Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,949	+G	intergenic (+82/‑76)	nusG → / → rplK	NusG/50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,947	1	.	G	99.4%	635.8 / ‑3.9	178	intergenic (+80/‑78)	nusG/rplK	NusG/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (76/101); total (76/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 196 total aligned reads displayed.

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA  >  NC_000913/4175855‑4176034

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                                                                                          <  2:3914708/93‑1 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑g                                                                                        >  1:54674/1‑93 (MQ=255)

  aaaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gg                                                                                       <  1:3208820/93‑1 (MQ=255)

   aaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑ggg                                                                                      >  2:2141397/1‑92 (MQ=255)

    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                     >  2:1041787/1‑91 (MQ=255)

    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                     >  2:6058600/1‑91 (MQ=255)

     aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcc                                                                                    <  2:4264280/93‑4 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                <  2:1261800/93‑1 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                >  1:4653848/1‑93 (MQ=255)

           aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGccc                                                                               >  1:477381/1‑93 (MQ=255)

                aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa                                                                          <  1:1742751/93‑1 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaaa                                                                         <  2:2559999/93‑1 (MQ=255)

                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg                                                                       >  2:376253/1‑93 (MQ=255)

                    aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa                                                                      >  1:5227916/1‑93 (MQ=255)

                    aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa                                                                      >  2:1724033/1‑93 (MQ=255)

                     tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt                                                                     >  1:725529/1‑93 (MQ=255)

                     tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt                                                                     >  2:4770484/1‑93 (MQ=255)

                     tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt                                                                     >  1:2330482/1‑93 (MQ=255)

                     acAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt                                                                     <  1:4526766/92‑1 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt                                                                    >  2:5983123/1‑93 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtt                                                                    <  2:2098465/93‑1 (MQ=255)

                       aaaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAttt                                                                   >  2:1784173/1‑93 (MQ=255)

                        aaaaaGCGGCGATTTAATCTTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt                                                                  <  2:1743515/93‑1 (MQ=255)

                        aaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt                                                                  >  2:801075/1‑93 (MQ=255)

                        aaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt                                                                  <  2:3837453/93‑1 (MQ=255)

                          aaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttttt                                                                <  1:385065/93‑1 (MQ=255)

                          aaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttttt                                                                <  1:5107843/93‑1 (MQ=255)

                          aaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttttt                                                                >  2:2817481/1‑93 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTta                                                               <  1:2777463/93‑1 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTta                                                               <  2:5068848/93‑1 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTta                                                               >  1:2039409/1‑93 (MQ=255)

                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtat                                                              <  1:5930588/93‑1 (MQ=255)

                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtat                                                              <  1:829786/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtata                                                             <  1:2048527/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtata                                                             <  1:4362923/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGCGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtata                                                             >  1:587911/1‑93 (MQ=255)

                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTatat                                                            <  2:2475493/93‑1 (MQ=255)

                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTatat                                                            <  2:1926939/93‑1 (MQ=255)

                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTatat                                                            <  2:5752984/93‑1 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCa                                                          >  2:1093091/1‑93 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCa                                                          >  2:2637243/1‑93 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCa                                                          <  1:1149258/93‑1 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       >  2:4709479/1‑93 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       <  1:3779450/93‑1 (MQ=255)

                                     ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgggg                                                     >  1:4180713/1‑93 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                  >  1:626398/1‑93 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                  >  2:2278291/1‑93 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                  >  1:3511743/1‑93 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                 <  2:2741334/93‑1 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                 >  2:4851163/1‑93 (MQ=255)

                                         tCGTTGCAAAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                 >  1:3022913/1‑93 (MQ=255)

                                           gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctc                                               >  1:3459667/1‑93 (MQ=255)

                                           gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctc                                               >  1:4393133/1‑93 (MQ=255)

                                             tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc                                             <  2:3210826/93‑1 (MQ=255)

                                              gcgcaAAGCGTGAGATTGGAATGCAATTTCGCGTCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCa                                            <  1:5376898/90‑1 (MQ=255)

                                               cacaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCag                                           <  1:3156101/93‑1 (MQ=255)

