Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,380,158 C→T P63L (CCG→CTG)  ompG → outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,380,1580CT84.2% 596.9 / 77.7 221P63L (CCG→CTG) ompGouter membrane protein
Reads supporting (aligned to +/- strand):  ref base C (17/18);  new base T (96/90);  total (113/108)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 228 total aligned reads displayed.

CGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGA  >  NC_000913/1380068‑1380248
                                                                                          |                                                                                          
 gATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCtgtg                                                                                         <  1:1796059/93‑1 (MQ=255)
  aTGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTgg                                                                                        >  2:2378418/1‑93 (MQ=255)
   tGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGga                                                                                       <  2:5021345/93‑1 (MQ=255)
   tGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGga                                                                                       <  2:6303771/93‑1 (MQ=255)
      aCGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGaa                                                                                    >  1:6075346/1‑93 (MQ=255)
      aCGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGaa                                                                                    >  1:1109864/1‑93 (MQ=255)
       cGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGGGGAGAAt                                                                                   >  2:1792365/1‑93 (MQ=255)
       cGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAAt                                                                                   <  2:1314124/93‑1 (MQ=255)
        gAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGaagt                                                                                  >  2:260016/1‑91 (MQ=255)
         aaaTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTg                                                                                 >  2:764220/1‑93 (MQ=255)
         aaaTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTg                                                                                 >  2:6400970/1‑93 (MQ=255)
          aaTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGc                                                                                <  2:3586337/93‑1 (MQ=255)
          aaTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGc                                                                                <  2:5273231/93‑1 (MQ=255)
           aTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGGCTTCAGGCTATTTTAATGCCGCCAACGGGGTGTGGAGAATTGct                                                                               >  1:5821361/1‑93 (MQ=255)
           aTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGct                                                                               >  2:6639410/1‑93 (MQ=255)
           aTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGct                                                                               <  1:4787199/93‑1 (MQ=255)
           aTAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGct                                                                               >  1:3400150/1‑93 (MQ=255)
            tAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGctc                                                                              <  2:625114/93‑1 (MQ=255)
            aaGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAACTTTCAGTCTATTTTAATGCCGACAGCGGGCTGTGGAGAATTGctc                                                                              <  1:2282861/92‑1 (MQ=255)
              gAAAGCGTCGGGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTg                                                                            <  2:5135497/93‑1 (MQ=255)
              gAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTg                                                                            >  1:6164452/1‑93 (MQ=255)
              gAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTCAATGCCGCCAACGGGCCGTGGAGAATTGCTCTg                                                                            <  2:3732239/93‑1 (MQ=255)
                aaaCGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGc                                                                          >  2:2878373/1‑93 (MQ=255)
                 aaCGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGcc                                                                         >  2:6525170/1‑93 (MQ=255)
                 aaCGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGcc                                                                         >  1:6249602/1‑93 (MQ=255)
                 aaCGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGcc                                                                         <  2:6189821/93‑1 (MQ=255)
                     tCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCtatt                                                                     <  1:5531528/93‑1 (MQ=255)
                     tCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCtatt                                                                     >  1:3207741/1‑93 (MQ=255)
                      cGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCtatta                                                                    >  1:6300584/1‑93 (MQ=255)
                         gggTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCa                                                                 <  1:6258080/93‑1 (MQ=255)
                         gggTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCa                                                                 >  2:2207579/1‑93 (MQ=255)
                         gggTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCa                                                                 >  2:4326718/1‑93 (MQ=255)
                         gggTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCa                                                                 <  1:4851984/93‑1 (MQ=255)
                          ggTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAg                                                                >  1:948637/1‑93 (MQ=255)
                           gTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTTTGGAGAATTGCTCTGGCCTATTATCAga                                                               >  1:2949221/1‑92 (MQ=255)
                            ttATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGa                                                              <  2:4869914/93‑1 (MQ=255)
                            ttATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGa                                                              <  1:4720919/93‑1 (MQ=255)
                             tATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGaa                                                             <  1:5161361/93‑1 (MQ=255)
                             tATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGaa                                                             >  1:1056991/1‑93 (MQ=255)
                              aTGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAg                                                            >  2:5493128/1‑93 (MQ=255)
                               tGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAgg                                                           <  1:5639736/93‑1 (MQ=255)
                               tGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAgg                                                           <  1:3639063/93‑1 (MQ=255)
                                  cGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGcc                                                        <  2:3528767/93‑1 (MQ=255)
