Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,949	+G	intergenic (+82/‑76)	nusG → / → rplK	NusG/50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,947	1	.	G	98.7%	839.1 / 1.3	231	intergenic (+80/‑78)	nusG/rplK	NusG/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (102/126); total (103/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 269 total aligned reads displayed.

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG  >  NC_000913/4175855‑4176033

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑t                                                                                         <  1:433909/93‑1 (MQ=255)

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑t                                                                                         <  1:5977735/93‑1 (MQ=255)

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑t                                                                                         <  1:1830025/93‑1 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑g                                                                                       >  1:1500985/1‑93 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑g                                                                                       <  1:3253081/93‑1 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑g                                                                                       <  1:2392182/93‑1 (MQ=255)

  aaaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gg                                                                                      <  2:4156834/93‑1 (MQ=255)

   aaaaGCCTAACCCAGCGATCAAAAGAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑ggg                                                                                     >  1:6406168/1‑92 (MQ=255)

   aaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑ggg                                                                                     >  1:1901459/1‑92 (MQ=255)

    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gggc                                                                                    <  2:4060538/93‑3 (MQ=255)

    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gggc                                                                                    >  1:6335774/1‑91 (MQ=255)

     aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gggcc                                                                                   <  2:6054940/93‑4 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gggcct                                                                                  <  2:2746821/93‑5 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gggcct                                                                                  <  1:4765128/93‑5 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑gggcct                                                                                  <  2:321273/93‑5 (MQ=255)

        ccTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTg                                                                                 <  2:5515811/93‑1 (MQ=255)

         cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACGATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGc                                                                                >  2:1116671/1‑93 (MQ=255)

         cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGc                                                                                >  2:813996/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGcc                                                                               >  2:2339830/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGcc                                                                               <  1:2344613/93‑1 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGcc                                                                               <  2:3305947/93‑1 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGcc                                                                               >  1:6069815/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGcc                                                                               >  1:4214155/1‑93 (MQ=255)

           aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGccc                                                                              <  2:5180667/93‑1 (MQ=255)

           aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGccc                                                                              >  1:3744192/1‑93 (MQ=255)

            aCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCg                                                                             >  2:1248841/1‑93 (MQ=255)

              ccAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTa                                                                           >  1:5302975/1‑93 (MQ=255)

               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTaa                                                                          >  1:478392/1‑93 (MQ=255)

                aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTaaa                                                                         <  1:1106128/93‑1 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTaaaa                                                                        >  2:235844/1‑93 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTaaaa                                                                        >  2:3644576/1‑93 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTaaaa                                                                        >  1:5388070/1‑93 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTaaaa                                                                        >  1:2007414/1‑93 (MQ=255)

                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACg                                                                      >  2:584831/1‑93 (MQ=255)

                    aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGa                                                                     >  2:1983913/1‑93 (MQ=255)

                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTACGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGAtt                                                                   >  2:2914046/1‑93 (MQ=255)

                       aaaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGAttt                                                                  <  2:2243035/93‑1 (MQ=255)

                       aaaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGAttt                                                                  <  2:6626134/93‑1 (MQ=255)

                       aaaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGAttt                                                                  <  1:2682219/93‑1 (MQ=255)

                          aaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGAtttttt                                                               >  1:46531/1‑93 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTta                                                              >  1:1068044/1‑93 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTtata                                                            <  2:5129479/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTtata                                                            <  2:5371561/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTtata                                                            <  2:5739301/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTtaca                                                            >  1:3915947/1‑91 (MQ=255)

                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTatat                                                           <  2:5687671/93‑1 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                          >  2:4028881/1‑93 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                          >  1:2293649/1‑93 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCa                                                         <  2:887831/93‑1 (MQ=255)

                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCAc                                                        <  2:6586552/93‑1 (MQ=255)

                                  gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACg                                                       >  2:1527482/1‑93 (MQ=255)

                                  gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACg                                                       >  1:537143/1‑93 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                      <  2:523488/93‑1 (MQ=255)

                                    tttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACggg                                                     >  2:4531971/1‑93 (MQ=255)

                                     ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACgggg                                                    <  1:2618035/93‑1 (MQ=255)

                                      tAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGa                                                   <  2:1420950/93‑1 (MQ=255)

                                       aaTCGTTGCACAAGGCGCGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAg                                                  >  1:2203445/1‑93 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                 >  1:5853711/1‑93 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                <  2:345262/93‑1 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                <  2:1783894/93‑1 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                >  2:2529908/1‑93 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                               <  1:5028977/93‑1 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                               <  1:304716/93‑1 (MQ=255)

                                           gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctc                                              >  1:2974421/1‑93 (MQ=255)

                                            gtGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                             <  1:1170250/92‑1 (MQ=255)

