Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,324,623 T→C G618G (GGT→GGC)  yciQ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,324,6230TC82.5% 456.8 / 92.5 200G618G (GGT→GGC) yciQhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/22);  new base C (78/87);  total (91/109)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 221 total aligned reads displayed.

ATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGT  >  NC_000913/1324531‑1324714
                                                                                            |                                                                                           
aTTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCggtggtggt                                                                                             <  1:4842946/93‑1 (MQ=255)
  tGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGgcgc                                                                                           <  1:5061616/93‑1 (MQ=255)
   gCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCt                                                                                          <  1:4519411/93‑1 (MQ=255)
     ccGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTgg                                                                                        <  1:637242/93‑1 (MQ=255)
        tAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTggcgg                                                                                     <  2:4153243/93‑1 (MQ=255)
        tAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTggcgg                                                                                     >  1:1022413/1‑93 (MQ=255)
         aaCGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTggcggc                                                                                    <  1:3378939/93‑1 (MQ=255)
             gCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGgcggcggcg                                                                                >  2:6509733/1‑93 (MQ=255)
              cGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGgcgc                                                                               <  1:548989/93‑1 (MQ=255)
                ttttATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCgg                                                                             >  1:1210042/1‑93 (MQ=255)
                ttttATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCgg                                                                             >  2:319818/1‑93 (MQ=255)
                ttttATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCgg                                                                             >  2:4943354/1‑93 (MQ=255)
                 tttATTCTCATTACAGACGCACCTAAGTTGCGGGGCTAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCggg                                                                            <  2:5231218/93‑1 (MQ=255)
                 tttATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCggg                                                                            >  1:914301/1‑93 (MQ=255)
                 tttATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCggg                                                                            <  1:6567190/93‑1 (MQ=255)
                  ttATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGt                                                                           <  1:2896242/93‑1 (MQ=255)
                   tATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTg                                                                          <  1:2963566/93‑1 (MQ=255)
                   tATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTg                                                                          <  2:2762759/93‑1 (MQ=255)
                    aTTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTgg                                                                         <  2:267549/93‑1 (MQ=255)
                     ttCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGc                                                                        >  2:2004517/1‑93 (MQ=255)
                     ttCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGc                                                                        <  2:6284976/93‑1 (MQ=255)
                     ttCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGc                                                                        <  2:3154260/93‑1 (MQ=255)
                       ctcATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCgg                                                                      >  1:3015421/1‑93 (MQ=255)
                       ctcATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCgg                                                                      >  2:5806703/1‑93 (MQ=255)
                       ctcATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCgg                                                                      >  1:3846511/1‑93 (MQ=255)
                        tcATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCggg                                                                     >  1:3463001/1‑92 (MQ=255)
                         cATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAg                                                                    >  1:4870155/1‑93 (MQ=255)
                         cATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAg                                                                    <  1:2337083/93‑1 (MQ=255)
                          aTTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAgg                                                                   <  2:5908092/93‑1 (MQ=255)
                           ttACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGc                                                                  >  1:4258442/1‑93 (MQ=255)
                           ttACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGc                                                                  <  2:2879809/93‑1 (MQ=255)
                           ttACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGc                                                                  >  1:2736023/1‑93 (MQ=255)
                           ttACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGGGGCGGAggg                                                                  >  2:4596274/1‑92 (MQ=255)
                             aCAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCgg                                                                <  1:1628379/93‑1 (MQ=255)
                             aCAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCgg                                                                <  2:4686417/93‑1 (MQ=255)
                             aCAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCgg                                                                <  1:988962/93‑1 (MQ=255)
                              cAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCggg                                                               >  1:5994488/1‑92 (MQ=255)
                               aGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCggtg                                                              >  1:4732662/1‑93 (MQ=255)
                                gACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCggtgg                                                             >  1:4415596/1‑93 (MQ=255)
                                gACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCggtgg                                                             >  1:2136489/1‑93 (MQ=255)
                                gACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCggtgg                                                             >  2:2149929/1‑93 (MQ=255)
                                 aCGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGAGGGTGGCGGAGGCggtggt                                                            <  1:4128931/93‑1 (MQ=255)
                                   gCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGtggtgg                                                          <  2:1375731/93‑1 (MQ=255)
                                   gCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGtggtgg                                                          <  1:4324433/93‑1 (MQ=255)
