Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,583,050	G→A	Q446* (CAA→TAA)	hsdR ←	endonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,583,050	0	G	A	99.6%	1014.0 / NA	261	Q446* (CAA→TAA)	hsdR	endonuclease R
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (122/138); total (122/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCG  >  NC_000913/4582960‑4583140

  tCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGt                                                                                        <  2:4569166/93‑1 (MQ=255)

   cAACCGCGCGGAGGCTATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGtt                                                                                       <  1:5599988/93‑1 (MQ=255)

   cAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGtt                                                                                       <  2:6440899/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      <  1:28390/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      <  2:2769586/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      >  1:1974741/1‑93 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                      <  1:2453339/93‑1 (MQ=255)

      ccacgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCAGGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                    <  2:2628433/90‑1 (MQ=255)

      ccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                    <  1:4221785/93‑1 (MQ=255)

      ccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                    <  2:1278883/93‑1 (MQ=255)

           cgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCg                                                                               >  1:3486930/1‑93 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                              <  1:4288228/93‑1 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                              <  1:3100552/93‑1 (MQ=255)

              aCGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGc                                                                            <  1:1837821/93‑1 (MQ=255)

               cGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCa                                                                           <  1:4976630/93‑1 (MQ=255)

                 gCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                         <  2:6585914/93‑1 (MQ=255)

                 gCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                         <  2:4371281/93‑1 (MQ=255)

                  cATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAttt                                                                        >  1:3876227/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACTGTTCGCTGCCGAGCATTTcc                                                                      >  2:6495419/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                      >  2:4153491/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                      <  1:3678818/93‑1 (MQ=255)

                     cTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCca                                                                     >  1:4850743/1‑93 (MQ=255)

                      ttCCTGATAATAACGCAGGCCCAGCGCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcag                                                                    <  1:760042/93‑1 (MQ=255)

                        ccTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagca                                                                  >  1:1428111/1‑93 (MQ=255)

                         cTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagcag                                                                 <  1:1653491/93‑1 (MQ=255)

                          tGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCagcagc                                                                >  2:3890054/1‑93 (MQ=255)

                            ataataACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctc                                                              >  1:3207992/1‑93 (MQ=255)

                            ataataACGCAGGCCCAGCCCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctc                                                              <  2:2163255/93‑1 (MQ=255)

                              aataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCtt                                                            <  2:5027468/93‑1 (MQ=255)

                              aataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCtt                                                            >  2:4830226/1‑93 (MQ=255)

                               ataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTc                                                           <  2:1742894/93‑1 (MQ=255)

                                taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                          <  2:1695334/93‑1 (MQ=255)

                                taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                          <  2:579519/93‑1 (MQ=255)

                                taaCGCAGCCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                          <  2:6542429/93‑1 (MQ=255)

                                 aaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCg                                                         <  1:228557/93‑1 (MQ=255)

                                   cGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCggg                                                       <  2:723241/93‑1 (MQ=255)

                                    gCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGc                                                      >  2:1146008/1‑93 (MQ=255)

                                      cggCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCgg                                                    <  2:5435083/92‑1 (MQ=255)

                                      aGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGccg                                                    >  1:6409288/1‑91 (MQ=255)

                                      aGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCgg                                                    >  1:809563/1‑93 (MQ=255)

                                       ggCCCAGCTCGCTCATGCCAGGGCTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCCGCAGCTCTTCCGGGCGGt                                                   >  2:2410984/1‑93 (MQ=255)

                                        gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTg                                                  <  2:3627686/93‑1 (MQ=255)

                                         cccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGc                                                 >  2:3807738/1‑93 (MQ=255)

                                          ccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGcc                                                >  1:4636817/1‑93 (MQ=255)

                                           cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCa                                               <  1:2695495/93‑1 (MQ=255)

                                           cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCa                                               >  1:4466698/1‑93 (MQ=255)

                                            aGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAc                                              <  1:6624472/93‑1 (MQ=255)

                                            aGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAc                                              <  1:5793273/93‑1 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                             >  2:5881337/1‑93 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                             >  2:5330111/1‑93 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                           <  2:3945158/93‑1 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                           <  2:5177835/93‑1 (MQ=255)

