Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,357	C→T	P303P (CCG→CCA)	wza ←	putative polysaccharide export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,357	0	C	T	87.4%	733.2 / 71.5	246	P303P (CCG→CCA)	wza	putative polysaccharide export protein
Reads supporting (aligned to +/- strand): ref base C (20/11); new base T (109/106); total (129/117)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01

Only 100 of 253 total aligned reads displayed.

TTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGC  >  NC_000913/2134267‑2134445

ttCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTgg                                                                                        >  2:2370944/1‑93 (MQ=255)

  cTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGAc                                                                                      <  2:6448659/93‑1 (MQ=255)

   tGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACg                                                                                     >  1:2785266/1‑93 (MQ=255)

    gTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGa                                                                                    >  2:873887/1‑93 (MQ=255)

     tCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGaa                                                                                   <  2:4197229/93‑1 (MQ=255)

     tCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGaa                                                                                   >  2:625639/1‑93 (MQ=255)

      cGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAAt                                                                                  >  1:4656056/1‑93 (MQ=255)

         cATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGAc                                                                               >  1:5757716/1‑93 (MQ=255)

            cACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACaaa                                                                            <  1:1814554/93‑1 (MQ=255)

             aCCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAg                                                                           >  2:4612433/1‑93 (MQ=255)

              ccAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGa                                                                          <  2:2106197/93‑1 (MQ=255)

               cAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGAt                                                                         <  1:2614649/93‑1 (MQ=255)

                aGCGACGTGGCATCGGACGTATCCAGCTGGTAGATATTGGGAATCTTGCCGTGACGCCCGACCTCGTCTTTCTGTGTGCGAATGACCAAGATg                                                                        >  1:4872377/1‑93 (MQ=255)

                 gCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGc                                                                       >  1:6506221/1‑93 (MQ=255)

                 gCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGc                                                                       <  2:5432948/93‑1 (MQ=255)

                    aCGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGc                                                                    >  1:5973391/1‑93 (MQ=255)

                     cGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCt                                                                   <  2:1979279/93‑1 (MQ=255)

                        ggCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGc                                                                <  1:4235677/93‑1 (MQ=255)

                         gCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCg                                                               <  1:3225304/93‑1 (MQ=255)

                          cATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGt                                                              <  2:3274321/93‑1 (MQ=255)

                            tCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTGGCCTTTCACCGACCGATTACCAAAGAGGCC‑CCGGCGtttg                                                           >  2:2063124/1‑91 (MQ=255)

                            tCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTg                                                            >  2:4630139/1‑93 (MQ=255)

                            tCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTg                                                            >  1:3967628/1‑93 (MQ=255)

                            tCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTg                                                            >  1:1232327/1‑93 (MQ=255)

                              ggACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGa                                                          <  1:4823754/93‑1 (MQ=255)

                               gACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAg                                                         >  2:3701610/1‑93 (MQ=255)

                                  atatCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAggtg                                                      >  1:3518206/1‑93 (MQ=255)

                                   tatCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAggtgg                                                     >  1:1387623/1‑93 (MQ=255)

                                     tCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTc                                                   >  1:2758055/1‑93 (MQ=255)

                                      ccAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCa                                                  >  1:1518242/1‑93 (MQ=255)

                                      ccAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCa                                                  >  1:3736916/1‑93 (MQ=255)

                                      ccAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCa                                                  >  2:2920613/1‑93 (MQ=255)

                                       cAGCTGGTNGNTATTGGCAATCTTNCNGTNACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGACAt                                                 <  1:1150920/93‑1 (MQ=255)

                                       cAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCAt                                                 >  1:2319248/1‑93 (MQ=255)

                                        aGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATg                                                >  1:5204154/1‑93 (MQ=255)

                                        aGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATg                                                >  2:6605734/1‑93 (MQ=255)

                                         gCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGt                                               >  1:4355755/1‑93 (MQ=255)

