.4834484/94‑1 (MQ=60)
                                  GCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGG                                                    <  SRR4302532.1328939/94‑1 (MQ=60)
                                   CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACGCCAATGGG                                                   >  SRR4302532.508945/1‑94 (MQ=60)
                                   CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGG                                                   >  SRR4302532.793284/1‑94 (MQ=60)
                                    TGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGGGGAGACCCAAATGGGC                                                  >  SRR4302532.464501/1‑94 (MQ=60)
                                     GGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                 <  SRR4302532.2454978/94‑1 (MQ=60)
                                     GGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                 <  SRR4302532.719461/94‑1 (MQ=60)
                                     GGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                 <  SRR4302532.5453435/94‑1 (MQ=60)
                                        CGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACA                                              <  SRR4302532.3695595/94‑1 (MQ=60)
                                        CGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACA                                              >  SRR4302532.5254381/1‑94 (MQ=60)
                                         GCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAC                                             >  SRR4302532.5311449/1‑94 (MQ=60)
                                         GCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAC                                             >  SRR4302532.5406546/1‑94 (MQ=60)
                                          CGTTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAACCAACTTTGCGGGTGGCCACCCAACTGGGCAACACC                                            <  SRR4302532.4429605/94‑1 (MQ=60)
                                          CGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACC                                            >  SRR4302532.3703326/1‑94 (MQ=60)
                                                 GGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGC                                     <  SRR4302532.4705327/94‑1 (MQ=60)
                                                  GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                    >  SRR4302532.2886389/1‑94 (MQ=60)
                                                       GAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAC                               >  SRR4302532.2348873/1‑94 (MQ=60)
                                                        ATCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTTAAACAACTTTGCGGGTGGCTTTCCTAATGGGCAATACCGCCGCGCTTAAATG                              >  SRR4302532.5651967/1‑94 (MQ=55)
                                                        AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACATCGCCGCGCTTAAACG                              <  SRR4302532.393237/94‑1 (MQ=60)
                                                        AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                              <  SRR4302532.5392685/94‑1 (MQ=60)
                                                        AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                              <  SRR4302532.5392682/94‑1 (MQ=60)
                                                        AGCTGATGCAAACCGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                              <  SRR4302532.5392681/94‑1 (MQ=60)
                                                          CTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAT                            >  SRR4302532.4043586/1‑94 (MQ=60)
                                                          CTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAT                            >  SRR4302532.3700139/1‑94 (MQ=60)
                                                           TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           >  SRR4302532.5966655/1‑94 (MQ=60)
                                                           TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           >  SRR4302532.1538337/1‑94 (MQ=60)
                                                           TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           >  SRR4302532.1294636/1‑94 (MQ=60)
                                                           TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           <  SRR4302532.1192414/94‑1 (MQ=60)
                                                            GATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATAC                          <  SRR4302532.5505913/94‑1 (MQ=60)
                                                            GATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATAC                          <  SRR4302532.6284674/94‑1 (MQ=60)
                                                             ATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                         <  SRR4302532.2001825/94‑1 (MQ=60)
                                                              TGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                        <  SRR4302532.4582862/94‑1 (MQ=60)
                                                              TGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                        >  SRR4302532.1326067/1‑94 (MQ=60)
                                                              TGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTGGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                        <  SRR4302532.2526552/94‑1 (MQ=60)
                                                               GCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATA                       >  SRR4302532.1232755/1‑94 (MQ=60)
                                                                CAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC                      >  SRR4302532.140069/1‑94 (MQ=60)
                                                                   ACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAA                   <  SRR4302532.5201878/94‑1 (MQ=60)
                                                                    CGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAG                  <  SRR4302532.979385/94‑1 (MQ=60)
                                                                      GCGCTAAACTGGGCGGATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                <  SRR4302532.603823/94‑1 (MQ=60)
                                                                       CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               >  SRR4302532.3339998/1‑94 (MQ=60)
                                                                       CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               >  SRR4302532.4399783/1‑94 (MQ=60)
                                                                       CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               <  SRR4302532.5880049/94‑1 (MQ=60)
                                                                        GCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT              <  SRR4302532.4660808/94‑1 (MQ=60)
                                                                        GCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT              <  SRR4302532.5867553/94‑1 (MQ=60)
                                                                         CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             >  SRR4302532.1954969/1‑94 (MQ=60)
                                                                         CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             <  SRR4302532.1864184/94‑1 (MQ=60)
                                                                         CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             >  SRR4302532.1276520/1‑94 (MQ=60)
                                                                         CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             <  SRR4302532.4047833/94‑1 (MQ=60)
                                                                         CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             >  SRR4302532.937325/1‑94 (MQ=60)
                                                                         CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             <  SRR4302532.4432393/94‑1 (MQ=60)
                                                                          TAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGC            >  SRR4302532.3999315/1‑94 (MQ=60)
                                                                           AAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCG           <  SRR4302532.1843904/94‑1 (MQ=60)
                                                                             ACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAA         >  SRR4302532.4821656/1‑94 (MQ=60)
                                                                              CTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT        >  SRR4302532.4905439/1‑94 (MQ=60)
                                                                              CTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT        <  SRR4302532.2406582/94‑1 (MQ=60)
                                                                                GGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT      <  SRR4302532.3488439/94‑1 (MQ=60)
                                                                                GGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT      >  SRR4302532.2983226/1‑94 (MQ=60)
                                                                                 GGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA     <  SRR4302532.752171/94‑1 (MQ=60)
                                                                                 GGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA     >  SRR4302532.6067525/1‑94 (MQ=60)
                                                                                  GCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG    <  SRR4302532.578889/94‑1 (MQ=60)
                                                                                  GCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG    <  SRR4302532.844834/94‑1 (MQ=60)
                                                                                   CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   >  SRR4302532.2764240/1‑94 (MQ=60)
                                                                                   CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   >  SRR4302532.3619485/1‑94 (MQ=60)
                                                                                   CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   <  SRR4302532.4182826/94‑1 (MQ=60)
                                                                                   CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   >  SRR4302532.1227470/1‑94 (MQ=60)
                                                                                    GTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  SRR4302532.2001217/1‑94 (MQ=60)
                                                                                        |                                                                                         
ACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  NC_000913/3972549‑3972726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