Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,529,193 T→C V195A (GTA→GCA)  b1458 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,529,1930TC88.0% 589.1 / 62.6 209V195A (GTA→GCA) b1458hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/15);  new base C (98/86);  total (108/101)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 214 total aligned reads displayed.

AAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTA  >  NC_000913/1529101‑1529282
                                                                                            |                                                                                         
aaaGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGt                                                                                           >  1:653954/1‑93 (MQ=255)
aaaGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGt                                                                                           >  1:4968194/1‑93 (MQ=255)
     aaaaTTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAg                                                                                      >  1:3703186/1‑93 (MQ=255)
       aaTTTTATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGc                                                                                    <  2:3640257/93‑1 (MQ=255)
       aaTTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGc                                                                                    <  1:3178255/93‑1 (MQ=255)
        aTTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCa                                                                                   <  1:1115944/93‑1 (MQ=255)
        aTTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCa                                                                                   >  1:4539098/1‑93 (MQ=255)
         tttGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCaa                                                                                  <  2:1320789/93‑1 (MQ=255)
            gATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCa                                                                               <  1:938061/93‑1 (MQ=255)
               aaCCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAggg                                                                            <  2:1963953/93‑1 (MQ=255)
                aCCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAgggg                                                                           >  1:4416276/1‑93 (MQ=255)
                 ccATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGa                                                                          >  1:1033776/1‑93 (MQ=255)
                 ccATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGa                                                                          <  2:1604701/93‑1 (MQ=255)
                  cATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGaa                                                                         >  1:5396991/1‑93 (MQ=255)
                   aTTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGaaa                                                                        >  1:3637100/1‑93 (MQ=255)
                   aTTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGaaa                                                                        <  2:5753494/93‑1 (MQ=255)
                    ttGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAAt                                                                       <  1:5164240/93‑1 (MQ=255)
                    ttGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAAt                                                                       <  1:1211862/93‑1 (MQ=255)
                     tGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAAtt                                                                      <  2:4491524/93‑1 (MQ=255)
                        tGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTAca                                                                   <  2:5852433/93‑1 (MQ=255)
                        tGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTAca                                                                   >  2:4242812/1‑93 (MQ=255)
                           tATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTAcatca                                                                >  1:3782361/1‑93 (MQ=255)
                           tATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTAcatca                                                                >  1:2997395/1‑93 (MQ=255)
                           tATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTAcatca                                                                >  2:1491768/1‑93 (MQ=255)
                            aTGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTAcatcat                                                               >  2:231370/1‑93 (MQ=255)
                            aTGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTAcatcat                                                               >  2:3236437/1‑93 (MQ=255)
                            aTGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCGGGCAGCAAAAGGCAATCAGGGGAAATTAcatcat                                                               <  2:29254/93‑1 (MQ=255)
                              gggCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGc                                                             >  1:73196/1‑93 (MQ=255)
                               ggCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCa                                                            <  2:4247450/93‑1 (MQ=255)
                                gCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCAt                                                           <  2:4454077/93‑1 (MQ=255)
                                  tGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTc                                                         <  1:197909/93‑1 (MQ=255)
                                     cAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAg                                                      <  2:1816056/93‑1 (MQ=255)
                                      aGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAgg                                                     >  2:2089806/1‑93 (MQ=255)
                                       gAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGa                                                    >  2:5125320/1‑93 (MQ=255)
                                          aGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGaaaa                                                 <  1:5564302/93‑1 (MQ=255)
                                          aGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGaaaa                                                 >  1:4389178/1‑93 (MQ=255)
                                           gATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGaaaat                                                >  1:1567632/1‑92 (MQ=255)
                                            atatCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAAt                                               >  2:4618015/1‑93 (MQ=255)
                                               tCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTc                                            >  1:1176749/1‑93 (MQ=255)
                                                cGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTcc                                           <  2:2669071/93‑1 (MQ=255)
                                                 gCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCt                                          >  2:3178454/1‑93 (MQ=255)