                                         gCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGt                                               >  1:3804261/1‑93 (MQ=255)

                                           tGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCg                                             >  2:6526064/1‑93 (MQ=255)

                                           tGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCg                                             >  1:2682252/1‑93 (MQ=255)

                                            ggTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGa                                            >  1:6580563/1‑93 (MQ=255)

                                            ggTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGa                                            >  2:384242/1‑93 (MQ=255)

                                             gTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGAt                                           >  1:2098617/1‑93 (MQ=255)

                                             gTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGAt                                           <  1:3175464/93‑1 (MQ=255)

                                             gTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGAt                                           >  2:1386757/1‑93 (MQ=255)

                                             gTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCCTTCAGTTGACGAATGACAAAGATGCAGCTGGCGATGGAGGTTGTCATTGCGAt                                           >  1:3591075/1‑93 (MQ=255)

                                              tAGATATTGGCTATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATg                                          >  1:5011963/1‑93 (MQ=255)

                                                gATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGcc                                        <  2:2225495/93‑1 (MQ=255)

                                                gATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGcc                                        <  1:4327158/93‑1 (MQ=255)

                                                gATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGcc                                        >  1:4697795/1‑93 (MQ=255)

                                                  tatTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCtt                                      >  2:2658774/1‑93 (MQ=255)

                                                  tatTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCtt                                      <  1:1203366/93‑1 (MQ=255)

                                                   atTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTc                                     <  1:6164397/93‑1 (MQ=255)

                                                     tGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAg                                   >  1:2651847/1‑93 (MQ=255)

                                                      ggCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGc                                  >  1:4441907/1‑93 (MQ=255)

                                                       gCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCa                                 <  1:3443076/93‑1 (MQ=255)

                                                        cAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCAt                                >  1:1624941/1‑93 (MQ=255)

                                                         aaTCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCAtt                               >  2:5755115/1‑93 (MQ=255)

                                                          aTCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTg                              >  2:5888328/1‑93 (MQ=255)

                                                          aTCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTg                              >  1:937000/1‑93 (MQ=255)

                                                          aTCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTg                              >  2:1945075/1‑93 (MQ=255)

                                                           tCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGc                             <  1:368675/93‑1 (MQ=255)

                                                            cTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGcc                            <  2:4536550/93‑1 (MQ=255)

                                                             ttGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGccc                           <  1:538175/93‑1 (MQ=255)

                                                             ttGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGccc                           >  2:1790070/1‑93 (MQ=255)

                                                             ttGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGccc                           <  1:4064092/93‑1 (MQ=255)

                                                             ttGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGccc                           >  2:251615/1‑93 (MQ=255)

                                                             ttGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGccc                           <  1:5331278/93‑1 (MQ=255)

                                                             ttGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGccc                           <  2:682834/93‑1 (MQ=255)

                                                               gCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAg                         <  1:4319099/93‑1 (MQ=255)

                                                               gCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAg                         <  2:3347239/93‑1 (MQ=255)

                                                               gCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAg                         <  2:1707304/93‑1 (MQ=255)

                                                                ccGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAgg                        >  2:178488/1‑93 (MQ=255)

                                                                  gTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCTTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGc                      >  2:4327760/1‑93 (MQ=255)

                                                                  gTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGc                      >  1:802247/1‑93 (MQ=255)

                                                                   ttACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCt                     >  1:1755091/1‑93 (MQ=255)

                                                                   ttACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCt                     <  2:6244506/93‑1 (MQ=255)

                                                                   ttACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCt                     >  2:4731134/1‑93 (MQ=255)

                                                                   ttACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCt                     >  1:6244916/1‑93 (MQ=255)

                                                                    tACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCtt                    >  1:742993/1‑93 (MQ=255)

                                                                    tACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCtt                    <  2:5997942/93‑1 (MQ=255)

