Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,519 +T intergenic (‑268/‑530) cpxR ← / → yiiP transcriptional regulator in 2‑component system/putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5191.T100.0% 715.9 / NA 193intergenic (‑268/‑530)cpxR/yiiPtranscriptional regulator in 2‑component system/putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (93/100);  total (93/100)

Only 100 of 242 total aligned reads displayed.

GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605
                                                                                             |                                                                                      
gtccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcag                                                                                         <  2:6039347/91‑1 (MQ=255)
ggCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcag                                                                                         <  2:672875/93‑1 (MQ=255)
   cTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtac                                                                                      <  2:1053236/93‑4 (MQ=255)
   cTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtac                                                                                      <  1:3016856/93‑4 (MQ=255)
   cTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtac                                                                                      <  1:5734465/93‑4 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  2:5947194/1‑89 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  2:5792366/1‑89 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  2:4515952/1‑89 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  >  1:3763799/1‑93 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  >  1:2223698/1‑93 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  >  2:4218898/1‑93 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcaga                                                                                 <  2:4813422/93‑1 (MQ=255)
         gCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCagag                                                                                >  1:3108853/1‑93 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  1:2877338/1‑93 (MQ=255)
            ggCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCa                                                                             >  2:2747076/1‑93 (MQ=255)
             gCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCat                                                                            <  1:2664623/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           <  1:4376136/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           >  2:2894265/1‑93 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          >  1:4990560/1‑93 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          >  1:3958650/1‑93 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGt                                                                        >  1:5373002/1‑93 (MQ=255)
                  cagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGtt                                                                       <  2:2464016/93‑1 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      >  1:1184956/1‑93 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      >  1:894499/1‑93 (MQ=255)
                    gtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCg                                                                     >  2:5995428/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGCTCGa                                                                    >  1:974581/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGAACGCAGAGCCATATGTTCGa                                                                    >  1:1374847/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGATGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGa                                                                    <  1:3304707/93‑1 (MQ=255)
                      tCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAc                                                                   <  2:1137757/93‑1 (MQ=255)
                      tCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAc                                                                   <  1:2099775/93‑1 (MQ=255)
                       cATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACg                                                                  <  2:3620535/93‑1 (MQ=255)
                       cATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACg                                                                  <  2:1677771/93‑1 (MQ=255)
                        aTTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACgg                                                                 >  2:5033531/1‑93 (MQ=255)
                           aaGCCACGCTGCTGAATTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAt                                                              <  1:321203/93‑1 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATaa                                                            <  2:3000638/93‑1 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATaa                                                            >  2:5646209/1‑93 (MQ=255)
                                 cGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGttt                                                        <  1:605516/93‑1 (MQ=255)
                                 cGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGttt                                                        <  2:4878443/93‑1 (MQ=255)
                                  gctgctGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTa                                                       >  2:1320157/1‑93 (MQ=255)
                                   ctgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTaa                                                      <  1:3663015/93‑1 (MQ=255)
                                   ctgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTaa                                                      >  1:348193/1‑93 (MQ=255)
                                   cggctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTaa                                                      >  2:3780560/3‑93 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  1:5119573/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  1:1068079/93‑1 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    <  2:828897/93‑1 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    <  1:5914418/93‑1 (MQ=255)
                                      ctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCg                                                   >  2:463873/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  1:620677/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  2:335290/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  1:5238813/1‑93 (MQ=255)
                                         aaGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAc                                                <  2:3953865/93‑1 (MQ=255)
                                         aaGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAc                                                >  1:1934782/1‑93 (MQ=255)
                                         aaGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAc                                                <  2:5386332/93‑1 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACa                                               <  1:5819114/93‑1 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACa                                               >  2:5086457/1‑93 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACa                                               >  2:5536455/1‑93 (MQ=255)
                                           gTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAt                                              <  1:1832906/93‑1 (MQ=255)