                                   gAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCg                                                       >  2:4613360/1‑93 (MQ=255)
                                     aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGt                                                     <  2:6075346/93‑1 (MQ=255)
                                     aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGt                                                     >  2:2486104/1‑93 (MQ=255)
                                     aGATATGGATGGGCTGGCGGAACCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGt                                                     >  1:4224550/1‑93 (MQ=255)
                                      gATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTa                                                    >  1:5683478/1‑93 (MQ=255)
                                      aatatGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTa                                                    <  2:5593953/92‑1 (MQ=255)
                                       atatGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAg                                                   <  2:3432528/93‑1 (MQ=255)
                                          tggatggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGAtt                                                <  2:545383/93‑1 (MQ=255)
                                           ggatggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATta                                               <  1:1224268/93‑1 (MQ=255)
                                            gatggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATtat                                              <  2:1295644/93‑1 (MQ=255)
                                            gatggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATtat                                              >  1:6639977/1‑93 (MQ=255)
                                              tggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAg                                            >  1:4261361/1‑93 (MQ=255)
                                              tggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAg                                            <  2:4899419/93‑1 (MQ=255)
                                                ggCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAgcg                                          >  1:3490411/1‑93 (MQ=255)
                                                  cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGcgcg                                        >  1:4559725/1‑93 (MQ=255)
                                                   tGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCgg                                       >  2:2268927/1‑93 (MQ=255)
                                                   tGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCgg                                       <  2:1913187/93‑1 (MQ=255)
                                                    ggCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCggg                                      >  1:2510411/1‑93 (MQ=255)
                                                    ggCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCggg                                      <  1:3799047/93‑1 (MQ=255)
                                                    ggCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCggg                                      <  1:5479682/93‑1 (MQ=255)
                                                     gCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGt                                     <  2:2582224/93‑1 (MQ=255)
                                                     gCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGt                                     >  2:2095495/1‑93 (MQ=255)
                                                     gCGGAGCCTTCAGTCTATTTTAATGCCACCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGt                                     <  2:1464800/93‑1 (MQ=255)
                                                      cGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAgg                                                               <  1:2263696/66‑1 (MQ=255)
                                                      cGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTa                                    <  2:4254535/93‑1 (MQ=255)
                                                       ggAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTaa                                   >  1:69774/1‑93 (MQ=255)
                                                       ggAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTaa                                   <  2:3938555/93‑1 (MQ=255)
                                                         aGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaac                                 <  2:5181865/93‑1 (MQ=255)
                                                           ccTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgt                               >  2:4211710/1‑93 (MQ=255)
                                                           ccTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCT‑GCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtg                              <  1:5251327/93‑1 (MQ=255)
                                                           ccTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgt                               <  1:1983136/93‑1 (MQ=255)
                                                            cTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtg                              <  2:3639020/93‑1 (MQ=255)
                                                            cTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtg                              >  1:759472/1‑93 (MQ=255)
                                                             ttCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGTCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtgg                             <  1:3923256/93‑1 (MQ=255)
                                                             ttCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtgg                             >  2:1434955/1‑93 (MQ=255)
                                                             ttCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtgg                             >  2:443139/1‑93 (MQ=255)
                                                                  tCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtggaacgt                        >  2:4725068/1‑93 (MQ=255)
                                                                  tCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtggaacgt                        >  2:2447162/1‑93 (MQ=255)
                                                                  tCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtggaacgt                        >  1:2733584/1‑93 (MQ=255)
                                                                    tATTTTAGTGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgtggaacgtgg                      >  1:6536582/1‑93 (MQ=255)
                                                                     aTTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGt                     >  2:948934/1‑93 (MQ=255)
                                                                       tttAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGttt                   <  1:6318550/93‑1 (MQ=255)
                                                                       tttAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGttt                   <  2:3204168/93‑1 (MQ=255)
                                                                         tAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGa                 <  2:5750519/93‑1 (MQ=255)
                                                                          aaTGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGGTTGAt                >  1:5717460/1‑93 (MQ=255)
                                                                          aaTGACGCCAACGGGCGGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGAt                <  2:3663834/93‑1 (MQ=255)
