Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,918 2 bp→CG coding (348‑349/1236 nt) aslB → putative arylsulfatase regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9180GC100.0% 827.0 / NA 229W116C (TGG→TGC) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (88/141);  total (88/141)
*NC_0009133,980,9190CG100.0% 837.8 / NA 234P117A (CCG→GCG) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (88/146);  total (88/146)

Only 100 of 238 total aligned reads displayed.

CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005
                                                                                       ||                                                                                      
cgtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGtt                                                                                    >  1:1076209/1‑93 (MQ=255)
cgtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGtt                                                                                    >  2:2686859/1‑93 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  1:3996376/1‑93 (MQ=255)
  tAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc                                                                                  >  1:988757/1‑93 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  2:1245531/93‑1 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  2:648382/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                >  1:6132681/1‑93 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  2:1999203/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  1:5588394/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  2:5010476/93‑1 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGCTAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              >  1:2877327/1‑93 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              >  2:101382/1‑93 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              <  1:1444450/93‑1 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              <  1:4065643/93‑1 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              <  2:5380016/93‑1 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             <  1:1506992/93‑1 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             <  1:5062202/93‑1 (MQ=255)
        tACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCgg                                                                            >  1:3476666/1‑93 (MQ=255)
        tACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCgg                                                                            <  1:3906299/93‑1 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  2:5158290/93‑1 (MQ=255)
            ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                        >  2:4181878/1‑93 (MQ=255)
              cGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatc                                                                      >  2:6021633/1‑93 (MQ=255)
              cGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatc                                                                      >  2:181418/1‑93 (MQ=255)
               gAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                     <  2:5623360/93‑1 (MQ=255)
               gAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                     <  1:1596065/93‑1 (MQ=255)
                aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                    <  2:1581877/93‑1 (MQ=255)
                aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                    <  2:2392027/93‑1 (MQ=255)
                 aaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                   <  1:2047987/93‑1 (MQ=255)
                  atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAttt                                                                  <  1:890955/93‑1 (MQ=255)
                    atGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc                                                                <  1:2698477/93‑1 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  1:4893691/93‑1 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               >  2:2510794/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  2:4311290/93‑1 (MQ=255)
                        tGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgt                                                            <  1:3229431/93‑1 (MQ=255)
                         gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt                                                           <  1:5517296/93‑1 (MQ=255)
                         gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGAATCATTTTCttgtt                                                           >  2:2958561/1‑93 (MQ=255)
                          cTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgttg                                                          >  2:3549562/1‑93 (MQ=255)
                            ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                        <  2:2122796/93‑1 (MQ=255)
                             gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                       <  1:1569443/93‑1 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     <  2:1775234/93‑1 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     >  2:4794845/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    <  2:5322240/93‑1 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    <  2:3146049/93‑1 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    >  1:2110101/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    <  2:3936319/93‑1 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    >  2:4008227/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    <  1:3664097/93‑1 (MQ=255)
                                  aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                  <  1:5340867/93‑1 (MQ=255)
                                  aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                  <  2:2996052/93‑1 (MQ=255)
                                  aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                  >  1:3323063/1‑93 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc                                                 <  2:861707/93‑1 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTCTCTTGTTGGTTTATCGc                                                 <  2:3564905/93‑1 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                >  1:5637976/1‑93 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                <  1:788192/93‑1 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  1:1866875/1‑93 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  1:2994604/1‑93 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              <  1:470407/93‑1 (MQ=255)
                                       aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                             <  2:545292/93‑1 (MQ=255)
                                       aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                             <  1:4340284/93‑1 (MQ=255)
                                       aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                             <  1:5418633/93‑1 (MQ=255)
                                          tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATgg                                          <  2:5279880/93‑1 (MQ=255)
                                          tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATgg                                          <  1:2606845/93‑1 (MQ=255)
                                           aaCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGTTTTATCGCTGGATGGc                                         >  2:2047917/1‑93 (MQ=255)
                                           aaCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGc                                         <  2:52144/93‑1 (MQ=255)
                                           aaCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGc                                         >  2:6071440/1‑93 (MQ=255)
                                            aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGGGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc                                        >  1:4015587/1‑93 (MQ=255)
