Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,449,738 +G coding (1106/1254 nt) ytfR → YtfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,449,7371.G99.6% 837.5 / ‑5.1 231C369G (TGC→GGC) ytfRYtfR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (96/134);  minor base C (1/0);  total (97/134)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 251 total aligned reads displayed.

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824
                                                                                             |                                                                                       
gCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct                                                                                          <  2:1383126/93‑1 (MQ=255)
  ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑gg                                                                                       <  2:5900131/93‑2 (MQ=255)
  ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑gg                                                                                       >  1:6133342/1‑92 (MQ=255)
   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      >  1:123095/1‑91 (MQ=255)
   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      >  1:3124240/1‑91 (MQ=255)
   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      <  1:3953106/93‑3 (MQ=255)
       cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACg                                                                                   <  2:3008268/93‑1 (MQ=255)
          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTcc                                                                                <  1:1411071/93‑1 (MQ=255)
            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              <  1:589254/93‑1 (MQ=255)
            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              <  2:521133/93‑1 (MQ=255)
              gACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAtt                                                                            <  1:69532/93‑1 (MQ=255)
              gACAGGAAAACCGATGACATCATCGCTGCCGCCACGGTGCGAGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGATACGTCCCAtt                                                                            <  1:5463373/93‑1 (MQ=255)
                cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTc                                                                          <  2:1614635/93‑1 (MQ=255)
                 aGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCCTTTcc                                                                         >  2:1502137/1‑93 (MQ=255)
                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        <  2:1592690/93‑1 (MQ=255)
                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        >  1:740793/1‑93 (MQ=255)
                     aaaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCa                                                                     >  2:5833158/1‑93 (MQ=255)
                     aaaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCa                                                                     <  1:4510324/93‑1 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  >  2:4389087/1‑93 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  <  1:4214616/93‑1 (MQ=255)
                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 <  1:1975873/93‑1 (MQ=255)
                         cGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGa                                                                 <  2:3925965/93‑1 (MQ=255)
                          gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGaa                                                                <  2:2865642/93‑1 (MQ=255)
                          gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGaa                                                                >  2:4143390/1‑93 (MQ=255)
                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               >  1:3079860/1‑93 (MQ=255)
                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               <  1:2906950/93‑1 (MQ=255)
                           aTGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAc                                                               >  2:4955639/1‑93 (MQ=255)
                           aTGGCATCACCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGacc                                                               <  1:3845818/93‑3 (MQ=255)
                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              >  1:921360/1‑93 (MQ=255)
                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              <  2:4772309/93‑1 (MQ=255)
                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              <  2:189503/93‑1 (MQ=255)
                            tGGCATCATCGCCGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              <  2:4034639/93‑1 (MQ=255)
                             ggCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAg                                                             <  2:4728255/93‑1 (MQ=255)
                               catcatCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                           >  2:3481901/1‑93 (MQ=255)
                                atcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCaa                                                          <  2:2450767/93‑1 (MQ=255)
                                atcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCaa                                                          <  2:4447922/93‑1 (MQ=255)
                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  1:2519267/1‑93 (MQ=255)
                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  1:603563/1‑93 (MQ=255)
                                  catcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAaga                                                        <  2:6052864/93‑1 (MQ=255)
                                  catcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTATCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAaga                                                        <  2:3064451/93‑1 (MQ=255)
                                   atcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAagag                                                       <  2:5786490/93‑1 (MQ=255)
                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      >  2:3829354/1‑93 (MQ=255)
                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      <  1:2351242/93‑1 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  2:1037115/93‑1 (MQ=255)
                                      gCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAtt                                                    >  2:4539734/1‑93 (MQ=255)
                                         gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGcc                                                 <  2:716613/93‑1 (MQ=255)
                                         gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGcc                                                 <  2:5715829/93‑1 (MQ=255)
                                           cgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGa                                               <  2:5047510/93‑1 (MQ=255)
                                           cgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGa                                               >  2:5634150/1‑93 (MQ=255)