                                                                           aTGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATc               >  1:2305559/1‑93 (MQ=255)
                                                                             gccgccAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGTAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGc             >  2:4673720/1‑93 (MQ=255)
                                                                                  cAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGa        >  1:287464/1‑93 (MQ=255)
                                                                                    aCGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGc      >  1:6033431/1‑93 (MQ=255)
                                                                                    aCGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGc      >  1:1627936/1‑93 (MQ=255)
                                                                                    aCGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGc      <  2:5990651/93‑1 (MQ=255)
                                                                                       ggCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg   <  1:3721851/93‑1 (MQ=255)
                                                                                       ggCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg   >  1:4189856/1‑93 (MQ=255)
                                                                                       ggCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg   <  1:2084278/93‑1 (MQ=255)
                                                                                       ggCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg   <  2:1096793/93‑1 (MQ=255)
                                                                                       ggCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg   <  1:1453661/93‑1 (MQ=255)
                                                                                          |                                                                                          
CGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGA  >  NC_000913/1380068‑1380248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGT  >  NC_000913/1380065‑1380251
                                                                                             |                                                                                             
GCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCT                                                                                               <  SRR4302547.1576862/94‑1 (MQ=60)
    GATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGG                                                                                           <  SRR4302547.1831596/94‑1 (MQ=60)
     ATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGA                                                                                          >  SRR4302547.4637826/1‑94 (MQ=60)
        TACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAA                                                                                       >  SRR4302547.6201371/1‑94 (MQ=60)
        TACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAA                                                                                       <  SRR4302547.3575212/94‑1 (MQ=60)
        TACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAA                                                                                       >  SRR4302547.1132210/1‑94 (MQ=60)
          CGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATT                                                                                     <  SRR4302547.635462/94‑1 (MQ=60)
          CGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGCCTATTTTAATGCCGCCAACGGGCTGTGGAGcact                                                                                     >  SRR4302547.5566313/1‑90 (MQ=60)
            AAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGC                                                                                   >  SRR4302547.3185200/1‑94 (MQ=60)
            AAATAGAAAACGTCGAGGGTTATGG‑GAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCT                                                                                  >  SRR4302547.3758475/1‑94 (MQ=60)
             AATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGGCTTCAGGCTATTTTAATGCCGCCAACGGGGTGTGGAGAATTGCT                                                                                  >  SRR4302547.5941792/1‑94 (MQ=60)
             AATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCT                                                                                  >  SRR4302547.3469337/1‑94 (MQ=60)
              ATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTC                                                                                 <  SRR4302547.4886385/94‑1 (MQ=60)
              ATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTC                                                                                 <  SRR4302547.6266841/94‑1 (MQ=60)
               AAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAACTTTCAGTCTATTTTAATGCCGACAGCGGGCTGTGGAGAATTGCTCT                                                                                <  SRR4302547.2329070/94‑1 (MQ=60)
                AGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTG                                                                               <  SRR4302547.1896684/94‑1 (MQ=60)
                AGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTG                                                                               >  SRR4302547.6438997/1‑94 (MQ=60)
                AGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTG                                                                               <  SRR4302547.4336855/94‑1 (MQ=60)
                AGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTG                                                                               >  SRR4302547.6292397/1‑94 (MQ=60)
                   AAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCC                                                                            >  SRR4302547.6379430/1‑94 (MQ=60)
                   AAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCC                                                                            <  SRR4302547.4834246/94‑1 (MQ=60)
                    AACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCT                                                                           >  SRR4302547.4701446/1‑94 (MQ=60)
                    AACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCT                                                                           <  SRR4302547.5331731/94‑1 (MQ=60)
                      CGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTAT                                                                         >  SRR4302547.3804/1‑94 (MQ=60)
                       GTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATT                                                                        >  SRR4302547.3270378/1‑94 (MQ=60)
                       GTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATT                                                                        <  SRR4302547.678555/94‑1 (MQ=60)
                       GTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATT                                                                        >  SRR4302547.3271724/1‑94 (MQ=60)
                       GTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATT                                                                        <  SRR4302547.2029782/94‑1 (MQ=60)
                        TCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTA                                                                       <  SRR4302547.5645700/94‑1 (MQ=60)
                        TCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTA                                                                       >  SRR4302547.6431556/1‑94 (MQ=60)