                                             cAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGccc                                       <  2:1097182/93‑1 (MQ=255)
                                               gCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGc                                     >  1:5836337/1‑93 (MQ=255)
                                               gCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGc                                     >  1:4740863/1‑93 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   <  2:284663/93‑1 (MQ=255)
                                                 ttCCAAACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   <  2:4679734/93‑1 (MQ=255)
                                                    cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgag                                >  1:1046180/1‑93 (MQ=255)
                                                     aGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgaga                               <  2:47307/93‑1 (MQ=255)
                                                     aGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgaga                               <  1:3169084/93‑1 (MQ=255)
                                                      gACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAt                              >  1:3129836/1‑93 (MQ=255)
                                                       aCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATc                             <  2:2767454/93‑1 (MQ=255)
                                                          aaCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCCGAGATCcac                          >  1:6034943/1‑93 (MQ=255)
                                                            cGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaa                        <  2:4484117/93‑1 (MQ=255)
                                                             ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                       >  2:202141/1‑93 (MQ=255)
                                                             ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                       <  1:3553325/93‑1 (MQ=255)
                                                                gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaa                    <  2:2184858/93‑1 (MQ=255)
                                                                gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCCGCGATCCAccatcaa                    >  1:1406237/1‑93 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  >  1:2967054/1‑93 (MQ=255)
                                                                   ctgctgGATGACGAATGGTGCGCGTTTCTCTCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGTTGGCCCGCCTGAGATCCAAAATCAatat                 >  2:4921394/1‑93 (MQ=255)
                                                                        ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  1:2917054/93‑1 (MQ=255)
                                                                        ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            >  1:5900741/1‑93 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         >  2:192845/1‑93 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         <  1:873379/93‑1 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         <  2:3287838/93‑1 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         <  2:145603/93‑1 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         <  2:2732319/93‑1 (MQ=255)
                                                                            gACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACt        <  1:427648/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  2:757654/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  1:832740/93‑1 (MQ=255)
                                                                              cGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaa      <  1:2360939/93‑1 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    <  2:3830677/93‑1 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    >  1:4768371/1‑93 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    <  2:5667783/93‑1 (MQ=255)
                                                                                  tggtgcGCGTTTCTTGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt  <  1:4540793/93‑1 (MQ=255)
                                                                                  tggtgcGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt  <  1:1160601/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980830‑3981007
                                                                                        |                                                                                         
CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTT                                                                                      <  SRR4302532.385827/94‑1 (MQ=60)
CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTT                                                                                      >  SRR4302532.1112324/1‑94 (MQ=60)
 CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     >  SRR4302532.961855/1‑94 (MQ=60)
 CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     >  SRR4302532.4131001/1‑94 (MQ=60)
 CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     <  SRR4302532.1537325/94‑1 (MQ=60)
 CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     <  SRR4302532.1537324/94‑1 (MQ=60)
  GTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTC                                                                                    >  SRR4302532.1022533/1‑94 (MQ=60)
  GTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTC                                                                                    <  SRR4302532.4319650/94‑1 (MQ=60)
    AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  >  SRR4302532.6344258/1‑94 (MQ=60)
     GCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCG                                                                                 <  SRR4302532.425008/94‑1 (MQ=60)
     GCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCG                                                                                 <  SRR4302532.5782393/94‑1 (MQ=60)
      CGCTACAGGCGAAATATGGTGCTGGCAGGAAGCTAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                >  SRR4302532.2973541/1‑94 (MQ=60)
      CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                >  SRR4302532.1349380/1‑94 (MQ=60)
       GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCG                                                                               <  SRR4302532.1493166/94‑1 (MQ=60)
       GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCG                                                                               <  SRR4302532.4202236/94‑1 (MQ=60)
        CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              <  SRR4302532.5235996/94‑1 (MQ=60)
        CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              >  SRR4302532.3593117/1‑94 (MQ=60)
        CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              <  SRR4302532.1557869/94‑1 (MQ=60)
        CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              <  SRR4302532.1557871/94‑1 (MQ=60)
         TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGA                                                                             <  SRR4302532.4038022/94‑1 (MQ=60)
          ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302532.2452537/94‑1 (MQ=60)
          ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302532.5345462/94‑1 (MQ=60)
           CAGGCGAAATATGGTGCTGGCAGGAAGATAAGTCACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGC                                                                           >  SRR4302532.6099260/1‑94 (MQ=60)
                GAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAT                                                                      <  SRR4302532.1650102/94‑1 (MQ=60)
                GAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAT                                                                      >  SRR4302532.1610038/1‑94 (MQ=60)
                GAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAT                                                                      >  SRR4302532.3474978/1‑94 (MQ=60)
                 GAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAGCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATT                                                                     <  SRR4302532.5827686/94‑1 (MQ=60)
                  AATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTT                                                                    >  SRR4302532.4079251/1‑94 (MQ=60)
                  AATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTT                                                                    <  SRR4302532.2116190/94‑1 (MQ=60)
                   ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   <  SRR4302532.921393/94‑1 (MQ=60)
                    TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  >  SRR4302532.413704/1‑94 (MQ=60)
                     ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 <  SRR4302532.2789116/94‑1 (MQ=60)
                      TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTT                                                                <  SRR4302532.3035560/94‑1 (MQ=60)
                      TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTT                                                                <  SRR4302532.5061849/94‑1 (MQ=60)
                       GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               <  SRR4302532.849105/94‑1 (MQ=60)
                         TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             <  SRR4302532.3237595/94‑1 (MQ=60)
                         TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             <  SRR4302532.3336628/94‑1 (MQ=60)
                          GCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTG                                                            <  SRR4302532.2826230/94‑1 (MQ=60)
                          GCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTG                                                            <  SRR4302532.5708746/94‑1 (MQ=60)
                          GCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTG                                                            <  SRR4302532.615445/94‑1 (MQ=60)
                           CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           >  SRR4302532.3262353/1‑94 (MQ=60)
                            gcGCAGGAAGGTAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCGCGCGGAGCATCACTTTCTTGTTGGT                                                          <  SRR4302532.888491/92‑1 (MQ=60)
                            TGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGT                                                          <  SRR4302532.888490/94‑1 (MQ=60)
                              GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        <  SRR4302532.398049/94‑1 (MQ=60)
                              GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        <  SRR4302532.1622555/94‑1 (MQ=60)
                               GAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       <  SRR4302532.4792412/94‑1 (MQ=60)
                               CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       <  SRR4302532.371265/94‑1 (MQ=60)
                                AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302532.2180528/1‑94 (MQ=60)
                                AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      <  SRR4302532.3492703/94‑1 (MQ=60)
                                 GTAAGATAAGTAAAAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTTCGCGTTTCTCCCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302532.458668/94‑1 (MQ=60)
                                 GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     >  SRR4302532.1461820/1‑94 (MQ=60)
                                 GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302532.3787143/94‑1 (MQ=60)
                                 GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302532.3145218/94‑1 (MQ=60)
                                  GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302532.1377790/94‑1 (MQ=60)
                                  GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302532.4824736/94‑1 (MQ=60)
                                  GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    >  SRR4302532.3433865/1‑94 (MQ=60)
                                   AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   <  SRR4302532.4279426/94‑1 (MQ=60)
                                   AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   <  SRR4302532.5526140/94‑1 (MQ=60)
                                    AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  >  SRR4302532.5833870/1‑94 (MQ=60)
                                    AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  <  SRR4302532.376157/94‑1 (MQ=60)
                                    AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  <  SRR4302532.4436970/94‑1 (MQ=60)
                                     GATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 <  SRR4302532.815229/94‑1 (MQ=60)
                                     GATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 >  SRR4302532.5751477/1‑94 (MQ=60)
                                      ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                >  SRR4302532.3093542/1‑94 (MQ=60)
                                      ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                >  SRR4302532.1928721/1‑94 (MQ=60)
                                       TAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGA                                               <  SRR4302532.6321081/94‑1 (MQ=60)
                                       TAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGA                                               <  SRR4302532.486789/94‑1 (MQ=60)
                                        AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302532.4486893/94‑1 (MQ=60)
                                        AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302532.5606438/94‑1 (MQ=60)
                                        AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302532.1192876/94‑1 (MQ=60)
                                        AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              >  SRR4302532.4157469/1‑94 (MQ=60)
                                         AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             <  SRR4302532.913567/94‑1 (MQ=60)
                                         AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             <  SRR4302532.4870668/94‑1 (MQ=60)
                                          GTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGG                                            >  SRR4302532.1524964/1‑94 (MQ=60)
                                           TAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGC                                           <  SRR4302532.2694031/94‑1 (MQ=60)
                                            AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGTC                                          >  SRR4302532.4150753/1‑94 (MQ=60)
                                            AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGGGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302532.4150899/1‑94 (MQ=60)
                                             ACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCC                                         <  SRR4302532.760204/94‑1 (MQ=60)
                                              CAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCG                                        <  SRR4302532.5560969/94‑1 (MQ=60)
                                               AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTTGCGGAGCATCATTTTCTTGTTTGTTTATCGCTTGATGGCCCTC                                       >  SRR4302532.5839365/1‑94 (MQ=60)
                                               AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGC                                       >  SRR4302532.6038930/1‑94 (MQ=60)