                                            gccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                              >  1:3755590/1‑93 (MQ=255)
                                             ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             >  2:628355/1‑93 (MQ=255)
                                               tCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                           >  2:3346936/1‑93 (MQ=255)
                                                 ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCTCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                         <  1:4109121/93‑1 (MQ=255)
                                                 ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                         <  2:4267753/93‑1 (MQ=255)
                                                   tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                       <  1:1428420/93‑1 (MQ=255)
                                                    gCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAt                                      >  2:517839/1‑93 (MQ=255)
                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     >  2:3751870/1‑93 (MQ=255)
                                                      gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                    <  1:1838025/93‑1 (MQ=255)
                                                      gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                    >  2:3383074/1‑93 (MQ=255)
                                                         aaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGcc                                 <  1:258426/93‑1 (MQ=255)
                                                             tatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                             <  1:3516764/93‑1 (MQ=255)
                                                              atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                            >  1:2098307/1‑93 (MQ=255)
                                                              atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                            <  2:5815893/93‑1 (MQ=255)
                                                              atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                            >  1:5585171/1‑93 (MQ=255)
                                                                 atcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         <  1:4885215/93‑1 (MQ=255)
                                                                 atcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         >  1:3035580/1‑93 (MQ=255)
                                                                  tcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCa                        >  1:5693368/1‑93 (MQ=255)
                                                                  accTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCa                        <  2:2231076/92‑1 (MQ=255)
                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  1:2691924/93‑1 (MQ=255)
                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  1:3700921/93‑1 (MQ=255)
                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  1:42940/93‑1 (MQ=255)
                                                                    cTCGCACTCCAGGCTCAGCGCGGCTGGCTACGTCCCCTTTCCCGCAAAGAACAGCAAGAGACTGCCGAACGATTTATCCGCCAGCTTCGCCtt                      >  1:5159371/1‑93 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     <  1:1127822/93‑1 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     >  2:937043/1‑93 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     <  1:3567148/93‑1 (MQ=255)
                                                                      cGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCg                    >  2:592163/1‑93 (MQ=255)
                                                                       gCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                   >  2:2580328/1‑93 (MQ=255)
                                                                       gCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                   >  2:2167279/1‑93 (MQ=255)
                                                                       ccgctcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                   <  1:1404396/90‑1 (MQ=255)
                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  <  2:4853487/93‑1 (MQ=255)
                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  >  1:3212163/1‑93 (MQ=255)
                                                                        ctccCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  <  1:5711160/93‑1 (MQ=255)
                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac               >  2:1744715/1‑93 (MQ=255)
                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac               >  1:6075070/1‑93 (MQ=255)
                                                                            cgcggCCCTGCGTGGCTGGCTCCGTCCCATCTCCCGCAACGAACAGCAAGAGATTGCGCAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              <  1:1297429/90‑1 (MQ=255)
                                                                            ccAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              >  2:3945330/1‑93 (MQ=255)
                                                                            ccAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              <  2:751412/93‑1 (MQ=255)
                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             <  1:4054428/93‑1 (MQ=255)
                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             <  1:2127027/93‑1 (MQ=255)
                                                                                   cAGCGCGGCTAGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       <  1:5187338/93‑1 (MQ=255)
                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     <  2:5476524/93‑1 (MQ=255)
                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  1:5303855/1‑93 (MQ=255)
                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  1:1583066/1‑93 (MQ=255)
                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  2:3676780/1‑93 (MQ=255)
                                                                                     gcgcGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGa     >  2:4087608/1‑93 (MQ=255)
                                                                                      cgcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaa    <  2:233639/93‑1 (MQ=255)
                                                                                       gcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaac   >  1:5044796/1‑93 (MQ=255)
                                                                                       gcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaac   <  1:3386109/93‑1 (MQ=255)
                                                                                       gcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaac   <  1:1752938/93‑1 (MQ=255)
                                                                                        cgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaaca  <  2:1300823/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  >  NC_000913/4449645‑4449831
                                                                                              |                                                                                             
