Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,918 2 bp→CG coding (348‑349/1236 nt) aslB → putative arylsulfatase regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9180GC100.0% 934.7 / NA 252W116C (TGG→TGC) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (125/127);  total (125/127)
*NC_0009133,980,9190CG99.6% 922.7 / ‑5.5 255P117A (CCG→GCG) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (124/130);  minor base A (1/0);  total (125/130)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 259 total aligned reads displayed.

CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005
                                                                                       ||                                                                                      
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  2:4107453/1‑93 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  2:3581088/1‑93 (MQ=255)
  tAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc                                                                                  <  2:6635761/93‑1 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  1:6048722/93‑1 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  2:2697524/93‑1 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  1:4219068/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTTCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  1:567106/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  2:2381112/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  2:6417847/93‑1 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              <  2:5024819/93‑1 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             >  2:4590712/1‑93 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             <  1:3040669/93‑1 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  1:1924179/93‑1 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           >  2:4788510/1‑93 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           >  1:3871355/1‑93 (MQ=255)
           aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc                                                                         >  2:2015848/1‑93 (MQ=255)
           aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc                                                                         <  2:5649419/93‑1 (MQ=255)
           aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc                                                                         >  2:2436221/1‑93 (MQ=255)
            ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                        >  1:5822004/1‑93 (MQ=255)
            ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                        >  1:3943401/1‑93 (MQ=255)
            ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                        >  1:4151633/1‑93 (MQ=255)
             gCGAAATATTGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                       <  2:3736111/93‑1 (MQ=255)
             gCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                       <  2:5464888/93‑1 (MQ=255)
               gAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                     >  2:3369169/1‑93 (MQ=255)
               gAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                     >  1:6124663/1‑93 (MQ=255)
                aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                    >  2:6007171/1‑93 (MQ=255)
                aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                    >  2:613533/1‑93 (MQ=255)
                 aaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                   <  1:2673315/93‑1 (MQ=255)
                   tatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt                                                                 <  1:3185772/93‑1 (MQ=255)
                   tatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt                                                                 >  1:1085544/1‑93 (MQ=255)
                   tatGGTGCTCGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt                                                                 >  2:4613433/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               >  1:922554/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               >  2:2236627/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTCCGCGTTTCTCGCGGAGCATCATTTTCt                                                               >  1:4844755/1‑93 (MQ=255)
                      ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt                                                              <  1:3967121/93‑1 (MQ=255)
                      ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt                                                              >  1:6528900/1‑93 (MQ=255)
                        tGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgt                                                            <  2:162531/93‑1 (MQ=255)
                            ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                        <  1:1273411/93‑1 (MQ=255)
                             gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                       <  2:468379/93‑1 (MQ=255)
                             gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                       <  1:3572337/93‑1 (MQ=255)
                              cAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGttt                                                      <  2:5474431/93‑1 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtttt                                                     >  1:988839/1‑92 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     <  2:4183896/93‑1 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     >  2:2127947/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    >  2:5697467/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    >  1:2119470/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    >  1:142638/1‑93 (MQ=255)
                                  aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                  <  1:2222102/93‑1 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc                                                 >  1:3618946/1‑93 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc                                                 >  2:450615/1‑93 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              <  2:1268891/93‑1 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  2:3794140/1‑93 (MQ=255)
                                       aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                             <  1:2787928/93‑1 (MQ=255)
                                       aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                             <  2:6093625/93‑1 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                           >  2:1764223/1‑93 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                           <  1:4793894/93‑1 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                           >  1:5470944/1‑93 (MQ=255)
                                           aaCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCCGGATGGc                                         <  1:5372234/93‑1 (MQ=255)
                                            aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc                                        >  2:3444208/1‑93 (MQ=255)
