Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,179,549 T→C L145P (CTG→CCG)  b2100 → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,179,5490TC86.2% 609.6 / 74.8 232L145P (CTG→CCG) b2100putative kinase
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base C (95/105);  total (107/125)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 240 total aligned reads displayed.

GGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA  >  NC_000913/2179457‑2179638
                                                                                            |                                                                                         
  aTGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGc                                                                                         <  2:4210960/93‑1 (MQ=255)
   tGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGct                                                                                        <  1:3949341/93‑1 (MQ=255)
   tGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGct                                                                                        <  1:2673686/93‑1 (MQ=255)
    ggCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGctc                                                                                       <  2:1975229/93‑1 (MQ=255)
    ggCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGctc                                                                                       <  2:1058578/93‑1 (MQ=255)
       gAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTAt                                                                                    >  1:3427898/1‑93 (MQ=255)
       gAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCTCTCTAt                                                                                    >  2:4625657/1‑93 (MQ=255)
        aaCGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAtt                                                                                   >  2:4098813/1‑93 (MQ=255)
          cGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAtttt                                                                                 <  1:1007615/93‑1 (MQ=255)
           gCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAttttt                                                                                >  1:3338334/1‑93 (MQ=255)
             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTcc                                                                              <  1:218891/93‑1 (MQ=255)
             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTAttttttc                                                                              >  2:5986183/1‑91 (MQ=255)
             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTcc                                                                              >  2:4462244/1‑93 (MQ=255)
             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTcc                                                                              <  1:6407990/93‑1 (MQ=255)
             aCTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTcc                                                                              <  2:1092723/93‑1 (MQ=255)
              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCg                                                                             <  1:1538062/93‑1 (MQ=255)
              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCg                                                                             >  2:5270697/1‑93 (MQ=255)
              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCg                                                                             >  2:2506264/1‑93 (MQ=255)
              cTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCg                                                                             >  2:3498062/1‑93 (MQ=255)
               ttttATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCgg                                                                            >  1:3546878/1‑93 (MQ=255)
                tttATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGt                                                                           >  2:4963383/1‑93 (MQ=255)
                   aTGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTAt                                                                        <  2:1505542/93‑1 (MQ=255)
                   aTGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTAt                                                                        <  2:1952829/93‑1 (MQ=255)
                   aTGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTAt                                                                        <  2:3145823/93‑1 (MQ=255)
                     gTCATTCAGCGGTGTTGAATATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCa                                                                      >  1:6481326/1‑93 (MQ=255)
                     gTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCa                                                                      >  1:1391897/1‑93 (MQ=255)
                       cATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAc                                                                    <  2:4058022/93‑1 (MQ=255)
                       cATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAc                                                                    >  1:3142636/1‑93 (MQ=255)
                        aTTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACt                                                                   <  1:3773833/93‑1 (MQ=255)
                        aTTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACt                                                                   <  1:4940706/93‑1 (MQ=255)
                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  <  1:5929680/93‑1 (MQ=255)
                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  <  1:4821347/93‑1 (MQ=255)
                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  >  2:1534149/1‑93 (MQ=255)
                         ttCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTg                                                                  >  2:4783965/1‑93 (MQ=255)
                            aGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGcc                                                               >  2:3794495/1‑93 (MQ=255)
                             gCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCt                                                              >  1:787570/1‑93 (MQ=255)
                             gCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCt                                                              <  1:1343700/93‑1 (MQ=255)
                               ggTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGGGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCg                                                            >  2:5409446/1‑93 (MQ=255)
                               ggTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCg                                                            <  2:3311328/93‑1 (MQ=255)
                               ggTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCg                                                            <  1:6336033/93‑1 (MQ=255)
                                gtgtTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGc                                                           <  1:6293703/93‑1 (MQ=255)
                                  gtTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGccc                                                         <  2:6197627/93‑1 (MQ=255)
                                  gtTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGccc                                                         <  2:2862229/93‑1 (MQ=255)
                                      aaaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcg                                                     <  2:3399709/93‑1 (MQ=255)
                                      aaaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcg                                                     >  2:6495710/1‑93 (MQ=255)
                                       aaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcgg                                                    >  2:87796/1‑93 (MQ=255)
