Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,062	C→T	F266F (TTC→TTT)	yeiQ →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,062	0	C	T	84.7%	607.0 / 75.8	215	F266F (TTC→TTT)	yeiQ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (74/108); total (91/124)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGG  >  NC_000913/2264970‑2265154

 cACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                                                                                             <  1:1429947/93‑1 (MQ=255)

   cTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTttgtc                                                                                           >  2:3355014/1‑93 (MQ=255)

    tggtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTttgtcg                                                                                          >  2:2632690/1‑93 (MQ=255)

     ggtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGc                                                                                         <  1:4820197/93‑1 (MQ=255)

       tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTg                                                                                       <  1:5171114/93‑1 (MQ=255)

       tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTg                                                                                       <  2:3080788/93‑1 (MQ=255)

       tggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGCTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTg                                                                                       >  2:2305703/1‑93 (MQ=255)

        ggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTgg                                                                                      <  2:1259922/93‑1 (MQ=255)

        ggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTgg                                                                                      >  2:2549601/1‑93 (MQ=255)

            aTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGTAGATAACTTCGTCGCTGGGCGt                                                                                  <  2:5944878/93‑1 (MQ=255)

              cagccagtATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcc                                                                                <  2:5267182/93‑1 (MQ=255)

              cagccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGCAAGATAACTTCGTCGCTGGGCGTcc                                                                                <  1:2827336/93‑1 (MQ=255)

               agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcct                                                                               <  2:4044752/93‑1 (MQ=255)

               agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcct                                                                               <  2:6654602/93‑1 (MQ=255)

                 ccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcctgc                                                                             >  1:4390406/1‑93 (MQ=255)

                  cagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcctgcc                                                                            <  1:5773977/93‑1 (MQ=255)

                  cagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc                                                                            <  1:523877/93‑1 (MQ=255)

                   agcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTcctgcct                                                                           <  1:5992466/93‑1 (MQ=255)

                    gcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg                                                                          <  2:3938586/93‑1 (MQ=255)

                     cATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTgg                                                                         <  2:3256780/93‑1 (MQ=255)

                     cATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTCTGTCGCTGGGCGTCCTGCCTgg                                                                         <  2:4744349/93‑1 (MQ=255)

                      aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTggg                                                                        <  1:13219/93‑1 (MQ=255)

                      aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTggg                                                                        <  2:4897517/93‑1 (MQ=255)

                      aTCGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTggg                                                                        <  2:5591796/93‑1 (MQ=255)

                       tCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGa                                                                       <  1:1292805/93‑1 (MQ=255)

                       tCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGa                                                                       <  1:4889260/93‑1 (MQ=255)

                         tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAg                                                                     >  2:2533575/1‑93 (MQ=255)

                          tggggTGAATGATCCCTGCGCGATTAGCTGCGAGCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    <  2:4368506/92‑1 (MQ=255)

                          gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    <  2:1144413/93‑1 (MQ=255)

                          gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    >  1:2653689/1‑93 (MQ=255)

                          gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGt                                                                    <  2:2045854/93‑1 (MQ=255)

                           ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTc                                                                   >  2:4250670/1‑93 (MQ=255)

                           ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTc                                                                   >  2:6532961/1‑93 (MQ=255)

                               tGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAg                                                               >  1:4667666/1‑93 (MQ=255)

                               tGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAg                                                               <  1:1694177/93‑1 (MQ=255)

                                gAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAgg                                                              >  2:3740228/1‑93 (MQ=255)

                                 aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgt                                                             <  1:5095077/93‑1 (MQ=255)

                                    gATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTa                                                          <  1:1668996/93‑1 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         <  2:3610729/93‑1 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         >  1:6018741/1‑93 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         <  1:6366049/93‑1 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         >  1:5259329/1‑93 (MQ=255)

                                     aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAc                                                         >  1:3261447/1‑93 (MQ=255)

                                      tCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAGTTTGTCGCTGGGCGTCCTGCCTGGGAAGCCGCAGGTGGACa                                                        >  1:3509937/1‑93 (MQ=255)

                                      tCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACa                                                        >  2:4108338/1‑93 (MQ=255)

                                       cccTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaa                                                       >  2:1780907/1‑93 (MQ=255)