                                              aGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCg                                      >  2:5370385/1‑93 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    >  2:767464/1‑93 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    <  2:2173116/93‑1 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    <  2:1119073/93‑1 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    >  2:2995860/1‑93 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGGCCGCCt                                   >  1:2328390/1‑93 (MQ=255)
                                                   ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTga                                 >  2:3979315/1‑93 (MQ=255)
                                                   ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCACTGGATGGCCCGCCTga                                 >  2:628277/1‑93 (MQ=255)
                                                      gACTAACGGCGTGCTGCTGGATGACGAATGGTGCACGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAt                              >  2:6344639/1‑93 (MQ=255)
                                                       aCTAACGGCGTGCTGCTGGATGACGAATGGTTCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATc                             <  1:6307860/93‑1 (MQ=255)
                                                        cTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc                            <  2:5629435/93‑1 (MQ=255)
                                                        cTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc                            <  1:2855909/93‑1 (MQ=255)
                                                        cTAACGGCGTGCTGCTGGATGACGAATGGGGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc                            >  2:3188855/1‑93 (MQ=255)
                                                            cGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaa                        <  1:4278537/93‑1 (MQ=255)
                                                            cGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaa                        <  1:246513/93‑1 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc                      >  2:2922132/1‑93 (MQ=255)
                                                               cGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatca                     <  2:5195249/93‑1 (MQ=255)
                                                                gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaa                    >  2:1310955/1‑93 (MQ=255)
                                                                gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaa                    >  1:180889/1‑93 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  >  1:5122691/1‑93 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  >  2:4348515/1‑93 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  <  1:4550416/93‑1 (MQ=255)
                                                                   ctgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAatat                 >  1:1658595/1‑93 (MQ=255)
                                                                   ctgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAatat                 <  1:1397505/93‑1 (MQ=255)
                                                                   ctgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAatat                 >  2:1939921/1‑93 (MQ=255)
                                                                     gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcg               >  2:1436348/1‑93 (MQ=255)
                                                                      ctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcgc              >  2:5226957/1‑93 (MQ=255)
                                                                        ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  1:6166652/93‑1 (MQ=255)
                                                                        ggATCACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  2:4938128/93‑1 (MQ=255)
                                                                         gATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTg           >  1:4608323/1‑93 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         <  1:3689276/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       >  2:5272798/1‑93 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  2:2589360/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  1:1633515/93‑1 (MQ=255)
                                                                              cGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaa      >  2:2839088/1‑93 (MQ=255)
                                                                               gAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaaa     >  2:4745717/1‑93 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    >  2:5085644/1‑93 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    >  1:6290679/1‑93 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    <  2:3216561/93‑1 (MQ=255)
                                                                                 aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAgg   <  1:5893883/93‑1 (MQ=255)
                                                                                 aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAgg   <  1:687505/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980825‑3981007
                                                                                             |                                                                                         
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGC                                                                                           <  SRR4302547.6739118/94‑1 (MQ=60)
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGC                                                                                           <  SRR4302547.2961774/94‑1 (MQ=60)
      CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     <  SRR4302547.2355382/94‑1 (MQ=60)
      CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     >  SRR4302547.1659841/1‑94 (MQ=60)
       GTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTC                                                                                    <  SRR4302547.2718575/94‑1 (MQ=60)
       GTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTACTGGATGACGAATGGTGCGCGTTTC                                                                                    <  SRR4302547.6538226/94‑1 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302547.6174216/94‑1 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302547.4305229/94‑1 (MQ=60)
          GCGCTACAGGCGAAATATGGTTCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCG                                                                                 <  SRR4302547.577299/94‑1 (MQ=60)
            GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCG                                                                               >  SRR4302547.6151051/1‑94 (MQ=60)
             CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              <  SRR4302547.3101189/94‑1 (MQ=60)
              TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGG                                                                             >  SRR4302547.2587371/1‑94 (MQ=60)
              TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGA                                                                             >  SRR4302547.2800397/1‑94 (MQ=60)
              TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGA                                                                             >  SRR4302547.3950155/1‑94 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302547.4624554/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302547.1962294/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAA                                                                            <  SRR4302547.1928334/94‑1 (MQ=60)