                                             tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc                                            <  2:4814257/93‑1 (MQ=255)

                                             tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc                                            >  2:5988176/1‑93 (MQ=255)

                                             tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc                                            <  1:3563685/93‑1 (MQ=255)

                                              gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCa                                           <  1:5678057/93‑1 (MQ=255)

                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga                                         >  2:6297040/1‑93 (MQ=255)

                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga                                         >  2:4579991/1‑93 (MQ=255)

                                                acaAGGCCTGAGATTGGAATTCAATTTCGCGCTTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATCTCACGGGGAGCCTCTCaga                                         <  1:6416581/93‑1 (MQ=255)

                                                 caAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAGCGATTTTTTATATCACGGGGAGCCTCTCagag                                        >  1:5077756/1‑93 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTACG‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAgg                                       >  2:3546531/1‑93 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAgg                                       <  2:1092569/93‑1 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAgg                                       <  2:1278124/93‑1 (MQ=255)

                                                   aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                      >  2:5279617/1‑93 (MQ=255)

                                                      cGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtt                                   <  2:563203/93‑1 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                  >  1:5284359/1‑93 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                  <  1:4714820/93‑1 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                  >  1:1687794/1‑93 (MQ=255)

                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                           <  2:3757958/93‑1 (MQ=255)

                                                               ggAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCaa                          <  1:3601318/93‑1 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                         >  2:1881309/1‑93 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                         <  2:5404703/93‑1 (MQ=255)

                                                                  aTACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACtt                       >  2:6184858/1‑93 (MQ=255)

                                                                   tACAATTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTg                      >  2:3241776/1‑93 (MQ=255)

                                                                       aTTTCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                  <  1:5141175/93‑1 (MQ=255)

                                                                        tttCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTgaagaa                 <  1:461061/93‑1 (MQ=255)

                                                                        tttCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGaa                 >  1:79152/1‑93 (MQ=255)

                                                                         ttCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAAt                <  1:3440053/93‑1 (MQ=255)

                                                                         ttCGCGCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAt                <  2:6436409/93‑1 (MQ=255)

                                                                           cgcgCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGATGAAttt              >  2:500251/1‑93 (MQ=255)

                                                                           cgcgCCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAttt              <  2:4233655/93‑1 (MQ=255)

                                                                            gcgcCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta             >  1:626125/1‑93 (MQ=255)

                                                                              gcCTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtata           >  2:4958341/1‑93 (MQ=255)

                                                                               ccTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATaa          <  2:729258/93‑1 (MQ=255)

                                                                                cTTTTGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAt         >  1:2824951/1‑93 (MQ=255)

                                                                                 ttttGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg        >  1:154169/1‑93 (MQ=255)

                                                                                   ttGTTTTTA‑TGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGc      <  1:1576956/93‑1 (MQ=255)

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA‑T‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG  >  NC_000913/4175855‑4176033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA  >  NC_000913/4175856‑4176041

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               <  SRR4302547.3318077/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               <  SRR4302547.6723106/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               <  SRR4302547.2440644/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               <  SRR4302547.6290133/94‑1 (MQ=60)

 AAAAAGCCTAACCCAGCGATCAAAAGAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑G                                                                                             >  SRR4302547.6539766/1‑94 (MQ=60)

 AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑G                                                                                             >  SRR4302547.1939075/1‑94 (MQ=60)

  AAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑GC                                                                                            >  SRR4302547.6467695/1‑94 (MQ=60)

   AAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGgcc                                                                                            <  SRR4302547.3664253/94‑4 (MQ=60)

    AAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCT                                                                                           <  SRR4302547.2853918/94‑1 (MQ=60)

    AAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCT                                                                                           >  SRR4302547.5498436/1‑94 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                          >  SRR4302547.985560/1‑94 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                          >  SRR4302547.3510844/1‑94 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                          <  SRR4302547.2604117/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                          <  SRR4302547.4863840/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                          <  SRR4302547.5434468/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                          >  SRR4302547.814968/1‑94 (MQ=60)

      GCCTAACCGAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTG                                                                                         <  SRR4302547.2874306/94‑1 (MQ=60)

      GCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTG                                                                                         <  SRR4302547.2545321/94‑1 (MQ=60)

       CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGC                                                                                        >  SRR4302547.5318336/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                       >  SRR4302547.4300166/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                       >  SRR4302547.6195716/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                       >  SRR4302547.3217646/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                       <  SRR4302547.6496722/94‑1 (MQ=60)

         TAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCC                                                                                      >  SRR4302547.3820616/1‑94 (MQ=60)

         TAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCC                                                                                      <  SRR4302547.2392131/94‑1 (MQ=60)

          AACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCG                                                                                     >  SRR4302547.2619051/1‑94 (MQ=60)

          AACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCG                                                                                     <  SRR4302547.4759344/94‑1 (MQ=60)

           ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGT                                                                                    <  SRR4302547.1925067/94‑1 (MQ=60)

            CCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTA                                                                                   >  SRR4302547.5412362/1‑94 (MQ=60)

             CCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAA                                                                                  >  SRR4302547.487178/1‑94 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATATAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                <  SRR4302547.5396311/94‑1 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                >  SRR4302547.5499330/1‑94 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                >  SRR4302547.4722981/1‑94 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                <  SRR4302547.1128393/94‑1 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAA                                                                                >  SRR4302547.2047357/1‑94 (MQ=60)

                GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAAC                                                                               >  SRR4302547.3381256/1‑94 (MQ=60)

                GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAAC                                                                               <  SRR4302547.3899003/94‑1 (MQ=60)

                 CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACG                                                                              <  SRR4302547.3139154/94‑1 (MQ=60)

                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGA                                                                             >  SRR4302547.2419280/1‑94 (MQ=60)

                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGA                                                                             >  SRR4302547.1127102/1‑94 (MQ=60)

                    TCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATT                                                                           >  SRR4302547.2699099/1‑94 (MQ=60)

                     CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTGTGGG‑CCTTGCCCGTAAAACGATTT                                                                          >  SRR4302547.1617560/1‑94 (MQ=60)

                     CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTT                                                                          <  SRR4302547.546799/94‑1 (MQ=60)

                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTT                                                                         <  SRR4302547.2208914/94‑1 (MQ=60)

                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTT                                                                         <  SRR4302547.2736076/94‑1 (MQ=60)

                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTT                                                                         >  SRR4302547.6402379/1‑94 (MQ=60)

                       AAAAAGGGCCGATTTAATCGTTGCACAAGGCGGGAGATTGGAATACAATTTGGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTT                                                                        >  SRR4302547.89837/1‑94 (MQ=60)

                       AAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTT                                                                        <  SRR4302547.1339350/94‑1 (MQ=60)

                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                       >  SRR4302547.47398/1‑94 (MQ=60)

                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                       <  SRR4302547.6136137/94‑1 (MQ=60)

                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                       >  SRR4302547.2354743/1‑94 (MQ=60)

                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                      >  SRR4302547.1089307/1‑94 (MQ=60)

                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                      <  SRR4302547.1940730/94‑1 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                    >  SRR4302547.5000876/1‑94 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTACA                                                                    >  SRR4302547.3995567/1‑94 (MQ=60)

                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAACCGATTTTTTATAT                                                                   >  SRR4302547.2289724/1‑94 (MQ=60)

                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATAT                                                                   >  SRR4302547.4324860/1‑94 (MQ=60)

                             CGGCGATTTAATCGTTGCACAGGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATC                                                                  <  SRR4302547.1588307/94‑1 (MQ=60)

                             CGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATC                                                                  >  SRR4302547.2340108/1‑94 (MQ=60)

                             CGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATC                                                                  <  SRR4302547.1462394/94‑1 (MQ=60)

                              GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCA                                                                 >  SRR4302547.6450353/1‑94 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCAC                                                                <  SRR4302547.6545806/94‑1 (MQ=60)

                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                               >  SRR4302547.1157270/1‑94 (MQ=60)

                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                               >  SRR4302547.546843/1‑94 (MQ=60)

                                    TTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                           <  SRR4302547.2670783/94‑1 (MQ=60)

                                    TTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                           <  SRR4302547.3046927/94‑1 (MQ=60)

                                    TTAATCGTTGCAAAAGGCGTTAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                           <  SRR4302547.4118991/94‑1 (MQ=60)

                                     TAATCGTTGCACAAGGCGCGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAG                                                          >  SRR4302547.2247711/1‑94 (MQ=60)

                                      AATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGC                                                         >  SRR4302547.5974821/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                      <  SRR4302547.5132890/94‑1 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                      >  SRR4302547.4873894/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                      >  SRR4302547.3033612/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                      <  SRR4302547.310393/94‑1 (MQ=60)

                                          GTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCT                                                     <  SRR4302547.44400/94‑1 (MQ=60)

                                          GTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCT                                                     >  SRR4302547.5760364/1‑94 (MQ=60)

                                           TTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGTCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTC                                                    <  SRR4302547.1675236/94‑1 (MQ=60)

                                           GTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTC                                                    <  SRR4302547.1193909/94‑1 (MQ=60)

                                            TGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCA                                                   <  SRR4302547.3636397/94‑1 (MQ=60)

                                             GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                  <  SRR4302547.5795302/94‑1 (MQ=60)

                                             GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                  <  SRR4302547.1971406/94‑1 (MQ=60)

                                              CACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGA                                                 >  SRR4302547.5894787/1‑94 (MQ=60)

                                               ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAGCGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                >  SRR4302547.5182507/1‑94 (MQ=60)

