Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,563,766	A→G	V380A (GTG→GCG)	b2451 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,563,766	0	A	G	86.0%	665.7 / 93.4	257	V380A (GTG→GCG)	b2451	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (18/18); new base G (103/118); total (121/136)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 273 total aligned reads displayed.

CCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACC  >  NC_000913/2563676‑2563855

 cGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAg                                                                                        <  1:1817507/93‑1 (MQ=255)

   gCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGc                                                                                      <  2:5210821/93‑1 (MQ=255)

    ccaccaccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGCTGACCGTCAGTACCGCGGCGTAACGCACAGGCa                                                                                     >  1:5140074/1‑93 (MQ=255)

    ccaccaccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCa                                                                                     >  2:5700471/1‑93 (MQ=255)

      accaccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGc                                                                                   >  2:2903552/1‑93 (MQ=255)

       ccaccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCg                                                                                  <  1:921299/93‑1 (MQ=255)

        caccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGa                                                                                 <  2:3170190/93‑1 (MQ=255)

         accaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAg                                                                                <  2:1811426/93‑1 (MQ=255)

          ccaGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAgg                                                                               <  1:5850509/93‑1 (MQ=255)

           cagcagGGGATGCGGATTCGTAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGc                                                                              >  2:360621/1‑93 (MQ=255)

           cagcagGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGc                                                                              <  1:1031991/93‑1 (MQ=255)

           cagcagGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGc                                                                              <  2:1129300/93‑1 (MQ=255)

            agcagGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCg                                                                             >  1:2019960/1‑93 (MQ=255)

            agcagGGGATGCGGATTCGGAAAACGCGCGACGAAAGCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCg                                                                             <  2:3008413/93‑1 (MQ=255)

                ggggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGaa                                                                         <  1:4993245/93‑1 (MQ=255)

                ggggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGaa                                                                         <  1:2480714/93‑1 (MQ=255)

                 gggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAg                                                                        <  2:1721223/93‑1 (MQ=255)

                 gggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAg                                                                        <  2:3083009/93‑1 (MQ=255)

                  ggATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAgc                                                                       >  1:3513727/1‑93 (MQ=255)

                   gATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAgcg                                                                      <  2:5564222/93‑1 (MQ=255)

                    aTGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAgcgc                                                                     <  2:4720550/93‑1 (MQ=255)

                      gCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCa                                                                   <  1:4561398/93‑1 (MQ=255)

                      gCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCa                                                                   >  1:3415239/1‑93 (MQ=255)

                      gCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCa                                                                   >  2:870495/1‑93 (MQ=255)

                       cGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAg                                                                  <  2:4900649/93‑1 (MQ=255)

                        ggATTCGGCAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGc                                                                 >  1:6487199/1‑93 (MQ=255)

                        ggATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGc                                                                 <  2:746506/93‑1 (MQ=255)

                          aTTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCAc                                                               <  2:2021418/93‑1 (MQ=255)

                          aTTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCAc                                                               >  1:3443995/1‑93 (MQ=255)

                             cgtaaaaCTGGCGACGAAAGCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTa                                                            <  1:5019693/90‑1 (MQ=255)

                             cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTa                                                            >  1:2576588/1‑93 (MQ=255)

                             cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTa                                                            >  1:303258/1‑93 (MQ=255)

                             cGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTa                                                            <  1:2936562/93‑1 (MQ=255)

                               gAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTAcg                                                          >  2:3245932/1‑93 (MQ=255)

                               gAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTAcg                                                          <  2:3798644/93‑1 (MQ=255)

                                aaaaCGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGGAGCGCCAGCACGTAcgc                                                         >  1:5713722/1‑93 (MQ=255)

                                aaaaCGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTAcgc                                                         >  2:2841712/1‑93 (MQ=255)

                                   aCGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTc                                                      >  2:3431363/1‑93 (MQ=255)

                                   aCGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTc                                                      <  2:6416732/93‑1 (MQ=255)

                                   aCGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTc                                                      <  2:6191362/93‑1 (MQ=255)

                                     gcgcgACGTAATGGACCAACGCGTTGATGCCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAg                                                    <  1:2390864/93‑1 (MQ=255)