                                    cACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGtgggggg                                                         >  1:4954641/1‑92 (MQ=255)
                                    cACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGGGGGGGc                                                         >  2:4394950/1‑93 (MQ=255)
                                     aCCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCt                                                        <  1:1236090/93‑1 (MQ=255)
                                      ccTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGAGGCGGAGGCGGTGGTGGCTg                                                       >  2:5489061/1‑93 (MQ=255)
                                       cTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGGGGTGGCTgg                                                      >  2:3829858/1‑93 (MQ=255)
                                        tAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGGGGGGGCTGGt                                                     >  2:3571083/1‑93 (MQ=255)
                                         aaGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTa                                                    <  1:2454239/93‑1 (MQ=255)
                                              gCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGGGGGGGCTGGTAATTaa                                               >  2:3540091/1‑93 (MQ=255)
                                               cGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAg                                              <  2:1550010/93‑1 (MQ=255)
                                               cGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAg                                              <  2:881338/93‑1 (MQ=255)
                                               cGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAg                                              <  1:4573921/93‑1 (MQ=255)
                                                ggggCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGc                                             <  1:3708928/93‑1 (MQ=255)
                                                 gggCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCt                                            >  1:5836491/1‑93 (MQ=255)
                                                   gCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGa                                          <  2:4758614/93‑1 (MQ=255)
                                                   gCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGa                                          >  2:1312529/1‑93 (MQ=255)
                                                   gCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGa                                          <  1:6087869/93‑1 (MQ=255)
                                                    ccAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGAt                                         <  2:6065864/93‑1 (MQ=255)
                                                     cAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATg                                        >  1:1255129/1‑93 (MQ=255)
                                                      aaTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGt                                       >  1:2194088/1‑93 (MQ=255)
                                                      aaTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGt                                       >  1:787559/1‑93 (MQ=255)
                                                      aaTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGt                                       <  2:2413632/93‑1 (MQ=255)
                                                      aaTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGt                                       >  1:5347540/1‑93 (MQ=255)
                                                       aTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGtt                                      <  2:2264598/93‑1 (MQ=255)
                                                       aTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGtt                                      <  2:6190688/93‑1 (MQ=255)
                                                       aTACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGtt                                      >  1:3557613/1‑93 (MQ=255)
                                                        tACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTa                                     >  1:4113684/1‑93 (MQ=255)
                                                         aCCAGTTTGTTGGGGCGAAGTTATTTCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTaa                                    >  1:2697563/1‑93 (MQ=255)
                                                          ccAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAAt                                   <  1:4966881/93‑1 (MQ=255)
                                                          ccAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAAt                                   >  1:4046142/1‑93 (MQ=255)
                                                             gTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGa                                >  1:1363376/1‑93 (MQ=255)
                                                              tttGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAAATAAGCTGATGTTAATCGaa                               >  1:2522899/1‑93 (MQ=255)
                                                              tttGTTGGGGAGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGaa                               <  1:1602167/93‑1 (MQ=255)
                                                               ttgttgGGGCGAAGTTATTCCGGTGGTGGCGATGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGaaa                              >  1:4180416/1‑93 (MQ=255)
                                                                 gttgGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACa                            <  2:5353129/93‑1 (MQ=255)
                                                                 gttgGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACa                            >  1:3874612/1‑93 (MQ=255)
                                                                 gttgGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACa                            <  2:3058306/93‑1 (MQ=255)
                                                                  ttgGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACaa                           <  1:6531137/93‑1 (MQ=255)
                                                                  ttgGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACaa                           <  1:1752976/93‑1 (MQ=255)
                                                                       gCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCttt                      >  2:553857/1‑93 (MQ=255)
                                                                       gCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCttt                      <  2:724465/93‑1 (MQ=255)
                                                                        cGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTa                     <  2:2623416/93‑1 (MQ=255)
                                                                         gAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTaa                    <  1:1970817/93‑1 (MQ=255)
                                                                          aaGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAAc                   >  1:104492/1‑93 (MQ=255)
                                                                           aGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAAcc                  <  2:2781453/93‑1 (MQ=255)
                                                                             ttATTGCGGTGGTGGCGGTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCAt                <  2:1073292/93‑1 (MQ=255)
                                                                             ttATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCAt                >  1:1917436/1‑93 (MQ=255)
                                                                             ttATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCAt                >  2:3367238/1‑93 (MQ=255)
