Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,491	T→C	V106A (GTG→GCG)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,491	0	T	C	99.6%	959.4 / ‑6.8	263	V106A (GTG→GCG)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (123/139); total (123/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC  >  NC_000913/4216401‑4216580

 ggCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTa                                                                                        <  1:3614786/93‑1 (MQ=255)

   cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACt                                                                                      <  2:1524080/93‑1 (MQ=255)

   cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACt                                                                                      <  2:2881411/93‑1 (MQ=255)

   cATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACt                                                                                      >  2:6522079/1‑93 (MQ=255)

    aTTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTg                                                                                     >  2:2507252/1‑93 (MQ=255)

    aTTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTg                                                                                     >  2:4982633/1‑93 (MQ=255)

     tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGt                                                                                    <  2:3709005/93‑1 (MQ=255)

     tttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGt                                                                                    <  2:4306261/93‑1 (MQ=255)

      ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTCACTTCGCGTACTGTc                                                                                   >  1:1817474/1‑93 (MQ=255)

      ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTc                                                                                   <  2:979922/93‑1 (MQ=255)

      ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTc                                                                                   <  1:5328226/93‑1 (MQ=255)

      ttttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTc                                                                                   >  1:4191238/1‑93 (MQ=255)

       tttACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCg                                                                                  <  2:302392/93‑1 (MQ=255)

           ctacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTa                                                                              >  2:3306297/1‑93 (MQ=255)

            tacGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAt                                                                             >  1:5146555/1‑93 (MQ=255)

             acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAtt                                                                            <  1:2552769/93‑1 (MQ=255)

             acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAtt                                                                            <  1:4295516/93‑1 (MQ=255)

             acGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAtt                                                                            >  2:1271439/1‑93 (MQ=255)

                tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGa                                                                         >  2:4332791/1‑93 (MQ=255)

                tgtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGa                                                                         <  2:5609163/93‑1 (MQ=255)

                 gtgaaaGAGCTTTACGCCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGac                                                                        <  1:1202105/90‑1 (MQ=255)

                 gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGac                                                                        <  1:2208760/93‑1 (MQ=255)

                 gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGac                                                                        >  1:3236416/1‑93 (MQ=255)

                 gtTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGac                                                                        <  2:6136147/93‑1 (MQ=255)

                  ttAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCTCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGacc                                                                       <  1:1269061/93‑1 (MQ=255)

                  ttAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGacc                                                                       <  2:5395084/93‑1 (MQ=255)

                   tAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGacca                                                                      >  2:2129081/1‑93 (MQ=255)

                    aaaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccac                                                                     <  2:5682692/93‑1 (MQ=255)

                    aaaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccac                                                                     <  2:909899/93‑1 (MQ=255)

                    aaaGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGaccac                                                                     <  2:2354210/93‑1 (MQ=255)

                       gagCATTACTCCCGGCTGTTGCCGAATTGCCAGCGATACGAGATTGAGAAGAGCTTTTTTAACTCCGCATAATGACGGTTATTTGACCTCcgc                                                                  <  1:6647921/93‑1 (MQ=255)

                            ttACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACcgctcgct                                                             <  2:1134262/93‑1 (MQ=255)

                             tACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCtt                                                            >  1:5985137/1‑93 (MQ=255)

                              acacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTa                                                           >  2:6408100/1‑93 (MQ=255)

                              acacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTa                                                           <  2:203777/93‑1 (MQ=255)

                               cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTAc                                                          >  1:2121028/1‑93 (MQ=255)

                               cacCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTAc                                                          <  1:3085493/93‑1 (MQ=255)

                                acCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACt                                                         >  2:5123041/1‑93 (MQ=255)

                                 cccGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTc                                                        <  1:5887395/93‑1 (MQ=255)

                                    ggCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCg                                                     >  2:6004705/1‑93 (MQ=255)

                                    ggCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCg                                                     >  1:111052/1‑93 (MQ=255)

                                     gCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGa                                                    <  2:4764836/93‑1 (MQ=255)

                                      cTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAg                                                   >  2:1951648/1‑93 (MQ=255)

                                       tGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                  <  1:1855008/93‑1 (MQ=255)

                                        gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                 <  2:4098236/93‑1 (MQ=255)

                                        gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                 >  1:5812879/1‑93 (MQ=255)

                                        gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                 <  2:3894822/93‑1 (MQ=255)

