Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,216,491 T→C V106A (GTG→GCG)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,216,4910TC98.6% 755.6 / ‑1.6 218V106A (GTG→GCG) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (105/110);  total (105/113)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 227 total aligned reads displayed.

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC  >  NC_000913/4216401‑4216580
                                                                                          |                                                                                         
cgGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCgcgt                                                                                         >  2:4429203/1‑93 (MQ=255)
 ggCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTa                                                                                        >  2:3304852/1‑93 (MQ=255)
 ggCAGTTTTACTACGTGTTAAAGAGCATTGCACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTa                                                                                        <  1:189742/93‑1 (MQ=255)
  gCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTAc                                                                                       <  2:1137607/93‑1 (MQ=255)
   cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACt                                                                                      <  2:2680340/93‑1 (MQ=255)
   cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACt                                                                                      <  2:5423741/93‑1 (MQ=255)
     ttttttCTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGt                                                                                    <  1:1628494/93‑1 (MQ=255)
     tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGt                                                                                    <  1:2894301/93‑1 (MQ=255)
     tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGt                                                                                    <  2:472774/93‑1 (MQ=255)
          actacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGtt                                                                               >  2:2060056/1‑93 (MQ=255)
            tacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAt                                                                             >  2:3988903/1‑93 (MQ=255)
            tacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAt                                                                             >  1:4272891/1‑93 (MQ=255)
            tacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAt                                                                             >  1:2398245/1‑93 (MQ=255)
             acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAtt                                                                            <  1:4062357/93‑1 (MQ=255)
                tgtTAAAGAGCATTACACCCTGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGa                                                                         >  1:5931982/1‑93 (MQ=255)
                tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGa                                                                         >  1:675325/1‑93 (MQ=255)
                  ttAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGacc                                                                       >  1:171279/1‑93 (MQ=255)
                    aaaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccac                                                                     <  2:2779796/93‑1 (MQ=255)
                     aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccacc                                                                    <  1:3424362/93‑1 (MQ=255)
                     aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccacc                                                                    <  1:3353631/93‑1 (MQ=255)
                     aaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccacc                                                                    >  2:4298483/1‑93 (MQ=255)
                      agagCATTACACCCGGCTGTTGCCGGATTTCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcg                                                                   <  2:3461216/93‑1 (MQ=255)
                      agagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcg                                                                   >  2:4920980/1‑93 (MQ=255)
                       gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgc                                                                  >  2:3751517/1‑93 (MQ=255)
                       gagCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgc                                                                  <  1:2021480/93‑1 (MQ=255)
                        agCATTACCCCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgct                                                                 <  1:5827954/93‑1 (MQ=255)
                        agCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgct                                                                 <  2:6036646/93‑1 (MQ=255)
                         gCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGCTATTTGACCACcgctc                                                                >  1:868228/1‑93 (MQ=255)
                            ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgctcgct                                                             <  2:2572225/93‑1 (MQ=255)
                            ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgctcgct                                                             >  2:5531764/1‑93 (MQ=255)
                            ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgctcgct                                                             >  1:3528580/1‑93 (MQ=255)
                             tACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCtt                                                            <  1:5632916/93‑1 (MQ=255)
                              acacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTa                                                           <  1:2767450/93‑1 (MQ=255)
                               cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTAc                                                          <  2:2673960/93‑1 (MQ=255)
                                acCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACt                                                         <  2:1033370/93‑1 (MQ=255)
                                acCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACt                                                         <  1:4835755/93‑1 (MQ=255)
                                 cccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTc                                                        >  2:1290041/1‑93 (MQ=255)
                                  ccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTcc                                                       <  2:1690674/93‑1 (MQ=255)
                                  ccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTcc                                                       <  1:1216372/93‑1 (MQ=255)
                                   cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                      <  2:6110712/93‑1 (MQ=255)
                                   cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                      <  1:4817908/93‑1 (MQ=255)
                                   cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                      >  1:3900175/1‑93 (MQ=255)
                                   cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                      >  1:424847/1‑93 (MQ=255)