                                                                              tATTCCGGTGGTGGCGATGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATa               <  2:1498733/93‑1 (MQ=255)
                                                                               aTTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATaa              <  1:3282741/93‑1 (MQ=255)
                                                                                  ccGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGa           >  2:5574342/1‑93 (MQ=255)
                                                                                  ccGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGa           <  2:1752646/93‑1 (MQ=255)
                                                                                  ccGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGa           <  1:757401/93‑1 (MQ=255)
                                                                                     gtggtggtgCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAg        >  1:5297597/1‑93 (MQ=255)
                                                                                     gtggtggtgCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAg        <  2:6610430/93‑1 (MQ=255)
                                                                                      tggtggcgCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAgt       <  1:3078970/93‑1 (MQ=255)
                                                                                       ggtggtgCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAgtg      <  2:1841516/93‑1 (MQ=255)
                                                                                            |                                                                                           
ATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGT  >  NC_000913/1324531‑1324714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTA  >  NC_000913/1324531‑1324715
                                                                                            |                                                                                            
ATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTG                                                                                             <  SRR4302547.4943315/94‑1 (MQ=60)
 TTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGC                                                                                            >  SRR4302547.1643076/1‑94 (MQ=60)
  TGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCT                                                                                           <  SRR4302547.5166069/94‑1 (MQ=60)
  TGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCT                                                                                           <  SRR4302547.4911706/94‑1 (MQ=60)
   GCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTG                                                                                          <  SRR4302547.5101059/94‑1 (MQ=60)
   GCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTG                                                                                          >  SRR4302547.2504845/1‑94 (MQ=60)
   GCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTG                                                                                          <  SRR4302547.4206048/94‑1 (MQ=60)
   GCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTG                                                                                          <  SRR4302547.4612776/94‑1 (MQ=60)
     CCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGC                                                                                        <  SRR4302547.649008/94‑1 (MQ=60)
     CCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGC                                                                                        >  SRR4302547.4708830/1‑94 (MQ=60)
       GTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGG                                                                                      >  SRR4302547.1042466/1‑94 (MQ=60)
        TAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGC                                                                                     <  SRR4302547.6298381/94‑1 (MQ=60)
         AACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCG                                                                                    <  SRR4302547.2137567/94‑1 (MQ=60)
         AACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCG                                                                                    <  SRR4302547.3447602/94‑1 (MQ=60)
         AACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCG                                                                                    <  SRR4302547.3554434/94‑1 (MQ=60)
           CGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGC                                                                                  >  SRR4302547.2547489/1‑94 (MQ=60)
              CGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCG                                                                               <  SRR4302547.558900/94‑1 (MQ=60)
              CGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCG                                                                               >  SRR4302547.619218/1‑94 (MQ=60)
              CGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGCCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCG                                                                               <  SRR4302547.1688238/94‑1 (MQ=60)
               GTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGG                                                                              >  SRR4302547.1264371/1‑94 (MQ=60)
               GTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGG                                                                              >  SRR4302547.1234460/1‑94 (MQ=60)
                TTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGG                                                                             >  SRR4302547.931939/1‑94 (MQ=60)
                 TTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGT                                                                            >  SRR4302547.3326794/1‑94 (MQ=60)
                 TTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGT                                                                            <  SRR4302547.6703920/94‑1 (MQ=60)
                  TTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTG                                                                           <  SRR4302547.348947/94‑1 (MQ=60)
                  TTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTG                                                                           <  SRR4302547.2953998/94‑1 (MQ=60)
                  TTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTG                                                                           <  SRR4302547.12826/94‑1 (MQ=60)
                   TATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGG                                                                          <  SRR4302547.3022548/94‑1 (MQ=60)
                    ATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGG                                                                         >  SRR4302547.303057/1‑94 (MQ=60)
                      TCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGG                                                                       >  SRR4302547.3924823/1‑94 (MQ=60)
                      TCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGG                                                                       >  SRR4302547.503108/1‑94 (MQ=60)
                      TCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGG                                                                       <  SRR4302547.1242784/94‑1 (MQ=60)
                      TCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGG                                                                       >  SRR4302547.3075387/1‑94 (MQ=60)
                       CTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGG                                                                      >  SRR4302547.3533618/1‑94 (MQ=60)
                        TCATTACAGACGCACCTAAGTTGCGGGGCCAATACCGGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAG                                                                     >  SRR4302547.2206718/1‑94 (MQ=60)
