Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,583,050	G→A	Q446* (CAA→TAA)	hsdR ←	endonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,583,050	0	G	A	99.6%	984.8 / NA	264	Q446* (CAA→TAA)	hsdR	endonuclease R
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (147/115); minor base C (1/0); total (148/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 266 total aligned reads displayed.

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGC  >  NC_000913/4582960‑4583139

tttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACg                                                                                         <  2:4718987/93‑1 (MQ=255)

tttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACg                                                                                         <  2:2318606/93‑1 (MQ=255)

 ttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACgg                                                                                        <  1:5906136/93‑1 (MQ=255)

  tCAACCGCGCGGCCGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGt                                                                                       <  2:3978488/93‑1 (MQ=255)

  tCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGt                                                                                       <  1:3039358/93‑1 (MQ=255)

    acccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                     <  2:2992984/91‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                     <  2:3947717/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                     <  1:5759017/93‑1 (MQ=255)

      ccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                   <  1:1741084/93‑1 (MQ=255)

       cgcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCt                                                                                  <  2:3197992/93‑1 (MQ=255)

        gcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTg                                                                                 >  2:4223750/1‑93 (MQ=255)

        gcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTg                                                                                 >  2:1874077/1‑93 (MQ=255)

          gcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGcc                                                                               <  2:6071593/93‑1 (MQ=255)

           cgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCg                                                                              >  1:2032855/1‑93 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                             <  1:5698042/93‑1 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                             <  1:409252/93‑1 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                             <  2:2850988/93‑1 (MQ=255)

            cgACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                             <  1:1582192/92‑1 (MQ=255)

              aCGTCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGc                                                                           <  1:580713/93‑1 (MQ=255)

                 gCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                        >  2:2915868/1‑93 (MQ=255)

                   aTCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTc                                                                      <  1:1969032/93‑1 (MQ=255)

                   aTCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTc                                                                      >  1:3472458/1‑93 (MQ=255)

                   aTCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTc                                                                      <  1:3158747/93‑1 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                     >  1:1216835/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                     >  2:4088335/1‑93 (MQ=255)

                     cTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCca                                                                    >  1:3371944/1‑93 (MQ=255)

                      ttCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcag                                                                   >  2:1031107/1‑93 (MQ=255)

                       tCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagc                                                                  >  1:3626485/1‑93 (MQ=255)

                       tCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagc                                                                  <  1:2555708/93‑1 (MQ=255)

                         cTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagcag                                                                <  2:3889551/93‑1 (MQ=255)

                          tGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCagcagc                                                               >  1:5556956/1‑93 (MQ=255)

                          tGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCagcagc                                                               >  2:5591261/1‑93 (MQ=255)

                          tGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCagcagc                                                               >  2:4177677/1‑93 (MQ=255)

                           gATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGct                                                              >  2:2514109/1‑93 (MQ=255)

                             taataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctct                                                            <  1:2904983/93‑1 (MQ=255)

                               ataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTc                                                          <  1:641996/93‑1 (MQ=255)

                                taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                         >  2:5853133/1‑93 (MQ=255)

                                  aCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTCCGGTTCGCTGCCGAGCATTTTCAGCATCTCTTCCgg                                                       >  1:4284243/1‑93 (MQ=255)

                                  aCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCgg                                                       <  1:5115339/93‑1 (MQ=255)

                                  aCGCAGGCCCAGCTCGCTCATGCCAGGCTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCgg                                                       <  1:3914322/93‑1 (MQ=255)

                                     cAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCg                                                    >  2:16162/1‑93 (MQ=255)

                                          ccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGcc                                               >  1:4649776/1‑93 (MQ=255)

                                          ccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGcc                                               >  1:2289052/1‑93 (MQ=255)

                                           cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGccc                                              >  2:4629319/1‑92 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGGTCTTCCGGGCGGTTCCACt                                            >  1:3813819/1‑93 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                            >  2:1910906/1‑93 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                            <  1:1701939/93‑1 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                          >  2:5352965/1‑93 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                          >  2:4282859/1‑93 (MQ=255)

                                                 gctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCggg                                        <  1:4590179/93‑1 (MQ=255)

                                                 gctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCggg                                        <  1:7072/93‑1 (MQ=255)