                                       ccgacgaAATCGACCAACGCGTTGATGCCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGtt                                                  <  2:4090311/92‑1 (MQ=255)

                                        cgacgaAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGttt                                                 >  1:481250/1‑93 (MQ=255)

                                          acgaAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTg                                               >  1:4894869/1‑93 (MQ=255)

                                            gaAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTggg                                             >  1:5091324/1‑93 (MQ=255)

                                             aaaTCGACCAGCGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGa                                            <  2:3945662/93‑1 (MQ=255)

                                             aaaTCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGa                                            <  2:757190/93‑1 (MQ=255)

                                              aaTCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGAt                                           >  2:3323322/1‑93 (MQ=255)

                                              aaTCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGAt                                           <  1:55766/93‑1 (MQ=255)

                                               aTCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATc                                          >  1:1899437/1‑93 (MQ=255)

                                                tCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCa                                         <  1:3335169/93‑1 (MQ=255)

                                                 cGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCaa                                        <  1:5449875/93‑1 (MQ=255)

                                                    ccAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAt                                     >  1:133441/1‑93 (MQ=255)

                                                     cAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATc                                    <  2:4491924/93‑1 (MQ=255)

                                                     cAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATc                                    <  1:3765081/93‑1 (MQ=255)

                                                      aaCGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc                                   <  1:2295556/93‑1 (MQ=255)

                                                      aaCGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc                                   <  1:4626740/93‑1 (MQ=255)

                                                      aaCGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc                                   >  1:2867147/1‑93 (MQ=255)

                                                      aaCGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATcc                                   >  1:40951/1‑93 (MQ=255)

                                                       aCGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCa                                  <  2:715551/93‑1 (MQ=255)

                                                        cgcgTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAg                                 <  2:1515687/93‑1 (MQ=255)

                                                         gcgTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGc                                <  2:6285581/93‑1 (MQ=255)

                                                          cgTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCt                               <  1:684078/93‑1 (MQ=255)

                                                           gTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTg                              >  2:4962836/1‑93 (MQ=255)

                                                           gTTGATGACCGCCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGGTTTGGGATCAAGATCCAGCTg                              >  1:6012085/1‑93 (MQ=255)

                                                              gatgaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAAt                           <  2:4043705/93‑1 (MQ=255)

                                                               atgaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATc                          >  2:2590577/1‑93 (MQ=255)

                                                               atgaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATc                          <  2:4751523/93‑1 (MQ=255)

                                                               atgaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATc                          >  1:1445471/1‑93 (MQ=255)

                                                                tgaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCa                         <  1:1633047/93‑1 (MQ=255)

                                                                tgaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCa                         >  1:6165861/1‑93 (MQ=255)

                                                                 gaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAg                        <  2:160421/93‑1 (MQ=255)

                                                                 gaCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAg                        <  2:5719807/93‑1 (MQ=255)

                                                                 gaCCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAg                        <  2:6533690/93‑1 (MQ=255)

                                                                     gTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGcc                    <  2:19663/93‑1 (MQ=255)

                                                                     gTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGcc                    <  1:2803117/93‑1 (MQ=255)

                                                                     ctCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCGCGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGcc                    <  1:6263226/92‑1 (MQ=255)

                                                                      tCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCt                   >  1:3139104/1‑93 (MQ=255)

                                                                       cAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTg                  <  2:5423095/93‑1 (MQ=255)

                                                                         gTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt                >  1:2200545/1‑93 (MQ=255)

                                                                         gTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt                >  1:4348560/1‑93 (MQ=255)

                                                                         gTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAACATCCAGCTGAATCAGCGCCTGtt                >  2:5853501/1‑93 (MQ=255)

                                                                          tACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTg               >  1:6560616/1‑93 (MQ=255)

                                                                           aCCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGc              <  2:2294776/93‑1 (MQ=255)

                                                                            ccGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGcc             >  2:2765111/1‑93 (MQ=255)

                                                                             cgcgGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCa            <  1:5341586/93‑1 (MQ=255)

                                                                             cgcgGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCa            <  1:1492693/93‑1 (MQ=255)

                                                                                 gcgTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcc        >  1:3630700/1‑93 (MQ=255)

                                                                                  cgTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGccc       <  1:2706718/93‑1 (MQ=255)