                        TCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAG                                                                     >  SRR4302547.4971075/1‑94 (MQ=60)
                         CATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGG                                                                    <  SRR4302547.2384459/94‑1 (MQ=60)
                          ATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGC                                                                   >  SRR4302547.4345583/1‑94 (MQ=60)
                          ATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGC                                                                   >  SRR4302547.2790933/1‑94 (MQ=60)
                           TTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCG                                                                  <  SRR4302547.4086276/94‑1 (MQ=60)
                             ACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGT                                                                <  SRR4302547.3848740/94‑1 (MQ=60)
                             ACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGT                                                                <  SRR4302547.2363341/94‑1 (MQ=60)
                             ACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGT                                                                <  SRR4302547.1008216/94‑1 (MQ=60)
                             ACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGT                                                                <  SRR4302547.1660591/94‑1 (MQ=60)
                             ACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGG                                                                >  SRR4302547.6118880/1‑94 (MQ=60)
                              CAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTG                                                               >  SRR4302547.4830754/1‑94 (MQ=60)
                               AGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGG                                                              <  SRR4302547.5268156/94‑1 (MQ=60)
                               AGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGG                                                              >  SRR4302547.4506688/1‑94 (MQ=60)
                               AGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGG                                                              >  SRR4302547.2179047/1‑94 (MQ=60)
                                 ACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTG                                                            <  SRR4302547.24143/94‑1 (MQ=60)
                                 ACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGGGGTG                                                            >  SRR4302547.4169184/1‑94 (MQ=60)
                                 ACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGAGGGTGGCGGAGGCGGTGGTG                                                            <  SRR4302547.4212916/94‑1 (MQ=60)
                                  CGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGG                                                           <  SRR4302547.6700563/94‑1 (MQ=60)
                                  CGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGG                                                           >  SRR4302547.4225740/1‑94 (MQ=60)
                                  CGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGG                                                           >  SRR4302547.6538670/1‑94 (MQ=60)
                                   GCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGgggg                                                          >  SRR4302547.5057199/1‑90 (MQ=60)
                                   GCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGC                                                          <  SRR4302547.4413454/94‑1 (MQ=60)
                                    CACCTAATTTACGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTTCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCT                                                         <  SRR4302547.5453879/94‑1 (MQ=60)
                                    CACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCT                                                         <  SRR4302547.5068883/94‑1 (MQ=60)
                                     ACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTG                                                        <  SRR4302547.1261031/94‑1 (MQ=60)
                                     ACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTG                                                        <  SRR4302547.5965764/94‑1 (MQ=60)
                                     ACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTG                                                        <  SRR4302547.4287060/94‑1 (MQ=60)
                                       CTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGT                                                      >  SRR4302547.439690/1‑94 (MQ=60)
                                       CTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGT                                                      >  SRR4302547.109167/1‑94 (MQ=60)
                                        TAAGTTGCGGGGCCAATATCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTA                                                     >  SRR4302547.1482937/1‑94 (MQ=60)
                                         AAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAA                                                    <  SRR4302547.2503839/94‑1 (MQ=60)
                                          AGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAAT                                                   <  SRR4302547.3738741/94‑1 (MQ=60)
                                              GCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAG                                               <  SRR4302547.3044240/94‑1 (MQ=60)
                                               CGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGC                                              >  SRR4302547.1833156/1‑94 (MQ=60)
                                               CGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGC                                              <  SRR4302547.4668566/94‑1 (MQ=60)
                                                GGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCT                                             <  SRR4302547.3784638/94‑1 (MQ=60)
                                                GGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCT                                             >  SRR4302547.5957249/1‑94 (MQ=60)
                                                GGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCT                                             <  SRR4302547.4951787/94‑1 (MQ=60)
                                                 GGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTG                                            <  SRR4302547.6091216/94‑1 (MQ=60)
                                                   GCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGAT                                          <  SRR4302547.6214157/94‑1 (MQ=60)
                                                    CCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATG                                         >  SRR4302547.5136711/1‑94 (MQ=60)
                                                    CCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATG                                         >  SRR4302547.1280439/1‑94 (MQ=60)
                                                     CAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGCTGT                                        >  SRR4302547.4639165/1‑94 (MQ=60)
                                                     CAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGT                                        >  SRR4302547.2238146/1‑94 (MQ=60)
                                                     CAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGT                                        >  SRR4302547.802468/1‑94 (MQ=60)
                                                     CAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGT                                        >  SRR4302547.5457797/1‑94 (MQ=60)
