Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,177,426 T→C intergenic (+265/‑148) rplA → / → rplJ 50S ribosomal subunit protein L1/50S ribosomal subunit protein L10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,177,4260TC98.7% 788.3 / ‑2.4 223intergenic (+265/‑148)rplA/rplJ50S ribosomal subunit protein L1/50S ribosomal subunit protein L10
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (109/110);  total (111/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 228 total aligned reads displayed.

GAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTT  >  NC_000913/4177336‑4177515
                                                                                          |                                                                                         
gAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAAccc                                                                                         >  2:4473654/1‑93 (MQ=255)
 aaTAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAAcccc                                                                                        <  2:312750/93‑1 (MQ=255)
       gACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGt                                                                                  >  2:5371608/1‑93 (MQ=255)
          ggATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGa                                                                               <  1:577616/93‑1 (MQ=255)
          ggATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGa                                                                               >  2:3646414/1‑93 (MQ=255)
          ggATAAAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGa                                                                               >  1:5006191/1‑93 (MQ=255)
           gATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGAc                                                                              <  1:5723619/93‑1 (MQ=255)
            aTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACg                                                                             <  1:4143811/93‑1 (MQ=255)
            aTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACg                                                                             <  2:2956669/93‑1 (MQ=255)
             tAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACgg                                                                            >  1:5606050/1‑93 (MQ=255)
              aGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACggg                                                                           >  2:1512847/1‑92 (MQ=255)
              aGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGt                                                                           <  1:318238/93‑1 (MQ=255)
                 aaaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGAc                                                                        >  2:52873/1‑93 (MQ=255)
                 aaaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGAc                                                                        <  1:5268248/93‑1 (MQ=255)
                 aaaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGAc                                                                        >  2:2642224/1‑93 (MQ=255)
                  aaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACa                                                                       <  1:835040/93‑1 (MQ=255)
                  aaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACa                                                                       <  2:4296924/93‑1 (MQ=255)
                  aaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACa                                                                       >  2:2155716/1‑93 (MQ=255)
                  aaGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACa                                                                       >  1:1115691/1‑93 (MQ=255)
                    aaTTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGa                                                                     <  2:3489005/92‑1 (MQ=255)
                     aTTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGaa                                                                    <  2:1116887/93‑1 (MQ=255)
                      tttGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAAc                                                                   <  2:5652022/93‑1 (MQ=255)
                      tttGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAAc                                                                   >  1:3654646/1‑93 (MQ=255)
                        tgtgCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGc                                                                 <  2:85338/93‑1 (MQ=255)
                        tGTTCGTTGGAGCCTGGCTTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGc                                                                 <  1:294502/93‑1 (MQ=255)
                        tGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGc                                                                 >  2:260661/1‑93 (MQ=255)
                         gTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCt                                                                >  1:4604540/1‑93 (MQ=255)
                         gTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCt                                                                <  1:4571040/93‑1 (MQ=255)
                          ttCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTa                                                               <  1:715894/93‑1 (MQ=255)
                           tCGTTGGACCCGGGCCTATCCAGGCCTCCTTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTaa                                                              <  1:1908084/93‑1 (MQ=255)
                             gTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGa                                                            >  1:200710/1‑93 (MQ=255)
                             gTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGa                                                            >  2:3532086/1‑93 (MQ=255)
                              ttGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGat                                                           >  2:1334740/1‑93 (MQ=255)
                              ttGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGat                                                           >  2:19381/1‑93 (MQ=255)
                              ttGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGat                                                           >  1:318327/1‑93 (MQ=255)
                               tGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGatt                                                          <  1:1791487/93‑1 (MQ=255)
                                ggAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGatta                                                         <  2:4012572/93‑1 (MQ=255)
                                ggAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGGTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGatta                                                         >  1:1304605/1‑93 (MQ=255)
                                  aGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGattatt                                                       >  2:1312265/1‑93 (MQ=255)
                                  aGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGattatt                                                       <  2:719184/93‑1 (MQ=255)
                                  aGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGattatt                                                       <  2:2707785/93‑1 (MQ=255)
                                   gCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTc                                                      <  1:5437690/93‑1 (MQ=255)
                                    ccTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTCAGATTATTCt                                                     >  1:4524583/1‑93 (MQ=255)
                                    ccTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCt                                                     <  2:3549116/93‑1 (MQ=255)
                                     cTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCtt                                                    >  1:5743723/1‑93 (MQ=255)