                                                   tcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTa                                      >  1:5074991/1‑93 (MQ=255)

                                                   tcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTa                                      >  2:3776885/1‑93 (MQ=255)

                                                    cATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTaa                                     <  2:1163328/93‑1 (MQ=255)

                                                       gCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc                                  >  2:2804551/1‑93 (MQ=255)

                                                       gCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc                                  <  2:2130608/93‑1 (MQ=255)

                                                       gCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc                                  >  2:2744794/1‑93 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                 >  2:2000655/1‑93 (MQ=255)

                                                         cAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCtt                                <  2:359595/93‑1 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                               >  2:3419155/1‑93 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                               <  1:435934/93‑1 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                               >  1:1005829/1‑93 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                               >  1:1376609/1‑93 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                              <  2:5301115/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                              >  2:4061000/1‑93 (MQ=255)

                                                            ggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCg                             >  1:942364/1‑93 (MQ=255)

                                                            ggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCg                             >  1:5643959/1‑93 (MQ=255)

                                                            ggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGCAAGGCTTTCg                             >  1:4017170/1‑93 (MQ=255)

                                                             gTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGa                            >  2:2681756/1‑93 (MQ=255)

                                                                 tCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACata                        >  1:1304411/1‑93 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                      <  2:1420219/93‑1 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    >  2:3060611/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    >  1:1121407/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    <  1:2367351/93‑1 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    <  1:4878991/93‑1 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGAGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    <  1:4127292/93‑1 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTACCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    >  1:449940/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   >  2:1675120/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   >  2:5239357/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   <  2:3321184/93‑1 (MQ=255)

                                                                        aCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggg                 <  2:3837421/93‑1 (MQ=255)

                                                                        aCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggg                 <  1:3761942/93‑1 (MQ=255)

                                                                        aCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggg                 <  1:5001136/93‑1 (MQ=255)

                                                                        aCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggg                 >  2:1272083/1‑93 (MQ=255)

                                                                         ccACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggga                >  1:1402116/1‑92 (MQ=255)

                                                                         ccACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGt                <  2:4977737/93‑1 (MQ=255)

                                                                         ccACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGt                <  1:1057061/93‑1 (MQ=255)

                                                                          cACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTa               >  2:2237944/1‑93 (MQ=255)

                                                                          cACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTa               <  1:6013853/93‑1 (MQ=255)

                                                                          cACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTa               <  1:4950825/93‑1 (MQ=255)

                                                                           aCTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAGGGCTTTCGACATATTGGGGGTAt              >  2:4788585/1‑93 (MQ=255)

                                                                           aCTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTAt              >  2:907214/1‑93 (MQ=255)

                                                                           aCTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTACGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTAt              >  2:5732768/1‑93 (MQ=255)

                                                                            cTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATc             >  2:3512108/1‑93 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg          <  2:4414913/93‑1 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca        <  1:422055/93‑1 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca        >  2:750201/1‑93 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca        >  1:18613/1‑93 (MQ=255)

                                                                                  cTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcac       >  1:995324/1‑93 (MQ=255)

                                                                                     tttttACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTc    <  1:5892549/93‑1 (MQ=255)

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGC  >  NC_000913/4582960‑4583139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  >  NC_000913/4582957‑4583143

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTA                                                                                               >  SRR4302532.4937068/1‑94 (MQ=60)

 CTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTAC                                                                                              >  SRR4302532.3295607/1‑94 (MQ=60)

 CTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTAC                                                                                              <  SRR4302532.2947597/94‑1 (MQ=60)

  TTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACG                                                                                             <  SRR4302532.1820964/94‑1 (MQ=60)

    TTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGGTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGT                                                                                           <  SRR4302532.6110680/94‑1 (MQ=60)

    TTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGT                                                                                           <  SRR4302532.6110678/94‑1 (MQ=60)

     TCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTT                                                                                          <  SRR4302532.3400497/94‑1 (MQ=60)

     TCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTT                                                                                          <  SRR4302532.3139637/94‑1 (MQ=60)

      CAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTC                                                                                         <  SRR4302532.2037905/94‑1 (MQ=60)

       AACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCG                                                                                        >  SRR4302532.5881236/1‑94 (MQ=60)

       AACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCG                                                                                        <  SRR4302532.5959458/94‑1 (MQ=60)

         CCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTTCGGTTCGCT                                                                                      <  SRR4302532.2151685/94‑1 (MQ=60)

         CCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCT                                                                                      <  SRR4302532.1799676/94‑1 (MQ=60)

         CCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCT                                                                                      >  SRR4302532.4808323/1‑94 (MQ=60)

         CCGCGCGGACGCCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCT                                                                                      <  SRR4302532.4046634/94‑1 (MQ=60)

             GCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCG                                                                                  >  SRR4302532.2100596/1‑94 (MQ=60)

               GGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                <  SRR4302532.423527/94‑1 (MQ=60)

               GGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                <  SRR4302532.5896802/94‑1 (MQ=60)

               GGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                >  SRR4302532.1106138/1‑94 (MQ=60)

               CGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                <  SRR4302532.1635754/94‑1 (MQ=60)

                GACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGC                                                                               <  SRR4302532.4089848/94‑1 (MQ=60)

                 ACGTCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCA                                                                              <  SRR4302532.600969/94‑1 (MQ=60)

                 ACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCA                                                                              <  SRR4302532.600968/94‑1 (MQ=60)

                  CGGCATCTTCCTGATATTAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAT                                                                             <  SRR4302532.6106087/94‑1 (MQ=60)

                    GCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTT                                                                           >  SRR4302532.3025310/1‑94 (MQ=60)

                    GCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTT                                                                           >  SRR4302532.2534310/1‑94 (MQ=60)

                     CATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTC                                                                          >  SRR4302532.3296796/1‑94 (MQ=60)

                     CATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTC                                                                          >  SRR4302532.3588758/1‑94 (MQ=60)

                     CATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTC                                                                          >  SRR4302532.4353195/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         <  SRR4302532.2034448/94‑1 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         >  SRR4302532.4750937/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         >  SRR4302532.831340/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         >  SRR4302532.1257562/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         <  SRR4302532.3263609/94‑1 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         >  SRR4302532.5966173/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGGTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGGTGCCGAGCATTTCC                                                                         >  SRR4302532.1884690/1‑94 (MQ=60)

                       TCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCA                                                                        >  SRR4302532.3484698/1‑94 (MQ=60)

                        CTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAG                                                                       <  SRR4302532.187483/94‑1 (MQ=60)

                        CTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAG                                                                       <  SRR4302532.187482/94‑1 (MQ=60)

                         TTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGC                                                                      >  SRR4302532.1013034/1‑94 (MQ=60)

                         TTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGC                                                                      >  SRR4302532.3748071/1‑94 (MQ=60)

                          TCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCA                                                                     <  SRR4302532.2640954/94‑1 (MQ=60)

                            CTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGC                                                                   <  SRR4302532.3768754/94‑1 (MQ=60)

                            CTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGC                                                                   >  SRR4302532.5749878/1‑94 (MQ=60)

                             TGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCT                                                                  >  SRR4302532.1264597/1‑94 (MQ=60)

                              GATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTC                                                                 >  SRR4302532.4184877/1‑94 (MQ=60)

                               aaaaaaacgcgccccacttttgttcatcCCAGGGTTATCGACAACCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCT                                                                <  SRR4302532.4117132/66‑1 (MQ=60)

                               ATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCT                                                                >  SRR4302532.6245461/1‑94 (MQ=60)

                                TAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTT                                                               <  SRR4302532.3001893/94‑1 (MQ=60)

                                 AATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTC                                                              >  SRR4302532.4318719/1‑94 (MQ=60)

                                  ATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCC                                                             >  SRR4302532.1258045/1‑94 (MQ=60)

                                  ATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCC                                                             <  SRR4302532.6285781/94‑1 (MQ=60)

                                  ATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCC                                                             <  SRR4302532.664322/94‑1 (MQ=60)

                                   TAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCG                                                            <  SRR4302532.4233741/94‑1 (MQ=60)

                                    AACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTCCGGTTCGCTGCCGAGCATTTTCAGCATCTCTTCCGG                                                           >  SRR4302532.4428649/1‑94 (MQ=60)

                                     ACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGG                                                          <  SRR4302532.5291238/94‑1 (MQ=60)

                                     ACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGG                                                          <  SRR4302532.1434688/94‑1 (MQ=60)

