Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,315,162 T→C Q896R (CAG→CGG)  rcsC ← RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,315,1620TC85.4% 623.1 / 92.7 246Q896R (CAG→CGG) rcsCRcsC
Reads supporting (aligned to +/- strand):  ref base T (21/15);  new base C (105/105);  total (126/120)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 268 total aligned reads displayed.

CCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCA  >  NC_000913/2315070‑2315251
                                                                                            |                                                                                         
cccTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCgct                                                                                           <  1:1464612/93‑1 (MQ=255)
 ccTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCgctg                                                                                          >  1:4971467/1‑93 (MQ=255)
  ctctCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACcgccgc                                                                                         >  1:4259668/1‑93 (MQ=255)
     tcGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTtc                                                                                      >  2:6498123/1‑93 (MQ=255)
     tcGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTtc                                                                                      >  2:4304144/1‑93 (MQ=255)
     tcGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTtc                                                                                      >  1:6614678/1‑93 (MQ=255)
     tcGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTtc                                                                                      <  2:2205967/93‑1 (MQ=255)
      cGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTtct                                                                                     <  1:6289331/93‑1 (MQ=255)
        gCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGGCACCGCCGCTTctct                                                                                   >  2:3037656/1‑93 (MQ=255)
        gCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTctct                                                                                   >  1:4699803/1‑93 (MQ=255)
        gCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTctct                                                                                   >  2:5858667/1‑93 (MQ=255)
          atatAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTc                                                                                 <  1:4403975/93‑1 (MQ=255)
          atatAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTc                                                                                 <  1:3861127/93‑1 (MQ=255)
          atatAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTc                                                                                 <  1:6185109/93‑1 (MQ=255)
            ataACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAg                                                                               <  1:5097913/93‑1 (MQ=255)
               aCGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCa                                                                            >  1:295538/1‑93 (MQ=255)
                 gTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAAc                                                                          >  2:362715/1‑93 (MQ=255)
                  tCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACg                                                                         >  2:3810846/1‑93 (MQ=255)
                    aGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCa                                                                       <  2:6010343/93‑1 (MQ=255)
                    aGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCa                                                                       <  1:1100041/93‑1 (MQ=255)
                    aGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCa                                                                       <  2:1942595/93‑1 (MQ=255)
                     gCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCAt                                                                      <  1:894661/93‑1 (MQ=255)
                      cGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCAtt                                                                     >  2:6144757/1‑93 (MQ=255)
                       gTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTa                                                                    >  2:5768715/1‑93 (MQ=255)
                        tCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAg                                                                   >  2:2365770/1‑93 (MQ=255)
                        tCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAg                                                                   <  1:4771368/93‑1 (MQ=255)
                        tCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAg                                                                   >  1:1540429/1‑93 (MQ=255)
                         cTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGc                                                                  >  2:696365/1‑93 (MQ=255)
                           gTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAg                                                                <  2:5673040/93‑1 (MQ=255)
                           gTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAg                                                                <  1:2684329/93‑1 (MQ=255)
                            ttttATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGt                                                               >  2:788650/1‑93 (MQ=255)
                               tATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTAc                                                            <  2:4826834/93‑1 (MQ=255)
                                 tCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTc                                                          <  2:2467656/93‑1 (MQ=255)
                                 tCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTc                                                          <  1:1012583/93‑1 (MQ=255)
                                  cacaTCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTcc                                                         <  1:1421062/93‑1 (MQ=255)
                                   acaTCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCg                                                        >  2:3277273/1‑93 (MQ=255)
                                   acaTCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCg                                                        >  2:1248007/1‑93 (MQ=255)
                                   acaTCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCg                                                        >  1:1631513/1‑93 (MQ=255)
                                      tCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGAtt                                                     >  1:5822590/1‑93 (MQ=255)
                                       ccAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTa                                                    >  2:6100661/1‑93 (MQ=255)
                                         aGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTAcc                                                  <  2:1098669/93‑1 (MQ=255)
                                          gCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCg                                                 >  2:3528763/1‑93 (MQ=255)
                                          gCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCg                                                 >  1:3701663/1‑93 (MQ=255)
                                            gTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGc                                               >  2:6622189/1‑93 (MQ=255)
                                              tACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCaa                                             >  2:5465034/1‑93 (MQ=255)
                                               aCCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAAc                                            >  1:171644/1‑93 (MQ=255)