                                   cGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTccc                                                      >  1:4265179/1‑93 (MQ=255)
                                     gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                    <  1:4626029/93‑1 (MQ=255)
                                     gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                    >  2:5371855/1‑93 (MQ=255)
                                     gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                    >  2:391446/1‑93 (MQ=255)
                                      cTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAg                                                   <  1:167325/93‑1 (MQ=255)
                                       tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                  >  2:4788566/1‑93 (MQ=255)
                                       tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                  >  2:1916643/1‑93 (MQ=255)
                                       tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                  >  2:5791566/1‑93 (MQ=255)
                                       tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                  >  2:2250910/1‑93 (MQ=255)
                                        gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                 >  1:4556852/1‑93 (MQ=255)
                                        gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                 >  2:1593015/1‑93 (MQ=255)
                                         ttGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCgg                                                >  1:5219300/1‑93 (MQ=255)
                                           gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCt                                              >  1:1192610/1‑93 (MQ=255)
                                           gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGCTATTTGACCACCGCTCGCTTACTCCCGAGcatat                                              >  2:3164439/1‑89 (MQ=255)
                                            ccGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtt                                             >  1:6046226/1‑93 (MQ=255)
                                             cGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCttt                                            >  2:4636577/1‑93 (MQ=255)
                                                aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtttttt                                         >  2:4111276/1‑92 (MQ=255)
                                                  tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                       <  1:4500753/93‑1 (MQ=255)
                                                    cccTCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                     <  1:2148248/93‑1 (MQ=255)
                                                    cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                     <  1:2684228/93‑1 (MQ=255)
                                                      cgcgCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTa                                   >  1:6071814/1‑93 (MQ=255)
                                                      cgcgCTGCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTa                                   <  2:195036/93‑1 (MQ=255)
                                                       gcgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAg                                  <  1:2241201/93‑1 (MQ=255)
                                                        cgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGc                                 <  2:738857/93‑1 (MQ=255)
                                                         gcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGct                                >  1:5728774/1‑93 (MQ=255)
                                                          cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                               <  1:4428822/93‑1 (MQ=255)
                                                          cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctc                               >  2:3264642/1‑93 (MQ=255)
                                                           ttCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                              <  2:4389861/93‑1 (MQ=255)
                                                           ttCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                              <  2:1331343/93‑1 (MQ=255)
                                                             cGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCa                            >  1:2844349/1‑93 (MQ=255)
                                                             cGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCa                            <  1:1608346/93‑1 (MQ=255)
                                                              gagaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCac                           >  2:5369020/1‑92 (MQ=255)
                                                               agaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGc                          <  2:247386/93‑1 (MQ=255)
                                                                gaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                         >  1:2208045/1‑93 (MQ=255)
                                                                gaTTGCGGAGAGCTTCTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                         >  1:4521135/1‑93 (MQ=255)
                                                                 aTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                        <  2:15243/93‑1 (MQ=255)
                                                                 aTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                        <  2:5649077/93‑1 (MQ=255)
                                                                      ggAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcg                   >  2:5930104/1‑93 (MQ=255)
                                                                       gagaGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTGCTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                  <  2:1566462/93‑1 (MQ=255)
                                                                       gagaGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgcgc                  <  1:4783968/93‑1 (MQ=255)
                                                                        agagCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgcc                 >  1:1757842/1‑93 (MQ=255)
                                                                        agagCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgcc                 <  2:2896685/93‑1 (MQ=255)
                                                                         gagCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                >  1:5890604/1‑93 (MQ=255)
                                                                          agCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccgc               >  1:744423/1‑93 (MQ=255)
                                                                          agCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccgc               <  1:3286908/93‑1 (MQ=255)
                                                                             ttttttAACTCCGTGTGCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt            <  2:2270546/93‑1 (MQ=255)
                                                                             ttttttAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt            >  1:5401892/1‑93 (MQ=255)
                                                                             ttttttAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCttt            >  2:1280109/1‑93 (MQ=255)
                                                                              tttttAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTc           <  2:4892322/93‑1 (MQ=255)