                                     ACGCAGGCCCAGCTCGCTCATGCCAGGCTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGG                                                          <  SRR4302532.4046245/94‑1 (MQ=60)

                                     ACGCAGGCCCAGCTCACTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGG                                                          <  SRR4302532.5467652/94‑1 (MQ=60)

                                       GCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCG                                                        >  SRR4302532.2365347/1‑94 (MQ=60)

                                       GCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCG                                                        >  SRR4302532.2365348/1‑94 (MQ=60)

                                       GCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCG                                                        >  SRR4302532.728342/1‑94 (MQ=60)

                                          GGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTG                                                     >  SRR4302532.3909092/1‑94 (MQ=60)

                                            CCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCC                                                   >  SRR4302532.2365026/1‑94 (MQ=60)

                                            CCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCC                                                   >  SRR4302532.3228238/1‑94 (MQ=60)

                                            CCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCC                                                   >  SRR4302532.4808309/1‑94 (MQ=60)

                                             CCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCA                                                  <  SRR4302532.229049/94‑1 (MQ=60)

                                             CCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCA                                                  >  SRR4302532.1394505/1‑94 (MQ=60)

                                              CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAC                                                 >  SRR4302532.3657621/1‑94 (MQ=60)

                                              CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAC                                                 >  SRR4302532.196989/1‑94 (MQ=60)

                                              CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAC                                                 >  SRR4302532.3910217/1‑94 (MQ=60)

                                               AGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGGTCTTCCGGGCGGTTCCACT                                                >  SRR4302532.3942571/1‑94 (MQ=60)

                                               AGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACT                                                >  SRR4302532.1652028/1‑94 (MQ=60)

                                                GCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTC                                               <  SRR4302532.1759337/94‑1 (MQ=60)

                                                 CTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCG                                              >  SRR4302532.2920952/1‑94 (MQ=60)

                                                    GCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGT                                           <  SRR4302532.4746680/94‑1 (MQ=60)

                                                    GCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGT                                           <  SRR4302532.7359/94‑1 (MQ=60)

                                                     CTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTA                                          <  SRR4302532.1136206/94‑1 (MQ=60)

                                                     CTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTA                                          >  SRR4302532.5249450/1‑94 (MQ=60)

                                                      TCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGATCTTCCGGGCGGTGCCACTCGGGTAA                                         >  SRR4302532.4464701/1‑94 (MQ=60)

                                                       CATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAG                                        <  SRR4302532.5757901/94‑1 (MQ=60)

                                                        ATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGG                                       <  SRR4302532.51010/94‑1 (MQ=60)

                                                        ATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGG                                       <  SRR4302532.3544024/94‑1 (MQ=60)

                                                        ATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGGAAGG                                       >  SRR4302532.4615616/1‑94 (MQ=60)

                                                          GCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCT                                     >  SRR4302532.5209248/1‑94 (MQ=60)

                                                            CAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTT                                   >  SRR4302532.1422742/1‑94 (MQ=60)

                                                            CAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTT                                   >  SRR4302532.1051539/1‑94 (MQ=60)

                                                            CAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTT                                   >  SRR4302532.1040053/1‑94 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  <  SRR4302532.451035/94‑1 (MQ=60)

                                                              GGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCG                                 >  SRR4302532.5840208/1‑94 (MQ=60)

                                                              GGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCG                                 >  SRR4302532.974643/1‑94 (MQ=60)

                                                              GGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGCAAGGCTTTCG                                 >  SRR4302532.4152532/1‑94 (MQ=60)

                                                               GGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGA                                >  SRR4302532.4660121/1‑94 (MQ=60)

                                                                GTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAC                               >  SRR4302532.1200162/1‑94 (MQ=60)

                                                                 TTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACA                              <  SRR4302532.60906/94‑1 (MQ=60)

                                                                  TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACAT                             <  SRR4302532.3826689/94‑1 (MQ=60)

                                                                  TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGGAAGGCTTTAGACAT                             >  SRR4302532.578465/1‑94 (MQ=60)

                                                                   ATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATA                            >  SRR4302532.1348073/1‑94 (MQ=60)

                                                                    TCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAT                           <  SRR4302532.4711224/94‑1 (MQ=60)

                                                                     CGTCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGTGCGGTGCCACTCGGGTAAGGCTTTCGACATATT                          >  SRR4302532.5845927/1‑94 (MQ=60)