                                                                     aCGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTtc                   >  2:414880/1‑93 (MQ=255)

                                                                         ccgcccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTtcagtc               >  2:3047374/1‑93 (MQ=255)

                                                                         ccgcccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTtcagtc               <  2:5693596/93‑1 (MQ=255)

                                                                           gcccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTtcagtcag             >  2:3486267/1‑93 (MQ=255)

                                                                            cccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTGGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAgg            >  2:3449508/1‑93 (MQ=255)

                                                                            cccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAgg            >  2:4858085/1‑93 (MQ=255)

                                                                             ccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAggg           <  2:1254732/93‑1 (MQ=255)

                                                                             ccTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAggg           >  2:3102395/1‑93 (MQ=255)

                                                                              cTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGt          <  2:1638170/93‑1 (MQ=255)

                                                                              cTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGt          >  1:254572/1‑93 (MQ=255)

                                                                                cGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCa        >  1:2398758/1‑93 (MQ=255)

                                                                                cGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCa        >  2:3733733/1‑93 (MQ=255)

                                                                                 gCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCAt       <  1:1891110/93‑1 (MQ=255)

                                                                                  ccTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATg      >  2:5095841/1‑93 (MQ=255)

                                                                                  ccTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATg      >  1:4523487/1‑93 (MQ=255)

                                                                                   cTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGc     <  1:1715685/93‑1 (MQ=255)

                                                                                    tttCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGcc    >  1:3336947/1‑93 (MQ=255)

                                                                                      tCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGc  <  2:4217829/93‑1 (MQ=255)

                                                                                      tCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGc  <  2:5342715/93‑1 (MQ=255)

TTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGC  >  NC_000913/2134267‑2134445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAATTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGCTAAAG  >  NC_000913/2134264‑2134450

GAATTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGT                                                                                               <  SRR4302547.2981330/94‑1 (MQ=60)

 AATTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTG                                                                                              <  SRR4302547.4484411/94‑1 (MQ=60)

 AATTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTG                                                                                              <  SRR4302547.1786493/94‑1 (MQ=60)

  ATTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGG                                                                                             <  SRR4302547.6366376/94‑1 (MQ=60)

    TCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGAC                                                                                           <  SRR4302547.4192680/94‑1 (MQ=60)

     CTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACG                                                                                          >  SRR4302547.3560002/1‑94 (MQ=60)

     CTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACG                                                                                          >  SRR4302547.2841054/1‑94 (MQ=60)

     CTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACG                                                                                          <  SRR4302547.4847399/94‑1 (MQ=60)

     CTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACG                                                                                          <  SRR4302547.6345007/94‑1 (MQ=60)

        TCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAAT                                                                                       >  SRR4302547.4752483/1‑94 (MQ=60)

         CGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATG                                                                                      <  SRR4302547.1253552/94‑1 (MQ=60)

          GCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGA                                                                                     >  SRR4302547.2861741/1‑94 (MQ=60)

           CCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGAC                                                                                    >  SRR4302547.5876773/1‑94 (MQ=60)

            CATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACA                                                                                   <  SRR4302547.1240733/94‑1 (MQ=60)

            CATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACA                                                                                   <  SRR4302547.4089711/94‑1 (MQ=60)

              TCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAA                                                                                 <  SRR4302547.2640123/94‑1 (MQ=60)

               CACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAG                                                                                <  SRR4302547.1850401/94‑1 (MQ=60)

                CCCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGA                                                                               <  SRR4302547.4029364/94‑1 (MQ=60)

                 CCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGAT                                                                              <  SRR4302547.6273837/94‑1 (MQ=60)

                 CCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGAT                                                                              <  SRR4302547.843074/94‑1 (MQ=60)

                  CAGCGACGTGGCATCGGACGTATCCAGCTGGTAGATATTGGGAATCTTGCCGTGACGCCCGACCTCGTCTTTCTGTGTGCGAATGACCAAGATG                                                                             >  SRR4302547.4973344/1‑94 (MQ=50)