                                             cGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCa                                            <  2:2423255/93‑1 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAgg                                          >  1:1368779/1‑92 (MQ=255)
                                                ttCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCg                                         >  1:4254638/1‑93 (MQ=255)
                                                 tCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGt                                        <  2:1538060/93‑1 (MQ=255)
                                                   aGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTca                                      >  2:1888770/1‑93 (MQ=255)
                                                   aGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTca                                      <  2:3918098/93‑1 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     >  2:4693574/1‑93 (MQ=255)
                                                     gCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagc                                    >  1:1138859/1‑93 (MQ=255)
                                                     gCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagc                                    >  1:1701273/1‑93 (MQ=255)
                                                      cGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagca                                   <  2:4129773/93‑1 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  2:414081/93‑1 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 >  2:4947483/1‑93 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  1:2439251/93‑1 (MQ=255)
                                                         tAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAt                                <  1:2018005/93‑1 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              <  1:5517287/93‑1 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              <  1:2448163/93‑1 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            >  2:1793447/1‑93 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            >  2:3288066/1‑93 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            <  1:2163194/93‑1 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            >  1:4893988/1‑93 (MQ=255)
                                                               gCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgaga                          <  1:5260073/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAtt                        >  2:1432752/1‑92 (MQ=255)
                                                                    caggTGTAGAACTTACGCAGCGCAGTACGAAGAGCCATATGTACGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCAAGTTCTTa                     <  1:5496377/91‑1 (MQ=255)
                                                                    cGGGTGAAGATCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     <  1:2039401/93‑1 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     >  2:2341799/1‑93 (MQ=255)
                                                                       gTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGc                  <  1:1050779/93‑1 (MQ=255)
                                                                       gTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGc                  <  2:1446759/93‑1 (MQ=255)
                                                                        tGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCa                 >  1:3593194/1‑93 (MQ=255)
                                                                         gaagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaa                >  1:5984007/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  2:3125/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  1:2489078/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  2:7199/1‑93 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              >  1:83962/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  2:2176627/93‑1 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  1:1201912/93‑1 (MQ=255)
                                                                              aCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGc           >  1:3320796/1‑93 (MQ=255)
                                                                               cTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGcc          >  1:737647/1‑93 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     <  2:434737/93‑1 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     >  1:150855/1‑93 (MQ=255)
                                                                                     cagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAc    <  1:6075271/93‑1 (MQ=255)
                                                                                      agcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACg   <  2:35099/93‑1 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  >  1:1341259/1‑93 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  >  2:6147388/1‑93 (MQ=255)
                                                                                             |                                                                                      
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG  >  NC_000913/4103426‑4103609
                                                                                             |                                                                                           
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                             <  SRR4302532.3251161/94‑1 (MQ=60)
 GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                           <  SRR4302532.783689/94‑1 (MQ=60)
 GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                           <  SRR4302532.3362216/94‑1 (MQ=60)
 GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                           >  SRR4302532.1192651/1‑94 (MQ=60)
   CCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGtac                                                                                          >  SRR4302532.2044960/1‑91 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                         >  SRR4302532.3141689/1‑94 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                         >  SRR4302532.2207008/1‑94 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                         <  SRR4302532.3116403/94‑1 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                         <  SRR4302532.5934142/94‑1 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGTTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                      <  SRR4302532.3071613/94‑1 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                      >  SRR4302532.3890451/1‑94 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                      <  SRR4302532.3573379/94‑1 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                      >  SRR4302532.2297515/1‑94 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                      <  SRR4302532.1996131/94‑1 (MQ=60)
         CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                    <  SRR4302532.6349519/94‑1 (MQ=60)
         CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                    >  SRR4302532.3211978/1‑94 (MQ=60)
           CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                  >  SRR4302532.2973553/1‑94 (MQ=60)
           CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                  >  SRR4302532.3242722/1‑94 (MQ=60)
              GCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATA                                                                               <  SRR4302532.2754055/94‑1 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                              >  SRR4302532.4092041/1‑94 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                              >  SRR4302532.5161614/1‑94 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                              <  SRR4302532.4524694/94‑1 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                              <  SRR4302532.2675490/94‑1 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             <  SRR4302532.5984693/94‑1 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             >  SRR4302532.1762754/1‑94 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             >  SRR4302532.2478365/1‑94 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             >  SRR4302532.1619903/1‑94 (MQ=60)
