Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,359	T→C	Y85C (TAC→TGC)	yfbB ←	hyptothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,359	0	T	C	88.9%	617.2 / 51.6	225	Y85C (TAC→TGC)	yfbB	hyptothetical protein
Reads supporting (aligned to +/- strand): ref base T (14/11); new base C (105/95); total (119/106)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 230 total aligned reads displayed.

AGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCG  >  NC_000913/2375269‑2375449

aGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAc                                                                                          <  2:1488204/93‑1 (MQ=255)

aGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAc                                                                                          <  2:2292011/93‑1 (MQ=255)

aGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAACCGAGCAc                                                                                          >  2:4294004/1‑93 (MQ=255)

  cccgGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACTAAGCGAGCAccc                                                                                        <  1:5309219/93‑1 (MQ=255)

  ccccGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAccc                                                                                        >  1:4252952/1‑93 (MQ=255)

    ccGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCcca                                                                                      >  1:4220777/1‑93 (MQ=255)

      ggATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCNTCCACCAAGCGAGCACCccncc                                                                                    >  2:5214942/1‑93 (MQ=255)

      ggATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCccacc                                                                                    >  1:6324275/1‑93 (MQ=255)

      ggATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCccaca                                                                                    >  1:4240866/1‑92 (MQ=255)

        aTGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccag                                                                                  <  2:1044343/93‑1 (MQ=255)

            ccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccagccag                                                                              >  1:5058277/1‑93 (MQ=255)

            ccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccagccag                                                                              >  2:1125272/1‑93 (MQ=255)

            ccGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCAccagccag                                                                              >  1:3378014/1‑93 (MQ=255)

             cGCCTTCGACAATACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGa                                                                             <  2:5003886/93‑1 (MQ=255)

             cGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGa                                                                             >  2:362246/1‑93 (MQ=255)

              gCCTTCTAAAATACCCCCACAAAGCCCCGCCGGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGaa                                                                            <  2:5916069/93‑1 (MQ=255)

              gCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGaa                                                                            <  1:2735169/93‑1 (MQ=255)

               ccTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAg                                                                           >  2:3773439/1‑93 (MQ=255)

               ccTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAg                                                                           >  2:1373793/1‑93 (MQ=255)

                 ttCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTc                                                                         <  1:5934150/93‑1 (MQ=255)

                 ttCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTc                                                                         >  2:4485141/1‑93 (MQ=255)

                    gACAAAAACCCCAAAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAg                                                                      <  2:3269091/93‑1 (MQ=255)

                    aaCAAAACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAg                                                                      <  2:657666/92‑1 (MQ=255)

                      cAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGa                                                                    <  2:1508074/93‑1 (MQ=255)

                       aaTACCCCCACAAGCCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAt                                                                   <  1:257353/93‑1 (MQ=255)

                       aaTACCCCCACAACGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGAt                                                                   <  2:2721496/93‑1 (MQ=255)

                        aTAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtg                                                                  >  2:5468429/1‑93 (MQ=255)

                         tacccccccAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGAtgt                                                                 <  2:6016400/90‑1 (MQ=255)

                         tAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtgt                                                                 >  1:3327199/1‑93 (MQ=255)

                         aacccccACAAGGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtgt                                                                 <  2:187846/90‑1 (MQ=255)

                          acccccACAAGGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAtgtt                                                                <  1:2912881/91‑1 (MQ=255)

                             cccACAAATCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGta                                                             >  1:6481149/1‑93 (MQ=255)

                               cacaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaac                                                           <  2:577597/93‑1 (MQ=255)

                                acaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGtaact                                                          >  1:3200023/1‑93 (MQ=255)

                                acaAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaact                                                          >  2:2131104/1‑93 (MQ=255)

                                 caAGGCCCGGCGAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaacta                                                         <  2:2180707/93‑1 (MQ=255)