                                                 cGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGt                                          <  2:5301178/93‑1 (MQ=255)
                                                  ggCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTc                                         >  1:5234123/1‑93 (MQ=255)
                                                  ggCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTc                                         <  1:3310375/93‑1 (MQ=255)
                                                  ggCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTc                                         >  2:5530070/1‑93 (MQ=255)
                                                   gCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCCGCAACGTCa                                        >  1:3476953/1‑93 (MQ=255)
                                                    cTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAg                                       <  2:1304803/93‑1 (MQ=255)
                                                    cTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAg                                       >  1:689323/1‑93 (MQ=255)
                                                     ttCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGt                                      >  2:6240601/1‑93 (MQ=255)
                                                      tCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTc                                     >  2:6289878/1‑93 (MQ=255)
                                                       cGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTca                                    >  2:4994742/1‑92 (MQ=255)
                                                          cAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCaa                                 <  2:3141234/93‑1 (MQ=255)
                                                            ggCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACt                               <  2:4392507/93‑1 (MQ=255)
                                                             gCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTg                              >  1:5571413/1‑93 (MQ=255)
                                                              cAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGa                             <  1:1289685/93‑1 (MQ=255)
                                                                  tGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGaa                         <  2:5121974/93‑1 (MQ=255)
                                                                  tGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGaa                         <  2:4580140/93‑1 (MQ=255)
                                                                   gTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAt                        >  2:1723968/1‑93 (MQ=255)
                                                                     ccATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgc                      >  2:4106217/1‑93 (MQ=255)
                                                                     ccATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgc                      <  2:1000963/93‑1 (MQ=255)
                                                                      cATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcg                     >  2:931519/1‑93 (MQ=255)
                                                                      cATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcg                     >  1:6372610/1‑93 (MQ=255)
                                                                       aTACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgt                    >  1:398264/1‑93 (MQ=255)
                                                                       aTACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgt                    <  1:5991833/93‑1 (MQ=255)
                                                                       aTACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgt                    >  2:5921182/1‑93 (MQ=255)
                                                                       aTACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgt                    <  2:5796953/93‑1 (MQ=255)
                                                                       aTACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgt                    <  1:3390975/93‑1 (MQ=255)
                                                                        tACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgtt                   <  1:797592/93‑1 (MQ=255)
                                                                        tACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgtt                   <  2:1832803/93‑1 (MQ=255)
                                                                        tACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgtt                   <  2:5244858/93‑1 (MQ=255)
                                                                         aCCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgttg                  <  2:1410121/93‑1 (MQ=255)
                                                                           cGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgttgcg                <  2:1547621/93‑1 (MQ=255)
                                                                           cGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgttgcg                >  1:225562/1‑93 (MQ=255)
                                                                            ggACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAtgcgttgcgt               <  1:435210/93‑1 (MQ=255)
                                                                             gACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTc              <  1:1983767/93‑1 (MQ=255)
                                                                              aCTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCa             >  1:3026438/1‑93 (MQ=255)
                                                                              aCTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCa             >  1:5121721/1‑93 (MQ=255)
                                                                               cTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCaa            >  2:3996022/1‑93 (MQ=255)
                                                                                 ccAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTAcc                                                  <  1:1892956/53‑1 (MQ=255)
                                                                                 ccAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGc          <  2:2882600/93‑1 (MQ=255)
                                                                                 ccAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGc          <  2:1963960/93‑1 (MQ=255)
                                                                                 ccAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGc          <  2:2047036/93‑1 (MQ=255)
                                                                                  cAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCg         <  2:1218287/93‑1 (MQ=255)
                                                                                  cAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTAATCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCg         >  1:3125394/1‑93 (MQ=255)
                                                                                   aGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCgg        >  2:4514346/1‑93 (MQ=255)
                                                                                   aGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCgg        >  1:6516698/1‑93 (MQ=255)
                                                                                    gatacCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGATGCGTCACGCggc       >  1:2921354/4‑92 (MQ=255)
                                                                                    gACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGt       <  1:3903202/93‑1 (MQ=255)
                                                                                    gACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGt       >  1:6035655/1‑93 (MQ=255)
                                                                                     acacCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTa      <  1:2029999/93‑1 (MQ=255)
