Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,131,917 A→G N168S (AAC→AGC)  katG → catalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,131,9170AG96.1% 846.1 / 14.9 257N168S (AAC→AGC) katGcatalase
Reads supporting (aligned to +/- strand):  ref base A (7/3);  new base G (126/121);  total (133/124)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 261 total aligned reads displayed.

GCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGG  >  NC_000913/4131827‑4132006
                                                                                          |                                                                                         
gCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCg                                                                                         >  2:3392224/1‑93 (MQ=255)
gCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCg                                                                                         >  1:205879/1‑93 (MQ=255)
 ccTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGt                                                                                        <  1:3843478/93‑1 (MQ=255)
  cTCGATAAAGCGCGTCTCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTg                                                                                       <  2:6076923/93‑1 (MQ=255)
  cTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTg                                                                                       >  2:1187020/1‑93 (MQ=255)
   tCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTgg                                                                                      >  2:3300291/1‑93 (MQ=255)
   tCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTgg                                                                                      >  1:1288785/1‑93 (MQ=255)
    cGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgc                                                                                     <  1:3595774/93‑1 (MQ=255)
      aTAAAGCTCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgcgc                                                                                   <  1:4444716/93‑1 (MQ=255)
      aTAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgcgc                                                                                   <  1:4098828/93‑1 (MQ=255)
      aTAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGgcgc                                                                                   <  1:1218433/93‑1 (MQ=255)
       tAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCt                                                                                  >  1:1255726/1‑93 (MQ=255)
        aaaGCGCGTCGCCTGTTGTTGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTa                                                                                 <  2:4350451/93‑1 (MQ=255)
        aaaGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTa                                                                                 >  1:2311083/1‑93 (MQ=255)
         aaGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAg                                                                                >  2:5731726/1‑93 (MQ=255)
          aGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGa                                                                               >  2:2104545/1‑93 (MQ=255)
             gcgTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGaaaa                                                                            <  1:3042486/93‑1 (MQ=255)
             gcgTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGaaaa                                                                            <  1:3684635/93‑1 (MQ=255)
                tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTc                                                                         <  2:4678797/93‑1 (MQ=255)
                tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTc                                                                         >  1:5324519/1‑93 (MQ=255)
                gcGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTc                                                                         <  1:4524805/92‑1 (MQ=255)
                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       <  1:526512/93‑1 (MQ=255)
                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       >  2:150842/1‑93 (MQ=255)
                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       >  1:2949362/1‑93 (MQ=255)
                  gCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCg                                                                       >  2:3594945/1‑93 (MQ=255)
                   ccTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCgg                                                                      >  2:5208635/1‑93 (MQ=255)
                     tgttgtGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCt                                                                    >  2:5304290/1‑93 (MQ=255)
                      gttgtGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCtt                                                                   >  1:6085733/1‑93 (MQ=255)
                      gttgtGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCtt                                                                   >  1:5931226/1‑93 (MQ=255)
                         gtgGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCg                                                                >  1:4995361/1‑93 (MQ=255)
                            gCCAATCAAACAGAAATATGGTCAAAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTAc                                                             <  2:3645448/93‑1 (MQ=255)
                               aaTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCtt                                                          >  1:2580921/1‑93 (MQ=255)
                               aaTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCtt                                                          <  1:97835/93‑1 (MQ=255)
                                ataaaaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTc                                                         <  2:2184154/90‑1 (MQ=255)
                                aTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTc                                                         >  2:1252724/1‑93 (MQ=255)
                                aTCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTc                                                         <  1:5561578/93‑1 (MQ=255)
                                 tCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCg                                                        <  2:1374785/93‑1 (MQ=255)
                                 tCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCg                                                        >  2:1130909/1‑93 (MQ=255)
                                    aaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtt                                                     >  2:5107978/1‑93 (MQ=255)
                                     aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGttt                                                    <  1:2742198/93‑1 (MQ=255)