                                               cacaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCag                                           >  1:2569319/1‑93 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAgggg                                        <  1:5553009/93‑1 (MQ=255)

                                                   aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGc                                       <  2:5118814/93‑1 (MQ=255)

                                                     gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGt                                     <  2:4420499/93‑1 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGTTTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGtta                                   <  1:1761968/93‑1 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                   <  2:3840831/93‑1 (MQ=255)

                                                        tGAGATTCGAATACAATTTCGCGCTTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGttat                                  <  1:1969613/93‑1 (MQ=255)

                                                         gagaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt                                 <  2:3987343/93‑1 (MQ=255)

                                                          agaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatta                                <  2:6116990/93‑1 (MQ=255)

                                                             ttGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTAccc                             >  2:3260746/1‑93 (MQ=255)

                                                             ttGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTAccc                             <  2:895940/93‑1 (MQ=255)

                                                             ttGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAccc                             <  1:5722502/93‑1 (MQ=255)

                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCa                            <  2:1411845/93‑1 (MQ=255)

                                                               ggAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCaa                           <  2:3241700/93‑1 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAAc                          <  2:5091116/93‑1 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTCCCCAAc                          <  1:2112295/93‑1 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                          >  2:3442135/1‑93 (MQ=255)

                                                                 aaTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                         <  2:935675/93‑1 (MQ=255)

                                                                  aTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACtt                        >  1:2389143/1‑93 (MQ=255)

                                                                  aTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACtt                        >  1:600989/1‑93 (MQ=255)

                                                                   tACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTg                       >  2:2519891/1‑93 (MQ=255)

                                                                   tACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTg                       >  2:4553439/1‑93 (MQ=255)

                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAg                     <  1:995278/93‑1 (MQ=255)

                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAg                     <  2:2116681/93‑1 (MQ=255)

                                                                      aaTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAgg                    <  2:3789274/93‑1 (MQ=255)

                                                                       aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGa                   >  2:335175/1‑93 (MQ=255)

                                                                        tttCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGaa                  <  2:3003774/93‑1 (MQ=255)

                                                                          tCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAAtt                <  1:2921090/93‑1 (MQ=255)

                                                                           cgcgCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAttt               <  1:683861/93‑1 (MQ=255)

                                                                            gcgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTta              >  2:4948556/1‑93 (MQ=255)

                                                                             cgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTtat             <  1:168435/93‑1 (MQ=255)

                                                                             cgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat             >  2:5437505/1‑93 (MQ=255)

                                                                              gcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtata            >  1:3538177/1‑93 (MQ=255)

                                                                               ccTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATaa           <  1:1243780/93‑1 (MQ=255)

                                                                               ccTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATaa           <  2:5306920/93‑1 (MQ=255)

                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATg         <  2:2355536/93‑1 (MQ=255)

                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATg         >  1:1321967/1‑93 (MQ=255)

                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg         >  1:4984166/1‑93 (MQ=255)

                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg         >  2:1212512/1‑93 (MQ=255)

                                                                                  tttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGCAGCGTTATTACCCAACTTGAGGAATTTATAATgg        >  2:5936347/1‑93 (MQ=255)

                                                                                      tttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTaa    <  2:161927/93‑1 (MQ=255)

                                                                                       ttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAg   <  1:40343/93‑1 (MQ=255)

                                                                                       ttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAg   >  2:1412770/1‑93 (MQ=255)

                                                                                        tttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGa  >  1:5397511/1‑93 (MQ=255)

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA  >  NC_000913/4175855‑4176034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175856‑4176042

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                                <  SRR4302532.972307/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                                <  SRR4302532.748526/94‑1 (MQ=60)

 AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑G                                                                                              <  SRR4302532.3315368/94‑1 (MQ=60)

  AAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑GC                                                                                             <  SRR4302532.4227255/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                           <  SRR4302532.586642/94‑1 (MQ=60)

      gtattccaatctcacgccttgtGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTG                                                                                          >  SRR4302532.5158797/23‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                        >  SRR4302532.4812509/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                        >  SRR4302532.2298025/1‑94 (MQ=60)

         TAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCC                                                                                       >  SRR4302532.494031/1‑94 (MQ=60)

              CAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAA                                                                                  <  SRR4302532.3919472/94‑1 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                 <  SRR4302532.1801391/94‑1 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                 >  SRR4302532.442632/1‑94 (MQ=60)

                GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAAC                                                                                <  SRR4302532.703792/94‑1 (MQ=60)

                GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAAC                                                                                <  SRR4302532.1725091/94‑1 (MQ=60)

                 CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACG                                                                               <  SRR4302532.2423057/94‑1 (MQ=60)

                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGA                                                                              >  SRR4302532.5408218/1‑94 (MQ=60)

                   ATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGAT                                                                             >  SRR4302532.2408108/1‑94 (MQ=60)

                   ATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGAT                                                                             >  SRR4302532.750254/1‑94 (MQ=60)

                    ACAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATT                                                                            <  SRR4302532.4681276/94‑1 (MQ=60)

                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTT                                                                          <  SRR4302532.4886874/94‑1 (MQ=60)

                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                       <  SRR4302532.398541/94‑1 (MQ=60)

                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                       >  SRR4302532.2107347/1‑94 (MQ=60)

                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                       <  SRR4302532.5283442/94‑1 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302532.2870667/94‑1 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302532.535566/94‑1 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                     <  SRR4302532.5583397/94‑1 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                     <  SRR4302532.6135886/94‑1 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                     <  SRR4302532.858196/94‑1 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGCGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                     >  SRR4302532.608423/1‑94 (MQ=60)

                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATAT                                                                    <  SRR4302532.2116744/94‑1 (MQ=60)

                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATAT                                                                    <  SRR4302532.4510823/94‑1 (MQ=60)

                              GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCA                                                                  <  SRR4302532.3380867/94‑1 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTttttacg                                                                 >  SRR4302532.2554951/1‑87 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCAC                                                                 <  SRR4302532.1187620/94‑1 (MQ=60)

                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTTTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                                <  SRR4302532.5980447/94‑1 (MQ=60)

                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                                >  SRR4302532.797069/1‑94 (MQ=60)

                                 GATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGG                                                               >  SRR4302532.1894204/1‑94 (MQ=60)

                                  ATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGG                                                              <  SRR4302532.3906781/94‑1 (MQ=60)

                                   TTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGG                                                             >  SRR4302532.4320945/1‑94 (MQ=60)

                                     TAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAG                                                           <  SRR4302532.2577181/94‑1 (MQ=60)

                                      AATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGC                                                          >  SRR4302532.648279/1‑94 (MQ=60)

                                      AATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGC                                                          >  SRR4302532.3629354/1‑94 (MQ=60)

                                       ATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCC                                                         >  SRR4302532.1817484/1‑94 (MQ=60)

                                       ATCGTTGCAAAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCC                                                         >  SRR4302532.3122622/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302532.4542561/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302532.3575532/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCCTTGCCCGTAAAACGATTTTTTATATCCCGGGGAGCCTC                                                       <  SRR4302532.2781463/94‑1 (MQ=60)

                                            TGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCA                                                    <  SRR4302532.2108159/94‑1 (MQ=60)

                                             GCGCAAAGCGTGAGATTGGAATGCAATTTCGCGTCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   <  SRR4302532.5563157/94‑1 (MQ=60)

                                             GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   >  SRR4302532.2655089/1‑94 (MQ=60)

                                              CACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGG                                                  <  SRR4302532.4161717/94‑1 (MQ=60)

                                              CACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGG                                                  <  SRR4302532.3260858/94‑1 (MQ=60)

                                               ACAAGTCGGGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGTCCGTAAAACGATTTTTTATATCACGGCGAGCCTTTCAGGG                                                 >  SRR4302532.416651/1‑94 (MQ=60)

                                                 AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGC                                               >  SRR4302532.3294754/1‑94 (MQ=60)

                                                 AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGC                                               <  SRR4302532.5745779/94‑1 (MQ=60)

                                                  AGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCG                                              >  SRR4302532.3635842/1‑94 (MQ=60)