                                                                                   gTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcccc      <  2:5363483/93‑1 (MQ=255)

                                                                                   gTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcccc      >  2:3820972/1‑93 (MQ=255)

                                                                                   gTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcccc      >  2:252355/1‑93 (MQ=255)

                                                                                    tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGccccc     >  1:5418944/1‑93 (MQ=255)

                                                                                      aCGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCccac   <  2:1364334/93‑1 (MQ=255)

                                                                                      aCGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCccac   <  2:5919442/93‑1 (MQ=255)

                                                                                      aCGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCccac   <  1:3981204/93‑1 (MQ=255)

                                                                                       cgcgCAGGCAGCGGGGCGGGAAGCGCCAGCACGTACACGTCAGTTTAGGGATCAAGATCCAGCTGAAACAGCGCCTGTTGCCAGGCCCccacc  <  1:6651397/93‑1 (MQ=255)

                                                                                       cgcgCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCccacc  <  1:6323855/93‑1 (MQ=255)

                                                                                       cgcgCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCccacc  <  1:5635219/93‑1 (MQ=255)

                                                                                       cgcgCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCccacc  <  1:1122869/93‑1 (MQ=255)

CCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACC  >  NC_000913/2563676‑2563855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGAT  >  NC_000913/2563673‑2563859

TGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCG                                                                                               <  SRR4302547.307578/94‑1 (MQ=60)

 GCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGC                                                                                              >  SRR4302547.5899080/1‑94 (MQ=60)

   CCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAG                                                                                            <  SRR4302547.6576316/94‑1 (MQ=60)

    CGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGG                                                                                           >  SRR4302547.6118584/1‑94 (MQ=60)

    CGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGG                                                                                           <  SRR4302547.1853427/94‑1 (MQ=60)

    CGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGG                                                                                           >  SRR4302547.4713427/1‑94 (MQ=60)

     GGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGC                                                                                          >  SRR4302547.6442817/1‑94 (MQ=60)

      GCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGCTGACCGTCAGTACCGCGGCGTAACGCACAGGCA                                                                                         >  SRR4302547.5245962/1‑94 (MQ=60)

      GCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCA                                                                                         <  SRR4302547.2742492/94‑1 (MQ=60)

       CCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAG                                                                                        >  SRR4302547.1077480/1‑94 (MQ=60)

       CCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAG                                                                                        <  SRR4302547.1178313/94‑1 (MQ=60)

       CCACCACCAGAAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACAATCGCGTTGATGACCGTCAGTGCCGCGGCGTAACGCGCAGGCAG                                                                                        <  SRR4302547.6117648/94‑1 (MQ=60)

         ACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCG                                                                                      >  SRR4302547.6389409/1‑94 (MQ=60)

          CCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGA                                                                                     <  SRR4302547.939091/94‑1 (MQ=60)

          CCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGA                                                                                     >  SRR4302547.3511173/1‑94 (MQ=60)

          CCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGA                                                                                     <  SRR4302547.2976879/94‑1 (MQ=60)

            ACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGG                                                                                   <  SRR4302547.4272944/94‑1 (MQ=60)

             CCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGC                                                                                  <  SRR4302547.1861065/94‑1 (MQ=60)

             CCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGC                                                                                  <  SRR4302547.5971534/94‑1 (MQ=60)

              CAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCG                                                                                 <  SRR4302547.1052280/94‑1 (MQ=60)

              CAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCG                                                                                 >  SRR4302547.2060159/1‑94 (MQ=60)

                 CAGGGGGTTCGGATGCGCAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTGCCGCAGCGTAACGCGCAGGCAGCGAGGCGGTA                                                                              <  SRR4302547.5375326/94‑1 (MQ=60)

                  AGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAA                                                                             <  SRR4302547.3589576/94‑1 (MQ=60)

                  AGGGGATCGGGATTCGGAAGACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCAGCGTAACGCGCAGGCAGCGAGGCGGGCA                                                                             >  SRR4302547.2040974/1‑94 (MQ=60)

                   GGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAG                                                                            <  SRR4302547.5096476/94‑1 (MQ=60)

                   GGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAG                                                                            <  SRR4302547.2530856/94‑1 (MQ=60)