                                                                                     acacCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTa      >  1:5673/1‑93 (MQ=255)
                                                                                      cacCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTaa     <  1:2840141/93‑1 (MQ=255)
                                                                                      cacCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTaa     <  2:272966/93‑1 (MQ=255)
                                                                                       acCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAAc    >  2:472107/1‑93 (MQ=255)
                                                                                        ccgccgCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAAcc   <  2:4091588/93‑1 (MQ=255)
                                                                                            |                                                                                         
CCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCA  >  NC_000913/2315070‑2315251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCA  >  NC_000913/2315069‑2315255
                                                                                             |                                                                                             
GCCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCC                                                                                               <  SRR4302547.4513401/94‑1 (MQ=60)
ACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCC                                                                                               <  SRR4302547.5743459/94‑1 (MQ=60)
 CCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTG                                                                                              >  SRR4302547.5074322/1‑94 (MQ=60)
 CCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTG                                                                                              <  SRR4302547.1493873/94‑1 (MQ=60)
  CCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGC                                                                                             >  SRR4302547.1781174/1‑94 (MQ=60)
  CCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGC                                                                                             <  SRR4302547.5352434/94‑1 (MQ=60)
  CCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGC                                                                                             >  SRR4302547.4346836/1‑94 (MQ=60)
   CTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCT                                                                                            >  SRR4302547.6169585/1‑94 (MQ=60)
     CTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTC                                                                                          >  SRR4302547.33571/1‑94 (MQ=60)
     CTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTC                                                                                          >  SRR4302547.6752359/1‑94 (MQ=60)
       CGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTC                                                                                        <  SRR4302547.6420023/94‑1 (MQ=60)
       CGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTC                                                                                        >  SRR4302547.896552/1‑94 (MQ=60)
        GGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCT                                                                                       >  SRR4302547.4797142/1‑94 (MQ=60)
        GGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCT                                                                                       <  SRR4302547.2981127/94‑1 (MQ=60)
        GGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCT                                                                                       <  SRR4302547.5698561/94‑1 (MQ=60)
         GCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTT                                                                                      >  SRR4302547.5814405/1‑94 (MQ=60)
           ATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGATAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCG                                                                                    >  SRR4302547.301498/1‑94 (MQ=60)
           ATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCA                                                                                    <  SRR4302547.4494691/94‑1 (MQ=60)
           ATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCA                                                                                    <  SRR4302547.3939701/94‑1 (MQ=60)
           ATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCA                                                                                    <  SRR4302547.6313488/94‑1 (MQ=60)
             ATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGC                                                                                  <  SRR4302547.5203030/94‑1 (MQ=60)
             ATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGC                                                                                  <  SRR4302547.932550/94‑1 (MQ=60)
              TAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCC                                                                                 >  SRR4302547.4475979/1‑94 (MQ=60)
              TAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCC                                                                                 <  SRR4302547.1248234/94‑1 (MQ=60)
               AACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCA                                                                                >  SRR4302547.301033/1‑94 (MQ=60)
                  GTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACG                                                                             <  SRR4302547.1962444/94‑1 (MQ=60)
                  GTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACG                                                                             >  SRR4302547.3897828/1‑94 (MQ=60)
                   TCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGC                                                                            >  SRR4302547.2017988/1‑94 (MQ=60)
                     AGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCAT                                                                          <  SRR4302547.1122132/94‑1 (MQ=60)
                      GCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATT                                                                         <  SRR4302547.911864/94‑1 (MQ=60)
                       CGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTA                                                                        <  SRR4302547.6199284/94‑1 (MQ=60)
                        GTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAG                                                                       >  SRR4302547.5155058/1‑94 (MQ=60)
                        GTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAG                                                                       >  SRR4302547.1571088/1‑94 (MQ=60)
                         TCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGC                                                                      >  SRR4302547.4130857/1‑94 (MQ=60)
                         TCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGC                                                                      <  SRR4302547.4870212/94‑1 (MQ=60)
                         TCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGC                                                                      <  SRR4302547.2041648/94‑1 (MQ=60)
                            GTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGT                                                                   <  SRR4302547.54374/94‑1 (MQ=60)
                            GTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGT                                                                   <  SRR4302547.2738222/94‑1 (MQ=60)
                            GTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGT                                                                   >  SRR4302547.1731685/1‑94 (MQ=60)