                                       aaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcgg                                                    <  2:616276/93‑1 (MQ=255)
                                       aaaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcgg                                                    >  1:5238071/1‑93 (MQ=255)
                                        aaTCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTACGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTgcggc                                                   >  1:541114/1‑93 (MQ=255)
                                           cAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGaa                                                <  1:6643011/93‑1 (MQ=255)
                                           cAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGaa                                                >  1:3301428/1‑93 (MQ=255)
                                            aGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAAt                                               <  2:5335085/93‑1 (MQ=255)
                                               ggAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGt                                            >  2:1025400/1‑93 (MQ=255)
                                                gAATCGCCAGTGGCTGGCGGGATTAACCGTTGCGCCTGGCAACCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGtt                                           <  2:1934994/93‑1 (MQ=255)
                                                gAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGtt                                           <  2:1325957/93‑1 (MQ=255)
                                                gAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGtt                                           <  2:6303174/93‑1 (MQ=255)
                                                 aaTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTa                                          >  1:4161198/1‑93 (MQ=255)
                                                  aTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAg                                         <  1:5980039/93‑1 (MQ=255)
                                                  aTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAg                                         >  2:2465418/1‑93 (MQ=255)
                                                  aTCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAg                                         <  1:6376765/93‑1 (MQ=255)
                                                   tCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGt                                        <  2:1875696/93‑1 (MQ=255)
                                                   tCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGt                                        <  1:2080745/93‑1 (MQ=255)
                                                   tCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGt                                        <  2:1747411/93‑1 (MQ=255)
                                                    cGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTg                                       <  2:5849316/93‑1 (MQ=255)
                                                    cGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTg                                       >  2:1805032/1‑93 (MQ=255)
                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  2:771235/93‑1 (MQ=255)
                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  1:5723971/93‑1 (MQ=255)
                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  2:576491/93‑1 (MQ=255)
                                                        aGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAt                                   <  2:4758143/93‑1 (MQ=255)
                                                         gTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATg                                  >  2:5708482/1‑93 (MQ=255)
                                                             ctggcGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTg                              >  2:3033375/1‑93 (MQ=255)
                                                              gggcGCTATTCCCCGTTGCGTCTGGCAGCC‑GCTCTATTTTTCGGTTTATCACCT‑GCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGaa                           <  1:466746/92‑1 (MQ=255)
                                                               ggcGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGa                            >  2:2137586/1‑93 (MQ=255)
                                                                gcgcGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGaa                           <  1:2970940/93‑1 (MQ=255)
                                                                  gcgATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAaga                         <  1:2443026/93‑1 (MQ=255)
                                                                    gATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGc                       >  2:5548030/1‑93 (MQ=255)
                                                                    gATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGc                       >  2:1270077/1‑93 (MQ=255)
                                                                     atgaaCCGTTGCGCCGGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCt                      <  1:4200370/90‑1 (MQ=255)
                                                                     aTTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCt                      >  1:513985/1‑93 (MQ=255)
                                                                       tAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGc                    <  1:3090040/93‑1 (MQ=255)
                                                                        aaCCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTgga                   >  1:2926396/1‑91 (MQ=255)
                                                                        aaCCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCa                   <  1:2463110/93‑1 (MQ=255)
                                                                           cGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGa                <  1:4692438/93‑1 (MQ=255)
                                                                            gTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAc               >  2:5123940/1‑93 (MQ=255)
                                                                            gTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAc               <  1:6078906/93‑1 (MQ=255)
                                                                            gTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAc               >  1:1965164/1‑93 (MQ=255)
                                                                             ttGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACg              <  1:5367683/93‑1 (MQ=255)
                                                                                cgcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGa           >  1:4957140/1‑93 (MQ=255)
                                                                                cgcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGa           <  1:4313787/93‑1 (MQ=255)
                                                                                 gcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAc          <  2:2707554/93‑1 (MQ=255)
                                                                                 gcCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAc          <  2:5082717/93‑1 (MQ=255)
                                                                                   cTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGGATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGc        >  2:26434/1‑93 (MQ=255)
                                                                                    tGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGcc       <  1:5553977/93‑1 (MQ=255)
                                                                                     ggCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCg      <  1:2908907/93‑1 (MQ=255)
                                                                                     ggCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCg      <  1:4740867/93‑1 (MQ=255)