                                                 gCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCt                                          <  2:5939201/93‑1 (MQ=255)
                                                 gCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCt                                          <  2:3756540/93‑1 (MQ=255)
                                                 gCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCt                                          >  2:556931/1‑93 (MQ=255)
                                                  cTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTg                                         >  2:6046506/1‑93 (MQ=255)
                                                   ttCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGt                                        <  2:2071059/93‑1 (MQ=255)
                                                   ttCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGt                                        >  2:4096133/1‑93 (MQ=255)
                                                    tCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTa                                       >  1:879183/1‑93 (MQ=255)
                                                     cGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTaa                                      <  2:6259797/93‑1 (MQ=255)
                                                      gAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTaaa                                     >  2:3453607/1‑93 (MQ=255)
                                                      gAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTaaa                                     <  2:5077600/93‑1 (MQ=255)
                                                       aaGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAAt                                    <  1:1867312/93‑1 (MQ=255)
                                                       aaGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAAt                                    <  2:3725445/93‑1 (MQ=255)
                                                        aGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAAtg                                   <  1:2224593/93‑1 (MQ=255)
                                                          aTCATAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAAtgtg                                 <  1:2723977/93‑1 (MQ=255)
                                                           tCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATgtgt                                >  1:4690544/1‑93 (MQ=255)
                                                           tCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATgtgt                                >  1:5972899/1‑93 (MQ=255)
                                                             aaaGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGttt                              >  2:1409274/1‑93 (MQ=255)
                                                             aaaGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGttt                              >  2:2987793/1‑93 (MQ=255)
                                                             aaaGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGttt                              <  1:2090858/93‑1 (MQ=255)
                                                             aaaGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGttt                              >  2:3994687/1‑93 (MQ=255)
                                                             aaaGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGttt                              >  1:4870998/1‑93 (MQ=255)
                                                              aaGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGtttt                             >  2:5032864/1‑93 (MQ=255)
                                                               aGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTc                            >  2:507353/1‑93 (MQ=255)
                                                               aGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTc                            <  2:548828/93‑1 (MQ=255)
                                                               aGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTc                            >  1:1420042/1‑93 (MQ=255)
                                                                gATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCt                           <  1:2920074/93‑1 (MQ=255)
                                                                gATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCt                           >  2:6137138/1‑93 (MQ=255)
                                                                gATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCt                           <  2:3928362/93‑1 (MQ=255)
                                                                  tAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTaa                         >  1:6620253/1‑93 (MQ=255)
                                                                  tAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTaa                         >  1:1643321/1‑93 (MQ=255)
                                                                    aaaaaGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAAtt                       <  2:3313171/93‑1 (MQ=255)
                                                                     aaaaGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATta                      >  1:1152667/1‑93 (MQ=255)
                                                                      aaaGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATtat                     >  1:3472788/1‑93 (MQ=255)
                                                                         gCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATaaa                  >  1:4855724/1‑93 (MQ=255)
                                                                         gCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATaaa                  <  2:5423382/93‑1 (MQ=255)
                                                                         gCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATaaa                  <  2:2856324/93‑1 (MQ=255)
                                                                         gCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATaaa                  >  1:1753747/1‑93 (MQ=255)
                                                                          cAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAg                 <  1:2072228/93‑1 (MQ=255)
                                                                           aGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAgg                <  2:1481480/93‑1 (MQ=255)
                                                                           aGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAgg                <  1:498701/93‑1 (MQ=255)
                                                                            gATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAACGc               >  2:2360376/1‑93 (MQ=255)
                                                                            gATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGc               >  1:264201/1‑93 (MQ=255)
                                                                             aTTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCg              >  1:2321600/1‑93 (MQ=255)
                                                                             aTTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCg              <  1:5502583/93‑1 (MQ=255)
                                                                              ttATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGa             <  1:2798394/93‑1 (MQ=255)
                                                                                aTCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGAtt           <  1:2567726/93‑1 (MQ=255)