                                        ccTGCGCTATTAGCTGCGACCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa                                                      <  2:5650392/93‑1 (MQ=255)

                                         cTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAAt                                                     >  1:5548892/1‑93 (MQ=255)

                                            cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGt                                                  >  2:5312588/1‑93 (MQ=255)

                                            cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGt                                                  >  1:3164473/1‑93 (MQ=255)

                                              cgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGGACAAATGGTGa                                                >  2:5106473/1‑93 (MQ=255)

                                               gATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGaa                                               <  2:1045690/93‑1 (MQ=255)

                                               gATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGaa                                               <  2:6078831/93‑1 (MQ=255)

                                                aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAat                                              >  1:4843812/1‑93 (MQ=255)

                                                aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAat                                              >  2:1620219/1‑93 (MQ=255)

                                                aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAat                                              >  2:1848132/1‑93 (MQ=255)

                                                 ttAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatg                                             >  1:5512875/1‑93 (MQ=255)

                                                  tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatga                                            <  1:6206659/93‑1 (MQ=255)

                                                  tAGCTCCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatga                                            >  1:2807597/1‑93 (MQ=255)

                                                   aGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatgat                                           <  2:918720/93‑1 (MQ=255)

                                                    gCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAatgatg                                          <  1:2260866/93‑1 (MQ=255)

                                                       gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTcc                                       >  1:3288054/1‑93 (MQ=255)

                                                         gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTg                                     >  2:4613972/1‑93 (MQ=255)

                                                         gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTg                                     <  2:3770567/93‑1 (MQ=255)

                                                           aCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGcc                                   <  2:2855580/93‑1 (MQ=255)

                                                            ccGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCa                                  >  1:4585069/1‑93 (MQ=255)

                                                            ccGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCa                                  <  1:5624198/93‑1 (MQ=255)

                                                             cGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAt                                 >  1:6061853/1‑93 (MQ=255)

                                                             cGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAt                                 <  2:4986318/93‑1 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                >  1:3702118/1‑93 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                <  1:2380953/93‑1 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                >  1:4123039/1‑93 (MQ=255)

                                                              gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATg                                <  2:3811759/93‑1 (MQ=255)

                                                               tttATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATgg                               >  2:4529305/1‑93 (MQ=255)

                                                                ttATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATggg                              <  2:2794533/93‑1 (MQ=255)

                                                                ttATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATggg                              <  1:4308803/93‑1 (MQ=255)

                                                                 tATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGa                             <  2:6497709/93‑1 (MQ=255)

                                                                  aTCCAGTGTGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGaa                            <  1:2910100/93‑1 (MQ=255)

                                                                  aTCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGaa                            <  2:4959733/93‑1 (MQ=255)

                                                                   tCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAag                           >  2:1351356/1‑93 (MQ=255)

                                                                     cAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGCGTACAAATGGTGAATGATGTACTGCCATGGGAagag                         >  1:2937285/1‑93 (MQ=255)

                                                                      aGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAgaga                        <  1:6119149/93‑1 (MQ=255)

                                                                       gtgggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGAt                       <  2:878734/93‑1 (MQ=255)

                                                                        tgggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATg                      <  2:1771983/93‑1 (MQ=255)

                                                                        tgggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATg                      >  2:1717364/1‑93 (MQ=255)

                                                                         gggtggTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGa                     <  2:5167677/93‑1 (MQ=255)

                                                                         gggtggTGGAAGATAACTTTGTCGCGGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGa                     <  1:695807/93‑1 (MQ=255)

                                                                            tggtggAAGATAGCTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAAc                  <  2:6240/93‑1 (MQ=255)

                                                                            tggtggAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAAc                  >  1:4350163/1‑93 (MQ=255)

                                                                             ggtggAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACt                 <  1:2533218/93‑1 (MQ=255)

                                                                               tggAACATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGCGAATGATGTCCTGCCATGGGAAGAGATGAAACTGc               >  1:2229241/1‑93 (MQ=255)

                                                                                ggAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              >  1:586939/1‑93 (MQ=255)

                                                                                ggAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              >  2:4332087/1‑93 (MQ=255)

                                                                                ggAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              >  1:3979176/1‑93 (MQ=255)

                                                                                ggAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCg              <  1:1078402/93‑1 (MQ=255)

                                                                                  aaGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGa            <  2:6629375/93‑1 (MQ=255)