                    GGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGC                                                                           >  SRR4302547.3585460/1‑94 (MQ=60)

                     GGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCG                                                                          <  SRR4302547.2274426/94‑1 (MQ=60)

                      GATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGC                                                                         >  SRR4302547.1156671/1‑94 (MQ=60)

                       ATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCC                                                                        <  SRR4302547.5207526/94‑1 (MQ=60)

                        TGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCA                                                                       >  SRR4302547.3484783/1‑94 (MQ=60)

                         GCGGATTCGGAAAACGCGCGACGAAATCGACCAAGGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAG                                                                      <  SRR4302547.5452199/94‑1 (MQ=60)

                         GCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAG                                                                      <  SRR4302547.2012846/94‑1 (MQ=60)

                         GCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAG                                                                      <  SRR4302547.4655767/94‑1 (MQ=60)

                         GCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAG                                                                      >  SRR4302547.6317762/1‑94 (MQ=60)

                          CGGATTCGGCAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGC                                                                     >  SRR4302547.6622255/1‑94 (MQ=60)

                          CGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCCGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGC                                                                     >  SRR4302547.4068670/1‑94 (MQ=60)

                           GGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCA                                                                    >  SRR4302547.1707165/1‑94 (MQ=60)

                           GGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCA                                                                    <  SRR4302547.5592000/94‑1 (MQ=60)

                            GATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCAC                                                                   >  SRR4302547.3514209/1‑94 (MQ=60)

                             ATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACG                                                                  >  SRR4302547.67882/1‑94 (MQ=60)

                             ATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACG                                                                  <  SRR4302547.4888535/94‑1 (MQ=60)

                               TCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGTAGCGAGGCGGGAAGCGCCAGCACGTA                                                                <  SRR4302547.6230311/94‑1 (MQ=60)

                               TCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTA                                                                >  SRR4302547.2628588/1‑94 (MQ=60)

                               TCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTA                                                                >  SRR4302547.308913/1‑94 (MQ=60)

                                cgtaaaactgGCGACGAAAGCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTAC                                                               <  SRR4302547.5123425/84‑1 (MQ=60)

                                CGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTAC                                                               <  SRR4302547.2995076/94‑1 (MQ=60)

                                CGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTAC                                                               >  SRR4302547.6688356/1‑94 (MQ=60)

                                 GGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACG                                                              >  SRR4302547.6455278/1‑94 (MQ=60)

                                  GAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGGAGCGCCAGCACGTACGC                                                             >  SRR4302547.5831733/1‑94 (MQ=60)

                                      ACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCTTCA                                                         <  SRR4302547.3421593/94‑1 (MQ=60)

                                       CGCGCGACGAACTCCACCAACGCGTTGATGACCGTCAGTCCCGCGGCGTAGCGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAG                                                        <  SRR4302547.5170034/94‑1 (MQ=60)

                                        GCGCGACGTAATGGACCAACGCGTTGATGCCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGT                                                       <  SRR4302547.2439290/94‑1 (MQ=60)

                                        GCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGT                                                       <  SRR4302547.1371256/94‑1 (MQ=60)

                                         CGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCCGTT                                                      >  SRR4302547.3509382/1‑94 (MQ=60)

                                         CGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTT                                                      >  SRR4302547.5959273/1‑94 (MQ=60)

                                         CGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTT                                                      >  SRR4302547.3419045/1‑94 (MQ=60)

                                          GCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTT                                                     >  SRR4302547.490080/1‑94 (MQ=60)

                                           CGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGTGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTT                                                    >  SRR4302547.5053287/1‑94 (MQ=60)

                                            GACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTG                                                   >  SRR4302547.4996246/1‑94 (MQ=60)

                                            GACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTG                                                   <  SRR4302547.3200408/94‑1 (MQ=60)

                                            GACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTG                                                   >  SRR4302547.1500322/1‑94 (MQ=60)

                                              CGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGG                                                 <  SRR4302547.4899510/94‑1 (MQ=60)

                                              CGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGG                                                 >  SRR4302547.5196340/1‑94 (MQ=60)

                                               GAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGA                                                >  SRR4302547.5946905/1‑94 (MQ=60)

                                                AAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGGTTTGGGAT                                               >  SRR4302547.427893/1‑94 (MQ=60)