                                                cgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCgg                                          <  2:3248371/93‑1 (MQ=255)

                                                 gctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCggg                                         <  1:5908292/93‑1 (MQ=255)

                                                  ctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGt                                        <  1:6599209/93‑1 (MQ=255)

                                                  ctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGt                                        <  1:5392893/93‑1 (MQ=255)

                                                   tcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTa                                       <  2:3451209/93‑1 (MQ=255)

                                                     aTGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAg                                     <  2:1501601/93‑1 (MQ=255)

                                                     aTGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAg                                     <  1:2549787/93‑1 (MQ=255)

                                                       gCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc                                   <  1:6178550/93‑1 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                  >  2:2586989/1‑93 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                  >  2:1409140/1‑93 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                                >  1:4541941/1‑93 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                                <  1:2608397/93‑1 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                                <  2:1383768/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                               <  2:590446/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                               >  2:598179/1‑93 (MQ=255)

                                                            ggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCg                              >  2:5588454/1‑93 (MQ=255)

                                                             gTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGa                             <  2:4025518/93‑1 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCCCTCGGGTAAGGCTTTCGAc                            >  1:54449/1‑93 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc                            >  2:405844/1‑93 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc                            >  1:1354836/1‑93 (MQ=255)

                                                                 tCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACata                         <  2:5003388/93‑1 (MQ=255)

                                                                 tCGCCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACata                         <  1:1010645/93‑1 (MQ=255)

                                                                  cGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACatat                        <  1:976052/93‑1 (MQ=255)

                                                                  cGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACatat                        <  2:2308063/93‑1 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                       >  2:1243562/1‑93 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                       <  2:21921/93‑1 (MQ=255)

                                                                    gCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTg                      >  2:4304745/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                     >  2:3059600/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCCGGGCAGGCTTTCGACATATTgg                     >  1:5215911/1‑92 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                    >  2:5707482/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                    >  2:5280583/1‑93 (MQ=255)

                                                                          cACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTa                >  2:2079283/1‑93 (MQ=255)

                                                                            cTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATc              <  1:2704497/93‑1 (MQ=255)

                                                                             tGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATca             <  2:1801122/93‑1 (MQ=255)

                                                                             tGATTCTGTTTTTACGGTGCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATca             <  1:1104273/93‑1 (MQ=255)

                                                                              gATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcac            >  2:5849988/1‑93 (MQ=255)

                                                                              gATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcac            >  2:4671731/1‑93 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg           >  2:3697138/1‑93 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGATCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg           >  1:2981640/1‑93 (MQ=255)

                                                                                ttCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGTCATATTGCGGGTATcacgc          <  1:6458176/93‑1 (MQ=255)

                                                                                ttCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgc          >  1:472885/1‑93 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca         >  1:687656/1‑93 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca         <  2:2457989/93‑1 (MQ=255)

                                                                                   tGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcacg       <  2:5356473/93‑1 (MQ=255)

                                                                                   tGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcacg       <  1:6055698/93‑1 (MQ=255)

                                                                                    gTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGt      <  2:2017206/93‑1 (MQ=255)

                                                                                       tttACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcgc   <  2:4902023/93‑1 (MQ=255)

                                                                                        ttGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcgcg  <  1:2091172/93‑1 (MQ=255)

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCG  >  NC_000913/4582960‑4583140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  >  NC_000913/4582957‑4583143

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTA                                                                                               <  SRR4302547.5050011/94‑1 (MQ=60)

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTA                                                                                               <  SRR4302547.823014/94‑1 (MQ=60)

 CTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTAC                                                                                              >  SRR4302547.2019112/1‑94 (MQ=60)

  TTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACG                                                                                             >  SRR4302547.712599/1‑94 (MQ=60)

  TTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACG                                                                                             <  SRR4302547.4506323/94‑1 (MQ=60)

  TTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACG                                                                                             >  SRR4302547.2880564/1‑94 (MQ=60)

    TTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGT                                                                                           >  SRR4302547.1148651/1‑94 (MQ=60)

      CAACCGCGCGGAGGCTATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTC                                                                                         <  SRR4302547.5715499/94‑1 (MQ=60)

      CAACCGCGCGGACGTCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTC                                                                                         <  SRR4302547.2711090/94‑1 (MQ=60)

      CAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTC                                                                                         <  SRR4302547.413394/94‑1 (MQ=60)

      CAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTC                                                                                         >  SRR4302547.2013957/1‑94 (MQ=60)

       AACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCG                                                                                        <  SRR4302547.4885975/94‑1 (MQ=60)

       AACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCG                                                                                        <  SRR4302547.2502918/94‑1 (MQ=60)

       AACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCG                                                                                        <  SRR4302547.28906/94‑1 (MQ=60)

        ACCGCGAGGACGGCATCTTCCTGATAATAACGCAGGCCCCGCCCGCTCATGCCAGGTTTATCGGCAAACCACTGATTATGTTTTTACGGTTCGC                                                                                       <  SRR4302547.2394155/94‑1 (MQ=60)

         CCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCT                                                                                      <  SRR4302547.5402459/94‑1 (MQ=60)

         CCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCT                                                                                      <  SRR4302547.4308012/94‑1 (MQ=60)

            CGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCC                                                                                   <  SRR4302547.6300377/94‑1 (MQ=60)

            CGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCC                                                                                   <  SRR4302547.4589859/94‑1 (MQ=60)

             GCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCG                                                                                  >  SRR4302547.3558072/1‑94 (MQ=60)

             GCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCG                                                                                  <  SRR4302547.2322247/94‑1 (MQ=60)

              TGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGANTCTGTTTTTACGGNTNGCTGCCGA                                                                                 >  SRR4302547.1953609/1‑94 (MQ=60)

              CGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGA                                                                                 >  SRR4302547.3036234/1‑94 (MQ=60)

              CGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGA                                                                                 >  SRR4302547.1687199/1‑94 (MQ=60)

               GGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                <  SRR4302547.3161962/94‑1 (MQ=60)

               GGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                <  SRR4302547.4376363/94‑1 (MQ=60)

               GGACGCTATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAG                                                                                <  SRR4302547.5830730/94‑1 (MQ=60)

                GACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGC                                                                               >  SRR4302547.3498592/1‑94 (MQ=60)

                GACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGC                                                                               <  SRR4302547.3678236/94‑1 (MQ=60)

                 ACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCA                                                                              <  SRR4302547.1874146/94‑1 (MQ=60)

                  CGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAT                                                                             <  SRR4302547.1977674/94‑1 (MQ=60)

                  CGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAT                                                                             <  SRR4302547.5079576/94‑1 (MQ=60)

                  CGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAT                                                                             <  SRR4302547.4751719/94‑1 (MQ=60)

                    GCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTT                                                                           >  SRR4302547.4423158/1‑94 (MQ=60)

                    GCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTT                                                                           >  SRR4302547.3955098/1‑94 (MQ=60)

                     CATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTC                                                                          >  SRR4302547.6246627/1‑94 (MQ=60)

                     CATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTC                                                                          >  SRR4302547.2668465/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         >  SRR4302547.6442671/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCC                                                                         >  SRR4302547.3944744/1‑94 (MQ=60)

                      ATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTAGCTGCCGAGCATTTCC                                                                         >  SRR4302547.3848359/1‑94 (MQ=60)

                       TCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCA                                                                        <  SRR4302547.3753926/94‑1 (MQ=60)

                       TCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCA                                                                        >  SRR4302547.4951259/1‑94 (MQ=60)

                       ACTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCA                                                                        <  SRR4302547.6120269/94‑1 (MQ=60)

                        CTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAG                                                                       >  SRR4302547.5312407/1‑94 (MQ=60)

                         TTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGC                                                                      >  SRR4302547.823151/1‑94 (MQ=60)

                         TTCCTGATAATAACGCAGGCCCAGCGCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGC                                                                      <  SRR4302547.774370/94‑1 (MQ=60)

                          TCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCA                                                                     >  SRR4302547.1456677/1‑94 (MQ=60)

                            CTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGC                                                                   <  SRR4302547.1686111/94‑1 (MQ=60)