                                                                                 ttAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTa        <  1:1101589/93‑1 (MQ=255)
                                                                                   aaCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc      >  2:4969494/1‑93 (MQ=255)
                                                                                    aCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa     <  2:4530447/93‑1 (MQ=255)
                                                                                    aCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa     >  1:1063300/1‑93 (MQ=255)
                                                                                     cTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAt    >  1:315092/1‑93 (MQ=255)
                                                                                      tCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGTGGCCTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGCACCAtt   >  1:5485365/1‑93 (MQ=255)
                                                                                      tCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt   >  1:630422/1‑93 (MQ=255)
                                                                                       ccGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTc  <  2:5856778/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC  >  NC_000913/4216401‑4216580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  >  NC_000913/4216399‑4216583
                                                                                            |                                                                                            
CGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCG                                                                                             <  SRR4302532.3331862/94‑1 (MQ=60)
CGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCG                                                                                             <  SRR4302532.5410199/94‑1 (MQ=60)
 GCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGT                                                                                            >  SRR4302532.2036767/1‑94 (MQ=60)
  CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTA                                                                                           >  SRR4302532.880187/1‑94 (MQ=60)
   GGCAGTTTTACTACGTGTTAAAGAGCATTGCACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTAC                                                                                          <  SRR4302532.196131/94‑1 (MQ=60)
     CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTG                                                                                        <  SRR4302532.1129574/94‑1 (MQ=60)
     CATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTG                                                                                        <  SRR4302532.6303240/94‑1 (MQ=60)
      ATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGT                                                                                       <  SRR4302532.4601419/94‑1 (MQ=60)
      ATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGT                                                                                       <  SRR4302532.587821/94‑1 (MQ=60)
       TTTTTTCTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTC                                                                                      <  SRR4302532.1683620/94‑1 (MQ=60)
       TTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTC                                                                                      <  SRR4302532.2990948/94‑1 (MQ=60)
        TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCG                                                                                     >  SRR4302532.6225389/1‑94 (MQ=60)
         TTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGG                                                                                    >  SRR4302532.1427902/1‑94 (MQ=60)
           TACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTT                                                                                  <  SRR4302532.2701964/94‑1 (MQ=60)
             CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                >  SRR4302532.4416754/1‑94 (MQ=60)
             CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                >  SRR4302532.2478101/1‑94 (MQ=60)
             CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                >  SRR4302532.4978809/1‑94 (MQ=60)
             CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTGTAACTCCGCGTACTGTCGGTTAT                                                                                >  SRR4302532.3620437/1‑94 (MQ=60)
              TACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATT                                                                               <  SRR4302532.4404204/94‑1 (MQ=60)
               ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTT                                                                              <  SRR4302532.4175128/94‑1 (MQ=60)
               ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTT                                                                              <  SRR4302532.4198879/94‑1 (MQ=60)
                CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTG                                                                             <  SRR4302532.4262430/94‑1 (MQ=60)
                CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTG                                                                             <  SRR4302532.1807392/94‑1 (MQ=60)
                CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTG                                                                             <  SRR4302532.4414374/94‑1 (MQ=60)
                 GTGTTAAAGAGCATTACACCCTGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGA                                                                            >  SRR4302532.6137320/1‑94 (MQ=60)
                 GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGA                                                                            <  SRR4302532.4795994/94‑1 (MQ=60)
                 GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGA                                                                            >  SRR4302532.698561/1‑94 (MQ=60)
                  TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGAC                                                                           >  SRR4302532.2222801/1‑94 (MQ=60)
                   GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                          >  SRR4302532.177017/1‑94 (MQ=60)
                   GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                          <  SRR4302532.5718371/94‑1 (MQ=60)
                       AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCG                                                                      <  SRR4302532.3539087/94‑1 (MQ=60)
                       AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCG                                                                      <  SRR4302532.3465591/94‑1 (MQ=60)
                       AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCG                                                                      <  SRR4302532.2955759/94‑1 (MQ=60)
                         GAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCT                                                                    <  SRR4302532.2088869/94‑1 (MQ=60)
                         GAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCT                                                                    <  SRR4302532.167530/94‑1 (MQ=60)