                          GAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATC                                                                     >  SRR4302547.488138/1‑94 (MQ=60)
                          GAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATC                                                                     >  SRR4302547.4100894/1‑94 (MQ=60)
                           AGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTTCTGTGGCCTATTATCG                                                                    >  SRR4302547.5858257/1‑94 (MQ=60)
                           AGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCA                                                                    <  SRR4302547.1656534/94‑1 (MQ=60)
                           AGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCA                                                                    <  SRR4302547.1466767/94‑1 (MQ=60)
                            GGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAG                                                                   <  SRR4302547.4952524/94‑1 (MQ=60)
                            GGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAG                                                                   <  SRR4302547.6388079/94‑1 (MQ=60)
                            GGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAG                                                                   >  SRR4302547.967059/1‑94 (MQ=60)
                             GTTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGG                                                                  <  SRR4302547.5814478/94‑1 (MQ=60)
                             GGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTTTGGAGAATTGCTCTGGCCTATTATCAGA                                                                  >  SRR4302547.3007954/1‑94 (MQ=60)
                             GGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGG                                                                  <  SRR4302547.723388/94‑1 (MQ=60)
                              cacgacgctcttccgatcttgacattGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGA                                                                 <  SRR4302547.2309448/68‑1 (MQ=60)
                               TTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAA                                                                >  SRR4302547.5738591/1‑94 (MQ=60)
                               TTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAA                                                                <  SRR4302547.6384494/94‑1 (MQ=60)
                               TTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAA                                                                <  SRR4302547.5114505/94‑1 (MQ=60)
                               TTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAA                                                                <  SRR4302547.4818672/94‑1 (MQ=60)
                               TTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAA                                                                >  SRR4302547.1078015/1‑94 (MQ=60)
                                TATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAG                                                               <  SRR4302547.5267632/94‑1 (MQ=60)
                                 ATGTCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGG                                                              <  SRR4302547.1086268/94‑1 (MQ=60)
                                  TGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGG                                                             <  SRR4302547.5756150/94‑1 (MQ=60)
                                  TGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGG                                                             <  SRR4302547.3437770/94‑1 (MQ=60)
                                  TGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGG                                                             <  SRR4302547.3713283/94‑1 (MQ=60)
                                     CGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCG                                                          >  SRR4302547.2507538/1‑94 (MQ=60)
                                       AAGATATGGATGGGCTGGCGGAACCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGT                                                        >  SRR4302547.4310864/1‑94 (MQ=60)
                                        cttccgatcttgacattCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTA                                                       >  SRR4302547.1720734/18‑94 (MQ=60)
                                        AGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGGAGGGCCGGTA                                                       >  SRR4302547.3672633/1‑94 (MQ=60)
                                        AGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTA                                                       >  SRR4302547.5800828/1‑94 (MQ=60)
                                          ATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGA                                                     <  SRR4302547.1237272/94‑1 (MQ=60)
                                           TATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGAT                                                    <  SRR4302547.421499/94‑1 (MQ=60)
                                            ATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATT                                                   >  SRR4302547.1667469/1‑94 (MQ=60)
                                            ATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATT                                                   >  SRR4302547.2332867/1‑94 (MQ=60)
                                            ATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATT                                                   >  SRR4302547.5774923/1‑94 (MQ=60)
                                              GGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTAT                                                 >  SRR4302547.6778180/1‑94 (MQ=60)
                                              GGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTAT                                                 <  SRR4302547.1248959/94‑1 (MQ=60)
                                               GATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATA                                                <  SRR4302547.1609184/94‑1 (MQ=60)
                                               GATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATA                                                <  SRR4302547.913592/94‑1 (MQ=60)
                                                ATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAG                                               >  SRR4302547.6625673/1‑94 (MQ=60)
                                                ATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAG                                               >  SRR4302547.4348569/1‑94 (MQ=60)
                                                  GGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCG                                             >  SRR4302547.3561637/1‑94 (MQ=60)
                                                    GCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCG                                           <  SRR4302547.2471772/94‑1 (MQ=60)
                                                    GCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCG                                           <  SRR4302547.2289836/94‑1 (MQ=60)
                                                    GCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCG                                           >  SRR4302547.4654058/1‑94 (MQ=60)
                                                      TGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGG                                         >  SRR4302547.2561154/1‑94 (MQ=60)
                                                      TGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGG                                         >  SRR4302547.5940859/1‑94 (MQ=60)