                            CTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGC                                                                   >  SRR4302547.736446/1‑94 (MQ=60)

                             TGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCT                                                                  <  SRR4302547.580764/94‑1 (MQ=60)

                              GATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTC                                                                 >  SRR4302547.3271984/1‑94 (MQ=60)

                               ATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCT                                                                <  SRR4302547.6764630/94‑1 (MQ=60)

                                  ATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCC                                                             <  SRR4302547.549291/94‑1 (MQ=60)

                                  ATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCA                                                             <  SRR4302547.454291/94‑1 (MQ=60)

                                    AACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGG                                                           <  SRR4302547.232845/94‑1 (MQ=60)

                                        CAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGG                                                       >  SRR4302547.6592301/1‑94 (MQ=60)

                                        CAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGG                                                       >  SRR4302547.824903/1‑94 (MQ=60)

                                        CAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGG                                                       <  SRR4302547.3232752/94‑1 (MQ=60)

                                        CAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCCG                                                       >  SRR4302547.6542948/1‑94 (MQ=60)

                                          GGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTG                                                     <  SRR4302547.5279847/94‑1 (MQ=60)

                                          GGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTG                                                     <  SRR4302547.4658677/94‑1 (MQ=60)

                                            CCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCC                                                   >  SRR4302547.4732864/1‑94 (MQ=60)

                                            CCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCC                                                   >  SRR4302547.5403578/1‑94 (MQ=60)

                                             CCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCA                                                  >  SRR4302547.4558994/1‑94 (MQ=60)

                                              CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAC                                                 >  SRR4302547.50303/1‑94 (MQ=60)

                                              CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAC                                                 <  SRR4302547.2749609/94‑1 (MQ=60)

                                               cgcgCGCTCAGGCCAGGGTTATCGGCAAGCCACTGATTCTGTGTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACT                                                <  SRR4302547.4839920/90‑1 (MQ=60)

                                               AGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACT                                                <  SRR4302547.5913110/94‑1 (MQ=60)

                                               AGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACT                                                <  SRR4302547.6762370/94‑1 (MQ=60)

                                                GCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTC                                               <  SRR4302547.1415340/94‑1 (MQ=60)

                                                 CTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCG                                              >  SRR4302547.2765020/1‑94 (MQ=60)

                                                 CTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGATGCCACTCG                                              >  SRR4302547.6586275/1‑94 (MQ=60)

                                                  TCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGG                                             <  SRR4302547.2611709/94‑1 (MQ=60)

                                                   CGCTCATGCCAGGTTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGG                                            <  SRR4302547.3194102/94‑1 (MQ=60)

                                                   CGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGG                                            <  SRR4302547.5909769/94‑1 (MQ=60)

                                                    GCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGT                                           <  SRR4302547.6030647/94‑1 (MQ=60)

                                                     CTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTA                                          <  SRR4302547.5504289/94‑1 (MQ=60)

                                                     CTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTA                                          <  SRR4302547.6736581/94‑1 (MQ=60)

                                                     ATCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTA                                          >  SRR4302547.6771141/1‑94 (MQ=60)

                                                      ccgTGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCCCTCGGGTAA                                         <  SRR4302547.5506511/91‑1 (MQ=60)

                                                      TCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAA                                         <  SRR4302547.2644140/94‑1 (MQ=60)

                                                      CCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAA                                         <  SRR4302547.4116358/94‑1 (MQ=60)

                                                        ATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGG                                       <  SRR4302547.2601278/94‑1 (MQ=60)

                                                        ATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGG                                       >  SRR4302547.2750267/1‑94 (MQ=60)

                                                          GCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCT                                     <  SRR4302547.6306793/94‑1 (MQ=60)

                                                          GCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAACAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCT                                     <  SRR4302547.3522474/94‑1 (MQ=60)

                                                           CCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTT                                    >  SRR4302547.2121777/1‑94 (MQ=60)

                                                            CAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTT                                   >  SRR4302547.4635838/1‑94 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  >  SRR4302547.1160556/1‑94 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  <  SRR4302547.674259/94‑1 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  >  SRR4302547.6142912/1‑94 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  <  SRR4302547.2660970/94‑1 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  <  SRR4302547.1857333/94‑1 (MQ=60)