                                     aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGttt                                                    >  2:2918154/1‑93 (MQ=255)
                                     aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGttt                                                    <  2:2445305/93‑1 (MQ=255)
                                      cAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtttt                                                   >  2:3343906/1‑93 (MQ=255)
                                      cAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtttt                                                   >  1:1817552/1‑93 (MQ=255)
                                      cAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGtttt                                                   >  2:1537939/1‑93 (MQ=255)
                                       aGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTg                                                  >  2:2732103/1‑93 (MQ=255)
                                       aGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTg                                                  >  2:2685056/1‑93 (MQ=255)
                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTgg                                                 <  1:5237030/93‑1 (MQ=255)
                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTgg                                                 <  2:5172167/93‑1 (MQ=255)
                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTgg                                                 <  1:4331610/93‑1 (MQ=255)
                                           atatGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGc                                              >  1:4817838/1‑93 (MQ=255)
                                            tatGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGcc                                             <  2:2378736/93‑1 (MQ=255)
                                             atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCg                                            >  2:1328826/1‑93 (MQ=255)
                                             atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCg                                            <  1:5742824/93‑1 (MQ=255)
                                              tGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCgg                                           >  1:737371/1‑93 (MQ=255)
                                              tGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCgg                                           >  1:1935037/1‑93 (MQ=255)
                                                gTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTc                                         >  2:5364616/1‑93 (MQ=255)
                                                 tCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCg                                        <  1:5602037/93‑1 (MQ=255)
                                                   aGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTg                                      <  2:400955/93‑1 (MQ=255)
                                                    gAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGa                                     >  2:2552371/1‑93 (MQ=255)
                                                     aaaaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGaa                                    <  1:3229994/93‑1 (MQ=255)
                                                       aaTCTCCTGGGGTGACCTGTTTATCCGCGCGGGTAGCGTGGGGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  <  1:81018/93‑1 (MQ=255)
                                                       aaTCTCCTGGGCCGCCGTGTTTATCCTCGCGTGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  <  1:4717725/93‑1 (MQ=255)
                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  >  2:5859522/1‑93 (MQ=255)
                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  >  1:5842693/1‑93 (MQ=255)
                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  <  1:4772216/93‑1 (MQ=255)
                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGa                                  >  1:2552879/1‑93 (MQ=255)
                                                        aTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAc                                 <  2:1392507/93‑1 (MQ=255)
                                                        aTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAc                                 <  1:172176/93‑1 (MQ=255)
                                                          ctcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGt                               <  2:1990455/93‑1 (MQ=255)
                                                          ctcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGt                               >  1:2132481/1‑93 (MQ=255)
                                                           tcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTc                              <  1:1531470/93‑1 (MQ=255)
                                                           tcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTc                              <  2:305273/93‑1 (MQ=255)
                                                           tcCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTc                              >  2:738180/1‑93 (MQ=255)
                                                            ccTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCt                             <  1:3277679/93‑1 (MQ=255)
                                                             cTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAGGACGTCTg                            >  2:3955474/1‑93 (MQ=255)
                                                             cTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTg                            >  1:1765728/1‑93 (MQ=255)
                                                              tGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTgg                           <  2:2625904/93‑1 (MQ=255)
                                                               gggCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTggg                          >  2:919875/1‑93 (MQ=255)
                                                                  ccGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAGCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAAc                       <  2:4875005/93‑1 (MQ=255)
                                                                    gACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCg                     <  1:1017449/93‑1 (MQ=255)
                                                                     aCCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCgg                    >  2:2299388/1‑93 (MQ=255)
                                                                     aCCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCgg                    >  2:4588091/1‑93 (MQ=255)
                                                                      ccTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGa                   <  2:1915981/93‑1 (MQ=255)
                                                                        tGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATc                 <  1:5070314/93‑1 (MQ=255)