                          AGCATTACCCCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTC                                                                   <  SRR4302532.6030310/94‑1 (MQ=60)
                          AGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTC                                                                   <  SRR4302532.6011279/94‑1 (MQ=60)
                          AGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGCTATTTGACCACCGCTC                                                                   >  SRR4302532.897983/1‑94 (MQ=60)
                           GCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCG                                                                  <  SRR4302532.1189945/94‑1 (MQ=60)
                            CATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGC                                                                 >  SRR4302532.702720/1‑94 (MQ=60)
                             ATTACGCCCGGCTGTCGCCGCATTGCCCGCGCTCCGATATGGCGGAGAGCTTTTTTACCTCCGCGTCCTGTCGGTTATTTGACCACCGCTCTCT                                                                <  SRR4302532.6339796/94‑1 (MQ=36)
                             ATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCT                                                                >  SRR4302532.3646689/1‑94 (MQ=60)
                             ATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCT                                                                >  SRR4302532.3478865/1‑94 (MQ=60)
                               TACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTA                                                              <  SRR4302532.5828545/94‑1 (MQ=60)
                                ACACCCGGCTGTTGCCGGATTGCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTAC                                                             <  SRR4302532.6135333/94‑1 (MQ=60)
                                ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTAC                                                             <  SRR4302532.2860370/94‑1 (MQ=60)
                                  ACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTC                                                           >  SRR4302532.2581672/1‑94 (MQ=60)
                                  ACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTC                                                           <  SRR4302532.5002202/94‑1 (MQ=60)
                                    CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                         >  SRR4302532.4408737/1‑94 (MQ=60)
                                    CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                         <  SRR4302532.1257087/94‑1 (MQ=60)
                                    CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                         >  SRR4302532.439564/1‑94 (MQ=60)
                                    CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                         <  SRR4302532.2859005/94‑1 (MQ=60)
                                    CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                         >  SRR4302532.2312325/1‑94 (MQ=60)
                                    CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                         >  SRR4302532.4031709/1‑94 (MQ=60)
                                     CGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCG                                                        <  SRR4302532.4983790/94‑1 (MQ=60)
                                       GCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAG                                                      <  SRR4302532.4783754/94‑1 (MQ=60)
                                        CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                     <  SRR4302532.172933/94‑1 (MQ=60)
                                        CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                     >  SRR4302532.3966894/1‑94 (MQ=60)
                                         TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                    <  SRR4302532.6058527/94‑1 (MQ=60)
                                         TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                    >  SRR4302532.4712313/1‑94 (MQ=60)
                                          GTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGG                                                   >  SRR4302532.5399300/1‑94 (MQ=60)
                                            TGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCT                                                 >  SRR4302532.1232487/1‑94 (MQ=60)
                                             GCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTT                                                <  SRR4302532.3599183/94‑1 (MQ=60)
                                             GCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTT                                                >  SRR4302532.6254990/1‑94 (MQ=60)
                                             GCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTT                                                >  SRR4302532.6254989/1‑94 (MQ=60)
                                               CGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTT                                              <  SRR4302532.4528934/94‑1 (MQ=60)
                                                GGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTT                                             >  SRR4302532.4813805/1‑94 (MQ=60)
                                                 GATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTT                                            >  SRR4302532.4646952/1‑94 (MQ=60)
                                                  ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAT                                           >  SRR4302532.5921847/1‑94 (MQ=60)
                                                    TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                         <  SRR4302532.4266487/94‑1 (MQ=60)
                                                    TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                         <  SRR4302532.4654412/94‑1 (MQ=60)
                                                    TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTCACTCCCGAGCGGCTTTTTATCT                                         <  SRR4302532.6246664/94‑1 (MQ=60)
                                                      CCCTCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                       <  SRR4302532.2220040/94‑1 (MQ=60)
                                                      CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                       <  SRR4302532.2774361/94‑1 (MQ=60)
                                                       CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                      <  SRR4302532.4698848/94‑1 (MQ=60)
                                                       CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                      >  SRR4302532.6281513/1‑94 (MQ=60)
                                                       CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                      <  SRR4302532.1482674/94‑1 (MQ=60)
                                                       CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTA                                      <  SRR4302532.1732358/94‑1 (MQ=60)
                                                         GCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGC                                    <  SRR4302532.2315577/94‑1 (MQ=60)
                                                          CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                   >  SRR4302532.5928289/1‑94 (MQ=60)
                                                            CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                 <  SRR4302532.4579703/94‑1 (MQ=60)