                                                                     CGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATT                          <  SRR4302532.6202168/94‑1 (MQ=60)

                                                                     CGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGGAAGGCTTTCGACATATT                          >  SRR4302532.1563924/1‑94 (MQ=60)

                                                                      GGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTG                         >  SRR4302532.5212830/1‑94 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGC                        >  SRR4302532.1158852/1‑94 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTACCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGC                        >  SRR4302532.465575/1‑94 (MQ=60)

                                                                        CAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCG                       <  SRR4302532.2446298/94‑1 (MQ=60)

                                                                        CAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCG                       >  SRR4302532.5266595/1‑94 (MQ=60)

                                                                        CAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCG                       <  SRR4302532.5046745/94‑1 (MQ=60)

                                                                        CAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGAGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCG                       <  SRR4302532.4265777/94‑1 (MQ=60)

                                                                         AAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTACGGCTTTCGACATATTGCGG                      >  SRR4302532.1979004/1‑94 (MQ=60)

                                                                          AACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGCCATATTGCGGG                     >  SRR4302532.1797351/1‑94 (MQ=60)

                                                                          AACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGG                     <  SRR4302532.3568955/94‑1 (MQ=60)

                                                                          AACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGG                     <  SRR4302532.794030/94‑1 (MQ=60)

                                                                          AACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGG                     <  SRR4302532.1491172/94‑1 (MQ=60)

                                                                           ACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGT                    <  SRR4302532.5172568/94‑1 (MQ=60)

                                                                           ACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGT                    <  SRR4302532.3888526/94‑1 (MQ=60)

                                                                           ACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGA                    >  SRR4302532.1449080/1‑94 (MQ=60)

                                                                            CCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTA                   <  SRR4302532.1092686/94‑1 (MQ=60)

                                                                             CACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTAT                  <  SRR4302532.6221511/94‑1 (MQ=60)

                                                                             CACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTAT                  <  SRR4302532.5120711/94‑1 (MQ=60)

                                                                              ACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATC                 >  SRR4302532.5925076/1‑94 (MQ=60)

                                                                              ACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATC                 >  SRR4302532.1000718/1‑94 (MQ=60)

                                                                               CTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCA                >  SRR4302532.2692141/1‑94 (MQ=60)

                                                                               CTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCA                >  SRR4302532.1747400/1‑94 (MQ=60)

                                                                               CTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGAGGGTATCA                >  SRR4302532.399229/1‑94 (MQ=60)

                                                                                TGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCAC               <  SRR4302532.5168971/94‑1 (MQ=60)

                                                                                  ATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGC             >  SRR4302532.1351655/1‑94 (MQ=60)

                                                                                   TTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCA            >  SRR4302532.5033640/1‑94 (MQ=60)

                                                                                   TTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCA            >  SRR4302532.19358/1‑94 (MQ=60)

                                                                                    TCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCAC           <  SRR4302532.436690/94‑1 (MQ=60)

                                                                                    TCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCAC           >  SRR4302532.1029260/1‑94 (MQ=60)

                                                                                     CTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACG          <  SRR4302532.463625/94‑1 (MQ=60)

                                                                                       GTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTC        <  SRR4302532.5053705/94‑1 (MQ=60)

                                                                                        TTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCG       >  SRR4302532.3006249/1‑94 (MQ=60)

                                                                                        TTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCG       <  SRR4302532.4374502/94‑1 (MQ=60)

                                                                                        TTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCG       <  SRR4302532.528049/94‑1 (MQ=60)

                                                                                        TTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCG       >  SRR4302532.441575/1‑94 (MQ=60)

                                                                                        TTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCG       <  SRR4302532.6096616/94‑1 (MQ=60)

                                                                                        TTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCG       <  SRR4302532.4213785/94‑1 (MQ=60)

                                                                                          TTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCG     >  SRR4302532.5010987/1‑94 (MQ=60)

                                                                                          TTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCG     <  SRR4302532.6024277/94‑1 (MQ=60)

                                                                                             TCGGTTCGCTGCCGAGCATGTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  <  SRR4302532.6093872/94‑1 (MQ=60)

                                                                                             ACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  <  SRR4302532.3514783/94‑1 (MQ=60)

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  >  NC_000913/4582957‑4583143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`