                                     cTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCtt                                                    >  1:207882/1‑93 (MQ=255)
                                     cTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCtt                                                    >  2:390687/1‑93 (MQ=255)
                                      tGGCCTATCCAGGCCTCCGTCGAAGAACGCAGGAGATTCGCAAGAAACTTAACCACCTGCGTAGACGGTGACAGAACGCTAAGATTATTCttt                                                   >  1:6146611/1‑93 (MQ=255)
                                      cggCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCttt                                                   >  1:477841/2‑93 (MQ=255)
                                         ccTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTtat                                                <  1:3751323/93‑1 (MQ=255)
                                           tATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTatat                                              >  1:1253369/1‑93 (MQ=255)
                                           tATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTatat                                              <  2:2455169/93‑1 (MQ=255)
                                            aTCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAtt                                             >  1:5956902/1‑93 (MQ=255)
                                            aTCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAtt                                             <  2:5843410/93‑1 (MQ=255)
                                            aTCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAtt                                             <  2:1059772/93‑1 (MQ=255)
                                            aTCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAtt                                             <  1:279650/93‑1 (MQ=255)
                                            aTCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAtt                                             >  1:47328/1‑93 (MQ=255)
                                             tCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTc                                            >  1:1718925/1‑93 (MQ=255)
                                             tCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTc                                            <  1:281388/93‑1 (MQ=255)
                                             tCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTc                                            >  1:5448817/1‑93 (MQ=255)
                                                aGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTgg                                         >  2:1791300/1‑93 (MQ=255)
                                                aGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTgg                                         >  1:3476810/1‑93 (MQ=255)
                                                 ggCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGc                                        >  1:2015087/1‑93 (MQ=255)
                                                   ccTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCtt                                      <  1:5111977/93‑1 (MQ=255)
                                                   ccTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCtt                                      >  2:4600452/1‑93 (MQ=255)
                                                    cTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTg                                     >  2:4853536/1‑93 (MQ=255)
                                                    cTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTg                                     <  2:2198105/93‑1 (MQ=255)
                                                     tCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGt                                    <  1:3595890/93‑1 (MQ=255)
                                                      ccGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGtt                                   >  1:4785220/1‑93 (MQ=255)
                                                      ccGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGtt                                   <  2:1260186/93‑1 (MQ=255)
                                                      ccGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGtt                                   <  1:3936619/93‑1 (MQ=255)
                                                        gTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTc                                 <  1:4202282/93‑1 (MQ=255)
                                                        gTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTc                                 <  1:1641825/93‑1 (MQ=255)
                                                          cGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTg                               >  2:610168/1‑93 (MQ=255)
                                                           gAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGc                              >  2:108528/1‑93 (MQ=255)
                                                           gAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGc                              >  2:3576324/1‑93 (MQ=255)
                                                                  gCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGt                       >  2:1955026/1‑93 (MQ=255)
                                                                  gCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGt                       >  1:5420198/1‑93 (MQ=255)
                                                                     ggAGGTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCGTTTATATTCTGGCTTGTTTCTGCTCACCGTAAt                    >  1:3333898/1‑93 (MQ=255)
                                                                      gAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAAtt                   >  2:4367979/1‑93 (MQ=255)
                                                                        gTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACTGTAATTaa                 >  2:911600/1‑93 (MQ=255)
                                                                        gTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTaa                 >  1:2558247/1‑93 (MQ=255)
                                                                         tttCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAg                >  1:6047396/1‑93 (MQ=255)
                                                                         tttCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAg                <  1:1155656/93‑1 (MQ=255)
                                                                         tttCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAg                >  2:3092338/1‑93 (MQ=255)
                                                                          ttCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGa               <  2:3016093/93‑1 (MQ=255)
                                                                          ttCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGa               <  1:74679/93‑1 (MQ=255)
                                                                           tCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGAc              <  2:2092083/93‑1 (MQ=255)
                                                                            cGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACg             >  2:3243660/1‑93 (MQ=255)
                                                                            cGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACg             <  1:491566/93‑1 (MQ=255)
                                                                             gCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGc            >  2:2763963/1‑93 (MQ=255)
                                                                             gCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTACTCACCGTAATTAAGACGc            <  2:5966842/93‑1 (MQ=255)
                                                                                aGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGctct         >  1:4650064/1‑93 (MQ=255)
                                                                                 gAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGctctc        <  2:3856355/93‑1 (MQ=255)