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑G                                                                                               <  SRR4302532.4980565/94‑1 (MQ=60)
 CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                              <  SRR4302532.2633442/94‑1 (MQ=60)
 CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                              >  SRR4302532.6344941/1‑94 (MQ=60)
  TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑Ggc                                                                                             >  SRR4302532.3227937/1‑92 (MQ=60)
  TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑Ggc                                                                                             >  SRR4302532.127278/1‑92 (MQ=60)
  TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑Ggc                                                                                             >  SRR4302532.2663493/1‑92 (MQ=60)
   TCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑Ggct                                                                                            <  SRR4302532.4086320/94‑4 (MQ=60)
     TGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTAC                                                                                           <  SRR4302532.383516/94‑1 (MQ=60)
     TGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTAC                                                                                           <  SRR4302532.5881696/94‑1 (MQ=60)
       CCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGT                                                                                         >  SRR4302532.370329/1‑94 (MQ=60)
       CCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGT                                                                                         >  SRR4302532.3511832/1‑94 (MQ=60)
       CCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGT                                                                                         >  SRR4302532.3400171/1‑94 (MQ=60)
         CGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGAGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCC                                                                                       <  SRR4302532.4627320/94‑1 (MQ=60)
          GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCC                                                                                      <  SRR4302532.1458375/94‑1 (MQ=60)
          GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCC                                                                                      >  SRR4302532.2180054/1‑94 (MQ=60)
          CGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCC                                                                                      <  SRR4302532.3316008/94‑1 (MQ=60)
            AAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAT                                                                                    <  SRR4302532.609808/94‑1 (MQ=60)
            AAGACAGGAAAACCGATGCCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGGCCCAT                                                                                    <  SRR4302532.1177306/94‑1 (MQ=60)
             AGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATT                                                                                   >  SRR4302532.5722445/1‑94 (MQ=60)
             AGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATT                                                                                   <  SRR4302532.6092963/94‑1 (MQ=60)
              GACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTT                                                                                  >  SRR4302532.2307334/1‑94 (MQ=60)
              GACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTT                                                                                  <  SRR4302532.72015/94‑1 (MQ=60)
              GACAGGAAAACCGATGACATCATCGCTGCCGCCACGGTGCGAGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGATACGTCCCATTT                                                                                  <  SRR4302532.5652792/94‑1 (MQ=60)
                CAGGAAAACCGAGGGCATCATCGATGCCGCCTCGGTGCGGGAAAATTTAATCCTCGCTCTCCAGGCCCAGCGCCGCTGGCGACGTCCCCTTTCC                                                                                >  SRR4302532.3750371/1‑94 (MQ=60)
                 AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                               <  SRR4302532.3203384/94‑1 (MQ=60)
                 AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                               >  SRR4302532.766068/1‑94 (MQ=60)
                    AAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCA                                                                            <  SRR4302532.2830605/94‑1 (MQ=60)
                    AAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCA                                                                            >  SRR4302532.3934721/1‑94 (MQ=60)
                     AAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAA                                                                           <  SRR4302532.4664317/94‑1 (MQ=60)
                     AAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAA                                                                           >  SRR4302532.6085177/1‑94 (MQ=60)
                       ACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAG                                                                         <  SRR4302532.1334768/94‑1 (MQ=60)
                        CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                        <  SRR4302532.4356075/94‑1 (MQ=60)
                         CGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAA                                                                       <  SRR4302532.2041501/94‑1 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                      >  SRR4302532.2995831/1‑94 (MQ=60)
                          GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                      >  SRR4302532.3181681/1‑94 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     >  SRR4302532.316087/1‑94 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     <  SRR4302532.3003914/94‑1 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     <  SRR4302532.2054350/94‑1 (MQ=60)
                           ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                     >  SRR4302532.952907/1‑94 (MQ=60)
                           ATGGCATCACCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGACCA                                                                     <  SRR4302532.3975675/94‑1 (MQ=60)
                             GGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGC                                                                   >  SRR4302532.1604672/1‑94 (MQ=60)
                                ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                >  SRR4302532.2603326/1‑94 (MQ=60)
                                ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                                >  SRR4302532.624653/1‑94 (MQ=60)
                                   ATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGA                                                             >  SRR4302532.411695/1‑94 (MQ=60)