                                                             TTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTC                                >  SRR4302532.1693588/1‑94 (MQ=60)
                                                             TTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTC                                <  SRR4302532.668650/94‑1 (MQ=60)
                                                              TCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCCCCGCTCGGTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCA                               >  SRR4302532.3398328/1‑94 (MQ=60)
                                                              TCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCA                               >  SRR4302532.2939423/1‑94 (MQ=60)
                                                               CGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                              <  SRR4302532.71988/94‑1 (MQ=60)
                                                               CGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                              <  SRR4302532.1662789/94‑1 (MQ=60)
                                                                 AGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                            >  SRR4302532.2281443/1‑94 (MQ=60)
                                                                 AGATTGCGGAGAGCTTCTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                            >  SRR4302532.4675460/1‑94 (MQ=60)
                                                                  GATTGCGGAGAGCTTTTTTAACTCCGCGTGCTGTCGTTTATTTGGCCACCGCCCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                           <  SRR4302532.1006806/94‑1 (MQ=60)
                                                                  GATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                           <  SRR4302532.3288346/94‑1 (MQ=60)
                                                                     TGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAG                        >  SRR4302532.5554532/1‑94 (MQ=60)
                                                                     TGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATGTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAG                        <  SRR4302532.2926025/94‑1 (MQ=60)
                                                                      GCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGC                       <  SRR4302532.3050242/94‑1 (MQ=60)
                                                                      GCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGC                       <  SRR4302532.5639209/94‑1 (MQ=60)
                                                                      GCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGC                       <  SRR4302532.2406041/94‑1 (MQ=60)
                                                                       CGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                      >  SRR4302532.5533471/1‑94 (MQ=60)
                                                                        GGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGC                     >  SRR4302532.4772271/1‑94 (MQ=60)
                                                                         GAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCC                    <  SRR4302532.4948714/94‑1 (MQ=60)
                                                                         GAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCC                    >  SRR4302532.1816839/1‑94 (MQ=60)
                                                                          AGAGCTTTTTTAACTCCGCGTAGTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                   <  SRR4302532.2833673/94‑1 (MQ=60)
                                                                          AGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                   >  SRR4302532.5277602/1‑94 (MQ=60)
                                                                          AGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                   >  SRR4302532.4654596/1‑94 (MQ=60)
                                                                          AGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                   >  SRR4302532.6094609/1‑94 (MQ=60)
                                                                           GAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                  >  SRR4302532.769889/1‑94 (MQ=60)
                                                                            AGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                 <  SRR4302532.3396246/94‑1 (MQ=60)
                                                                            AGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                 >  SRR4302532.2261025/1‑94 (MQ=60)
                                                                              CTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTT               >  SRR4302532.5589049/1‑94 (MQ=60)
                                                                               TTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTC              <  SRR4302532.1252411/94‑1 (MQ=60)
                                                                                TTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCG             >  SRR4302532.1917449/1‑94 (MQ=60)
                                                                                TTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCG             >  SRR4302532.2193098/1‑94 (MQ=60)
                                                                                   TTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAC          <  SRR4302532.1138416/94‑1 (MQ=60)
                                                                                     AACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCA        >  SRR4302532.4995724/1‑94 (MQ=60)
                                                                                     AACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCA        >  SRR4302532.1099090/1‑94 (MQ=60)
                                                                                      ACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAT       >  SRR4302532.326028/1‑94 (MQ=60)
                                                                                       CTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGTGGCCTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGCACCATT      >  SRR4302532.5675553/1‑94 (MQ=60)
                                                                                       CTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT      >  SRR4302532.652441/1‑94 (MQ=60)
                                                                                         CCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCC    <  SRR4302532.3045236/94‑1 (MQ=60)
                                                                                          CGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGTCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCTTACCATTCCC   <  SRR4302532.2856694/94‑1 (MQ=60)
                                                                                          CGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC   <  SRR4302532.5433204/94‑1 (MQ=60)
                                                                                           GCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  <  SRR4302532.23778/94‑1 (MQ=60)
                                                                                           GCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  <  SRR4302532.5543220/94‑1 (MQ=60)
                                                                                            |                                                                                            
CGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCC  >  NC_000913/4216399‑4216583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