                                                             AGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTC                                  >  SRR4302547.1495199/1‑94 (MQ=60)

                                                              GGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCG                                 >  SRR4302547.5560771/1‑94 (MQ=60)

                                                              GGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCG                                 <  SRR4302547.5574986/94‑1 (MQ=60)

                                                                GTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCCCTCGGGTAAGGCTTTCGAC                               >  SRR4302547.55473/1‑94 (MQ=60)

                                                                GTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAC                               >  SRR4302547.1382030/1‑94 (MQ=60)

                                                                  TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACAT                             <  SRR4302547.1475298/94‑1 (MQ=60)

                                                                   ATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATA                            <  SRR4302547.4350225/94‑1 (MQ=60)

                                                                    TCGCCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAT                           <  SRR4302547.1030396/94‑1 (MQ=60)

                                                                    TCGCCAAACCACTGATTCTGTTTCTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAT                           <  SRR4302547.2936583/94‑1 (MQ=60)

                                                                     CGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATT                          <  SRR4302547.995036/94‑1 (MQ=60)

                                                                     CGGCAAACCACTGATTCTGTTTTCACGGTGCGCTGCCGAGCATTCCCAGCAGCTCTTCCGGGCGGTGCCTCTCGGGTAGGGCTTTCGGCATATT                          <  SRR4302547.4492752/94‑1 (MQ=60)

                                                                      GGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTG                         <  SRR4302547.4901170/94‑1 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGC                        >  SRR4302547.1406297/1‑94 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGC                        <  SRR4302547.4738858/94‑1 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGC                        <  SRR4302547.2533287/94‑1 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGC                        <  SRR4302547.6694190/94‑1 (MQ=60)

                                                                       GCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCCGGGCAGGCTTTCGACATATTGG                        >  SRR4302547.5323272/1‑94 (MQ=60)

                                                                        CAGACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCGGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTGTCGACATATTGCG                       <  SRR4302547.5927726/94‑1 (MQ=60)

                                                                         AAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGG                      <  SRR4302547.928822/94‑1 (MQ=60)

                                                                         AAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGG                      <  SRR4302547.3083191/94‑1 (MQ=60)

                                                                          ACCCACTGATTCTGTTTTTCCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCGGGGCGGTGCCACTCGGGagagctgctggaaatgctcggca                     <  SRR4302547.1807565/94‑24 (MQ=60)

                                                                          AACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGG                     >  SRR4302547.6602097/1‑94 (MQ=60)

                                                                            CCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTA                   >  SRR4302547.4609181/1‑94 (MQ=60)

                                                                              ACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATC                 <  SRR4302547.833935/94‑1 (MQ=60)

                                                                               CTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCA                <  SRR4302547.2758806/94‑1 (MQ=60)

                                                                                TGATTCTGTTTTTACGGTGCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCAC               <  SRR4302547.1126482/94‑1 (MQ=60)

                                                                                 GATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGATCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACG              >  SRR4302547.3040978/1‑94 (MQ=60)

                                                                                  ATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGC             >  SRR4302547.481571/1‑94 (MQ=60)

                                                                                   TTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGTCATATTGCGGGTATCACGCA            <  SRR4302547.6592677/94‑1 (MQ=60)

                                                                                   TTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCA            >  SRR4302547.700348/1‑94 (MQ=60)

                                                                                    TCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCAC           <  SRR4302547.1033993/94‑1 (MQ=60)

                                                                                      TGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGT         >  SRR4302547.22314/1‑94 (MQ=60)

                                                                                      TGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGT         <  SRR4302547.6181314/94‑1 (MQ=60)

                                                                                       GTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTC        <  SRR4302547.2476264/94‑1 (MQ=60)

                                                                                           TTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGA    <  SRR4302547.2132854/94‑1 (MQ=60)

                                                                                           TTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGA    <  SRR4302547.1606060/94‑1 (MQ=60)

                                                                                           TTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGA    >  SRR4302547.5511089/1‑94 (MQ=60)

                                                                                             ACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  <  SRR4302547.3387266/94‑1 (MQ=60)

CCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATA  >  NC_000913/4582957‑4583143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`