                                                                                aTCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGAtt           <  1:4952002/93‑1 (MQ=255)
                                                                                 tcttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTc          >  2:4191673/1‑93 (MQ=255)
                                                                                 tcttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTc          >  1:4647155/1‑93 (MQ=255)
                                                                                  cttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCg         >  2:3351648/1‑93 (MQ=255)
                                                                                  cttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCg         >  2:3580996/1‑93 (MQ=255)
                                                                                   ttctGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGt        <  1:5485386/93‑1 (MQ=255)
                                                                                   ttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGt        >  2:4902696/1‑93 (MQ=255)
                                                                                   ttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGt        >  1:5676007/1‑93 (MQ=255)
                                                                                   ttctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGt        >  2:1206127/1‑93 (MQ=255)
                                                                                    tctGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTCTTCTAATTATAAAGGCGATTCGtt       <  1:6405711/93‑1 (MQ=255)
                                                                                       ggCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAg    <  2:2762343/93‑1 (MQ=255)
                                                                                        gcagcaAAAGGCAATCAGGGGAAAATACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGt   <  1:5320711/93‑1 (MQ=255)
                                                                                         cAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTa  <  2:249609/93‑1 (MQ=255)
                                                                                            |                                                                                         
AAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTA  >  NC_000913/1529101‑1529282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCA  >  NC_000913/1529100‑1529286
                                                                                             |                                                                                             
TAAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGT                                                                                               >  SRR4302547.666000/1‑94 (MQ=60)
TAAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGT                                                                                               >  SRR4302547.5071005/1‑94 (MQ=60)
TAAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGC                                                                                               >  SRR4302547.1011742/1‑94 (MQ=60)
 AAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCA                                                                                              <  SRR4302547.4509577/94‑1 (MQ=60)
  AAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAA                                                                                             <  SRR4302547.2122068/94‑1 (MQ=60)
   AGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAA                                                                                            >  SRR4302547.4671753/1‑94 (MQ=60)
     AAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAG                                                                                          <  SRR4302547.6776581/94‑1 (MQ=60)
     AAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAG                                                                                          >  SRR4302547.3778775/1‑94 (MQ=60)
      AAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGG                                                                                         >  SRR4302547.2130663/1‑94 (MQ=60)
       AAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGC                                                                                        >  SRR4302547.3861036/1‑94 (MQ=60)
        AATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGACGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCA                                                                                       <  SRR4302547.3397648/94‑1 (MQ=60)
        AATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCA                                                                                       <  SRR4302547.3241570/94‑1 (MQ=60)
        AATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCA                                                                                       <  SRR4302547.2406930/94‑1 (MQ=60)
        AATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCA                                                                                       >  SRR4302547.4632916/1‑94 (MQ=60)
         ATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAA                                                                                      <  SRR4302547.586406/94‑1 (MQ=60)
         ATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAA                                                                                      <  SRR4302547.1138414/94‑1 (MQ=60)
           TTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATC                                                                                    >  SRR4302547.298356/1‑94 (MQ=60)
           TTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATC                                                                                    <  SRR4302547.2943169/94‑1 (MQ=60)
             GATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAG                                                                                  <  SRR4302547.5062640/94‑1 (MQ=60)
             GATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAG                                                                                  <  SRR4302547.315043/94‑1 (MQ=60)
             GATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAG                                                                                  <  SRR4302547.956225/94‑1 (MQ=60)
              ATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGG                                                                                 <  SRR4302547.3821429/94‑1 (MQ=60)
                AACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGG                                                                               >  SRR4302547.6702653/1‑94 (MQ=60)
                AACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGG                                                                               >  SRR4302547.4507391/1‑94 (MQ=60)
                AACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGG                                                                               <  SRR4302547.5567850/94‑1 (MQ=60)
                 ACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGA                                                                              >  SRR4302547.1054112/1‑94 (MQ=60)
                  CCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAA                                                                             >  SRR4302547.5084286/1‑94 (MQ=60)