                                   acgCCCCGCCAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaactaac                                                       <  1:3034656/91‑1 (MQ=255)

                                   aacccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGtaactaac                                                       <  2:1710804/90‑1 (MQ=255)

                                    acccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      <  1:131655/91‑1 (MQ=255)

                                    aGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      <  2:1802049/93‑1 (MQ=255)

                                    aGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      >  2:6558966/1‑93 (MQ=255)

                                    aGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAAcc                                                      <  2:1016413/93‑1 (MQ=255)

                                      ccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCaa                                                    <  1:2367321/93‑1 (MQ=255)

                                        ccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCATGGATGTTGTAACTAACCAAgg                                                  >  1:6098487/1‑93 (MQ=255)

                                        ccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAgg                                                  >  2:3546266/1‑93 (MQ=255)

                                        ccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAgg                                                  <  1:3972525/93‑1 (MQ=255)

                                         cGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGt                                                 <  1:2317262/93‑1 (MQ=255)

                                            cAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGtttt                                              <  2:3132124/93‑1 (MQ=255)

                                            cAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGtttt                                              <  1:2540161/93‑1 (MQ=255)

                                             aGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTa                                             <  2:911087/93‑1 (MQ=255)

                                              gCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTAc                                            >  2:5403320/1‑93 (MQ=255)

                                                cccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGc                                          >  2:680588/1‑93 (MQ=255)

                                                 ccTGGCAAGCCGCCATCATCGCCGCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCa                                         <  2:4388746/93‑1 (MQ=255)

                                                  cTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAg                                        >  1:2012692/1‑93 (MQ=255)

                                                    ggCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                      >  1:1926866/1‑93 (MQ=255)

                                                    ggCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                      >  1:1401112/1‑93 (MQ=255)

                                                    gccAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTa                                      <  2:4929265/91‑1 (MQ=255)

                                                     gCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTaa                                     <  2:2896184/93‑1 (MQ=255)

                                                       aaGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGt                                   <  1:4580408/93‑1 (MQ=255)

                                                        aGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGtg                                  >  1:1059390/1‑92 (MQ=255)

                                                        aGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTc                                  <  1:6246703/93‑1 (MQ=255)

                                                             ccATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                             <  2:5908723/93‑1 (MQ=255)

                                                             ccATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                             >  2:2536569/1‑93 (MQ=255)

                                                             ccATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGa                             <  1:2649499/93‑1 (MQ=255)

                                                              catcatCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAc                            >  2:2061259/1‑93 (MQ=255)

                                                              catcatCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGCTTTACGCAGTAAGTCGGTGAc                            >  1:3921381/1‑93 (MQ=255)

                                                               atcatcGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAca                           <  1:3770208/93‑1 (MQ=255)

                                                               atcatcGCCACCCGTCCAACAAGCGAGCACCCCACCAGACAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAca                           >  1:2156149/1‑93 (MQ=255)

                                                                tcatcGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcat                          >  1:5475424/1‑93 (MQ=255)

                                                                tcatcGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcat                          <  1:4137554/93‑1 (MQ=255)

                                                                   tcGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAcatcat                       >  1:4477916/1‑93 (MQ=255)

                                                                    cGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACatcatc                      <  2:4175314/93‑1 (MQ=255)

                                                                      ccACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCaa                    <  1:6045808/93‑1 (MQ=255)

                                                                        ccccGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                  <  1:1198552/92‑1 (MQ=255)

                                                                        aCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                  >  1:5881360/1‑93 (MQ=255)

                                                                        aCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAt                  >  2:1693581/1‑93 (MQ=255)

                                                                          ccGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                <  2:3015580/93‑1 (MQ=255)

                                                                          ccGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                <  1:3685605/93‑1 (MQ=255)

                                                                          ccGGCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATcc                <  2:4444943/93‑1 (MQ=255)

                                                                           cGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCa               <  2:3084605/93‑1 (MQ=255)