                                    TCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAT                                                            <  SRR4302532.2429690/94‑1 (MQ=60)
                                      GCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                          <  SRR4302532.649001/94‑1 (MQ=60)
                                        TGCCGCCTGGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCC                                                        >  SRR4302532.6239100/1‑94 (MQ=60)
                                          CCGCCTCGGTGCGGCAAAATATCATCCTCGCTCTCCAGGCCCAGTGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGA                                                      <  SRR4302532.631117/94‑1 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCCAAGAACAGCAAGAGAATGCCGAA                                                     >  SRR4302532.977744/1‑94 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                     >  SRR4302532.2066015/1‑94 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                     >  SRR4302532.940034/1‑94 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                     >  SRR4302532.3881900/1‑94 (MQ=60)
                                           CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                     >  SRR4302532.2673009/1‑94 (MQ=60)
                                            GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                    >  SRR4302532.834653/1‑94 (MQ=60)
                                              CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                  >  SRR4302532.3674423/1‑94 (MQ=60)
                                              CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGACGAACGC                                                  >  SRR4302532.5162690/1‑94 (MQ=60)
                                               TCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCT                                                 <  SRR4302532.4365845/94‑1 (MQ=60)
                                                 GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCTCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                               <  SRR4302532.4247048/94‑1 (MQ=60)
                                                   TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                             <  SRR4302532.1476426/94‑1 (MQ=60)
                                                   TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                             <  SRR4302532.1476427/94‑1 (MQ=60)
                                                     CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                           <  SRR4302532.1427455/94‑1 (MQ=60)
                                                      GGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAGGCTTTATCCG                                          >  SRR4302532.5137529/1‑94 (MQ=60)
                                                      GGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCG                                          <  SRR4302532.1899106/94‑1 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       <  SRR4302532.267390/94‑1 (MQ=60)
                                                         AAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCA                                       <  SRR4302532.5411741/94‑1 (MQ=60)
                                                          AAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAG                                      <  SRR4302532.669816/94‑1 (MQ=60)
                                                            ATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCT                                    >  SRR4302532.3136971/1‑94 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   >  SRR4302532.2168308/1‑94 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   >  SRR4302532.5779068/1‑94 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   >  SRR4302532.3888899/1‑94 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   <  SRR4302532.3634507/94‑1 (MQ=60)
                                                             TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                   <  SRR4302532.1458219/94‑1 (MQ=60)
                                                              ATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTG                                  >  SRR4302532.4215556/1‑94 (MQ=60)
                                                               TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                 <  SRR4302532.5549592/94‑1 (MQ=60)
                                                               TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                 <  SRR4302532.1853686/94‑1 (MQ=60)
                                                                CATCCTCTCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                                <  SRR4302532.1642732/94‑1 (MQ=60)
                                                                CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                                >  SRR4302532.2521109/1‑94 (MQ=60)
                                                                CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                                >  SRR4302532.4155999/1‑94 (MQ=60)
                                                                CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                                >  SRR4302532.6257762/1‑94 (MQ=60)
                                                                CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                                >  SRR4302532.3135732/1‑94 (MQ=60)
                                                                 ATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                               <  SRR4302532.5053149/94‑1 (MQ=60)
                                                                 ATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                               >  SRR4302532.5892005/1‑94 (MQ=60)
                                                                 ATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                               <  SRR4302532.4491549/94‑1 (MQ=60)
                                                                   CCTCGCACTCCAGGCTCAGCGCGGCTGGCTACGTCCCCTTTCCCGCAAAGAACAGCAAGAGACTGCCGAACGATTTATCCGCCAGCTTCGCCTT                             >  SRR4302532.5336912/1‑94 (MQ=60)
                                                                    CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                            <  SRR4302532.44476/94‑1 (MQ=60)
                                                                    CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                            <  SRR4302532.2782340/94‑1 (MQ=60)
                                                                    CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                            <  SRR4302532.3825175/94‑1 (MQ=60)
                                                                     TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                           <  SRR4302532.1165444/94‑1 (MQ=60)
                                                                     TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                           <  SRR4302532.745660/94‑1 (MQ=60)
                                                                     TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                           <  SRR4302532.3686515/94‑1 (MQ=60)