                                               AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGC                                       >  SRR4302532.4902753/1‑94 (MQ=60)
                                                 CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     >  SRR4302532.1085401/1‑94 (MQ=60)
                                                  TTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTG                                    >  SRR4302532.2343839/1‑94 (MQ=60)
                                                  TTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTG                                    >  SRR4302532.4909368/1‑94 (MQ=60)
                                                   TCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGACCCGCCTGA                                   >  SRR4302532.2963074/1‑94 (MQ=60)
                                                    CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  >  SRR4302532.1081439/1‑94 (MQ=60)
                                                     CAGACTAACGGCGGGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATGTTCTTGTTGGTGTATCGCTGGATGGCCCGCCTGAGA                                 >  SRR4302532.5673656/1‑94 (MQ=60)
                                                      AGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAT                                <  SRR4302532.3274340/94‑1 (MQ=60)
                                                      AGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAT                                >  SRR4302532.3233741/1‑94 (MQ=60)
                                                        ACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCC                              >  SRR4302532.1908792/1‑94 (MQ=60)
                                                         CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             <  SRR4302532.1001447/94‑1 (MQ=60)
                                                          TAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAC                            <  SRR4302532.427685/94‑1 (MQ=60)
                                                          TAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCCGAGATCCAC                            >  SRR4302532.6243364/1‑94 (MQ=60)
                                                           AACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACA                           <  SRR4302532.6042360/94‑1 (MQ=60)
                                                             CGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAAT                         >  SRR4302532.5754481/1‑94 (MQ=60)
                                                              GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        <  SRR4302532.3672219/94‑1 (MQ=60)
                                                                CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCCGCGATCCACCATCAA                      >  SRR4302532.1453352/1‑94 (MQ=60)
                                                                 GTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAAT                     >  SRR4302532.1022653/1‑94 (MQ=60)
                                                                  TGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATA                    >  SRR4302532.3065297/1‑94 (MQ=60)
                                                                    CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCCTTTTCTTGTTGGTTTATCGCTGGCTGGCGCGCCTGAGCTCCACCATCAATATC                  >  SRR4302532.2253123/1‑94 (MQ=60)
                                                                    CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCCGAGATCCACAATCAATATC                  >  SRR4302532.3668859/1‑94 (MQ=60)
                                                                     TGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 >  SRR4302532.5262125/1‑94 (MQ=60)
                                                                     TGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 <  SRR4302532.2742303/94‑1 (MQ=60)
                                                                     TGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 <  SRR4302532.3088226/94‑1 (MQ=60)
                                                                       CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               <  SRR4302532.196048/94‑1 (MQ=60)
                                                                        TGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGT              >  SRR4302532.6105076/1‑94 (MQ=60)
                                                                        TGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGT              <  SRR4302532.185011/94‑1 (MQ=60)
                                                                         GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             <  SRR4302532.3014240/94‑1 (MQ=60)
                                                                         GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             <  SRR4302532.5041729/94‑1 (MQ=60)
                                                                          GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGA            <  SRR4302532.5107110/94‑1 (MQ=60)
                                                                          GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGA            <  SRR4302532.49664/94‑1 (MQ=60)
                                                                            TGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACT          <  SRR4302532.3278152/94‑1 (MQ=60)
                                                                            TGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACT          <  SRR4302532.903279/94‑1 (MQ=60)
                                                                             GACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTA         <  SRR4302532.442458/94‑1 (MQ=60)
                                                                             GACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTA         >  SRR4302532.1641672/1‑94 (MQ=60)
                                                                              ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        <  SRR4302532.861281/94‑1 (MQ=60)
                                                                               CGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAA       <  SRR4302532.2439694/94‑1 (MQ=60)
                                                                                GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302532.3403253/94‑1 (MQ=60)
                                                                                GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302532.6251434/94‑1 (MQ=60)
                                                                                GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      >  SRR4302532.4931986/1‑94 (MQ=60)
                                                                                 AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     <  SRR4302532.29984/94‑1 (MQ=60)
                                                                                   TGGTGCGCGTTTCTTGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302532.4695797/94‑1 (MQ=60)
                                                                                   TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302532.1199373/94‑1 (MQ=60)
                                                                                   TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302532.2149791/94‑1 (MQ=60)
                                                                                   TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302532.3037203/94‑1 (MQ=60)
                                                                                    GGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  <  SRR4302532.735706/94‑1 (MQ=60)
                                                                                        |                                                                                         
CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980830‑3981007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