                                                 AATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATC                                              <  SRR4302547.56815/94‑1 (MQ=60)

                                                 AATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATC                                              >  SRR4302547.1937017/1‑94 (MQ=60)

                                                 AATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATC                                              <  SRR4302547.5650076/94‑1 (MQ=60)

                                                  ATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCA                                             <  SRR4302547.3778458/94‑1 (MQ=60)

                                                   TCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAA                                            <  SRR4302547.3837681/94‑1 (MQ=60)

                                                   TCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAA                                            <  SRR4302547.3402632/94‑1 (MQ=60)

                                                    CGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAG                                           <  SRR4302547.5562481/94‑1 (MQ=60)

                                                     GACCAACGCGTTGATGCCCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGA                                          <  SRR4302547.6732250/94‑1 (MQ=60)

                                                     GACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGA                                          >  SRR4302547.5242639/1‑94 (MQ=60)

                                                      GCCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAT                                         <  SRR4302547.2283067/94‑1 (MQ=60)

                                                      ACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAT                                         >  SRR4302547.5177221/1‑94 (MQ=60)

                                                      ACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAT                                         <  SRR4302547.4498507/94‑1 (MQ=60)

                                                      ACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAT                                         <  SRR4302547.3354884/94‑1 (MQ=60)

                                                      ACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAT                                         >  SRR4302547.6728549/1‑94 (MQ=60)

                                                      ACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGAT                                         >  SRR4302547.135857/1‑94 (MQ=60)

                                                       CCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATC                                        <  SRR4302547.146016/94‑1 (MQ=60)

                                                       CCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATC                                        <  SRR4302547.5537406/94‑1 (MQ=60)

                                                        CAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCC                                       >  SRR4302547.41702/1‑94 (MQ=60)

                                                        CAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCC                                       <  SRR4302547.3841876/94‑1 (MQ=60)

                                                        CAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCC                                       >  SRR4302547.1586927/1‑94 (MQ=60)

                                                        CAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCC                                       >  SRR4302547.2924421/1‑94 (MQ=60)

                                                        CAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCC                                       <  SRR4302547.2154219/94‑1 (MQ=60)

                                                         AACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCA                                      <  SRR4302547.4722561/94‑1 (MQ=60)

                                                         AACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCA                                      <  SRR4302547.2342071/94‑1 (MQ=60)

                                                           CGCGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGC                                    <  SRR4302547.3011666/94‑1 (MQ=60)

                                                             CGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTG                                  <  SRR4302547.5330909/94‑1 (MQ=60)

                                                             CGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTG                                  <  SRR4302547.696709/94‑1 (MQ=60)

                                                             CGTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTG                                  <  SRR4302547.2577784/94‑1 (MQ=60)

                                                             CGTTGATGACCGCCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGGTTTGGGATCAAGATCCAGCTG                                  >  SRR4302547.6136864/1‑94 (MQ=60)

                                                              GTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGA                                 >  SRR4302547.2344076/1‑94 (MQ=60)

                                                              GTTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGA                                 >  SRR4302547.6134503/1‑94 (MQ=60)

                                                              GTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGA                                 >  SRR4302547.3660690/1‑94 (MQ=60)

                                                               TTGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAA                                >  SRR4302547.285897/1‑94 (MQ=60)

                                                                TGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAAT                               >  SRR4302547.4164763/1‑94 (MQ=60)

                                                                TGATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAAT                               >  SRR4302547.2782165/1‑94 (MQ=60)

                                                                 GATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATC                              <  SRR4302547.5709802/94‑1 (MQ=60)

                                                                 GATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATC                              >  SRR4302547.1474376/1‑94 (MQ=60)

                                                                 GATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATC                              >  SRR4302547.2430762/1‑94 (MQ=60)

                                                                  GTGACCGTCAGTGCCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCA                             <  SRR4302547.174888/94‑1 (MQ=60)

                                                                  ATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCA                             >  SRR4302547.5421646/1‑94 (MQ=60)

                                                                  ATGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCA                             >  SRR4302547.6293839/1‑94 (MQ=60)

                                                                   TGACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAG                            <  SRR4302547.1665361/94‑1 (MQ=60)

                                                                    GACTGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGC                           <  SRR4302547.1523902/94‑1 (MQ=60)