                  CAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATG                                                                             <  SRR4302547.4962519/94‑1 (MQ=60)

                  CAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATG                                                                             <  SRR4302547.2667341/94‑1 (MQ=60)

                  CAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATG                                                                             <  SRR4302547.2712770/94‑1 (MQ=60)

                   AGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGC                                                                            >  SRR4302547.6641664/1‑94 (MQ=60)

                   AGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGC                                                                            <  SRR4302547.3785597/94‑1 (MQ=60)

                   AGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGC                                                                            >  SRR4302547.2851887/1‑94 (MQ=60)

                     CGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCG                                                                          <  SRR4302547.2834104/94‑1 (MQ=60)

                     CGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCG                                                                          <  SRR4302547.540812/94‑1 (MQ=60)

                      GACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGC                                                                         >  SRR4302547.6097283/1‑94 (MQ=60)

                       ACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCT                                                                        >  SRR4302547.3344345/1‑94 (MQ=60)

                       ACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCT                                                                        <  SRR4302547.4427373/94‑1 (MQ=60)

                        CGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTG                                                                       >  SRR4302547.204608/1‑94 (MQ=60)

                         GTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGG                                                                      >  SRR4302547.6594211/1‑94 (MQ=60)

                           GGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCG                                                                    <  SRR4302547.4322273/94‑1 (MQ=60)

                            GCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGT                                                                   >  SRR4302547.1825804/1‑94 (MQ=60)

                            GCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGT                                                                   <  SRR4302547.3289672/94‑1 (MQ=60)

                              ATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTG                                                                 >  SRR4302547.4048331/1‑94 (MQ=60)

                              ATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTG                                                                 >  SRR4302547.1257186/1‑94 (MQ=60)

                               TCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGG                                                                <  SRR4302547.2776438/94‑1 (MQ=60)

                                CGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGA                                                               >  SRR4302547.3598489/1‑94 (MQ=60)

                                CGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGA                                                               <  SRR4302547.1538056/94‑1 (MQ=60)

                                CGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGA                                                               >  SRR4302547.1750202/1‑94 (MQ=60)

                                 GGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAG                                                              <  SRR4302547.4923733/94‑1 (MQ=60)

                                  GACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGTGTTGGAGG                                                             <  SRR4302547.810714/94‑1 (MQ=60)

                                   ACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGT                                                            <  SRR4302547.2417030/94‑1 (MQ=60)

                                   ACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGT                                                            <  SRR4302547.370594/94‑1 (MQ=60)

                                    CATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTG                                                           <  SRR4302547.4507153/94‑1 (MQ=60)

                                    CATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTG                                                           >  SRR4302547.3590021/1‑94 (MQ=60)

                                     ATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGG                                                          >  SRR4302547.5298166/1‑94 (MQ=60)

                                     ATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGG                                                          >  SRR4302547.1415401/1‑94 (MQ=60)

                                       ATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTC                                                        >  SRR4302547.144611/1‑94 (MQ=60)

                                       ATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTC                                                        >  SRR4302547.2813337/1‑94 (MQ=60)

                                        TCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCA                                                       >  SRR4302547.3813213/1‑94 (MQ=60)

                                        TCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCA                                                       >  SRR4302547.1548482/1‑94 (MQ=60)

                                         CCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCAT                                                      >  SRR4302547.2366247/1‑94 (MQ=60)

                                          CAGCTGGTNGNTATTGGCAATCTTNCNGTNACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGACATG                                                     <  SRR4302547.1174197/94‑1 (MQ=60)

                                          CAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATG                                                     >  SRR4302547.5311244/1‑94 (MQ=60)

                                          CAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATG                                                     >  SRR4302547.2133059/1‑94 (MQ=60)

                                           AGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGT                                                    >  SRR4302547.5855199/1‑94 (MQ=60)

                                           AGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGT                                                    >  SRR4302547.4445228/1‑94 (MQ=60)