                                                                                  aaaCTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGctctct       <  1:3433751/93‑1 (MQ=255)
                                                                                  aaaCTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGctctct       <  1:2394803/93‑1 (MQ=255)
                                                                                  aaaCTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGCAATTAAGACGctctct       >  1:943169/1‑93 (MQ=255)
                                                                                   aaCTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCtctctc      <  2:1734093/93‑1 (MQ=255)
                                                                                      ttAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGt   >  1:2136830/1‑93 (MQ=255)
                                                                                       tAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGtt  >  1:1564744/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTT  >  NC_000913/4177336‑4177515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTTTG  >  NC_000913/4177334‑4177517
                                                                                            |                                                                                           
CTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACC                                                                                            >  SRR4302532.1792457/1‑94 (MQ=60)
CTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACC                                                                                            >  SRR4302532.3808116/1‑94 (MQ=60)
   AATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCC                                                                                         >  SRR4302532.5648/1‑94 (MQ=60)
   AATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAAATTAACCCCC                                                                                         >  SRR4302532.346321/1‑94 (MQ=60)
     TAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTG                                                                                       <  SRR4302532.5901891/94‑1 (MQ=60)
      AGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGC                                                                                      >  SRR4302532.995564/1‑94 (MQ=60)
       GCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCG                                                                                     <  SRR4302532.4983257/94‑1 (MQ=60)
       GCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCG                                                                                     <  SRR4302532.4865300/94‑1 (MQ=60)
         GACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTA                                                                                   >  SRR4302532.6208747/1‑94 (MQ=60)
           CGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAAACCCCTGCGTAGA                                                                                 >  SRR4302532.1328238/1‑94 (MQ=60)
           CGGATAAAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGA                                                                                 >  SRR4302532.5177767/1‑94 (MQ=60)
            GGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGAC                                                                                <  SRR4302532.597751/94‑1 (MQ=60)
            GGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCAAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGGAGAC                                                                                <  SRR4302532.597752/94‑1 (MQ=60)
             GATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACG                                                                               <  SRR4302532.5922969/94‑1 (MQ=60)
             GATAGAAAAAATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACG                                                                               <  SRR4302532.5674552/94‑1 (MQ=60)
              ATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGG                                                                              <  SRR4302532.4282828/94‑1 (MQ=60)
              ATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGG                                                                              >  SRR4302532.5153269/1‑94 (MQ=60)
              ATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGG                                                                              >  SRR4302532.5800639/1‑94 (MQ=60)
              ATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGG                                                                              >  SRR4302532.388688/1‑94 (MQ=60)
              ATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGG                                                                              <  SRR4302532.5937160/94‑1 (MQ=60)
                AGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTG                                                                            <  SRR4302532.329275/94‑1 (MQ=60)
                AGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTG                                                                            <  SRR4302532.329274/94‑1 (MQ=60)
                 GAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGA                                                                           <  SRR4302532.1817281/94‑1 (MQ=60)
                 GAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGA                                                                           >  SRR4302532.6344177/1‑94 (MQ=60)
                  AAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGAC                                                                          <  SRR4302532.6064707/94‑1 (MQ=60)
                  AAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGAC                                                                          >  SRR4302532.2690601/1‑94 (MQ=60)
                   AAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACA                                                                         >  SRR4302532.1152969/1‑94 (MQ=60)
                   AAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACA                                                                         <  SRR4302532.5449915/94‑1 (MQ=60)
                    AAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAG                                                                        <  SRR4302532.5972070/94‑1 (MQ=60)
                    AAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAG                                                                        <  SRR4302532.863679/94‑1 (MQ=60)
                     AGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGA                                                                       >  SRR4302532.3772384/1‑94 (MQ=60)
                     AGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGA                                                                       <  SRR4302532.3403290/94‑1 (MQ=60)
                     AGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGA                                                                       <  SRR4302532.3176912/94‑1 (MQ=60)
                      GATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAA                                                                      >  SRR4302532.700116/1‑94 (MQ=60)
                       ATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAAC                                                                     >  SRR4302532.3777338/1‑94 (MQ=60)
                       ATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAAC                                                                     >  SRR4302532.1199411/1‑94 (MQ=60)
                        TTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACG                                                                    <  SRR4302532.2084416/94‑1 (MQ=60)
                          TGTTCGTTGGAGCCTGGCTTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCT                                                                  <  SRR4302532.304658/94‑1 (MQ=60)
                          TGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCT                                                                  >  SRR4302532.4761542/1‑94 (MQ=60)