                                                                                       aaCTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCtt       <  1:830781/93‑1 (MQ=255)

                                                                                       aaCTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCtt       >  2:5775481/1‑93 (MQ=255)

                                                                                           tcgtcgCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATg   <  1:5899798/93‑1 (MQ=255)

                                                                                            cgtcgCTGGGGGTGCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATgg  <  1:1884051/93‑1 (MQ=255)

TCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGG  >  NC_000913/2264970‑2265154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGC  >  NC_000913/2264969‑2265155

ATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTT                                                                                               >  SRR4302547.4783894/1‑94 (MQ=60)

ATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTC                                                                                               <  SRR4302547.4333512/94‑1 (MQ=60)

  CACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGT                                                                                             <  SRR4302547.1458546/94‑1 (MQ=60)

   ACTGGTGGAAATCAGCCAGCATCTGGGGGTGCATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTC                                                                                            >  SRR4302547.3278509/1‑94 (MQ=60)

   ACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAGCCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTC                                                                                            <  SRR4302547.615495/94‑1 (MQ=60)

   ACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTC                                                                                            <  SRR4302547.3820730/94‑1 (MQ=60)

   ACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTC                                                                                            <  SRR4302547.2925546/94‑1 (MQ=60)

      GGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCT                                                                                         <  SRR4302547.4920089/94‑1 (MQ=60)

      GGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCT                                                                                         <  SRR4302547.5749049/94‑1 (MQ=60)

      GGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCT                                                                                         >  SRR4302547.1332347/1‑94 (MQ=60)

        TGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGG                                                                                       <  SRR4302547.1733046/94‑1 (MQ=60)

        TGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGG                                                                                       <  SRR4302547.5277575/94‑1 (MQ=60)

          GAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGC                                                                                     <  SRR4302547.2985613/94‑1 (MQ=60)

            AATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGT                                                                                   <  SRR4302547.3076908/94‑1 (MQ=60)

            AATCAGCCAGCATCTGGGGGTGAATGAGCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGT                                                                                   <  SRR4302547.1348938/94‑1 (MQ=60)

               CAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCT                                                                                <  SRR4302547.2538606/94‑1 (MQ=60)

               CAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGCAAGATAACTTCGTCGCTGGGCGTCCT                                                                                <  SRR4302547.2883879/94‑1 (MQ=60)

                AGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTG                                                                               <  SRR4302547.3433785/94‑1 (MQ=60)

                 GCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGC                                                                              >  SRR4302547.2280545/1‑94 (MQ=60)

                 GCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGC                                                                              >  SRR4302547.4480750/1‑94 (MQ=60)

                 GCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGC                                                                              >  SRR4302547.6138905/1‑94 (MQ=60)

                   CAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCT                                                                            <  SRR4302547.5893400/94‑1 (MQ=60)

                   CAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCT                                                                            <  SRR4302547.490533/94‑1 (MQ=60)

                   CAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCT                                                                            <  SRR4302547.533359/94‑1 (MQ=60)

                   CAGCATCTGGGGGTGAATGATCCCTGAGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCA                                                                            <  SRR4302547.5469940/94‑1 (MQ=60)

                    AGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTG                                                                           <  SRR4302547.6116801/94‑1 (MQ=60)

                     GCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGG                                                                          >  SRR4302547.5004500/1‑94 (MQ=60)

                     GCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGG                                                                          <  SRR4302547.5203735/94‑1 (MQ=60)

                      CATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGG                                                                         <  SRR4302547.3671364/94‑1 (MQ=60)

                       ATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGA                                                                        <  SRR4302547.13467/94‑1 (MQ=60)

                       ATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGA                                                                        <  SRR4302547.2379836/94‑1 (MQ=60)

                        TCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAA                                                                       <  SRR4302547.1318829/94‑1 (MQ=60)

                        TCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAA                                                                       <  SRR4302547.5690242/94‑1 (MQ=60)

                        TCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAA                                                                       <  SRR4302547.4990525/94‑1 (MQ=60)

                        TCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAA                                                                       >  SRR4302547.6019925/1‑94 (MQ=60)

                          TGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGT                                                                     >  SRR4302547.2707031/1‑94 (MQ=60)

                          TGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGT                                                                     <  SRR4302547.5497591/94‑1 (MQ=60)