                                                                           cGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCa               <  1:6185796/93‑1 (MQ=255)

                                                                            gTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAt              >  1:5552453/1‑93 (MQ=255)

                                                                             tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             <  2:6269857/93‑1 (MQ=255)

                                                                             tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             >  1:3531940/1‑93 (MQ=255)

                                                                             tCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             <  1:3986785/93‑1 (MQ=255)

                                                                             tCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATc             >  1:4852134/1‑93 (MQ=255)

                                                                               caccaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGa           >  2:4189867/1‑93 (MQ=255)

                                                                               caccaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGa           <  2:3994350/93‑1 (MQ=255)

                                                                                accaATCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTGTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          <  1:731709/93‑1 (MQ=255)

                                                                                accaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          >  1:4436310/1‑93 (MQ=255)

                                                                                accaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          >  2:162255/1‑93 (MQ=255)

                                                                                accaAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGGTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc          >  1:4098352/1‑93 (MQ=255)

                                                                                  caAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGc        <  1:6640443/93‑1 (MQ=255)

                                                                                  caAGCGACCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGc        <  1:2893352/93‑1 (MQ=255)

                                                                                    aGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTa      >  2:743151/1‑93 (MQ=255)

                                                                                    aGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTa      >  1:1572263/1‑93 (MQ=255)

                                                                                    aGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTa      >  1:5511029/1‑93 (MQ=255)

                                                                                      cGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAAt    <  1:961596/93‑1 (MQ=255)

                                                                                       gAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATc   >  2:5107377/1‑93 (MQ=255)

                                                                                        aGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATcg  >  2:2044387/1‑93 (MQ=255)

AGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCG  >  NC_000913/2375269‑2375449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCG  >  NC_000913/2375266‑2375452

TGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGC                                                                                               >  SRR4302547.5522559/1‑94 (MQ=60)

TGCAGCCCCGCATCCCAGCCTTCTACAAAACCCACACAAAGCCCCGCCAGCCCCGGGCAGGCCGCCATCATCGCCACCCGTCCACCAAGCGAGC                                                                                               <  SRR4302547.1047542/94‑1 (MQ=53)

 cgAGCCCGGGATGCCCGCCTAGGAAAATAACCCCACAAGGCCCCGCAAGCCCTTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCA                                                                                              <  SRR4302547.3276509/92‑1 (MQ=60)

 cgAGCCCCGGATGCCCGCCTTCGACAATACCCCCAAAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCA                                                                                              <  SRR4302547.2504872/92‑1 (MQ=60)

  CAGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAC                                                                                             <  SRR4302547.6178068/94‑1 (MQ=60)

  CAGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAC                                                                                             <  SRR4302547.3134153/94‑1 (MQ=60)

  CAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAC                                                                                             >  SRR4302547.4918557/1‑94 (MQ=60)

  CAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGACCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCAC                                                                                             >  SRR4302547.2180792/1‑94 (MQ=60)

   GGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACC                                                                                            <  SRR4302547.3131093/94‑1 (MQ=60)

   AGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACC                                                                                            >  SRR4302547.432539/1‑94 (MQ=60)

    gccccggactccagcttgggtcaagatCCCCACAAAGCCCCGCCAGCCCCTGGCAGTCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCC                                                                                           <  SRR4302547.3422794/67‑1 (MQ=60)

    GCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCC                                                                                           >  SRR4302547.4339971/1‑94 (MQ=60)

     CCCGGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACTAAGCGAGCACCCC                                                                                          <  SRR4302547.5418720/94‑1 (MQ=60)

      CCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCA                                                                                         <  SRR4302547.6596397/94‑1 (MQ=60)

      CCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCA                                                                                         >  SRR4302547.4306985/1‑94 (MQ=60)

      CCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCA                                                                                         >  SRR4302547.6413273/1‑94 (MQ=60)

        CGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACC                                                                                       >  SRR4302547.6455778/1‑94 (MQ=60)

        CGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACA                                                                                       >  SRR4302547.4327584/1‑94 (MQ=60)

        CGGATCCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACC                                                                                       <  SRR4302547.1360144/94‑1 (MQ=60)

         GGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCA                                                                                      >  SRR4302547.2770596/1‑94 (MQ=60)

            TGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCC                                                                                   >  SRR4302547.9987/1‑94 (MQ=60)

              CCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAG                                                                                 >  SRR4302547.3446648/1‑94 (MQ=60)

              CCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAG                                                                                 >  SRR4302547.5162669/1‑94 (MQ=60)

                CGCCTTCAACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAA                                                                               <  SRR4302547.6259457/94‑1 (MQ=60)

                 GCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAG                                                                              <  SRR4302547.2790059/94‑1 (MQ=60)

                 GCCTTCGAAAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAG                                                                              <  SRR4302547.2896258/94‑1 (MQ=60)

                  CCTTCGACAATACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGT                                                                             <  SRR4302547.3005189/94‑1 (MQ=60)

                   CTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTC                                                                            >  SRR4302547.3738341/1‑94 (MQ=60)

                    TTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCA                                                                           <  SRR4302547.6057194/94‑1 (MQ=60)

                        GCAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGA                                                                       >  SRR4302547.3888679/1‑94 (MQ=60)

                         CAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGAT                                                                      >  SRR4302547.4424940/1‑94 (MQ=60)

                          AATACCCCCACAAGCCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATG                                                                     <  SRR4302547.262174/94‑1 (MQ=60)

                          AATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATG                                                                     >  SRR4302547.6168921/1‑94 (MQ=60)

                           ATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGT                                                                    >  SRR4302547.3394441/1‑94 (MQ=60)

                           ATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGT                                                                    >  SRR4302547.1338659/1‑94 (MQ=60)

                             ACCCCCACAAGGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTG                                                                  <  SRR4302547.2970988/94‑1 (MQ=60)

                               CCCCACAAATCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTA                                                                >  SRR4302547.6616079/1‑94 (MQ=60)

                                CCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAA                                                               <  SRR4302547.5492986/94‑1 (MQ=60)

                                CCCACAAAGACCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACACGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAA                                                               >  SRR4302547.2886642/1‑94 (MQ=60)

                                  CACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTAGCCCACCAGCCAGAAGTCAAGGCTGGTGTAACT                                                             >  SRR4302547.3263821/1‑94 (MQ=60)

                                  CACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACT                                                             >  SRR4302547.3263820/1‑94 (MQ=60)

                                    aaaacCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAA                                                           <  SRR4302547.6759419/89‑1 (MQ=60)

                                    CAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAA                                                           >  SRR4302547.4543328/1‑94 (MQ=60)

                                      ACGCCCCGCCAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACC                                                         <  SRR4302547.3095018/94‑1 (MQ=60)

                                       ccCCCCGCCAGCCCCTGGCAGGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCA                                                        <  SRR4302547.4655682/92‑1 (MQ=60)

                                       AGCCCCGCCACCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCA                                                        <  SRR4302547.1905284/94‑1 (MQ=60)

                                       ACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCA                                                        <  SRR4302547.134033/94‑1 (MQ=60)

                                        GCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAA                                                       >  SRR4302547.794007/1‑94 (MQ=60)

                                         CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAG                                                      <  SRR4302547.2415305/94‑1 (MQ=60)

                                          CCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCATGGATGTTGTAACTAACCAAGG                                                     >  SRR4302547.6224997/1‑94 (MQ=60)

                                          CCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGG                                                     >  SRR4302547.2955938/1‑94 (MQ=60)

                                          CCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGG                                                     >  SRR4302547.2628529/1‑94 (MQ=60)

                                          CCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGG                                                     >  SRR4302547.2346500/1‑94 (MQ=60)

                                          CCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGG                                                     >  SRR4302547.4188855/1‑94 (MQ=60)