                                                                                      gCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGt     >  2:2328919/1‑93 (MQ=255)
                                                                                        aGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGttt   >  2:3836314/1‑93 (MQ=255)
                                                                                        aGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAAGCCGttt   >  1:676929/1‑93 (MQ=255)
                                                                                         gCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTa  >  2:6477224/1‑93 (MQ=255)
                                                                                         gCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTa  >  1:3488869/1‑93 (MQ=255)
                                                                                            |                                                                                         
GGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA  >  NC_000913/2179457‑2179638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTATCGA  >  NC_000913/2179456‑2179642
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CGGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCT                                                                                               >  SRR4302547.4835664/1‑94 (MQ=60)
CGGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCC                                                                                               <  SRR4302547.4171269/94‑1 (MQ=60)
CGGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCC                                                                                               >  SRR4302547.5416289/1‑94 (MQ=60)
 GGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCG                                                                                              <  SRR4302547.3574570/94‑1 (MQ=60)
  GATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGC                                                                                             >  SRR4302547.2505563/1‑94 (MQ=60)
    TGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTC                                                                                           <  SRR4302547.4029676/94‑1 (MQ=60)
    TGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTC                                                                                           <  SRR4302547.2727393/94‑1 (MQ=60)
      GCAAACGCACTTTTATGCCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGGAGCCTGCTCTA                                                                                         <  SRR4302547.3439608/94‑1 (MQ=60)
       CGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTAT                                                                                        >  SRR4302547.3497724/1‑94 (MQ=60)
         AACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTT                                                                                      >  SRR4302547.289171/1‑94 (MQ=60)
          ACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTT                                                                                     >  SRR4302547.2980074/1‑94 (MQ=60)
           CGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTT                                                                                    >  SRR4302547.3405882/1‑94 (MQ=60)
           CGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTT                                                                                    <  SRR4302547.1027290/94‑1 (MQ=60)
             CACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCC                                                                                  >  SRR4302547.4635870/1‑94 (MQ=60)
              ACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGGCCGCTCTATTTTTTCG                                                                                 >  SRR4302547.3621277/1‑94 (MQ=60)
              ACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCG                                                                                 <  SRR4302547.223013/94‑1 (MQ=60)
              ACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCG                                                                                 <  SRR4302547.6541623/94‑1 (MQ=60)
               CTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGG                                                                                <  SRR4302547.1568675/94‑1 (MQ=60)
               CTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGG                                                                                >  SRR4302547.3619265/1‑94 (MQ=60)
                  TTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTA                                                                             <  SRR4302547.5047064/94‑1 (MQ=60)
                     TGTCATTCAGCGGTGTTGAATATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCA                                                                          >  SRR4302547.6616264/1‑94 (MQ=60)
                     TGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTCTTTTTCCGGTTATCA                                                                          >  SRR4302547.2072796/1‑94 (MQ=60)
                     TGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCA                                                                          >  SRR4302547.1419753/1‑94 (MQ=60)
                      GTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAA                                                                         <  SRR4302547.5405123/94‑1 (MQ=60)
                       TCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAC                                                                        <  SRR4302547.3531570/94‑1 (MQ=60)
                       TCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAC                                                                        >  SRR4302547.3205067/1‑94 (MQ=60)
                       TCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAAC                                                                        >  SRR4302547.88271/1‑94 (MQ=60)
                       TCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGGTATCAAC                                                                        >  SRR4302547.3517336/1‑94 (MQ=60)
                        CATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACT                                                                       <  SRR4302547.5822663/94‑1 (MQ=60)
                        CATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACT                                                                       <  SRR4302547.6514636/94‑1 (MQ=60)
                        CATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACT                                                                       <  SRR4302547.3070125/94‑1 (MQ=60)
                         ATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTG                                                                      <  SRR4302547.3850802/94‑1 (MQ=60)
                         ATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTG                                                                      <  SRR4302547.3649137/94‑1 (MQ=60)
                         ATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTG                                                                      <  SRR4302547.5043025/94‑1 (MQ=60)
                          TTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGG                                                                     <  SRR4302547.6052613/94‑1 (MQ=60)
                          TTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGG                                                                     >  SRR4302547.3990647/1‑94 (MQ=60)
                          TTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGG                                                                     <  SRR4302547.4921263/94‑1 (MQ=60)
                           TCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGC                                                                    <  SRR4302547.190162/94‑1 (MQ=60)