                  CCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAA                                                                             >  SRR4302547.5508501/1‑94 (MQ=60)
                   CATTGATGCTATGGGCTGTCTGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAA                                                                            >  SRR4302547.5110386/1‑94 (MQ=60)
                   CATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAA                                                                            <  SRR4302547.1735611/94‑1 (MQ=60)
                   CATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAA                                                                            >  SRR4302547.5783796/1‑94 (MQ=60)
                   CATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAA                                                                            >  SRR4302547.3711273/1‑94 (MQ=60)
                     TTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATT                                                                          <  SRR4302547.5270568/94‑1 (MQ=60)
                     TTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATT                                                                          <  SRR4302547.1236324/94‑1 (MQ=60)
                         TGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACAT                                                                      >  SRR4302547.5615789/1‑94 (MQ=60)
                           CTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCA                                                                    >  SRR4302547.3859490/1‑94 (MQ=60)
                           CTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCA                                                                    >  SRR4302547.3057018/1‑94 (MQ=60)
                            TATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCAT                                                                   >  SRR4302547.5297279/1‑94 (MQ=60)
                              TGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGC                                                                 <  SRR4302547.265464/94‑1 (MQ=60)
                              TGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGC                                                                 >  SRR4302547.74568/1‑94 (MQ=60)
                              TGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGC                                                                 <  SRR4302547.931000/94‑1 (MQ=60)
                                GGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCAT                                                               >  SRR4302547.1662884/1‑94 (MQ=60)
                                   TGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCG                                                            <  SRR4302547.201607/94‑1 (MQ=60)
                                   TGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCG                                                            <  SRR4302547.5171318/94‑1 (MQ=60)
                                   TGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCG                                                            >  SRR4302547.2848022/1‑94 (MQ=60)
                                    GTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGA                                                           >  SRR4302547.108731/1‑94 (MQ=60)
                                        GAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAA                                                       <  SRR4302547.126524/94‑1 (MQ=60)
                                          AAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAA                                                     <  SRR4302547.6181417/94‑1 (MQ=60)
                                          AAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAA                                                     >  SRR4302547.4479489/1‑94 (MQ=60)
                                          AAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAA                                                     <  SRR4302547.1756100/94‑1 (MQ=60)
                                           AGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAA                                                    <  SRR4302547.5679060/94‑1 (MQ=60)
                                           AGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAT                                                    >  SRR4302547.1598847/1‑94 (MQ=60)
                                             ATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATT                                                  <  SRR4302547.1562335/94‑1 (MQ=60)
                                               ATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGGAATTACATCATGCATTCGAGGAAAAATTTC                                                >  SRR4302547.2231885/1‑94 (MQ=60)
                                               ATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTC                                                >  SRR4302547.1200521/1‑94 (MQ=60)
                                                TCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCC                                               >  SRR4302547.5670708/1‑94 (MQ=60)
                                                 CGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCT                                              <  SRR4302547.4736921/94‑1 (MQ=60)
                                                    TTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTA                                           >  SRR4302547.896075/1‑94 (MQ=60)
                                                        AAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATG                                       <  SRR4302547.1904186/94‑1 (MQ=60)
                                                        AAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATG                                       >  SRR4302547.6618290/1‑94 (MQ=60)
                                                        AAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATG                                       <  SRR4302547.3716009/94‑1 (MQ=60)
                                                         AGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGT                                      <  SRR4302547.2269419/94‑1 (MQ=60)
                                                         AGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGT                                      <  SRR4302547.494592/94‑1 (MQ=60)
                                                          GATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTG                                     >  SRR4302547.2711783/1‑94 (MQ=60)
                                                           ATCATAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGT                                    <  SRR4302547.2778673/94‑1 (MQ=60)
                                                           ATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGT                                    >  SRR4302547.6096783/1‑94 (MQ=60)
                                                           ATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGT                                    >  SRR4302547.4787692/1‑94 (MQ=60)
                                                            TCAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTT                                   >  SRR4302547.5846838/1‑94 (MQ=60)
                                                             CAAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTT                                  >  SRR4302547.4971939/1‑94 (MQ=60)
                                                              AAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTT                                 >  SRR4302547.3381511/1‑94 (MQ=60)