                                               ACAAGGCCTGAGATTGGAATTCAATTTCGCGCTTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATCTCACGGGGAGCCTCTCAGAG                                                <  SRR4302547.6550376/94‑1 (MQ=60)

                                                 AAGGCGTGAGATTGGAATACAATTGCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAACCGATTTTTTATATCACGGGGAGCCTCTCAGGGGC                                              <  SRR4302547.3903600/94‑1 (MQ=60)

                                                   GGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGT                                            <  SRR4302547.1741837/94‑1 (MQ=60)

                                                   GGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGT                                            >  SRR4302547.6472525/1‑94 (MQ=60)

                                                    GCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTT                                           <  SRR4302547.5207241/94‑1 (MQ=60)

                                                    GCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTT                                           >  SRR4302547.356881/1‑94 (MQ=60)

                                                     CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                          >  SRR4302547.1721004/1‑94 (MQ=60)

                                                     CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                          <  SRR4302547.608236/94‑1 (MQ=60)

                                                     CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                          >  SRR4302547.5393359/1‑94 (MQ=60)

                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                         >  SRR4302547.4889163/1‑94 (MQ=60)

                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                         <  SRR4302547.4812430/94‑1 (MQ=60)

                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                         >  SRR4302547.3542052/1‑94 (MQ=60)

                                                           ATTGGAATACAATTTCGCGCTTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCC                                    <  SRR4302547.6767840/94‑1 (MQ=60)

                                                            TTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCA                                   <  SRR4302547.1171604/94‑1 (MQ=60)

                                                             TGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAA                                  >  SRR4302547.2384527/1‑94 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAAC                                 <  SRR4302547.3674757/94‑1 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                 <  SRR4302547.3645578/94‑1 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                 >  SRR4302547.5374686/1‑94 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                               <  SRR4302547.478886/94‑1 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                            <  SRR4302547.2898040/94‑1 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                            <  SRR4302547.4878666/94‑1 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                            <  SRR4302547.4265832/94‑1 (MQ=60)

                                                                    CAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGG                           <  SRR4302547.2298233/94‑1 (MQ=60)

                                                                      ATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAA                         >  SRR4302547.80632/1‑94 (MQ=60)

                                                                      ATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAA                         <  SRR4302547.5247079/94‑1 (MQ=60)

                                                                       TTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAAGAAT                        <  SRR4302547.469578/94‑1 (MQ=60)

                                                                        TTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATT                       <  SRR4302547.3510192/94‑1 (MQ=60)

                                                                        TTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATT                       >  SRR4302547.2732017/1‑94 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTA                     <  SRR4302547.6047453/94‑1 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                     >  SRR4302547.5928529/1‑94 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                     >  SRR4302547.637687/1‑94 (MQ=60)

                                                                           GCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTAT                    >  SRR4302547.304438/1‑94 (MQ=60)

                                                                           GCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTAT                    >  SRR4302547.3799327/1‑94 (MQ=60)

                                                                            CGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGGGGCGTTATTACCCAACTTGAGGAATTTATA                   <  SRR4302547.5142943/94‑1 (MQ=60)

                                                                            CGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                   >  SRR4302547.4827876/1‑94 (MQ=60)

                                                                              CCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                 <  SRR4302547.5215825/94‑1 (MQ=60)

                                                                              CCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                 >  SRR4302547.2881455/1‑94 (MQ=60)

                                                                               CTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                >  SRR4302547.156986/1‑94 (MQ=60)

                                                                               CTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                >  SRR4302547.1004588/1‑94 (MQ=60)

                                                                                TTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG               >  SRR4302547.160381/1‑94 (MQ=60)

                                                                                TTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG               <  SRR4302547.4709837/94‑1 (MQ=60)

                                                                                  TTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCT             <  SRR4302547.1608338/94‑1 (MQ=60)

                                                                                  TTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCT             <  SRR4302547.5487116/94‑1 (MQ=60)

                                                                                    GTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAA           <  SRR4302547.1925301/94‑1 (MQ=60)

                                                                                     TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG          <  SRR4302547.4592268/94‑1 (MQ=60)

                                                                                        TTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATaacgcctctgagaggct       <  SRR4302547.6715996/94‑18 (MQ=60)

                                                                                         TATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG      <  SRR4302547.1334480/94‑1 (MQ=60)

                                                                                         TATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG      <  SRR4302547.3257064/94‑1 (MQ=60)

                                                                                           tgGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTA    <  SRR4302547.4174103/92‑1 (MQ=60)

                                                                                           tgGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTA    >  SRR4302547.4253186/3‑94 (MQ=60)

                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA  <  SRR4302547.5861708/94‑1 (MQ=60)

                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA  >  SRR4302547.4548789/1‑94 (MQ=60)

                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA  >  SRR4302547.557810/1‑94 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA  >  NC_000913/4175856‑4176041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`