                           GTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTA                                                                 <  SRR4302532.4726946/94‑1 (MQ=60)
                           GTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTA                                                                 >  SRR4302532.3110251/1‑94 (MQ=60)
                            TTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAA                                                                <  SRR4302532.740350/94‑1 (MQ=60)
                            TTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAA                                                                >  SRR4302532.1162109/1‑94 (MQ=60)
                             TCGTTGGACCCGGGCCTATCCAGGCCTCCTTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAG                                                               <  SRR4302532.1971404/94‑1 (MQ=60)
                              CGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGA                                                              >  SRR4302532.207564/1‑94 (MQ=60)
                               GTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGAT                                                             >  SRR4302532.329368/1‑94 (MQ=60)
                                TTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATT                                                            <  SRR4302532.4525617/94‑1 (MQ=60)
                                 TGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTA                                                           <  SRR4302532.1851341/94‑1 (MQ=60)
                                 TGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTA                                                           <  SRR4302532.2814682/94‑1 (MQ=60)
                                 TGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGGTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTA                                                           >  SRR4302532.1348272/1‑94 (MQ=60)
                                  GGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTAT                                                          <  SRR4302532.3558670/94‑1 (MQ=60)
                                  GGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTAT                                                          <  SRR4302532.4966064/94‑1 (MQ=60)
                                     GCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTCAGATTATTCT                                                       >  SRR4302532.4679030/1‑94 (MQ=60)
                                     GCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCT                                                       >  SRR4302532.902130/1‑94 (MQ=60)
                                     GCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCT                                                       <  SRR4302532.5626137/94‑1 (MQ=60)
                                      CCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTT                                                      >  SRR4302532.5943674/1‑94 (MQ=60)
                                      CCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTT                                                      >  SRR4302532.214980/1‑94 (MQ=60)
                                      CCTGGCCTAACAAGGCCTCAGTCGAAGACCGCAAGAGTTACACAAGAAACTTAACACCCTGCGTAGACGGTGACAGAACGataaaaatcttatt                                                      <  SRR4302532.5649277/94‑15 (MQ=39)
                                       CTGGCCTATCCAGGCCTCCGTCGAAGAACGCAGGAGATTCGCAAGAAACTTAACCACCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTT                                                     >  SRR4302532.6358611/1‑94 (MQ=60)
                                       CCGGCCTATCCCGGCCTCCGTCGAAGACCGCAGGAGATTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTT                                                     >  SRR4302532.494505/1‑94 (MQ=60)
                                       CCGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTT                                                     >  SRR4302532.494504/1‑94 (MQ=60)
                                         GGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTA                                                   >  SRR4302532.3876801/1‑94 (MQ=60)
                                          GCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTAT                                                  <  SRR4302532.4789922/94‑1 (MQ=60)
                                           CCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATA                                                 <  SRR4302532.3877444/94‑1 (MQ=60)
                                            CTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAT                                                >  SRR4302532.1881475/1‑94 (MQ=60)
                                            CTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATAT                                                >  SRR4302532.1295318/1‑94 (MQ=60)
                                             TATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATT                                               >  SRR4302532.5635513/1‑94 (MQ=60)
                                             TATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATT                                               >  SRR4302532.48999/1‑94 (MQ=60)
                                             TATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATT                                               >  SRR4302532.6162948/1‑94 (MQ=60)
                                              ATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTT                                              >  SRR4302532.1703235/1‑94 (MQ=60)
                                              ATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTC                                              >  SRR4302532.1776842/1‑94 (MQ=60)
                                              ATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTC                                              >  SRR4302532.736967/1‑94 (MQ=60)
                                              ATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTC                                              >  SRR4302532.5637670/1‑94 (MQ=60)
                                              ATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTC                                              <  SRR4302532.1979940/94‑1 (MQ=60)
                                              ATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTC                                              <  SRR4302532.289339/94‑1 (MQ=60)
                                               TCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCT                                             <  SRR4302532.291117/94‑1 (MQ=60)
                                                 CAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGG                                           >  SRR4302532.3593270/1‑94 (MQ=60)
                                                 CAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGG                                           <  SRR4302532.5656857/94‑1 (MQ=60)
                                                  AGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGC                                          >  SRR4302532.2082274/1‑94 (MQ=60)
                                                  AGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGC                                          >  SRR4302532.2082276/1‑94 (MQ=60)
                                                   GGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCT                                         <  SRR4302532.358039/94‑1 (MQ=60)
                                                     CCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTG                                       <  SRR4302532.5287749/94‑1 (MQ=60)
                                                      CTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGT                                      >  SRR4302532.74568/1‑94 (MQ=60)
                                                      CTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGT                                      >  SRR4302532.429131/1‑94 (MQ=60)
                                                       TCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTT                                     >  SRR4302532.4949997/1‑94 (MQ=60)