                                                                        tGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATc                 >  2:5540690/1‑93 (MQ=255)
                                                                        cgTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATc                 <  1:5877000/92‑1 (MQ=255)
                                                                          tttATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTg               <  1:2028148/93‑1 (MQ=255)
                                                                          tttATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTg               <  2:5393285/93‑1 (MQ=255)
                                                                          tttATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTg               <  2:5939842/93‑1 (MQ=255)
                                                                             aTCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGAt            <  2:2215903/93‑1 (MQ=255)
                                                                             aTCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGAt            >  1:4092228/1‑93 (MQ=255)
                                                                              tCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATg           >  1:4971845/1‑93 (MQ=255)
                                                                                cTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGtt         >  2:2515535/1‑93 (MQ=255)
                                                                                cTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGtt         >  2:648581/1‑93 (MQ=255)
                                                                                  cgcgGGTAGCGTGGCGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTaa       <  1:5408706/93‑1 (MQ=255)
                                                                                   gcgGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTAGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGACGTGGATGGTaat      >  1:3309915/1‑92 (MQ=255)
                                                                                     gggTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTg    <  2:1775520/93‑1 (MQ=255)
                                                                                      ggTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTgg   >  2:1861639/1‑93 (MQ=255)
                                                                                       gTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTggg  >  1:1618235/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGG  >  NC_000913/4131827‑4132006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTG  >  NC_000913/4131824‑4132009
                                                                                             |                                                                                            
TAAGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAG                                                                                              >  SRR4302532.3152720/1‑94 (MQ=60)
TAAGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAG                                                                                              <  SRR4302532.5569158/94‑1 (MQ=60)
 AAGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGC                                                                                             <  SRR4302532.199426/94‑1 (MQ=60)
  AGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCG                                                                                            >  SRR4302532.212913/1‑94 (MQ=60)
  AGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCG                                                                                            <  SRR4302532.3453739/94‑1 (MQ=60)
    CCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTG                                                                                          <  SRR4302532.3973249/94‑1 (MQ=60)
    CCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTG                                                                                          <  SRR4302532.5277499/94‑1 (MQ=60)
    CCTCGATAAAGCGCGTCGCCTGTTGGGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTG                                                                                          >  SRR4302532.6119605/1‑94 (MQ=60)
     CTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGG                                                                                         >  SRR4302532.737062/1‑94 (MQ=60)
     CTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGG                                                                                         >  SRR4302532.543530/1‑94 (MQ=60)
     CTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGG                                                                                         <  SRR4302532.6112964/94‑1 (MQ=60)
     CTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGG                                                                                         >  SRR4302532.1331906/1‑94 (MQ=60)
      TCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGC                                                                                        <  SRR4302532.453272/94‑1 (MQ=60)
       CGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCG                                                                                       <  SRR4302532.3716016/94‑1 (MQ=60)
        GATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGC                                                                                      >  SRR4302532.1429331/1‑94 (MQ=60)
         ATAAAGCTCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCT                                                                                     <  SRR4302532.4596194/94‑1 (MQ=60)
         ATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCT                                                                                     <  SRR4302532.4236418/94‑1 (MQ=60)
         ATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCT                                                                                     <  SRR4302532.1259207/94‑1 (MQ=60)
         ATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCT                                                                                     >  SRR4302532.1297731/1‑94 (MQ=60)
         ATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCT                                                                                     <  SRR4302532.3415918/94‑1 (MQ=60)
          TAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTA                                                                                    >  SRR4302532.2387985/1‑94 (MQ=60)
           AAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAG                                                                                   >  SRR4302532.5696264/1‑94 (MQ=60)
           AAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAG                                                                                   <  SRR4302532.5181943/94‑1 (MQ=60)
             AGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAA                                                                                 >  SRR4302532.2767641/1‑94 (MQ=60)
               CGCGTCGCCTGTTGTGGACAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAA                                                                               >  SRR4302532.5182924/1‑94 (MQ=60)
                GCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAAC                                                                              <  SRR4302532.3808373/94‑1 (MQ=60)
                GCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAAC                                                                              <  SRR4302532.2594626/94‑1 (MQ=60)
                GCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAAC                                                                              <  SRR4302532.3142883/94‑1 (MQ=60)
                  GTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTC                                                                            >  SRR4302532.5509039/1‑94 (MQ=60)
                   TCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCC                                                                           <  SRR4302532.1784581/94‑1 (MQ=60)
                   TCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCC                                                                           <  SRR4302532.132653/94‑1 (MQ=60)
                   TCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCC                                                                           <  SRR4302532.5928779/94‑1 (MQ=60)
                   GCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCC                                                                           <  SRR4302532.4679254/94‑1 (MQ=60)
                    CGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCG                                                                          <  SRR4302532.2363861/94‑1 (MQ=60)
                    CGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCG                                                                          >  SRR4302532.3047174/1‑94 (MQ=60)
                    CGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCG                                                                          <  SRR4302532.4738680/94‑1 (MQ=60)
                     GCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGG                                                                         <  SRR4302532.4584455/94‑1 (MQ=60)
                     GCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGG                                                                         <  SRR4302532.544883/94‑1 (MQ=60)
                      CCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGC                                                                        <  SRR4302532.1011918/94‑1 (MQ=60)
                        TGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTT                                                                      >  SRR4302532.6136544/1‑94 (MQ=60)
                        TGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTT                                                                      >  SRR4302532.6295865/1‑94 (MQ=60)
                          TTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCC                                                                    >  SRR4302532.1492442/1‑94 (MQ=60)
                           TGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCG                                                                   <  SRR4302532.3835667/94‑1 (MQ=60)
                           TGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCG                                                                   >  SRR4302532.5166588/1‑94 (MQ=60)
                            GTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGT                                                                  <  SRR4302532.2082239/94‑1 (MQ=60)
                             TGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTC                                                                 >  SRR4302532.764365/1‑94 (MQ=60)
                             TGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTA                                                                 <  SRR4302532.550295/94‑1 (MQ=60)
                             TGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTA                                                                 <  SRR4302532.778946/94‑1 (MQ=60)
                              GGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTTGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCggtgc                                                                >  SRR4302532.4983293/1‑89 (MQ=60)
                              GGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTAC                                                                >  SRR4302532.939255/1‑94 (MQ=60)
                                CCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCT                                                              >  SRR4302532.6036852/1‑94 (MQ=60)
                                 CAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTT                                                             <  SRR4302532.5160433/94‑1 (MQ=60)
                                 CAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTT                                                             >  SRR4302532.2667167/1‑94 (MQ=60)
                                 CAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTT                                                             >  SRR4302532.1882725/1‑94 (MQ=60)
                                  AATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTC                                                            <  SRR4302532.101234/94‑1 (MQ=60)
                                   ATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCG                                                           <  SRR4302532.5754680/94‑1 (MQ=60)
                                      AAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTT                                                        >  SRR4302532.2648673/1‑94 (MQ=60)
                                       AACAGAAATATGGTCAGAAAGGCTCCTGGGCCGGGCTGTTAGTCCTCGCGGGTAGGCTGGCGATAGAAAGCTCGGGCTTCCGTACCTTCGGTTT                                                       <  SRR4302532.3857147/94‑1 (MQ=23)
                                        ACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTT                                                      >  SRR4302532.1878136/1‑94 (MQ=60)
                                        ACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTT                                                      <  SRR4302532.2834318/94‑1 (MQ=60)
                                        ACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTT                                                      >  SRR4302532.1749308/1‑94 (MQ=60)
                                         CAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTG                                                     >  SRR4302532.5563552/1‑94 (MQ=60)
                                         CAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTG                                                     >  SRR4302532.4660332/1‑94 (MQ=60)
                                          AGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGG                                                    >  SRR4302532.3373191/1‑94 (MQ=60)