                 GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                            >  SRR4302532.5559163/1‑94 (MQ=60)
                  TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                           <  SRR4302532.5882608/94‑1 (MQ=60)
                  TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                           <  SRR4302532.277508/94‑1 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                          >  SRR4302532.6140886/1‑94 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                          >  SRR4302532.1224560/1‑94 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                          >  SRR4302532.1330307/1‑94 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                          >  SRR4302532.925028/1‑94 (MQ=60)
                    AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                         <  SRR4302532.528513/94‑1 (MQ=60)
                     GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                        >  SRR4302532.1420903/1‑94 (MQ=60)
                     GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                        >  SRR4302532.2091699/1‑94 (MQ=60)
                     GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGCTCGA                                                                        >  SRR4302532.1007849/1‑94 (MQ=60)
                     GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGAACGCAGAGCCATATGTTCGA                                                                        >  SRR4302532.1420904/1‑94 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                       <  SRR4302532.5093603/94‑1 (MQ=60)
                      TTCATTAAGCCACGCTGATGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                       <  SRR4302532.3414736/94‑1 (MQ=60)
                       TCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACG                                                                      <  SRR4302532.2169827/94‑1 (MQ=60)
                        CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGG                                                                     >  SRR4302532.6318659/1‑94 (MQ=60)
                         ATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGC                                                                    >  SRR4302532.5183433/1‑94 (MQ=60)
                         ATTAACCCAGGCTGCTGAAGTCGGTTCATGCTATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGC                                                                    <  SRR4302532.5311450/94‑1 (MQ=60)
                            AAGCCACGCTGCTGAATTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATA                                                                 <  SRR4302532.332359/94‑1 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                               <  SRR4302532.1224232/94‑1 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                               <  SRR4302532.5700871/94‑1 (MQ=60)
                                CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTT                                                             <  SRR4302532.4075103/94‑1 (MQ=60)
                                 ACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTT                                                            <  SRR4302532.1112685/94‑1 (MQ=60)
                                  cgcgaCTGAAGTCGGTGCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                           <  SRR4302532.3914479/89‑1 (MQ=60)
                                  CGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                           <  SRR4302532.626670/94‑1 (MQ=60)
                                  CGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                           <  SRR4302532.626671/94‑1 (MQ=60)
                                  CGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                           <  SRR4302532.3914478/94‑1 (MQ=60)
                                   GCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAA                                                          >  SRR4302532.360253/1‑94 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                         <  SRR4302532.3786008/94‑1 (MQ=60)
                                     TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                        <  SRR4302532.5295657/94‑1 (MQ=60)
                                     TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                        <  SRR4302532.1103985/94‑1 (MQ=60)
                                     TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                        <  SRR4302532.756140/94‑1 (MQ=60)
                                      GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                       <  SRR4302532.6119224/94‑1 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                      >  SRR4302532.5419440/1‑94 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                      >  SRR4302532.642321/1‑94 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                      >  SRR4302532.5519312/1‑94 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                      >  SRR4302532.3859972/1‑94 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                      >  SRR4302532.2211455/1‑94 (MQ=60)
                                        TGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAA                                                     >  SRR4302532.815258/1‑94 (MQ=60)
                                        TGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGGTTAACCGAA                                                     >  SRR4302532.5699919/1‑94 (MQ=60)
                                         GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                    >  SRR4302532.1999171/1‑94 (MQ=60)
                                           AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                  <  SRR4302532.6021223/94‑1 (MQ=60)
                                            GTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                 <  SRR4302532.1893866/94‑1 (MQ=60)
                                            GTCGGGTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                 <  SRR4302532.2316359/94‑1 (MQ=60)
                                              CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                               <  SRR4302532.2545596/94‑1 (MQ=60)
                                               GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGG                                              >  SRR4302532.1414631/1‑94 (MQ=60)
                                               GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGC                                              <  SRR4302532.3755724/94‑1 (MQ=60)
                                                GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                             >  SRR4302532.4397671/1‑94 (MQ=60)
                                                GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                             >  SRR4302532.3708004/1‑94 (MQ=60)
                                                   CAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                          <  SRR4302532.1327642/94‑1 (MQ=60)
                                                   CAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                          >  SRR4302532.4476834/1‑94 (MQ=60)
                                                   CAGGCGATAACTGGCATCCGGGTAAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                          <  SRR4302532.4644127/94‑1 (MQ=60)
                                                    AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                         >  SRR4302532.6245394/1‑94 (MQ=60)