                            CAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCC                                                                   >  SRR4302547.6759391/1‑94 (MQ=60)
                             AGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCT                                                                  >  SRR4302547.802479/1‑94 (MQ=60)
                              GCGTTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTC                                                                 <  SRR4302547.655713/94‑1 (MQ=60)
                              GCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTC                                                                 <  SRR4302547.1370691/94‑1 (MQ=60)
                                GGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGC                                                               <  SRR4302547.6467959/94‑1 (MQ=60)
                                GGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCCTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGTCCTCGC                                                               >  SRR4302547.6126111/1‑94 (MQ=60)
                                 GTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCC                                                              <  SRR4302547.6424536/94‑1 (MQ=60)
                                 GTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCC                                                              >  SRR4302547.2379741/1‑94 (MQ=60)
                                  TGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCC                                                             >  SRR4302547.3351456/1‑94 (MQ=60)
                                   GTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCT                                                            <  SRR4302547.4770179/94‑1 (MQ=60)
                                   GTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCT                                                            <  SRR4302547.5059487/94‑1 (MQ=60)
                                     TGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGC                                                          <  SRR4302547.6719482/94‑1 (MQ=60)
                                       AAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGG                                                        >  SRR4302547.5345903/1‑94 (MQ=60)
                                       AAAATCAGGGGAATCGCCAGTGGCTGGCGCGCTTAAACGTTGCGCCTGGCAGCCCGCTCTTTTTTTCCGGTTTTCAACTGGCGTCGACCTGCGG                                                        >  SRR4302547.1521769/1‑94 (MQ=60)
                                        AAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTTCGGTTATCAACTGGGCTCGCCCTGCGGG                                                       >  SRR4302547.217105/1‑94 (MQ=60)
                                        AAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTACGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGC                                                       >  SRR4302547.550884/1‑94 (MQ=60)
                                         AATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCG                                                      >  SRR4302547.5876429/1‑94 (MQ=60)
                                         AATCAGTGAAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCG                                                      <  SRR4302547.3028988/94‑1 (MQ=60)
                                          ATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGGCCGCTCTATTTTTCCGGTTATCAACTTGCCTCGCCCTGCGGCGA                                                     >  SRR4302547.4365669/1‑94 (MQ=60)
                                          ATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGA                                                     <  SRR4302547.5518528/94‑1 (MQ=60)
                                           TCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAA                                                    >  SRR4302547.3367881/1‑94 (MQ=60)
                                            CAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAAT                                                   <  SRR4302547.4268622/94‑1 (MQ=60)
                                            CAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAAT                                                   <  SRR4302547.6781311/94‑1 (MQ=60)
                                             AGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATT                                                  <  SRR4302547.1431770/94‑1 (MQ=60)
                                               TGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGT                                                >  SRR4302547.1508119/1‑94 (MQ=60)
                                                 GAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGGATTGTTA                                              >  SRR4302547.1285072/1‑94 (MQ=60)
                                                 GAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTA                                              >  SRR4302547.4245973/1‑94 (MQ=60)
                                                  AATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAG                                             <  SRR4302547.6055552/94‑1 (MQ=60)
                                                   ATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGT                                            <  SRR4302547.6104084/94‑1 (MQ=60)
                                                   ATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGT                                            <  SRR4302547.6509828/94‑1 (MQ=60)
                                                    TCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTG                                           <  SRR4302547.6521615/94‑1 (MQ=60)
                                                    TCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTG                                           <  SRR4302547.2122238/94‑1 (MQ=60)
                                                      GCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGA                                         <  SRR4302547.6287380/94‑1 (MQ=60)
                                                       CCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAA                                        <  SRR4302547.677418/94‑1 (MQ=60)
                                                        CAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAAT                                       <  SRR4302547.4950445/94‑1 (MQ=60)
                                                         AGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATG                                      <  SRR4302547.5842208/94‑1 (MQ=60)
                                                         AGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATG                                      <  SRR4302547.5314777/94‑1 (MQ=60)
                                                           TGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGGATGGC                                    >  SRR4302547.3593096/1‑94 (MQ=60)
                                                            GGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTGTTCCGGTTATCAGCTTGCCTCGCCGTGCGGCGAATTGTTAGTGGGATGGAT                                   <  SRR4302547.531507/94‑1 (MQ=60)
                                                             GCTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTG                                  <  SRR4302547.6451032/94‑1 (MQ=60)
                                                              CTGGCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGG                                 <  SRR4302547.4887385/94‑1 (MQ=60)
                                                               gggcgctattccCCGTTGCGTCTGGCAGCC‑GCTCTATTTTTCGGTTTATCACCT‑GCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAG                              <  SRR4302547.475345/82‑1 (MQ=56)
                                                                 GCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAG                              <  SRR4302547.3030073/94‑1 (MQ=60)
                                                                 GCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAG                              <  SRR4302547.5591074/94‑1 (MQ=60)