                                           AGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGT                                                    >  SRR4302547.3881805/1‑94 (MQ=60)

                                            GCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTC                                                   <  SRR4302547.4778944/94‑1 (MQ=60)

                                             CTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCG                                                  >  SRR4302547.2736109/1‑94 (MQ=60)

                                             CTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCG                                                  <  SRR4302547.3462775/94‑1 (MQ=60)

                                             CTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCG                                                  <  SRR4302547.2541305/94‑1 (MQ=60)

                                             CTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCG                                                  <  SRR4302547.1726138/94‑1 (MQ=60)

                                             CTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCG                                                  <  SRR4302547.4454574/94‑1 (MQ=60)

                                             CTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCG                                                  <  SRR4302547.4392014/94‑1 (MQ=60)

                                              TGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGA                                                 >  SRR4302547.166807/1‑94 (MQ=60)

                                              TGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGA                                                 <  SRR4302547.4340553/94‑1 (MQ=60)

                                              TGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGA                                                 >  SRR4302547.6717596/1‑94 (MQ=60)

                                               GGTAGATATTGGCAATCTTGCCGTTACGCCCGCCGTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGAT                                                <  SRR4302547.3271762/94‑1 (MQ=60)

                                               GGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGAT                                                >  SRR4302547.2140447/1‑94 (MQ=60)

                                               GGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGAT                                                >  SRR4302547.6021342/1‑94 (MQ=60)

                                               GGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCCTTCAGTTGACGAATGACAAAGATGCAGCTGGCGATGGAGGTTGTCATTGCGAT                                                >  SRR4302547.3664331/1‑94 (MQ=60)

                                                GTAGATATTGGCTATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATG                                               >  SRR4302547.5115571/1‑94 (MQ=60)

                                                GTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATG                                               <  SRR4302547.3238697/94‑1 (MQ=60)

                                                  AGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCC                                             >  SRR4302547.4795100/1‑94 (MQ=60)

                                                   GATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCT                                            <  SRR4302547.4416218/94‑1 (MQ=60)

                                                    ATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGCCATGTCGATGCCTT                                           >  SRR4302547.1838856/1‑94 (MQ=60)

                                                     TATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTC                                          <  SRR4302547.1227659/94‑1 (MQ=60)

                                                      ATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCA                                         >  SRR4302547.5091806/1‑94 (MQ=60)

                                                      ATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCA                                         <  SRR4302547.6292341/94‑1 (MQ=60)

                                                      ATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCA                                         >  SRR4302547.662244/1‑94 (MQ=60)

                                                      ATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCA                                         >  SRR4302547.790611/1‑94 (MQ=60)

                                                       TTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAG                                        <  SRR4302547.4410882/94‑1 (MQ=60)

                                                       TTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAG                                        <  SRR4302547.4876709/94‑1 (MQ=60)

                                                       TTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAG                                        >  SRR4302547.2705166/1‑94 (MQ=60)

                                                        TGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGC                                       >  SRR4302547.4533756/1‑94 (MQ=60)

                                                          GCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCAT                                     <  SRR4302547.3513273/94‑1 (MQ=60)

                                                          GCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCAT                                     >  SRR4302547.3733227/1‑94 (MQ=60)

                                                          GCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCAT                                     >  SRR4302547.1657089/1‑94 (MQ=60)

                                                           CAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATT                                    <  SRR4302547.2728912/94‑1 (MQ=60)

                                                            AATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTG                                   >  SRR4302547.955139/1‑94 (MQ=60)

                                                            AATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTG                                   <  SRR4302547.4101593/94‑1 (MQ=60)

                                                              TCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCC                                 >  SRR4302547.4634269/1‑94 (MQ=60)

                                                              TCTTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCC                                 <  SRR4302547.375509/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               <  SRR4302547.3534723/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               <  SRR4302547.547894/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               <  SRR4302547.5441160/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               <  SRR4302547.1621738/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               <  SRR4302547.4146636/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               <  SRR4302547.4661193/94‑1 (MQ=60)