                           GGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTC                                                                    >  SRR4302547.4130063/1‑94 (MQ=60)

                            GGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCG                                                                   >  SRR4302547.2584645/1‑94 (MQ=60)

                            GGGGGGAATGATCCCTGCGCGATTAGCTGCGAACCGTGTATCCAGTGGGTGGAGGAAGATACCTTTGTCGCTAGGCGTCCTGCCTGGGAAGTCG                                                                   >  SRR4302547.432939/1‑94 (MQ=60)

                             GGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGC                                                                  >  SRR4302547.1711409/1‑94 (MQ=60)

                               GTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAG                                                                >  SRR4302547.4764331/1‑94 (MQ=60)

                               GTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAG                                                                <  SRR4302547.2867320/94‑1 (MQ=60)

                                TGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGG                                                               <  SRR4302547.1727494/94‑1 (MQ=60)

                                 GAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGG                                                              >  SRR4302547.1994733/1‑94 (MQ=60)

                                  AATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTG                                                             <  SRR4302547.5200147/94‑1 (MQ=60)

                                    TGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTA                                                           <  SRR4302547.6075267/94‑1 (MQ=60)

                                     GATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAC                                                          >  SRR4302547.6143656/1‑94 (MQ=60)

                                     GATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAC                                                          <  SRR4302547.1701887/94‑1 (MQ=60)

                                     GATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAC                                                          >  SRR4302547.3326662/1‑94 (MQ=60)

                                     GATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAC                                                          >  SRR4302547.5367631/1‑94 (MQ=60)

                                      ATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAGTTTGTCGCTGGGCGTCCTGCCTGGGAAGCCGCAGGTGGACA                                                         >  SRR4302547.3581569/1‑94 (MQ=60)

                                      ATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACA                                                         <  SRR4302547.6498859/94‑1 (MQ=60)

                                       TCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAA                                                        >  SRR4302547.6030919/1‑94 (MQ=60)

                                        CCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAA                                                       >  SRR4302547.2176500/1‑94 (MQ=60)

                                         CCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAAT                                                      >  SRR4302547.5663388/1‑94 (MQ=60)

                                            GCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGT                                                   >  SRR4302547.3227432/1‑94 (MQ=60)

                                             CGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTG                                                  <  SRR4302547.4608440/94‑1 (MQ=60)

                                             CGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTG                                                  >  SRR4302547.5312452/1‑94 (MQ=60)

                                              GCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGA                                                 >  SRR4302547.2481590/1‑94 (MQ=60)

                                               CGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGAGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGGA                                                >  SRR4302547.5884116/1‑94 (MQ=60)

                                               CGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAA                                                >  SRR4302547.4471263/1‑94 (MQ=60)

                                                GATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAAT                                               >  SRR4302547.4944191/1‑94 (MQ=60)

                                                 ATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATG                                              >  SRR4302547.5626682/1‑94 (MQ=60)

                                                 ATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATG                                              >  SRR4302547.3071960/1‑94 (MQ=60)

                                                  TTAGCTCCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGA                                             >  SRR4302547.2863785/1‑94 (MQ=60)

                                                   TAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGAT                                            <  SRR4302547.6335520/94‑1 (MQ=60)

                                                    AGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATG                                           >  SRR4302547.1579215/1‑94 (MQ=60)

                                                     GCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGT                                          <  SRR4302547.2306544/94‑1 (MQ=60)

                                                      CTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTC                                         <  SRR4302547.1144516/94‑1 (MQ=60)

                                                      CTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTC                                         <  SRR4302547.4247346/94‑1 (MQ=60)

                                                       TGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCC                                        <  SRR4302547.6416169/94‑1 (MQ=60)

                                                       TGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCC                                        >  SRR4302547.3354034/1‑94 (MQ=60)

                                                         CGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTG                                      <  SRR4302547.4399154/94‑1 (MQ=60)

                                                          GAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGC                                     >  SRR4302547.4933379/1‑94 (MQ=60)

                                                            ACCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCA                                   >  SRR4302547.4679956/1‑94 (MQ=60)

                                                             CCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAT                                  >  SRR4302547.6187593/1‑94 (MQ=60)

                                                             CCGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCAT                                  <  SRR4302547.5740257/94‑1 (MQ=60)

                                                              CGTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATG                                 >  SRR4302547.3777682/1‑94 (MQ=60)