                 AGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCA                                                                          >  SRR4302547.4023601/1‑94 (MQ=60)
                 AGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCA                                                                          >  SRR4302547.4236194/1‑94 (MQ=60)
                 AGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCA                                                                          >  SRR4302547.5942450/1‑94 (MQ=60)
                 AGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCCGAGCA                                                                          >  SRR4302547.6292208/1‑94 (MQ=60)
                   GCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATC                                                                        <  SRR4302547.6027512/94‑1 (MQ=60)
                    CGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCA                                                                       >  SRR4302547.6251719/1‑94 (MQ=60)
                    CGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCA                                                                       <  SRR4302547.6691726/94‑1 (MQ=60)
                    CGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCA                                                                       <  SRR4302547.5578671/94‑1 (MQ=60)
                       AATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTT                                                                    <  SRR4302547.2727016/94‑1 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   >  SRR4302547.1107233/1‑94 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  <  SRR4302547.3249243/94‑1 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  <  SRR4302547.6187336/94‑1 (MQ=60)
                          ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 >  SRR4302547.940376/1‑94 (MQ=60)
                          ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 >  SRR4302547.2391231/1‑94 (MQ=60)
                          ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTCCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 >  SRR4302547.4945152/1‑94 (MQ=60)
                           TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTT                                                                >  SRR4302547.872080/1‑94 (MQ=60)
                           TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTT                                                                >  SRR4302547.6664796/1‑94 (MQ=60)
                           TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTCGATGACGAATGGTGGGCGTTTCTCGCGGAGGATGATTTTCTT                                                                >  SRR4302547.4921385/1‑94 (MQ=60)
                            GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               <  SRR4302547.4047814/94‑1 (MQ=60)
                            GGTGCTGCCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTGCTTG                                                               <  SRR4302547.1377388/94‑1 (MQ=60)
                             GTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGT                                                              >  SRR4302547.5175883/1‑94 (MQ=60)
                             GTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGAGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGT                                                              <  SRR4302547.6285474/94‑1 (MQ=60)
                              TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             <  SRR4302547.5832742/94‑1 (MQ=60)
                                CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           <  SRR4302547.1348705/94‑1 (MQ=60)
                                CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           <  SRR4302547.1961155/94‑1 (MQ=60)
                                  GGCAGTAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTT                                                         <  SRR4302547.3323906/94‑1 (MQ=60)
                                  GGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTT                                                         <  SRR4302547.1299060/94‑1 (MQ=60)
                                   GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        <  SRR4302547.4058488/94‑1 (MQ=60)
                                   GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        <  SRR4302547.3645210/94‑1 (MQ=60)
                                   GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGCGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        <  SRR4302547.442616/94‑1 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTT                                                       >  SRR4302547.1008088/1‑94 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       <  SRR4302547.2236554/94‑1 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302547.2161716/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      <  SRR4302547.966367/94‑1 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302547.5460474/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302547.145218/1‑94 (MQ=60)
                                      GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATG                                                     >  SRR4302547.4645943/1‑94 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302547.1648099/94‑1 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302547.400784/94‑1 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   >  SRR4302547.3692773/1‑94 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   >  SRR4302547.3062690/1‑94 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   <  SRR4302547.2266867/94‑1 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  <  SRR4302547.4303305/94‑1 (MQ=60)
                                          GATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 >  SRR4302547.6424006/1‑94 (MQ=60)
                                             AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302547.2843764/94‑1 (MQ=60)
                                             AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302547.1727869/94‑1 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             >  SRR4302547.2509132/1‑94 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             >  SRR4302547.5583963/1‑94 (MQ=60)
                                               GTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGG                                            <  SRR4302547.1798547/94‑1 (MQ=60)
                                               GTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGG                                            <  SRR4302547.4893231/94‑1 (MQ=60)
                                               GTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGG                                            <  SRR4302547.5274643/94‑1 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302547.4788840/1‑94 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          <  SRR4302547.3107554/94‑1 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCCGGATGGCC                                          <  SRR4302547.5483105/94‑1 (MQ=60)
