Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,787,653 G→A A216A (GCG→GCA)  b2659 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,787,6530GA81.8% 599.7 / 111.6 247A216A (GCG→GCA) b2659hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base A (101/101);  total (127/120)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-02

Only 100 of 275 total aligned reads displayed.

AAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTT  >  NC_000913/2787563‑2787745
                                                                                          |                                                                                            
aaaTTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTgccgcacc                                                                                            <  2:4197219/93‑1 (MQ=255)
 aaTTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTgccgcgccg                                                                                           <  2:3606629/93‑1 (MQ=255)
   ttCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCaccgcc                                                                                         >  2:2958211/1‑93 (MQ=255)
     cGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGa                                                                                       <  1:3211033/93‑1 (MQ=255)
       ctgctgctgCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGt                                                                                     <  1:3007694/93‑1 (MQ=255)
        tgctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTa                                                                                    <  1:24655/93‑1 (MQ=255)
        tgctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTa                                                                                    <  1:101904/93‑1 (MQ=255)
        tgctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTa                                                                                    <  1:5887133/93‑1 (MQ=255)
        tgctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTa                                                                                    >  2:6473793/1‑93 (MQ=255)
        tgctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGACACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTa                                                                                    >  1:951022/1‑93 (MQ=255)
         gctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTaa                                                                                   >  1:4264428/1‑93 (MQ=255)
          ctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTaaa                                                                                  >  2:2681467/1‑93 (MQ=255)
          ctgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTaaa                                                                                  >  1:3819131/1‑93 (MQ=255)
           tgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTaaaa                                                                                 >  2:4406113/1‑93 (MQ=255)
           tgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTaaaa                                                                                 <  1:4969040/93‑1 (MQ=255)
           tgctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTaaaa                                                                                 >  2:4515552/1‑93 (MQ=255)
            gctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTaaaaa                                                                                <  1:2703986/93‑1 (MQ=255)
             ctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAAc                                                                               >  1:4348363/1‑93 (MQ=255)
             ctgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAAc                                                                               <  2:5775457/93‑1 (MQ=255)
              tgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACg                                                                              <  1:5269821/93‑1 (MQ=255)
              tgcATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACg                                                                              >  2:1957289/1‑93 (MQ=255)
                cATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTc                                                                            >  1:2274786/1‑93 (MQ=255)
                cATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTc                                                                            <  1:6085343/93‑1 (MQ=255)
                 aTCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCa                                                                           <  2:4324589/93‑1 (MQ=255)
                 aTCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCa                                                                           >  1:2567965/1‑93 (MQ=255)
                 aTCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCa                                                                           >  2:5352807/1‑93 (MQ=255)
                 aTCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCa                                                                           <  2:3281522/93‑1 (MQ=255)
                  tctcGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAg                                                                          <  1:735361/93‑1 (MQ=255)
                  tctcGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAg                                                                          >  2:1602471/1‑93 (MQ=255)
                  tctcGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAg                                                                          >  2:3169280/1‑93 (MQ=255)
                   ctcGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGc                                                                         >  2:4644761/1‑93 (MQ=255)
                    tcGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCa                                                                        >  2:5732329/1‑93 (MQ=255)
                      gATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCaaa                                                                      <  1:4482190/93‑1 (MQ=255)
                      gATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCaaa                                                                      >  1:260332/1‑93 (MQ=255)
                         gACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGAt                                                                   >  1:3629939/1‑93 (MQ=255)
                          aCTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATg                                                                  >  1:5510124/1‑93 (MQ=255)
                          aCTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATg                                                                  >  2:6015502/1‑93 (MQ=255)
                           cTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGt                                                                 <  2:3356405/93‑1 (MQ=255)
                            tGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGtt                                                                <  2:6162392/93‑1 (MQ=255)
                             gggAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGttt                                                               <  1:5107557/93‑1 (MQ=255)
                             gggAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGttt                                                               >  2:6371601/1‑93 (MQ=255)
                             gggAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGttt                                                               >  1:1135308/1‑93 (MQ=255)
                              ggAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGtttt                                                              <  2:1732525/93‑1 (MQ=255)
                              ggAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGtttt                                                              <  1:4647083/93‑1 (MQ=255)
                                aaCATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTc                                                            <  2:4721658/93‑1 (MQ=255)
                                 aCATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCa                                                           <  1:4518793/93‑1 (MQ=255)
                                  cATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCAt                                                          >  2:2218942/1‑93 (MQ=255)
                                    tctcGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATcc                                                        <  2:2426248/93‑1 (MQ=255)
                                      tcGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCgg                                                      <  2:2630580/93‑1 (MQ=255)
                                       cGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgt                                                     >  2:570704/1‑93 (MQ=255)
                                       cGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgt                                                     <  2:1292865/93‑1 (MQ=255)
                                       cGACAACTATTCCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgt                                                     <  1:5745875/93‑1 (MQ=255)
                                        gACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgtg                                                    <  1:6390034/93‑1 (MQ=255)
                                        gACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgtg                                                    <  2:3787503/93‑1 (MQ=255)