                             TTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTT                                                                  >  SRR4302547.3172940/1‑94 (MQ=60)
                             TTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTT                                                                  <  SRR4302547.5995335/94‑1 (MQ=60)
                              TTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTA                                                                 >  SRR4302547.4446284/1‑94 (MQ=60)
                              TTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTA                                                                 >  SRR4302547.5086158/1‑94 (MQ=60)
                              TTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTA                                                                 <  SRR4302547.4413978/94‑1 (MQ=60)
                              TTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCAGTAGCAGTTA                                                                 <  SRR4302547.6605352/94‑1 (MQ=60)
                                  TCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACCCCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCC                                                             <  SRR4302547.6419696/94‑1 (MQ=60)
                                  TCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCC                                                             <  SRR4302547.1032375/94‑1 (MQ=60)
                                  TCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCC                                                             <  SRR4302547.4001112/94‑1 (MQ=60)
                                   CACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCG                                                            <  SRR4302547.4067596/94‑1 (MQ=60)
                                   CACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCG                                                            >  SRR4302547.1535541/1‑94 (MQ=60)
                                   CACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCG                                                            >  SRR4302547.1663807/1‑94 (MQ=60)
                                   CACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCG                                                            <  SRR4302547.1449506/94‑1 (MQ=60)
                                      CTCCAGCGTTACCGGCTTCGGCAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATT                                                         <  SRR4302547.6746760/94‑1 (MQ=60)
                                      ATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATT                                                         >  SRR4302547.1372070/1‑94 (MQ=60)
                                      ATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATT                                                         >  SRR4302547.5943058/1‑94 (MQ=60)
                                       TCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTA                                                        >  SRR4302547.2746638/1‑94 (MQ=60)
                                        CCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTAC                                                       >  SRR4302547.884580/1‑94 (MQ=60)
                                          ctctctttccctacacgacgctcttccgatcttgacattTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCG                                                     <  SRR4302547.1930364/55‑1 (MQ=60)
                                          AGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCG                                                     >  SRR4302547.3777220/1‑94 (MQ=60)
                                           GCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGG                                                    <  SRR4302547.349870/94‑1 (MQ=60)
                                           GCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGG                                                    <  SRR4302547.4901775/94‑1 (MQ=60)
                                               TACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAAC                                                >  SRR4302547.174792/1‑94 (MQ=60)
                                                 CCGCCTTCGACAGGCAGCTGTCCATACCGGCCTCCAGCCGCCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGT                                              <  SRR4302547.3774055/94‑1 (MQ=60)
                                                  CGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTC                                             >  SRR4302547.5341867/1‑94 (MQ=60)
                                                   GGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCA                                            >  SRR4302547.2732025/1‑94 (MQ=60)
                                                   GGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCA                                            <  SRR4302547.3377125/94‑1 (MQ=60)
                                                   GGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCCGCAACGTCA                                            >  SRR4302547.3547873/1‑94 (MQ=60)
                                                    GCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAG                                           <  SRR4302547.1568848/94‑1 (MQ=60)
                                                    GCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAG                                           >  SRR4302547.702049/1‑94 (MQ=60)
                                                    GCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAG                                           >  SRR4302547.5280838/1‑94 (MQ=60)
                                                     CTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGT                                          >  SRR4302547.4737049/1‑94 (MQ=60)
                                                         GACAGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCA                                      >  SRR4302547.1104687/1‑94 (MQ=60)
                                                            AGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACT                                   <  SRR4302547.5308643/94‑1 (MQ=60)
                                                            AGGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACT                                   >  SRR4302547.5618773/1‑94 (MQ=60)
                                                             GGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTG                                  >  SRR4302547.5686298/1‑94 (MQ=60)
                                                             GGCAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTA                                  >  SRR4302547.1213926/1‑94 (MQ=60)
                                                               CAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGAC                                <  SRR4302547.1315652/94‑1 (MQ=60)
                                                               CAGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGAC                                <  SRR4302547.4772466/94‑1 (MQ=60)
                                                                AGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACG                               >  SRR4302547.5203785/1‑94 (MQ=60)
                                                                AGCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACG                               >  SRR4302547.147180/1‑94 (MQ=60)
                                                                 GCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGA                              >  SRR4302547.683319/1‑94 (MQ=60)
                                                                 GCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGA                              <  SRR4302547.5979909/94‑1 (MQ=60)
                                                                 GCTGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCCGCAACGTCAGTCCCAACTGACGA                              >  SRR4302547.6482799/1‑94 (MQ=60)
                                                                   TGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAT                            <  SRR4302547.494105/94‑1 (MQ=60)
                                                                   TGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAT                            <  SRR4302547.3112218/94‑1 (MQ=60)