                                                    AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGC                                       <  SRR4302547.486948/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGGCCGCCT                                     >  SRR4302547.2375584/1‑94 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     <  SRR4302547.3125756/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     >  SRR4302547.5775673/1‑94 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     >  SRR4302547.2022457/1‑94 (MQ=60)
                                                        TCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGA                                   <  SRR4302547.5950209/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  >  SRR4302547.3659478/1‑94 (MQ=60)
                                                          CAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGA                                 >  SRR4302547.6355828/1‑94 (MQ=60)
                                                           AGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAT                                >  SRR4302547.4928831/1‑94 (MQ=60)
                                                             ACTAACGGCGTGCTGCTGGATGACGAATGGTTCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCC                              <  SRR4302547.6439005/94‑1 (MQ=60)
                                                             ACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCC                              <  SRR4302547.2373131/94‑1 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             >  SRR4302547.1393177/1‑94 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             >  SRR4302547.4046749/1‑94 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             <  SRR4302547.2912976/94‑1 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             >  SRR4302547.1677615/1‑94 (MQ=60)
                                                               TAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAC                            <  SRR4302547.5677735/94‑1 (MQ=60)
                                                                AACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACA                           >  SRR4302547.814760/1‑94 (MQ=60)
                                                                 ACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAA                          >  SRR4302547.1292400/1‑94 (MQ=60)
                                                                  CGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAAT                         <  SRR4302547.251118/94‑1 (MQ=60)
                                                                  CGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAAT                         <  SRR4302547.4366409/94‑1 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        >  SRR4302547.4325989/1‑94 (MQ=60)
                                                                    GCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCA                       <  SRR4302547.3744130/94‑1 (MQ=60)
                                                                     CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAA                      >  SRR4302547.184288/1‑94 (MQ=60)
                                                                       TGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATA                    >  SRR4302547.5228247/1‑94 (MQ=60)
                                                                        GCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   >  SRR4302547.1691312/1‑94 (MQ=60)
                                                                        GCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   <  SRR4302547.4644533/94‑1 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATC                  <  SRR4302547.1425463/94‑1 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               <  SRR4302547.5902788/94‑1 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               <  SRR4302547.2901571/94‑1 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               >  SRR4302547.2874905/1‑94 (MQ=60)
                                                                             TGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGT              <  SRR4302547.4298124/94‑1 (MQ=60)
                                                                              GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             >  SRR4302547.2245340/1‑94 (MQ=60)
                                                                              GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             <  SRR4302547.6294645/94‑1 (MQ=60)
                                                                              GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             >  SRR4302547.4703696/1‑94 (MQ=60)
                                                                               GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGA            >  SRR4302547.371102/1‑94 (MQ=60)
                                                                                 TGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACT          <  SRR4302547.3764590/94‑1 (MQ=60)
                                                                                  GACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTA         <  SRR4302547.3370482/94‑1 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        <  SRR4302547.4097340/94‑1 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        <  SRR4302547.1665836/94‑1 (MQ=60)
                                                                                    CGAATGGTGCGCGTTGCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAA       <  SRR4302547.2607700/94‑1 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302547.6334141/94‑1 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      >  SRR4302547.6421407/1‑94 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     >  SRR4302547.1834221/1‑94 (MQ=60)
                                                                                       ATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT    <  SRR4302547.6015909/94‑1 (MQ=60)
                                                                                       ATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT    <  SRR4302547.700193/94‑1 (MQ=60)
                                                                                       ATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT    >  SRR4302547.5060507/1‑94 (MQ=60)
                                                                                       ATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT    <  SRR4302547.3969314/94‑1 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302547.3929943/94‑1 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   >  SRR4302547.6213218/1‑94 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302547.6628337/94‑1 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302547.1450091/94‑1 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATAAATATCGCGTTACTAAAGGTG   >  SRR4302547.4894366/1‑94 (MQ=60)
                                                                                         GGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  <  SRR4302547.3913501/94‑1 (MQ=60)
                                                                                         GGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  <  SRR4302547.2577357/94‑1 (MQ=60)
                                                                                             |                                                                                         
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980825‑3981007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