                                                       TCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTT                                     <  SRR4302532.3716136/94‑1 (MQ=60)
                                                        CCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTT                                    <  SRR4302532.1522743/94‑1 (MQ=60)
                                                        CCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTT                                    <  SRR4302532.4069261/94‑1 (MQ=60)
                                                         CGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTC                                   >  SRR4302532.5481723/1‑94 (MQ=60)
                                                          GTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCT                                  <  SRR4302532.2248826/94‑1 (MQ=60)
                                                          GTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCT                                  <  SRR4302532.4343361/94‑1 (MQ=60)
                                                          GTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCT                                  <  SRR4302532.1697431/94‑1 (MQ=60)
                                                          GTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCT                                  <  SRR4302532.1656075/94‑1 (MQ=60)
                                                                GACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCAC                            <  SRR4302532.4748113/94‑1 (MQ=60)
                                                                 ACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACC                           <  SRR4302532.1866367/94‑1 (MQ=60)
                                                                 ACCGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACC                           >  SRR4302532.4915989/1‑94 (MQ=60)
                                                                   CGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGT                         >  SRR4302532.2141290/1‑94 (MQ=60)
                                                                   CGCAGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGT                         >  SRR4302532.5608059/1‑94 (MQ=60)
                                                                     CAGGAGTTTCGCAAGAAACTTAACCCCCTGCGGAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGCCTTGTTTCTGCTCACCGTTA                       >  SRR4302532.2632808/1‑94 (MQ=60)
                                                                      AGGAGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAAT                      <  SRR4302532.4133509/94‑1 (MQ=60)
                                                                      AGGAGGTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCGTTTATATTCTGGCTTGTTTCTGCTCACCGTAAT                      >  SRR4302532.3445100/1‑94 (MQ=60)
                                                                         AGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAA                   >  SRR4302532.2643586/1‑94 (MQ=60)
                                                                         AGTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAA                   >  SRR4302532.4686819/1‑94 (MQ=60)
                                                                          GTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAG                  >  SRR4302532.6256212/1‑94 (MQ=60)
                                                                          GTTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAG                  <  SRR4302532.4490142/94‑1 (MQ=60)
                                                                           TTTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGA                 <  SRR4302532.1194214/94‑1 (MQ=60)
                                                                           TTTCGCAAGAAAATTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGATTGTTTCTGCTCACCGTAATTAAGA                 <  SRR4302532.5241408/94‑1 (MQ=60)
                                                                            TTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGAC                <  SRR4302532.2901175/94‑1 (MQ=60)
                                                                            TTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGAC                <  SRR4302532.77352/94‑1 (MQ=60)
                                                                            TTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGAC                <  SRR4302532.3624412/94‑1 (MQ=60)
                                                                            TTCGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGAC                <  SRR4302532.1457328/94‑1 (MQ=60)
                                                                              CGCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGC              <  SRR4302532.508745/94‑1 (MQ=60)
                                                                               GCAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCT             >  SRR4302532.3004989/1‑94 (MQ=60)
                                                                                CAAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTATGCTCACCGTAATTAAGACGCTC            >  SRR4302532.6037753/1‑94 (MQ=60)
                                                                                 AAGAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCT           >  SRR4302532.4808610/1‑94 (MQ=60)
                                                                                   GAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCT         <  SRR4302532.4892606/94‑1 (MQ=60)
                                                                                   GAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCT         <  SRR4302532.781743/94‑1 (MQ=60)
                                                                                   GAAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGCAATTAAGACGCTCTCT         >  SRR4302532.975469/1‑94 (MQ=60)
                                                                                    AAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTC        <  SRR4302532.2474565/94‑1 (MQ=60)
                                                                                    AAACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTC        <  SRR4302532.3548806/94‑1 (MQ=60)
                                                                                     AACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCC       <  SRR4302532.3834902/94‑1 (MQ=60)
                                                                                     AACTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCC       <  SRR4302532.5500391/94‑1 (MQ=60)
                                                                                       CTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGT     >  SRR4302532.2547888/1‑94 (MQ=60)
                                                                                       CTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGT     >  SRR4302532.2208197/1‑94 (MQ=60)
                                                                                       CTTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTCGCTTGTTTCTGCTTACCGTAATTAAGACGCTCTCTCCGT     >  SRR4302532.2208196/1‑94 (MQ=60)
                                                                                        TTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTT    >  SRR4302532.1617679/1‑94 (MQ=60)
                                                                                        TTAACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTT    <  SRR4302532.537820/94‑1 (MQ=60)
                                                                                          AACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTTTG  <  SRR4302532.3683283/94‑1 (MQ=60)
                                                                                          AACCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTTTG  <  SRR4302532.5775599/94‑1 (MQ=60)
                                                                                            |                                                                                           
CTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCTGCTCACCGTAATTAAGACGCTCTCTCCGTTTG  >  NC_000913/4177334‑4177517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