                                                              AAAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTT                                 <  SRR4302547.2132536/94‑1 (MQ=60)
                                                               AAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTC                                >  SRR4302547.1448473/1‑94 (MQ=60)
                                                               AAGATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTC                                >  SRR4302547.4632313/1‑94 (MQ=60)
                                                                 GATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTA                              <  SRR4302547.2978300/94‑1 (MQ=60)
                                                                  ATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAA                             >  SRR4302547.6758047/1‑94 (MQ=60)
                                                                  ATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAA                             >  SRR4302547.2068391/1‑94 (MQ=60)
                                                                  ATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAA                             >  SRR4302547.1202996/1‑94 (MQ=60)
                                                                  ATAAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAA                             >  SRR4302547.1675786/1‑94 (MQ=60)
                                                                   TAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAAT                            <  SRR4302547.2342816/94‑1 (MQ=60)
                                                                    AAAAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATT                           >  SRR4302547.1993415/1‑94 (MQ=60)
                                                                     AAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTA                          >  SRR4302547.1175997/1‑94 (MQ=60)
                                                                     AAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTA                          >  SRR4302547.1175998/1‑94 (MQ=60)
                                                                      AAAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTAT                         >  SRR4302547.3543608/1‑94 (MQ=60)
                                                                       AAAGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATA                        >  SRR4302547.4779650/1‑94 (MQ=60)
                                                                         AGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAA                      >  SRR4302547.4956327/1‑94 (MQ=60)
                                                                         AGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAA                      >  SRR4302547.2534763/1‑94 (MQ=60)
                                                                         AGCAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAA                      >  SRR4302547.1788425/1‑94 (MQ=60)
                                                                           CAGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGG                    <  SRR4302547.2113581/94‑1 (MQ=60)
                                                                            AGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGC                   <  SRR4302547.507795/94‑1 (MQ=60)
                                                                            AGATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGC                   >  SRR4302547.269137/1‑94 (MQ=60)
                                                                             GATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCG                  >  SRR4302547.2368658/1‑94 (MQ=60)
                                                                             GATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCG                  <  SRR4302547.5037530/94‑1 (MQ=60)
                                                                              ATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGA                 <  SRR4302547.5616196/94‑1 (MQ=60)
                                                                              ATTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGA                 <  SRR4302547.4496488/94‑1 (MQ=60)
                                                                               TTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGAT                <  SRR4302547.1886507/94‑1 (MQ=60)
                                                                               TTATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGAT                <  SRR4302547.2854418/94‑1 (MQ=60)
                                                                                 ATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTC              >  SRR4302547.4743418/1‑94 (MQ=60)
                                                                                 ATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTC              <  SRR4302547.5054523/94‑1 (MQ=60)
                                                                                 ATCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTC              <  SRR4302547.2619586/94‑1 (MQ=60)
                                                                                  TCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCG             <  SRR4302547.3666776/94‑1 (MQ=60)
                                                                                  TCTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATGCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCG             <  SRR4302547.5490792/94‑1 (MQ=60)
                                                                                   CTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGT            >  SRR4302547.892893/1‑94 (MQ=60)
                                                                                   CTTCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGT            >  SRR4302547.5793206/1‑94 (MQ=60)
                                                                                    TTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTT           <  SRR4302547.5598660/94‑1 (MQ=60)
                                                                                     TCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTT          <  SRR4302547.4789027/94‑1 (MQ=60)
                                                                                     TCTGGCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTCTTCTAATTATAAAGGCGATTCGTTT          <  SRR4302547.6539306/94‑1 (MQ=60)
                                                                                         GCAGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTA      <  SRR4302547.1195710/94‑1 (MQ=60)
                                                                                         GCAGCAAAAGGCAATCAGGGGAAAATACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTA      <  SRR4302547.5430407/94‑1 (MQ=60)
                                                                                           AGCAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACG    >  SRR4302547.3473820/1‑94 (MQ=60)
                                                                                             CAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCA  >  SRR4302547.6709143/1‑94 (MQ=60)
                                                                                             |                                                                                             
TAAAGAAAAATTTGATAACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCA  >  NC_000913/1529100‑1529286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