                                        gTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCg                                                 >  2:3137995/1‑93 (MQ=255)

                                          tGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTCCTCCCGAGCGGc                                               >  1:4482948/1‑93 (MQ=255)

                                          tGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGc                                               <  2:1389261/93‑1 (MQ=255)

                                          tGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGc                                               <  2:5495962/93‑1 (MQ=255)

                                          tGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGc                                               >  1:3904562/1‑93 (MQ=255)

                                          tGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGc                                               >  2:3480304/1‑93 (MQ=255)

                                           gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCt                                              <  1:941753/93‑1 (MQ=255)

                                           gCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCt                                              >  1:4691757/1‑93 (MQ=255)

                                            ccGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCtt                                             >  2:6468234/1‑93 (MQ=255)

                                               gATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCttttt                                          >  1:1942176/1‑93 (MQ=255)

                                                aTTTCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                         <  2:1730747/93‑1 (MQ=255)

                                                aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                         >  1:4592702/1‑93 (MQ=255)

                                                aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                         >  2:5590400/1‑93 (MQ=255)

                                                aTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTa                                         >  1:1579465/1‑93 (MQ=255)

                                                 ttACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTAt                                        <  2:601361/93‑1 (MQ=255)

                                                  tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                       <  2:6351563/93‑1 (MQ=255)

                                                  tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATc                                       <  1:2833634/93‑1 (MQ=255)

                                                  tACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCCTTTTATc                                       >  2:6569657/1‑93 (MQ=255)

                                                   aCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCt                                      <  1:4906600/93‑1 (MQ=255)

                                                   aCCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCt                                      <  1:2355955/93‑1 (MQ=255)

                                                    cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                     <  1:3105633/93‑1 (MQ=255)

                                                    cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCtt                                     <  2:478989/93‑1 (MQ=255)

                                                      cgcgCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTa                                   >  2:6279279/1‑93 (MQ=255)

                                                         gcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGct                                <  2:5749068/93‑1 (MQ=255)

                                                           ttCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                              >  1:3698764/1‑93 (MQ=255)

                                                           ttCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGctct                              >  1:3855275/1‑93 (MQ=255)

                                                             cGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCa                            >  1:1463229/1‑93 (MQ=255)

                                                             cGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCa                            <  1:6483512/93‑1 (MQ=255)

                                                             cGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCa                            <  1:4517146/93‑1 (MQ=255)

                                                              gagaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAg                           <  2:789128/93‑1 (MQ=255)

                                                              gagaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAg                           <  2:2856388/93‑1 (MQ=255)

                                                               agaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGc                          >  1:6445997/1‑93 (MQ=255)

                                                               agaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGc                          >  2:1901372/1‑93 (MQ=255)

                                                               agaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGc                          >  2:2798591/1‑93 (MQ=255)

                                                                gaTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGcc                         >  2:4619179/1‑93 (MQ=255)

                                                                 aTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                        <  2:3947897/93‑1 (MQ=255)

                                                                 aTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                        >  1:815502/1‑93 (MQ=255)

                                                                 aTTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCa                        <  1:3739529/93‑1 (MQ=255)

                                                                     cGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCGCCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAgc                    <  1:3345573/93‑1 (MQ=255)

                                                                        agagCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgcc                 <  1:727344/93‑1 (MQ=255)

                                                                         gagCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                <  1:6184319/93‑1 (MQ=255)

                                                                         gagCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccg                >  2:3268847/1‑93 (MQ=255)

                                                                          agCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGcgccgc               <  2:3550596/93‑1 (MQ=255)

                                                                            cTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCtt             >  1:4090808/1‑93 (MQ=255)

                                                                              tttttAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTc           <  2:4067906/93‑1 (MQ=255)

                                                                                  tAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAc       >  1:2745167/1‑93 (MQ=255)

                                                                                   aaCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc      <  2:1559972/93‑1 (MQ=255)

                                                                                   aaCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAcc      >  2:782408/1‑93 (MQ=255)

                                                                                    aCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa     >  1:6023005/1‑93 (MQ=255)

                                                                                    aCTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCa     >  1:853386/1‑93 (MQ=255)

                                                                                      tCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt   <  1:2257948/93‑1 (MQ=255)

                                                                                      tCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAtt   >  1:6240779/1‑93 (MQ=255)

                                                                                       ccGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTc  >  1:1043242/1‑93 (MQ=255)

CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC  >  NC_000913/4216401‑4216580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCG  >  NC_000913/4216398‑4216584

ACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGC                                                                                               >  SRR4302547.6345265/1‑94 (MQ=60)

   CGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTA                                                                                            <  SRR4302547.4512315/94‑1 (MQ=60)

    GGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTAC                                                                                           >  SRR4302547.3224620/1‑94 (MQ=60)

    GGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTAC                                                                                           <  SRR4302547.3688536/94‑1 (MQ=60)

     GCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACT                                                                                          >  SRR4302547.5702697/1‑94 (MQ=60)

     GCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACT                                                                                          <  SRR4302547.3725404/94‑1 (MQ=60)

       ATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGT                                                                                        >  SRR4302547.3813526/1‑94 (MQ=60)

       ATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGT                                                                                        >  SRR4302547.6345171/1‑94 (MQ=60)

        TTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTCACTTCGCGTACTGTC                                                                                       >  SRR4302547.1853392/1‑94 (MQ=60)

        TTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTC                                                                                       >  SRR4302547.4276717/1‑94 (MQ=60)

         TTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCG                                                                                      <  SRR4302547.5438055/94‑1 (MQ=60)

           TTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGT                                                                                    >  SRR4302547.1290781/1‑94 (MQ=60)

             ACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTA                                                                                  >  SRR4302547.2816391/1‑94 (MQ=60)

              CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                 <  SRR4302547.4651256/94‑1 (MQ=60)

              CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                 >  SRR4302547.5252552/1‑94 (MQ=60)

              CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                 >  SRR4302547.6084499/1‑94 (MQ=60)

              CTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTAT                                                                                 <  SRR4302547.6005866/94‑1 (MQ=60)

               TACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATT                                                                                <  SRR4302547.6716243/94‑1 (MQ=60)

               TACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATT                                                                                <  SRR4302547.2322062/94‑1 (MQ=60)

               TACGTGTTAAAGAGCATTACACCCGGCTGTTACCGTATTACTCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATT                                                                                <  SRR4302547.905735/94‑1 (MQ=60)

                ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTT                                                                               <  SRR4302547.4383828/94‑1 (MQ=60)

                ACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTT                                                                               <  SRR4302547.2604320/94‑1 (MQ=60)

                 CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTG                                                                              <  SRR4302547.5400165/94‑1 (MQ=60)

                 CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTG                                                                              <  SRR4302547.2808435/94‑1 (MQ=60)

                 CGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTG                                                                              >  SRR4302547.6356583/1‑94 (MQ=60)

                  GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGA                                                                             <  SRR4302547.3150702/94‑1 (MQ=60)

                  GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGA                                                                             <  SRR4302547.6125557/94‑1 (MQ=60)

                  GTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGA                                                                             >  SRR4302547.2776960/1‑94 (MQ=60)

                   TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGAC                                                                            >  SRR4302547.5095563/1‑94 (MQ=60)

                   TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGAC                                                                            >  SRR4302547.3301015/1‑94 (MQ=60)

                   TGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGAC                                                                            >  SRR4302547.2757458/1‑94 (MQ=60)

                    GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                           <  SRR4302547.2253167/94‑1 (MQ=60)

                    GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                           >  SRR4302547.2279121/1‑94 (MQ=60)

                    GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                           >  SRR4302547.3239800/1‑94 (MQ=60)

                    GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                           <  SRR4302547.1607952/94‑1 (MQ=60)

                    GTGAAAGAGCTTTACGCCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACC                                                                           <  SRR4302547.1226366/94‑1 (MQ=60)

                     TTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCTCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCA                                                                          <  SRR4302547.1294618/94‑1 (MQ=60)

                     TTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCA                                                                          >  SRR4302547.4348895/1‑94 (MQ=60)

                      TAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCAC                                                                         <  SRR4302547.1903240/94‑1 (MQ=60)

                        AAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCG                                                                       <  SRR4302547.4390988/94‑1 (MQ=60)

                           AGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTC                                                                    <  SRR4302547.809165/94‑1 (MQ=60)

                               TTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTT                                                                <  SRR4302547.835280/94‑1 (MQ=60)

                               TTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTT                                                                >  SRR4302547.3946821/1‑94 (MQ=60)

                               TTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTT                                                                >  SRR4302547.6109300/1‑94 (MQ=60)

                               TTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTT                                                                <  SRR4302547.3131681/94‑1 (MQ=60)

                                 TCACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGGTCGCTTAC                                                              <  SRR4302547.4589612/94‑1 (MQ=60)