                                          CCCGCAAGCCCCTGGCAAGCCGCCATCATTGCCACCCGTCCACCAACCGAGCACCCCACCAGCCAGAAGTCAAGGCTGTTGTAACTctcaaagc                                                     >  SRR4302547.391310/1‑86 (MQ=60)

                                           CCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGT                                                    <  SRR4302547.4053312/94‑1 (MQ=60)

                                            CGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTT                                                   <  SRR4302547.1436570/94‑1 (MQ=60)

                                            CGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTT                                                   <  SRR4302547.2364234/94‑1 (MQ=60)

                                             GCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTT                                                  >  SRR4302547.5502899/1‑94 (MQ=60)

                                              CGAGCCCTTGCCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTACCTAACCAAGGTTTT                                                 <  SRR4302547.6041388/94‑1 (MQ=60)

                                              CCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTT                                                 <  SRR4302547.5101989/94‑1 (MQ=60)

                                               CAGCCCCGGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTA                                                <  SRR4302547.2591472/94‑1 (MQ=60)

                                                AGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTAC                                               <  SRR4302547.688870/94‑1 (MQ=60)

                                                AGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTAC                                               >  SRR4302547.4113950/1‑94 (MQ=60)

                                                AGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTAC                                               >  SRR4302547.4058358/1‑94 (MQ=60)

                                                  CCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGC                                             >  SRR4302547.2427029/1‑94 (MQ=60)

                                                   CCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCA                                            >  SRR4302547.5628106/1‑94 (MQ=60)

                                                    CCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAG                                           <  SRR4302547.2036422/94‑1 (MQ=60)

                                                    CCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAG                                           >  SRR4302547.2052731/1‑94 (MQ=60)

                                                     CTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGT                                          <  SRR4302547.4314474/94‑1 (MQ=60)

                                                      TGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTA                                         >  SRR4302547.4045762/1‑94 (MQ=60)

                                                      TGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTA                                         >  SRR4302547.1429145/1‑94 (MQ=60)

                                                      TGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTA                                         >  SRR4302547.1523218/1‑94 (MQ=60)

                                                      TGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTA                                         >  SRR4302547.1965046/1‑94 (MQ=60)

                                                       GGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAA                                        >  SRR4302547.5949513/1‑94 (MQ=60)

                                                        GCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAG                                       <  SRR4302547.4337189/94‑1 (MQ=60)

                                                         CAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGT                                      >  SRR4302547.586276/1‑94 (MQ=60)

                                                         CAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGT                                      <  SRR4302547.3562727/94‑1 (MQ=60)

                                                          AAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTG                                     >  SRR4302547.1080456/1‑94 (MQ=60)

                                                          AAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTC                                     <  SRR4302547.4675204/94‑1 (MQ=60)

                                                           AGCCGCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCG                                    <  SRR4302547.6376459/94‑1 (MQ=60)

                                                               GCCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGA                                >  SRR4302547.957158/1‑94 (MQ=60)

                                                                CCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGAC                               <  SRR4302547.2702784/94‑1 (MQ=60)

                                                                CCATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGCTTTACGCAGTAAGTCGGTGAC                               >  SRR4302547.4001118/1‑94 (MQ=60)

                                                                 aacccccccccccaaccccccacGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACA                              <  SRR4302547.967555/71‑1 (MQ=60)

                                                                 CATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACA                              >  SRR4302547.3106070/1‑94 (MQ=60)

                                                                 CATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACA                              >  SRR4302547.3421133/1‑94 (MQ=60)

                                                                 CATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACA                              >  SRR4302547.4006992/1‑94 (MQ=60)

                                                                 CATCATCGCCACCCGTCCAACAAGCGAGCACCCCACCAGACAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACA                              >  SRR4302547.2199219/1‑94 (MQ=60)

                                                                  ATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACAT                             >  SRR4302547.5588518/1‑94 (MQ=60)