                                                              CGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATG                                 >  SRR4302547.4206835/1‑94 (MQ=60)

                                                               GTTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGG                                <  SRR4302547.2429192/94‑1 (MQ=60)

                                                               GTTTATCAAGTGGGTGGTGGAAAATACCTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTAAAAATGGTGAATGATGTCCTGCCATGG                                <  SRR4302547.4475455/94‑1 (MQ=60)

                                                                TTTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGG                               <  SRR4302547.716149/94‑1 (MQ=60)

                                                                 TTATCCAGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGA                              >  SRR4302547.5662166/1‑94 (MQ=60)

                                                                 TTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGA                              <  SRR4302547.4397442/94‑1 (MQ=60)

                                                                   ATCCAGTGTGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAG                            <  SRR4302547.2968138/94‑1 (MQ=60)

                                                                     CCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGCGTACAAATGGTGAATGATGTACTGCCATGGGAAGAG                          >  SRR4302547.2995816/1‑94 (MQ=60)

                                                                       AGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGAT                        <  SRR4302547.5229964/94‑1 (MQ=60)

                                                                       AGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGAT                        >  SRR4302547.33415/1‑94 (MQ=60)

                                                                       AGTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGAT                        >  SRR4302547.495295/1‑94 (MQ=60)

                                                                       AGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGAT                        <  SRR4302547.6246075/94‑1 (MQ=60)

                                                                        GTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATG                       >  SRR4302547.4752891/1‑94 (MQ=60)

                                                                        GTGGGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATG                       <  SRR4302547.5350898/94‑1 (MQ=60)

                                                                          GGGTGGTGGAAGATAACTTTGTCGCGGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAA                     <  SRR4302547.708660/94‑1 (MQ=60)

                                                                           GGTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAA                    >  SRR4302547.3554085/1‑94 (MQ=60)

                                                                            GTGGTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAAC                   >  SRR4302547.4439549/1‑94 (MQ=60)

                                                                             TGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACT                  <  SRR4302547.6572470/94‑1 (MQ=60)

                                                                              GGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTG                 <  SRR4302547.2584420/94‑1 (MQ=60)

                                                                               GTGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGC                <  SRR4302547.4923709/94‑1 (MQ=60)

                                                                               GTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGC                <  SRR4302547.5939525/94‑1 (MQ=60)

                                                                               GTGGAACATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGCGAATGATGTCCTGCCATGGGAAGAGATGAAACTGC                >  SRR4302547.2274172/1‑94 (MQ=60)

                                                                                TGGAAGATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCG               >  SRR4302547.597527/1‑94 (MQ=60)

                                                                                TGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCG               >  SRR4302547.4060086/1‑94 (MQ=60)

                                                                                 GGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGG              <  SRR4302547.1099923/94‑1 (MQ=60)

                                                                                     GATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGC          >  SRR4302547.2727265/1‑94 (MQ=60)

                                                                                     GATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGC          >  SRR4302547.6252406/1‑94 (MQ=60)

                                                                                      ATAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTGCAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCT         >  SRR4302547.1518983/1‑94 (MQ=60)

                                                                                       TAACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTT        <  SRR4302547.4174322/94‑1 (MQ=60)

                                                                                        AACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTA       >  SRR4302547.6239799/1‑94 (MQ=60)

                                                                                        AACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTA       <  SRR4302547.6745749/94‑1 (MQ=60)

                                                                                        AACTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTA       <  SRR4302547.846640/94‑1 (MQ=60)

                                                                                        AACTTTGTCGCTGGGAGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTA       >  SRR4302547.6235236/1‑94 (MQ=60)

                                                                                          CTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAAT     <  SRR4302547.2883521/94‑1 (MQ=60)

                                                                                          CTTTGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAAT     >  SRR4302547.6702235/1‑94 (MQ=60)

                                                                                            TCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGG   <  SRR4302547.6021968/94‑1 (MQ=60)

                                                                                             TGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGC  >  SRR4302547.4785000/1‑94 (MQ=60)

                                                                                             CGTCGCTGGGGGTGCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGC  <  SRR4302547.1921265/94‑1 (MQ=60)

ATCACTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGGTGAATGATGTCCTGCCATGGGAAGAGATGAAACTGCGGATGCTTAATGGC  >  NC_000913/2264969‑2265155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`