                                                     CAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGT                                        <  SRR4302547.5597208/94‑1 (MQ=60)
                                                      AATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTT                                       >  SRR4302547.3630219/1‑94 (MQ=60)
                                                       ATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTA                                      >  SRR4302547.4437031/1‑94 (MQ=60)
                                                       ATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTA                                      >  SRR4302547.4197289/1‑94 (MQ=60)
                                                       ATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTA                                      >  SRR4302547.1360933/1‑94 (MQ=60)
                                                        TACCAGTTTGTTGGGGCGAAGTTATTTCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAA                                     >  SRR4302547.2751714/1‑94 (MQ=60)
                                                        TACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAA                                     >  SRR4302547.5869758/1‑94 (MQ=60)
                                                         ACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAAT                                    >  SRR4302547.4128299/1‑94 (MQ=60)
                                                          CCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATC                                   <  SRR4302547.1976465/94‑1 (MQ=60)
                                                          CCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATC                                   <  SRR4302547.5069662/94‑1 (MQ=60)
                                                           CAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCG                                  >  SRR4302547.762100/1‑94 (MQ=60)
                                                            AGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGA                                 >  SRR4302547.1390732/1‑94 (MQ=60)
                                                             GTTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAAATAAGCTGATGTTAATCGAA                                >  SRR4302547.2573881/1‑94 (MQ=60)
                                                              TTTGTTGGGGCGAAGTTATTCCGGTGGTGGCGATGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAA                               >  SRR4302547.4265609/1‑94 (MQ=60)
                                                              TTTGTTGGGGAGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAA                               <  SRR4302547.1633943/94‑1 (MQ=60)
                                                               TTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAAC                              <  SRR4302547.3937495/94‑1 (MQ=60)
                                                                TGTTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACA                             >  SRR4302547.3953455/1‑94 (MQ=60)
                                                                  TTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAG                           <  SRR4302547.6667065/94‑1 (MQ=60)
                                                                  TTGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAG                           <  SRR4302547.1787633/94‑1 (MQ=60)
                                                                   TGGGGCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGC                          <  SRR4302547.810455/94‑1 (MQ=60)
                                                                       GCGAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTA                      >  SRR4302547.3100545/1‑94 (MQ=60)
                                                                         GAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAAC                    <  SRR4302547.2009956/94‑1 (MQ=60)
                                                                         GAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAAC                    >  SRR4302547.949925/1‑94 (MQ=60)
                                                                         GAAGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAAC                    >  SRR4302547.106399/1‑94 (MQ=60)
                                                                           AGTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCA                  >  SRR4302547.338511/1‑94 (MQ=60)
                                                                            GTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCAT                 <  SRR4302547.4117863/94‑1 (MQ=60)
                                                                            GTTATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCAT                 >  SRR4302547.1955406/1‑94 (MQ=60)
                                                                              TATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAA               <  SRR4302547.2589741/94‑1 (MQ=60)
                                                                               ATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAA              <  SRR4302547.3348559/94‑1 (MQ=60)
                                                                               ATTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAA              >  SRR4302547.4398321/1‑94 (MQ=60)
                                                                                TTCCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAG             <  SRR4302547.4923957/94‑1 (MQ=60)
                                                                                  CCGGTGGTGGNCCTGGCGGCGGCGNGNGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAT           <  SRR4302547.2908626/94‑1 (MQ=60)
                                                                                  CCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGTCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAT           <  SRR4302547.2977404/94‑1 (MQ=60)
                                                                                  CCGGTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGAT           <  SRR4302547.771680/94‑1 (MQ=60)
                                                                                    GGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAG         >  SRR4302547.5406892/1‑94 (MQ=60)
                                                                                     GTGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGT        <  SRR4302547.1994347/94‑1 (MQ=60)
                                                                                      TGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTG       <  SRR4302547.3140117/94‑1 (MQ=60)
                                                                                      TGGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTG       <  SRR4302547.4547291/94‑1 (MQ=60)
                                                                                       GGTGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGT      >  SRR4302547.1052789/1‑94 (MQ=60)
                                                                                         TGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAG    <  SRR4302547.747933/94‑1 (MQ=60)
                                                                                         TGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAG    <  SRR4302547.3529554/94‑1 (MQ=60)
                                                                                         TGGCGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAG    <  SRR4302547.799502/94‑1 (MQ=60)
                                                                                           GTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTA  <  SRR4302547.2763287/94‑1 (MQ=60)
                                                                                            |                                                                                            
ATTGCCCGTAACGGCGTTTTATTCTCATTACAGACGCACCTAAGTTGCGGGGCCAATACCAGTTTGTTGGGGCGAAGTTATTCCGGTGGTGGTGCTGGCGGCGGCGCGGGTGGCGGAGGCGGTGGTGGCTGGTAATTAAGCTGATGTTAATCGAAACAAGCTTTAACCATAAAGATAGTGTAGTA  >  NC_000913/1324531‑1324715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