                                                                 GCGCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAG                              <  SRR4302547.2942828/94‑1 (MQ=60)
                                                                   GCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAG                            <  SRR4302547.2004232/94‑1 (MQ=60)
                                                                   GCGATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAG                            <  SRR4302547.2492438/94‑1 (MQ=60)
                                                                     GATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCT                          <  SRR4302547.4268087/94‑1 (MQ=60)
                                                                     GATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCT                          <  SRR4302547.4233962/94‑1 (MQ=60)
                                                                     GATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCT                          >  SRR4302547.523315/1‑94 (MQ=60)
                                                                     GATTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTCGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCC                          >  SRR4302547.6387923/1‑94 (MQ=60)
                                                                      ATGAACCGTTGCGCCGGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTG                         <  SRR4302547.4286050/94‑1 (MQ=60)
                                                                       TTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGC                        >  SRR4302547.767452/1‑94 (MQ=60)
                                                                       TTAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGC                        >  SRR4302547.6102558/1‑94 (MQ=60)
                                                                        TAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGGA                       >  SRR4302547.2984761/1‑94 (MQ=60)
                                                                        TAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCA                       <  SRR4302547.2501744/94‑1 (MQ=60)
                                                                        TAACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCA                       <  SRR4302547.3151329/94‑1 (MQ=60)
                                                                         AACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAA                      <  SRR4302547.2512896/94‑1 (MQ=60)
                                                                          ACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAG                     <  SRR4302547.3175839/94‑1 (MQ=60)
                                                                          ACCGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAG                     <  SRR4302547.3086525/94‑1 (MQ=60)
                                                                            CGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAC                   <  SRR4302547.4789619/94‑1 (MQ=60)
                                                                            CGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAC                   >  SRR4302547.2004186/1‑94 (MQ=60)
                                                                            CGTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGAC                   >  SRR4302547.645353/1‑94 (MQ=60)
                                                                             GTTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACG                  <  SRR4302547.6205008/94‑1 (MQ=60)
                                                                              TTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGT                 >  SRR4302547.4612089/1‑94 (MQ=60)
                                                                              TTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGTAAGACGT                 <  SRR4302547.5636900/94‑1 (MQ=60)
                                                                              TTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGT                 <  SRR4302547.5476617/94‑1 (MQ=60)
                                                                              TTGCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGT                 <  SRR4302547.5478456/94‑1 (MQ=60)
                                                                                GCGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGA               >  SRR4302547.5059734/1‑94 (MQ=60)
                                                                                 CGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAC              >  SRR4302547.987864/1‑94 (MQ=60)
                                                                                 CGCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAC              <  SRR4302547.4402553/94‑1 (MQ=60)
                                                                                  GCCTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACG             >  SRR4302547.2693493/1‑94 (MQ=60)
                                                                                    CTGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCC           <  SRR4302547.1408321/94‑1 (MQ=60)
                                                                                     TGGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCG          <  SRR4302547.5668552/94‑1 (MQ=60)
                                                                                      GGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGT         <  SRR4302547.2966922/94‑1 (MQ=60)
                                                                                      GGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGT         <  SRR4302547.4839122/94‑1 (MQ=60)
                                                                                      GGCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGT         >  SRR4302547.5156203/1‑94 (MQ=60)
                                                                                       GCAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTT        <  SRR4302547.3293965/94‑1 (MQ=60)
                                                                                        CAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTT       >  SRR4302547.3386347/1‑94 (MQ=60)
                                                                                        CAGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGAAGCCGTTT       >  SRR4302547.689398/1‑94 (MQ=60)
                                                                                         AGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA      <  SRR4302547.4858093/94‑1 (MQ=60)
                                                                                         AGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA      >  SRR4302547.3560062/1‑94 (MQ=60)
                                                                                         AGCCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTA      >  SRR4302547.3487388/1‑94 (MQ=60)
                                                                                           CCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTATG    >  SRR4302547.1602177/1‑94 (MQ=60)
                                                                                           CCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTATC    >  SRR4302547.3233467/1‑94 (MQ=60)
                                                                                           CCCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTATC    >  SRR4302547.5867973/1‑94 (MQ=60)
                                                                                            CCGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTATCG   >  SRR4302547.3218107/1‑94 (MQ=60)
                                                                                             CGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGTAAGACGTGACGCCGTTTATCGA  <  SRR4302547.4315653/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGGATGGCGAACGCACTTTTATGTCATTCAGCGGTGTTGAAAATCAGTGGAATCGCCAGTGGCTGGCGCGATTAACCGTTGCGCCTGGCAGCCTGCTCTATTTTTCCGGTTATCAACTGGCCTCGCCCTGCGGCGAATTGTTAGTGGAATGGCTGGAAGAGCTGCAAGACGTGACGCCGTTTATCGA  >  NC_000913/2179456‑2179642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