                                 ACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTAC                                                              >  SRR4302547.2163305/1‑94 (MQ=60)

                                  CACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACT                                                             <  SRR4302547.3146726/94‑1 (MQ=60)

                                  CACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACT                                                             >  SRR4302547.874906/1‑94 (MQ=60)

                                   ACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTC                                                            >  SRR4302547.1598213/1‑94 (MQ=60)

                                    CCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCC                                                           >  SRR4302547.2281891/1‑94 (MQ=60)

                                    CCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCC                                                           <  SRR4302547.6009280/94‑1 (MQ=60)

                                     CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                          >  SRR4302547.2246214/1‑94 (MQ=60)

                                     CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                          <  SRR4302547.3826476/94‑1 (MQ=60)

                                     CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                          >  SRR4302547.6030868/1‑94 (MQ=60)

                                     CCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCC                                                          <  SRR4302547.1257154/94‑1 (MQ=60)

                                      CGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCG                                                         >  SRR4302547.113078/1‑94 (MQ=60)

                                         CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                      <  SRR4302547.4099771/94‑1 (MQ=60)

                                         CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                      <  SRR4302547.2025377/94‑1 (MQ=60)

                                         CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                      <  SRR4302547.3370539/94‑1 (MQ=60)

                                         CTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGC                                                      >  SRR4302547.2435833/1‑94 (MQ=60)

                                          TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                     <  SRR4302547.1298054/94‑1 (MQ=60)

                                          TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                     <  SRR4302547.1888024/94‑1 (MQ=60)

                                          TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                     <  SRR4302547.1891661/94‑1 (MQ=60)

                                          TGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCG                                                     >  SRR4302547.5933154/1‑94 (MQ=60)

                                           GTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGG                                                    <  SRR4302547.4788846/94‑1 (MQ=60)

                                            TTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTCCTCCCGAGCGGC                                                   >  SRR4302547.4575562/1‑94 (MQ=60)

                                            TTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGC                                                   >  SRR4302547.3983964/1‑94 (MQ=60)

                                            TTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGC                                                   >  SRR4302547.6449061/1‑94 (MQ=60)

                                             TGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCT                                                  >  SRR4302547.4788928/1‑94 (MQ=60)

                                              GCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTT                                                 <  SRR4302547.960004/94‑1 (MQ=60)

                                               CCGGAGTACCCGCGCTTCGAGATTCCGGAGAGCTTTTTTAACTCCGCGTACGGTCGGTTATTTGACCACCGCTCGCTTCCTCCCGCGCGGCTTT                                                <  SRR4302547.5443204/94‑1 (MQ=60)

                                                 GGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTT                                              >  SRR4302547.1980683/1‑94 (MQ=60)

                                                  GATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTA                                             >  SRR4302547.1610900/1‑94 (MQ=60)

                                                  GATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTA                                             >  SRR4302547.1944661/1‑94 (MQ=60)

                                                  GATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTA                                             >  SRR4302547.4687737/1‑94 (MQ=60)

                                                    TTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATC                                           <  SRR4302547.6682591/94‑1 (MQ=60)

                                                     TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                          <  SRR4302547.4121291/94‑1 (MQ=60)

                                                     TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                          <  SRR4302547.2890292/94‑1 (MQ=60)

                                                     TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCT                                          <  SRR4302547.1196480/94‑1 (MQ=60)

                                                      ACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTT                                         <  SRR4302547.5008211/94‑1 (MQ=60)

                                                      ACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTT                                         <  SRR4302547.2403695/94‑1 (MQ=60)

                                                       CCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTT                                        <  SRR4302547.3167184/94‑1 (MQ=60)

                                                         CGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAG                                      <  SRR4302547.1681905/94‑1 (MQ=60)

                                                           CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCT                                    <  SRR4302547.732177/94‑1 (MQ=60)

                                                             CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                  >  SRR4302547.3774255/1‑94 (MQ=60)

                                                             CTTCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCT                                  >  SRR4302547.3933748/1‑94 (MQ=60)

                                                               TCGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCA                                >  SRR4302547.1492463/1‑94 (MQ=60)

                                                                CGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                               <  SRR4302547.6284700/94‑1 (MQ=60)

                                                                CGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                               <  SRR4302547.6618488/94‑1 (MQ=60)

                                                                CGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                               >  SRR4302547.5886440/1‑94 (MQ=60)