                                          AGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGG                                                    >  SRR4302532.57919/1‑94 (MQ=60)
                                          AGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGG                                                    <  SRR4302532.5020717/94‑1 (MQ=60)
                                           GAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGT                                                   <  SRR4302532.5417611/94‑1 (MQ=60)
                                           GAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGT                                                   <  SRR4302532.4477944/94‑1 (MQ=60)
                                            AAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTG                                                  >  SRR4302532.4220826/1‑94 (MQ=60)
                                            AAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTG                                                  <  SRR4302532.3247256/94‑1 (MQ=60)
                                             AATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGC                                                 >  SRR4302532.4983717/1‑94 (MQ=60)
                                             AATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGC                                                 >  SRR4302532.2496902/1‑94 (MQ=60)
                                              ATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCC                                                <  SRR4302532.3257185/94‑1 (MQ=60)
                                                ATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGG                                              >  SRR4302532.762484/1‑94 (MQ=60)
                                                ATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGG                                              <  SRR4302532.5942751/94‑1 (MQ=60)
                                                ATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGG                                              >  SRR4302532.1999434/1‑94 (MQ=60)
                                                  GGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTC                                            <  SRR4302532.1824891/94‑1 (MQ=60)
                                                  GGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTC                                            <  SRR4302532.2169136/94‑1 (MQ=60)
                                                   GTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCG                                           >  SRR4302532.6058219/1‑94 (MQ=60)
                                                    TCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGT                                          <  SRR4302532.5796490/94‑1 (MQ=60)
                                                      AGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGA                                        >  SRR4302532.128835/1‑94 (MQ=60)
                                                      AGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGA                                        >  SRR4302532.3792653/1‑94 (MQ=60)
                                                       GAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAA                                       >  SRR4302532.1838746/1‑94 (MQ=60)
                                                       GAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAA                                       <  SRR4302532.3590738/94‑1 (MQ=60)
                                                        AAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAG                                      <  SRR4302532.5427054/94‑1 (MQ=60)
                                                        AAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAG                                      <  SRR4302532.3337209/94‑1 (MQ=60)
                                                        AAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAG                                      <  SRR4302532.447565/94‑1 (MQ=60)
                                                         AAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGA                                     >  SRR4302532.6045472/1‑94 (MQ=60)
                                                         AAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGA                                     <  SRR4302532.6229273/94‑1 (MQ=60)
                                                         AAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGA                                     >  SRR4302532.2638015/1‑94 (MQ=60)
                                                          AATCTCCTGGGGTGACCTGTTTATCCGCGCGGGTAGCGTGGGGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAC                                    <  SRR4302532.83888/94‑1 (MQ=60)
                                                          AATCTCCTGGGCCGCCGTGTTTATCCTCGCGTGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAC                                    <  SRR4302532.4878691/94‑1 (MQ=60)
                                                          AATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAC                                    <  SRR4302532.4878692/94‑1 (MQ=60)
                                                          AATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAC                                    <  SRR4302532.4936410/94‑1 (MQ=60)
                                                          AATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGAC                                    <  SRR4302532.3067188/94‑1 (MQ=60)
                                                           ATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACG                                   <  SRR4302532.177941/94‑1 (MQ=60)
                                                           ATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACG                                   <  SRR4302532.2188510/94‑1 (MQ=60)
                                                           ATCTCCTCGGGCGACCTGTTTATCCTCGCGGGCAGCGTGGCGCTAGAAAACTCCGGATTCCGGACCTTCGGTTTTCGCGCCGGTCGTtcagagg                                   >  SRR4302532.150481/1‑87 (MQ=52)
                                                            TCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGT                                  >  SRR4302532.2203699/1‑94 (MQ=60)
                                                             CTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTC                                 >  SRR4302532.5490680/1‑94 (MQ=60)
                                                             CTCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTC                                 <  SRR4302532.4969222/94‑1 (MQ=60)
                                                              TCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCT                                <  SRR4302532.1583213/94‑1 (MQ=60)
                                                              TCCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCT                                <  SRR4302532.2852094/94‑1 (MQ=60)
                                                               CCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTG                               <  SRR4302532.3386693/94‑1 (MQ=60)