                                                     GGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGC                                        >  SRR4302532.1176830/1‑94 (MQ=60)
                                                     GGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGC                                        >  SRR4302532.1758657/1‑94 (MQ=60)
                                                         ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                    <  SRR4302532.3238847/94‑1 (MQ=60)
                                                         ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                    <  SRR4302532.2520353/94‑1 (MQ=60)
                                                         ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                    >  SRR4302532.2107919/1‑94 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGCGCCTTATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                   <  SRR4302532.5686317/94‑1 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                   <  SRR4302532.2085288/94‑1 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                   <  SRR4302532.1099037/94‑1 (MQ=60)
                                                            ACTGTCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATATTCGGCGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                 <  SRR4302532.6041322/94‑1 (MQ=60)
                                                            ACTGGCATCCGGGTGAAGACCTTACGCCGCGCAGTACGCAGAGCCATATGTTCGAAGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCA                                 <  SRR4302532.2529565/94‑1 (MQ=60)
                                                            ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                 <  SRR4302532.5708736/94‑1 (MQ=60)
                                                            ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                 <  SRR4302532.2529566/94‑1 (MQ=60)
                                                             CTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGA                                >  SRR4302532.5062154/1‑94 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                               <  SRR4302532.2235533/94‑1 (MQ=60)
                                                                GCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAT                             <  SRR4302532.5441454/94‑1 (MQ=60)
                                                                 CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                            <  SRR4302532.2678687/94‑1 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                           >  SRR4302532.3077415/1‑94 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                           <  SRR4302532.5993354/94‑1 (MQ=60)
                                                                     CGGGTGAAGATCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                        <  SRR4302532.2107339/94‑1 (MQ=60)
                                                                     CAGGTGTAGAACTTACGCAGCGCAGTACGAAGAGCCATATGTACGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCAAGTTCTTAT                        <  SRR4302532.5687000/94‑1 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                       >  SRR4302532.2013766/1‑94 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                       <  SRR4302532.4299200/94‑1 (MQ=60)
                                                                       GGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGC                      <  SRR4302532.5513150/94‑1 (MQ=60)
                                                                       GGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGC                      <  SRR4302532.3415996/94‑1 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                     <  SRR4302532.1086206/94‑1 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                     >  SRR4302532.3713365/1‑94 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                     >  SRR4302532.229151/1‑94 (MQ=60)
                                                                         TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302532.6190763/1‑94 (MQ=60)
                                                                         TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302532.6190765/1‑94 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                   >  SRR4302532.2572030/1‑94 (MQ=60)
                                                                           AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                  >  SRR4302532.86935/1‑94 (MQ=60)
                                                                           AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                  >  SRR4302532.86934/1‑94 (MQ=60)
                                                                             GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                <  SRR4302532.1242112/94‑1 (MQ=60)
                                                                              AACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGC               >  SRR4302532.3431519/1‑94 (MQ=60)
                                                                               ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC              >  SRR4302532.1515796/1‑94 (MQ=60)
                                                                               ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC              >  SRR4302532.762767/1‑94 (MQ=60)
                                                                                 TTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCG            <  SRR4302532.3565793/94‑1 (MQ=60)
                                                                                 TTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACACGCCCG            >  SRR4302532.1011249/1‑94 (MQ=60)
                                                                                  TACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGG           <  SRR4302532.4884884/94‑1 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA         >  SRR4302532.155902/1‑94 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA         >  SRR4302532.155903/1‑94 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG       >  SRR4302532.543100/1‑94 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG       <  SRR4302532.1234191/94‑1 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG       <  SRR4302532.6285075/94‑1 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG       <  SRR4302532.1854956/94‑1 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA      >  SRR4302532.929730/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA      >  SRR4302532.1386115/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA      >  SRR4302532.1386116/1‑94 (MQ=60)
                                                                                        GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA     <  SRR4302532.3736131/94‑1 (MQ=60)
                                                                                         CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC    >  SRR4302532.2779699/1‑94 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTACCCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA   <  SRR4302532.45099/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA   <  SRR4302532.2183933/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA   <  SRR4302532.2183934/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA   <  SRR4302532.3780220/94‑1 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTAGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACACGCCCAGCACGTACAG  >  SRR4302532.169784/1‑94 (MQ=60)
                                                                                             |                                                                                           
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG  >  NC_000913/4103426‑4103609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