                                                       GGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGT                                        <  SRR4302547.3876495/94‑1 (MQ=60)
                                                       GGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGT                                        <  SRR4302547.5592833/94‑1 (MQ=60)
                                                         CGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAA                                      >  SRR4302547.71077/1‑94 (MQ=60)
                                                         CGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAA                                      <  SRR4302547.6689721/94‑1 (MQ=60)
                                                           GAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAAC                                    <  SRR4302547.6188564/94‑1 (MQ=60)
                                                            AGCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACG                                   >  SRR4302547.2317280/1‑94 (MQ=60)
                                                             GCCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGT                                  >  SRR4302547.3946137/1‑94 (MQ=60)
                                                              CCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTG                                 >  SRR4302547.773790/1‑94 (MQ=60)
                                                              CCTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCT‑GCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGG                                <  SRR4302547.5359456/94‑1 (MQ=60)
                                                              CCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTG                                 <  SRR4302547.2022521/94‑1 (MQ=60)
                                                               CTTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGG                                >  SRR4302547.3004796/1‑94 (MQ=60)
                                                                TTCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGTCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGA                               <  SRR4302547.4003028/94‑1 (MQ=60)
                                                                 TCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAA                              <  SRR4302547.2705258/94‑1 (MQ=60)
                                                                 TCAGTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAA                              <  SRR4302547.6437195/94‑1 (MQ=60)
                                                                    GTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGT                           >  SRR4302547.2788446/1‑94 (MQ=60)
                                                                    GTCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGT                           >  SRR4302547.5078857/1‑94 (MQ=60)
                                                                     TCTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTG                          >  SRR4302547.5888097/1‑94 (MQ=60)
                                                                      CTATTTTAGTGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGG                         >  SRR4302547.6672605/1‑94 (MQ=60)
                                                                      CTATTTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGG                         >  SRR4302547.5584597/1‑94 (MQ=60)
                                                                          TTTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTG                     <  SRR4302547.6449934/94‑1 (MQ=60)
                                                                           TTAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGA                    <  SRR4302547.763162/94‑1 (MQ=60)
                                                                            TAATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGGTTGAT                   >  SRR4302547.5835556/1‑94 (MQ=60)
                                                                             AATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATC                  >  SRR4302547.2352290/1‑94 (MQ=60)
                                                                             AATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATC                  <  SRR4302547.4628394/94‑1 (MQ=60)
                                                                             AATGCCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATC                  <  SRR4302547.426934/94‑1 (MQ=60)
                                                                                 CCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCC              >  SRR4302547.5944960/1‑94 (MQ=60)
                                                                                 CCGCCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCC              <  SRR4302547.6309093/94‑1 (MQ=60)
                                                                                    CCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGA           <  SRR4302547.3486006/94‑1 (MQ=60)
                                                                                    CCAACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGA           >  SRR4302547.292804/1‑94 (MQ=60)
                                                                                      AACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGC         >  SRR4302547.2276461/1‑94 (MQ=60)
                                                                                      AACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGC         >  SRR4302547.1660139/1‑94 (MQ=60)
                                                                                      AACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGC         >  SRR4302547.5597512/1‑94 (MQ=60)
                                                                                      AACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGC         >  SRR4302547.6158637/1‑94 (MQ=60)
                                                                                       ACGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCT        <  SRR4302547.292472/94‑1 (MQ=60)
                                                                                        CGGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTG       >  SRR4302547.5729964/1‑94 (MQ=60)
                                                                                         GGGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGG      >  SRR4302547.4275299/1‑94 (MQ=60)
                                                                                          GGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGA     <  SRR4302547.3797827/94‑1 (MQ=60)
                                                                                          GGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGA     <  SRR4302547.1482721/94‑1 (MQ=60)
                                                                                          GGCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGA     <  SRR4302547.2125836/94‑1 (MQ=60)
                                                                                           GCTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAG    >  SRR4302547.3732969/1‑94 (MQ=60)
                                                                                           GCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAG    <  SRR4302547.6419815/94‑1 (MQ=60)
                                                                                            CTGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGG   <  SRR4302547.4579490/94‑1 (MQ=60)
                                                                                             TGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGG  >  SRR4302547.5885283/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGT  >  NC_000913/1380065‑1380251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