                                                               CCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTG                               <  SRR4302532.2053000/94‑1 (MQ=60)
                                                               CCTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTG                               >  SRR4302532.1824904/1‑94 (MQ=60)
                                                                CTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGG                              <  SRR4302532.4232891/94‑1 (MQ=60)
                                                                CTGGGCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGG                              >  SRR4302532.374209/1‑94 (MQ=60)
                                                                    GCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAAC                          <  SRR4302532.6278034/94‑1 (MQ=60)
                                                                    GCCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAAC                          <  SRR4302532.3386664/94‑1 (MQ=60)
                                                                     CCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACC                         <  SRR4302532.196236/94‑1 (MQ=60)
                                                                     CCGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACC                         <  SRR4302532.2983898/94‑1 (MQ=60)
                                                                      CGACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAAGCG                        >  SRR4302532.1227049/1‑94 (MQ=60)
                                                                       GACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGGTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGG                       >  SRR4302532.5000995/1‑94 (MQ=60)
                                                                       GACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGG                       >  SRR4302532.5980290/1‑94 (MQ=60)
                                                                       GACCTGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGG                       <  SRR4302532.1051981/94‑1 (MQ=60)
                                                                           TGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCT                   >  SRR4302532.412321/1‑94 (MQ=60)
                                                                           TGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCT                   <  SRR4302532.5244569/94‑1 (MQ=60)
                                                                           CGTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCT                   <  SRR4302532.6080594/94‑1 (MQ=60)
                                                                            GTTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTG                  >  SRR4302532.571975/1‑94 (MQ=60)
                                                                             TTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGG                 >  SRR4302532.2656698/1‑94 (MQ=60)
                                                                             TTTATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGG                 <  SRR4302532.2095741/94‑1 (MQ=60)
                                                                               TATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGAT               <  SRR4302532.47737/94‑1 (MQ=60)
                                                                               TATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGAT               >  SRR4302532.4229606/1‑94 (MQ=60)
                                                                                ATCCTCGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATG              >  SRR4302532.5142289/1‑94 (MQ=60)
                                                                                 TCCTCGCGGGTAGCGTGGCGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGT             <  SRR4302532.1622342/94‑1 (MQ=60)
                                                                                     CGCGGGTAGCGTGGCGCTAGAAACCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAAC         <  SRR4302532.5596106/94‑1 (MQ=60)
                                                                                     CGCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTAGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGAcgtggatggtaat         >  SRR4302532.3420241/1‑81 (MQ=60)
                                                                                      GCGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACT        <  SRR4302532.5761560/94‑1 (MQ=60)
                                                                                       GGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTG       <  SRR4302532.2109918/94‑1 (MQ=60)
                                                                                       CGGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTG       <  SRR4302532.1281974/94‑1 (MQ=60)
                                                                                        GGGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGG      <  SRR4302532.1607777/94‑1 (MQ=60)
                                                                                        GGGTAGCGGGGCGCTAGTAAACTCCGGATTCCGTACCTTCGGTTTTGGTGTCGGTCGTGCAGGCGTCTGGGtacccggtctggcaggtcacctg      >  SRR4302532.3780294/1‑71 (MQ=36)
                                                                                         GGTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGG     >  SRR4302532.1672994/1‑94 (MQ=60)
                                                                                         GGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGG     >  SRR4302532.3499286/1‑94 (MQ=60)
                                                                                          GTAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGG    <  SRR4302532.4315574/94‑1 (MQ=60)
                                                                                           TAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGT   >  SRR4302532.5474444/1‑94 (MQ=60)
                                                                                           TAGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGT   <  SRR4302532.1659235/94‑1 (MQ=60)
                                                                                            AGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTG  >  SRR4302532.219805/1‑94 (MQ=60)
                                                                                            AGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTG  <  SRR4302532.4073773/94‑1 (MQ=60)
                                                                                            AGCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTG  <  SRR4302532.2318590/94‑1 (MQ=60)
                                                                                             |                                                                                            
TAAGCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTG  >  NC_000913/4131824‑4132009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