                                                                    GACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGC                           >  SRR4302547.2522635/1‑94 (MQ=60)

                                                                    GACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGC                           >  SRR4302547.5823586/1‑94 (MQ=60)

                                                                    GACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGC                           <  SRR4302547.687456/94‑1 (MQ=60)

                                                                     ACCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCG                          >  SRR4302547.2882692/1‑94 (MQ=60)

                                                                      CCGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGC                         >  SRR4302547.2581875/1‑94 (MQ=60)

                                                                       CGTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCC                        >  SRR4302547.4998676/1‑94 (MQ=60)

                                                                        GTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCT                       >  SRR4302547.3201465/1‑94 (MQ=60)

                                                                        GTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCT                       <  SRR4302547.2859234/94‑1 (MQ=60)

                                                                        GTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCT                       <  SRR4302547.2751351/94‑1 (MQ=60)

                                                                        GTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCT                       <  SRR4302547.5547902/94‑1 (MQ=60)

                                                                        CTCAGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCGCGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCT                       <  SRR4302547.6393340/94‑1 (MQ=60)

                                                                           AGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTT                    >  SRR4302547.4437923/1‑94 (MQ=60)

                                                                           AGTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTT                    >  SRR4302547.2244757/1‑94 (MQ=60)

                                                                            GTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTG                   >  SRR4302547.5818634/1‑94 (MQ=60)

                                                                            GTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTG                   >  SRR4302547.6697228/1‑94 (MQ=60)

                                                                            GTACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTG                   <  SRR4302547.3991589/94‑1 (MQ=60)

                                                                             TACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGC                  <  SRR4302547.4957273/94‑1 (MQ=60)

                                                                              ACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCC                 <  SRR4302547.2070665/94‑1 (MQ=60)

                                                                              ACCGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCC                 >  SRR4302547.5125862/1‑94 (MQ=60)

                                                                                CGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAG               <  SRR4302547.5451704/94‑1 (MQ=60)

                                                                                CGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAG               >  SRR4302547.3522188/1‑94 (MQ=60)

                                                                                CGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAG               <  SRR4302547.3072885/94‑1 (MQ=60)

                                                                                CGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAG               <  SRR4302547.2961909/94‑1 (MQ=60)

                                                                                CGCGGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAG               <  SRR4302547.5118803/94‑1 (MQ=60)

                                                                                CGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAG               <  SRR4302547.1522475/94‑1 (MQ=60)

                                                                                   GGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCC            >  SRR4302547.3704762/1‑94 (MQ=60)

                                                                                   GGCGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCC            >  SRR4302547.3228274/1‑94 (MQ=60)

                                                                                     CGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCC          >  SRR4302547.4448160/1‑94 (MQ=60)

                                                                                     CGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCC          >  SRR4302547.4917435/1‑94 (MQ=60)

                                                                                     CGTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCC          <  SRR4302547.2761073/94‑1 (MQ=60)

                                                                                      GTAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCC         <  SRR4302547.90935/94‑1 (MQ=60)

                                                                                      GTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCC         >  SRR4302547.5531034/1‑94 (MQ=60)

                                                                                       TAACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCA        <  SRR4302547.6596178/94‑1 (MQ=60)

                                                                                         ACGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACC      <  SRR4302547.4062162/94‑1 (MQ=60)

                                                                                          CGCGCAGGCAGCGGGGCGGGAAGCGCCAGCACGTACACGTCAGTTTAGGGATCAAGATCCAGCTGAAACAGCGCCTGTTGCCAGGCCCCCACCC     <  SRR4302547.6790040/94‑1 (MQ=60)

                                                                                          CGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCA     <  SRR4302547.5751517/94‑1 (MQ=60)

                                                                                          CGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCA     <  SRR4302547.1145515/94‑1 (MQ=60)

                                                                                          CGCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCA     <  SRR4302547.6455348/94‑1 (MQ=60)

                                                                                           GCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAG    <  SRR4302547.6667527/94‑1 (MQ=60)

                                                                                           GCGCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAG    >  SRR4302547.3338832/1‑94 (MQ=60)

                                                                                             GCAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGAT  <  SRR4302547.6081421/94‑1 (MQ=60)

TGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGAT  >  NC_000913/2563673‑2563859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`