                                                                     TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                           <  SRR4302532.1827378/94‑1 (MQ=60)
                                                                     CCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                           <  SRR4302532.1317110/94‑1 (MQ=60)
                                                                       ccgCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCA                         <  SRR4302532.1451437/91‑1 (MQ=60)
                                                                       GCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCA                         >  SRR4302532.3318816/1‑94 (MQ=60)
                                                                       GCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCA                         <  SRR4302532.1451438/94‑1 (MQ=60)
                                                                        CTCCCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                        <  SRR4302532.5910227/94‑1 (MQ=60)
                                                                         TCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACA                       <  SRR4302532.4266944/94‑1 (MQ=60)
                                                                          CTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                      >  SRR4302532.6284870/1‑94 (MQ=60)
                                                                          CTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                      >  SRR4302532.6284871/1‑94 (MQ=60)
                                                                             CAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                   <  SRR4302532.4190796/94‑1 (MQ=60)
                                                                             CAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                   <  SRR4302532.1559435/94‑1 (MQ=60)
                                                                             CAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                   <  SRR4302532.2198096/94‑1 (MQ=60)
                                                                             CAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                   >  SRR4302532.3287237/1‑94 (MQ=60)
                                                                             CAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTT                   <  SRR4302532.2353950/94‑1 (MQ=60)
                                                                              AGGCCCAGCGCGGTTGGGTACGGCCCCTTTCCCGCAAAGAACAGCAAGAGATTGCCGAAACCTTTATCCGtcagcgtagcatttgcacacgtta                  >  SRR4302532.2253716/1‑70 (MQ=27)
                                                                              AGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTC                  >  SRR4302532.590803/1‑94 (MQ=60)
                                                                               GGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                 <  SRR4302532.3106306/94‑1 (MQ=60)
                                                                               GGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                 <  SRR4302532.5286332/94‑1 (MQ=60)
                                                                               GGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                 <  SRR4302532.3556000/94‑1 (MQ=60)
                                                                                  CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT              <  SRR4302532.5803464/94‑1 (MQ=60)
                                                                                  CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT              <  SRR4302532.5347249/94‑1 (MQ=60)
                                                                                  CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT              <  SRR4302532.431723/94‑1 (MQ=60)
                                                                                   CAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG             <  SRR4302532.5366087/94‑1 (MQ=60)
                                                                                   CAGCGCGGCTAGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG             <  SRR4302532.5366088/94‑1 (MQ=60)
                                                                                    AGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGA            >  SRR4302532.1636655/1‑94 (MQ=60)
                                                                                    AGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGA            >  SRR4302532.5487180/1‑94 (MQ=60)
                                                                                    AGCGCGGCTGGCTACGTCCCATTTCCCCCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCCTGGGATTCGCACACgtgcacatca            >  SRR4302532.1636654/1‑84 (MQ=60)
                                                                                      CGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAAC          <  SRR4302532.4014717/94‑1 (MQ=60)
                                                                                      CGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAAC          >  SRR4302532.5217853/1‑94 (MQ=60)
                                                                                       GCGGCTGGCTTCGTACCATTTACCGCAAAGACCAGCAATAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA         <  SRR4302532.5496782/94‑1 (MQ=60)
                                                                                       GCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA         <  SRR4302532.3499357/94‑1 (MQ=60)
                                                                                       GCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA         >  SRR4302532.3708460/1‑94 (MQ=60)
                                                                                       GCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA         <  SRR4302532.1811817/94‑1 (MQ=60)
                                                                                         GGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAC       <  SRR4302532.3013118/94‑1 (MQ=60)
                                                                                         GGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAC       >  SRR4302532.3105826/1‑94 (MQ=60)
                                                                                          GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC      <  SRR4302532.1897913/94‑1 (MQ=60)
                                                                                           ctgGCTACGTCCCATTTTCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCG     >  SRR4302532.4083967/4‑94 (MQ=60)
                                                                                              GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  <  SRR4302532.2602991/94‑1 (MQ=60)
                                                                                              GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  <  SRR4302532.1224947/94‑1 (MQ=60)
                                                                                              |                                                                                             
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATT  >  NC_000913/4449645‑4449831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