                                                    GCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTT                                            <  SRR4302532.610837/94‑1 (MQ=60)

                                                    GCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTT                                            <  SRR4302532.5283186/94‑1 (MQ=60)

                                                      GTGAGATTGGAATACAATTTCGCGTTTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTAT                                          <  SRR4302532.1821062/94‑1 (MQ=60)

                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTAT                                          <  SRR4302532.3096063/94‑1 (MQ=60)

                                                       TGAGATTCGAATACAATTTCGCGCTTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATT                                         <  SRR4302532.2035049/94‑1 (MQ=60)

                                                         AGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAC                                       >  SRR4302532.2919016/1‑94 (MQ=60)

                                                            TTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCA                                    <  SRR4302532.5921829/94‑1 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTGAAACCATTTTTTATATCACCGGGAGCCTCTCAGACGCGTTATTACCCAAC                                  >  SRR4302532.2488229/1‑94 (MQ=60)

                                                               GAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTCCCCAACT                                 <  SRR4302532.2182833/94‑1 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTT                                >  SRR4302532.2468736/1‑94 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                >  SRR4302532.621977/1‑94 (MQ=60)

                                                                 ATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTG                               >  SRR4302532.5734677/1‑94 (MQ=60)

                                                                 ATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTG                               <  SRR4302532.1754965/94‑1 (MQ=60)

                                                                 ATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTG                               >  SRR4302532.6064543/1‑94 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302532.471305/94‑1 (MQ=60)

                                                                    CAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGG                            <  SRR4302532.1029214/94‑1 (MQ=60)

                                                                       TTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAAT                         <  SRR4302532.2157253/94‑1 (MQ=60)

                                                                         TCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTT                       <  SRR4302532.3018358/94‑1 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTA                      >  SRR4302532.3677544/1‑94 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTA                      >  SRR4302532.4966783/1‑94 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                      <  SRR4302532.707347/94‑1 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCATGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTA                      <  SRR4302532.4627982/94‑1 (MQ=60)

                                                                            CGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATA                    <  SRR4302532.174076/94‑1 (MQ=60)

                                                                            CGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                    >  SRR4302532.3656554/1‑94 (MQ=60)

                                                                              CCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAAT                  <  SRR4302532.1285395/94‑1 (MQ=60)

                                                                              CCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  <  SRR4302532.621535/94‑1 (MQ=60)

                                                                               CTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATG                 >  SRR4302532.1366141/1‑94 (MQ=60)

                                                                               CTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                 <  SRR4302532.2055678/94‑1 (MQ=60)

                                                                               CTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                 >  SRR4302532.5155025/1‑94 (MQ=60)

                                                                                  TTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCT              <  SRR4302532.551750/94‑1 (MQ=60)

                                                                                   TGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTA             <  SRR4302532.1308986/94‑1 (MQ=60)

                                                                                     TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG           <  SRR4302532.6310055/94‑1 (MQ=60)

                                                                                      TTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA          >  SRR4302532.3222946/1‑94 (MQ=60)

                                                                                      TTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA          <  SRR4302532.41798/94‑1 (MQ=60)

                                                                                      TTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA          >  SRR4302532.5584530/1‑94 (MQ=60)

                                                                                      TTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACGTGAGGAATTTATAATGGCTAAGA          >  SRR4302532.3611743/1‑94 (MQ=60)

                                                                                       TTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAA         >  SRR4302532.2000137/1‑94 (MQ=60)

                                                                                         TATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG       >  SRR4302532.396430/1‑94 (MQ=60)

                                                                                         TATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG       <  SRR4302532.2542844/94‑1 (MQ=60)

                                                                                          atgGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGT      >  SRR4302532.2831507/4‑94 (MQ=60)

                                                                                           tgGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTA     >  SRR4302532.2570854/3‑94 (MQ=60)

                                                                                           G‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    <  SRR4302532.1026411/94‑1 (MQ=60)

                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302532.4468873/94‑1 (MQ=60)

                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302532.5393084/94‑1 (MQ=60)

                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302532.2072473/94‑1 (MQ=60)

                                                                                              G‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  <  SRR4302532.1078278/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175856‑4176042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`