                                                                   TGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAT                            <  SRR4302547.1412149/94‑1 (MQ=60)
                                                                   TGTCCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAAT                            <  SRR4302547.3208798/94‑1 (MQ=60)
                                                                      CCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCG                         <  SRR4302547.3951122/94‑1 (MQ=60)
                                                                      CCATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCG                         >  SRR4302547.6505572/1‑94 (MQ=60)
                                                                       CATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGT                        >  SRR4302547.405673/1‑94 (MQ=60)
                                                                       CATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGT                        >  SRR4302547.3544924/1‑94 (MQ=60)
                                                                        ATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTT                       <  SRR4302547.6381017/94‑1 (MQ=60)
                                                                        ATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTT                       <  SRR4302547.6116150/94‑1 (MQ=60)
                                                                        ATACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTT                       <  SRR4302547.3459955/94‑1 (MQ=60)
                                                                         TACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTG                      <  SRR4302547.812688/94‑1 (MQ=60)
                                                                         TACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTG                      >  SRR4302547.2952023/1‑94 (MQ=60)
                                                                         TACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTG                      <  SRR4302547.2967605/94‑1 (MQ=60)
                                                                         TACCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTG                      <  SRR4302547.6725169/94‑1 (MQ=60)
                                                                           CCGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCG                    >  SRR4302547.229793/1‑94 (MQ=60)
                                                                            CGGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGT                   <  SRR4302547.3774331/94‑1 (MQ=60)
                                                                             GGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTC                  <  SRR4302547.443272/94‑1 (MQ=60)
                                                                             GGACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTC                  >  SRR4302547.2713014/1‑94 (MQ=60)
                                                                              GACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCA                 >  SRR4302547.1655898/1‑94 (MQ=60)
                                                                              GACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCA                 >  SRR4302547.3086616/1‑94 (MQ=60)
                                                                              GACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCA                 <  SRR4302547.2023162/94‑1 (MQ=60)
                                                                              GACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCA                 >  SRR4302547.5227253/1‑94 (MQ=60)
                                                                               ACTCCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAA                >  SRR4302547.1651886/1‑94 (MQ=60)
                                                                                  CCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTTAAGCG             <  SRR4302547.5896071/94‑1 (MQ=60)
                                                                                  CCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCG             >  SRR4302547.741120/1‑94 (MQ=60)
                                                                                  CCAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTAATCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCG             >  SRR4302547.3187394/1‑94 (MQ=60)
                                                                                   CAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGG            >  SRR4302547.717857/1‑94 (MQ=60)
                                                                                   CAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGG            >  SRR4302547.6652370/1‑94 (MQ=60)
                                                                                   CAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGG            >  SRR4302547.3458312/1‑94 (MQ=60)
                                                                                   CAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGG            >  SRR4302547.2988109/1‑94 (MQ=60)
                                                                                   CAGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGG            <  SRR4302547.2116706/94‑1 (MQ=60)
                                                                                    AGATACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGATGCGTCACGCGGC           >  SRR4302547.2979603/1‑94 (MQ=60)
                                                                                    AGACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGT           >  SRR4302547.6160900/1‑94 (MQ=60)
                                                                                     GACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTA          >  SRR4302547.4015841/1‑94 (MQ=60)
                                                                                     GACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTA          >  SRR4302547.6156271/1‑94 (MQ=60)
                                                                                     GACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTA          >  SRR4302547.5764/1‑94 (MQ=60)
                                                                                     GACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTA          <  SRR4302547.3982575/94‑1 (MQ=60)
                                                                                     GACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTA          <  SRR4302547.394434/94‑1 (MQ=60)
                                                                                      ACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAA         <  SRR4302547.6624215/94‑1 (MQ=60)
                                                                                      ACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAA         >  SRR4302547.4850590/1‑94 (MQ=60)
                                                                                      ACACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAA         <  SRR4302547.2070424/94‑1 (MQ=60)
                                                                                       CACCGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAAC        <  SRR4302547.2896899/94‑1 (MQ=60)
                                                                                          CGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCAT     <  SRR4302547.1870429/94‑1 (MQ=60)
                                                                                          CGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCAT     >  SRR4302547.573792/1‑94 (MQ=60)
                                                                                          CGCCGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCAT     <  SRR4302547.2136360/94‑1 (MQ=60)
                                                                                             CGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCA  >  SRR4302547.3702111/1‑94 (MQ=60)
                                                                                             CGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCA  >  SRR4302547.3318456/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCA  >  NC_000913/2315069‑2315255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