                                                                  ATCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACAT                             <  SRR4302547.3847090/94‑1 (MQ=60)

                                                                   TCATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATC                            <  SRR4302547.4221737/94‑1 (MQ=60)

                                                                    CATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCA                           <  SRR4302547.3451711/94‑1 (MQ=60)

                                                                     ATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCAT                          >  SRR4302547.5609156/1‑94 (MQ=60)

                                                                     ATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCAT                          >  SRR4302547.4570426/1‑94 (MQ=60)

                                                                     ATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCAT                          >  SRR4302547.2198972/1‑94 (MQ=60)

                                                                     ATCGCCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCAT                          >  SRR4302547.2393238/1‑94 (MQ=60)

                                                                       CGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCA                        <  SRR4302547.1221206/94‑1 (MQ=60)

                                                                         CCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAA                      >  SRR4302547.3790693/1‑94 (MQ=60)

                                                                         CCACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAA                      <  SRR4302547.6171250/94‑1 (MQ=60)

                                                                          CACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAT                     >  SRR4302547.6003129/1‑94 (MQ=60)

                                                                          CACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAT                     >  SRR4302547.6221953/1‑94 (MQ=60)

                                                                          CACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAT                     >  SRR4302547.6284600/1‑94 (MQ=60)

                                                                           CCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATC                    <  SRR4302547.1222735/94‑1 (MQ=60)

                                                                           ACCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATC                    >  SRR4302547.633349/1‑94 (MQ=60)

                                                                            CCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCC                   >  SRR4302547.4769546/1‑94 (MQ=60)

                                                                             CCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCA                  <  SRR4302547.3760852/94‑1 (MQ=60)

                                                                             CCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCA                  <  SRR4302547.2520740/94‑1 (MQ=60)

                                                                             CCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCA                  >  SRR4302547.2615053/1‑94 (MQ=60)

                                                                              CGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAT                 >  SRR4302547.5667005/1‑94 (MQ=60)

                                                                              CGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCAT                 <  SRR4302547.6314185/94‑1 (MQ=60)

                                                                               GTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATC                >  SRR4302547.3604057/1‑94 (MQ=60)

                                                                               GTCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATC                >  SRR4302547.4952674/1‑94 (MQ=60)

                                                                                TCCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCG               <  SRR4302547.4067849/94‑1 (MQ=60)

                                                                                 CCACCAAGCGAGCACCCTACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGA              <  SRR4302547.4202751/94‑1 (MQ=60)

                                                                                 CCACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAGCCATCGA              >  SRR4302547.4257838/1‑94 (MQ=60)

                                                                                  CACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAC             >  SRR4302547.4528019/1‑94 (MQ=60)

                                                                                  CACCAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGGTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAC             >  SRR4302547.4181699/1‑94 (MQ=60)

                                                                                   ACCAATCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTGTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACG            <  SRR4302547.745375/94‑1 (MQ=60)

                                                                                     CAAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCT          <  SRR4302547.6778656/94‑1 (MQ=60)

                                                                                     CAAGCGACCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCT          <  SRR4302547.2951062/94‑1 (MQ=60)

                                                                                      AAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTA         >  SRR4302547.1603550/1‑94 (MQ=60)

                                                                                      AAGCGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTA         >  SRR4302547.5624806/1‑94 (MQ=60)

                                                                                         CGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATC      <  SRR4302547.6481137/94‑1 (MQ=60)

                                                                                         CGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATC      <  SRR4302547.5255702/94‑1 (MQ=60)

                                                                                         CGAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATC      <  SRR4302547.980269/94‑1 (MQ=60)

                                                                                          GAGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCG     >  SRR4302547.5970497/1‑94 (MQ=60)

                                                                                           AGCACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGC    >  SRR4302547.288826/1‑94 (MQ=60)

                                                                                             CACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCG  >  SRR4302547.1817765/1‑94 (MQ=60)

TGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCG  >  NC_000913/2375266‑2375452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`