                                                                CGAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAG                               <  SRR4302547.4610465/94‑1 (MQ=60)

                                                                 GAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGC                              >  SRR4302547.6580308/1‑94 (MQ=60)

                                                                 GAGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGC                              <  SRR4302547.694461/94‑1 (MQ=60)

                                                                  AGATTGCGTAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                             >  SRR4302547.459586/1‑94 (MQ=60)

                                                                  AGATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCC                             >  SRR4302547.3792576/1‑94 (MQ=60)

                                                                   GATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCA                            >  SRR4302547.830990/1‑94 (MQ=60)

                                                                    ATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAG                           <  SRR4302547.4983760/94‑1 (MQ=60)

                                                                    ATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAG                           <  SRR4302547.3815875/94‑1 (MQ=60)

                                                                    ATTGCGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAG                           <  SRR4302547.2054347/94‑1 (MQ=60)

                                                                        CGGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCGCCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCG                       <  SRR4302547.3413334/94‑1 (MQ=60)

                                                                         GGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGC                      >  SRR4302547.4905443/1‑94 (MQ=60)

                                                                         GGAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGC                      >  SRR4302547.4710437/1‑94 (MQ=60)

                                                                          GAGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCC                     <  SRR4302547.5257228/94‑1 (MQ=60)

                                                                           AGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                    >  SRR4302547.6481228/1‑94 (MQ=60)

                                                                           AGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                    <  SRR4302547.3038677/94‑1 (MQ=60)

                                                                           AGAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCG                    <  SRR4302547.740907/94‑1 (MQ=60)

                                                                            GAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                   >  SRR4302547.1196414/1‑94 (MQ=60)

                                                                            GAGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGC                   <  SRR4302547.6312675/94‑1 (MQ=60)

                                                                             AGCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCT                  >  SRR4302547.6128890/1‑94 (MQ=60)

                                                                              GCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTT                 >  SRR4302547.4174002/1‑94 (MQ=60)

                                                                              GCTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTT                 <  SRR4302547.4193404/94‑1 (MQ=60)

                                                                               CTTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTT                <  SRR4302547.1734893/94‑1 (MQ=60)

                                                                                TTTTTTAACTCCGCGTCCTGTCGGTTATTTGACCACCGCTCGCTTCCTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTC               <  SRR4302547.3689122/94‑1 (MQ=60)

                                                                                TTTTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCTGCTTTC               >  SRR4302547.976233/1‑94 (MQ=60)

                                                                                  TTTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGT             >  SRR4302547.3310418/1‑94 (MQ=60)

                                                                                   TTTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTA            >  SRR4302547.4301052/1‑94 (MQ=60)

                                                                                    TTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAC           <  SRR4302547.6610267/94‑1 (MQ=60)

                                                                                    TTAACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTAC           >  SRR4302547.2800222/1‑94 (MQ=60)

                                                                                      AACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCA         >  SRR4302547.6148016/1‑94 (MQ=60)

                                                                                      AACTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCA         >  SRR4302547.869683/1‑94 (MQ=60)

                                                                                        CTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGGCAGAGCGCCGCTTTTGTAACATT       >  SRR4302547.2373693/1‑94 (MQ=60)

                                                                                        CTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT       >  SRR4302547.1083469/1‑94 (MQ=60)

                                                                                        CTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT       >  SRR4302547.6370428/1‑94 (MQ=60)

                                                                                        CTCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATT       <  SRR4302547.803350/94‑1 (MQ=60)

                                                                                         TCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC      <  SRR4302547.3545531/94‑1 (MQ=60)

                                                                                         TCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC      >  SRR4302547.1063832/1‑94 (MQ=60)

                                                                                         TCCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTC      <  SRR4302547.2303551/94‑1 (MQ=60)

                                                                                          CCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCC     <  SRR4302547.3951918/94‑1 (MQ=60)

                                                                                          CCGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCC     >  SRR4302547.6715553/1‑94 (MQ=60)

                                                                                           CGCGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCC    <  SRR4302547.3765038/94‑1 (MQ=60)

                                                                                             CGTACTGTCGGTTATTTGACCCCCGCTCTCTTACTCCCGAGCGACTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTACGTACCATTCCCCG  <  SRR4302547.4370280/94‑1 (MQ=60)

                                                                                             CGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCG  >  SRR4302547.1894763/1‑94 (MQ=60)

                                                                                             CGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCG  >  SRR4302547.6263124/1‑94 (MQ=60)

ACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCG  >  NC_000913/4216398‑4216584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`