                                                                TTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCA                               >  SRR4302547.5478789/1‑94 (MQ=60)

                                                                  GCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGG                             <  SRR4302547.2672799/94‑1 (MQ=60)

                                                                  GCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGG                             <  SRR4302547.4408005/94‑1 (MQ=60)

                                                                  GCCGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGG                             <  SRR4302547.235696/94‑1 (MQ=60)

                                                                    CGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGC                           >  SRR4302547.129765/1‑94 (MQ=60)

                                                                    CGTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGC                           >  SRR4302547.817448/1‑94 (MQ=60)

                                                                     GTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCT                          >  SRR4302547.1789813/1‑94 (MQ=60)

                                                                     GTTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCT                          >  SRR4302547.6374639/1‑94 (MQ=60)

                                                                      TTACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTT                         >  SRR4302547.756973/1‑94 (MQ=60)

                                                                       TACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTC                        >  SRR4302547.752772/1‑94 (MQ=60)

                                                                       TACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTC                        <  SRR4302547.4610581/94‑1 (MQ=60)

                                                                       TACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTC                        >  SRR4302547.6731102/1‑94 (MQ=60)

                                                                        ACGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCA                       <  SRR4302547.2757712/94‑1 (MQ=60)

                                                                        ACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCA                       <  SRR4302547.5044024/94‑1 (MQ=60)

                                                                         CGCCCGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAG                      <  SRR4302547.5528779/94‑1 (MQ=60)

                                                                            CCGCCCTCGCCTTTCAGTGGAAGAATGACAAAGATGCCGCTGGTGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCA                   <  SRR4302547.6689990/94‑1 (MQ=60)

                                                                             CGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAG                  <  SRR4302547.1202615/94‑1 (MQ=60)

                                                                             CGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAG                  <  SRR4302547.187803/94‑1 (MQ=60)

                                                                             CGCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAG                  <  SRR4302547.2371365/94‑1 (MQ=60)

                                                                              GCCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGG                 <  SRR4302547.261153/94‑1 (MQ=60)

                                                                               CCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGG                <  SRR4302547.6289929/94‑1 (MQ=60)

                                                                               CCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGG                >  SRR4302547.5319660/1‑94 (MQ=60)

                                                                               CCCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGG                >  SRR4302547.3881311/1‑94 (MQ=60)

                                                                                CCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGT               >  SRR4302547.3879017/1‑94 (MQ=60)

                                                                                CCTCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGT               >  SRR4302547.259331/1‑94 (MQ=60)

                                                                                  TCGCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCA             >  SRR4302547.2447351/1‑94 (MQ=60)

                                                                                    GCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATG           >  SRR4302547.4616970/1‑94 (MQ=60)

                                                                                    GCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATG           <  SRR4302547.1928480/94‑1 (MQ=60)

                                                                                    GCCTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATG           >  SRR4302547.3296864/1‑94 (MQ=60)

                                                                                      CTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCC         <  SRR4302547.1749421/94‑1 (MQ=60)

                                                                                      CTTTCAGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCC         >  SRR4302547.4434788/1‑94 (MQ=60)

                                                                                      CTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCC         >  SRR4302547.3404461/1‑94 (MQ=60)

                                                                                           AGTGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGCTAA    >  SRR4302547.6152187/1‑94 (MQ=60)

                                                                                             GGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGCTAAAG  <  SRR4302547.2348189/94‑1 (MQ=60)

GAATTCTGTCGCCATCACCAGCGACGTGGCATCGGACATATCCAGCTGGTAGATATTGGCAATCTTGCCGTTACGCCCGCCCTCGCCTTTCAGCGGACGAATGACAAAGATGCCGCTGGCGTTGGAGGTGGTCATGTCGATGCCTTCAGCATTGCCCAGGGCTTCAGTCAGGGTCATGCCGCTAAAG  >  NC_000913/2134264‑2134450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`