                                         aCAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgtgt                                                   >  2:5053186/1‑93 (MQ=255)
                                         aCAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGgtgt                                                   >  2:4269101/1‑93 (MQ=255)
                                            aCTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCg                                                <  2:3103443/93‑1 (MQ=255)
                                              tATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGAc                                              <  2:5371655/93‑1 (MQ=255)
                                                tttCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGt                                            <  1:1665638/93‑1 (MQ=255)
                                                  tCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCg                                          <  1:5846455/93‑1 (MQ=255)
                                                   ccGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGa                                         >  1:2832134/1‑93 (MQ=255)
                                                     gCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATc                                       <  2:4161044/93‑1 (MQ=255)
                                                      ccACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCa                                      >  2:2525099/1‑93 (MQ=255)
                                                       cACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCaa                                     >  1:3366728/1‑93 (MQ=255)
                                                       cACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCaa                                     >  2:3916215/1‑93 (MQ=255)
                                                        aCCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAAc                                    <  1:4011995/93‑1 (MQ=255)
                                                         cccGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACa                                   >  2:2707238/1‑93 (MQ=255)
                                                          ccGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAg                                  <  2:1417487/93‑1 (MQ=255)
                                                          ccGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAg                                  <  2:2347369/93‑1 (MQ=255)
                                                            gCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAggg                                >  1:714339/1‑93 (MQ=255)
                                                            gCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAggg                                >  2:4965949/1‑93 (MQ=255)
                                                            gCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAggg                                >  1:522933/1‑93 (MQ=255)
                                                             cTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGt                               <  2:4006525/93‑1 (MQ=255)
                                                               ggCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCg                             >  2:56250/1‑93 (MQ=255)
                                                               ggCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCg                             >  2:6272949/1‑93 (MQ=255)
                                                                  gcgTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGccc                          <  1:1409555/93‑1 (MQ=255)
                                                                  gcgTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGccc                          >  2:3037755/1‑93 (MQ=255)
                                                                   cgTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCg                         <  1:6279311/93‑1 (MQ=255)
                                                                   cgTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGGGTTCGACGTCGATAAACAGGGTCGCCCg                         >  2:5088471/1‑93 (MQ=255)
                                                                    gTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCgg                        >  1:4416995/1‑93 (MQ=255)
                                                                    gTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCgg                        >  1:5446316/1‑93 (MQ=255)
                                                                     tCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGt                       >  2:4662383/1‑93 (MQ=255)
                                                                       gCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGa                     <  2:6358528/93‑1 (MQ=255)
                                                                       gCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGa                     <  1:3879589/93‑1 (MQ=255)
                                                                       gCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGa                     >  2:5565830/1‑93 (MQ=255)
                                                                        cccGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGAt                    <  1:2045902/93‑1 (MQ=255)
                                                                        cccGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGAt                    <  1:809466/93‑1 (MQ=255)
                                                                         ccGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATg                   >  1:3154695/1‑93 (MQ=255)
                                                                         ccGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATg                   <  1:5655226/93‑1 (MQ=255)
                                                                          cGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCCTCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATgc                  <  1:3198506/93‑1 (MQ=255)
                                                                          cGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATgc                  >  1:4546997/1‑93 (MQ=255)
                                                                           gATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATgcg                 >  2:2088801/1‑93 (MQ=255)
                                                                            aTGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATgcgc                >  1:5055491/1‑93 (MQ=255)
                                                                             tGCGCTTTTCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCt               >  1:6478108/1‑93 (MQ=255)
                                                                              gcgcTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCta              <  2:702345/93‑1 (MQ=255)
                                                                                gcTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCtata            >  1:1933724/1‑93 (MQ=255)
                                                                                  tttGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATc          <  1:2890808/93‑1 (MQ=255)
                                                                                  tttGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATc          <  1:2959227/93‑1 (MQ=255)
                                                                                     tccgcaccgcCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGAc       <  1:557801/92‑1 (MQ=255)
                                                                                       cgcaccgcCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCa     >  2:365635/1‑93 (MQ=255)
                                                                                          |                                                                                            
AAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTT  >  NC_000913/2787563‑2787745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTC  >  NC_000913/2787560‑2787746
                                                                                             |                                                                                             
CGGAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCA                                                                                               >  SRR4302547.1423876/1‑94 (MQ=60)
CGGAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCA                                                                                               <  SRR4302547.2811199/94‑1 (MQ=60)
CGGAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCA                                                                                               <  SRR4302547.2995742/94‑1 (MQ=60)
 GGAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCAC                                                                                              >  SRR4302547.2198493/1‑94 (MQ=60)
  GAAATTCGCTGCTGCTGCATCTCGATGACTGTGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACC                                                                                             <  SRR4302547.2135470/94‑1 (MQ=60)
  GAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACC                                                                                             <  SRR4302547.1123363/94‑1 (MQ=60)
    AATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGC                                                                                           <  SRR4302547.786146/94‑1 (MQ=60)
    AATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGC                                                                                           <  SRR4302547.727601/94‑1 (MQ=60)
    AATTCGCTGCTGCTGCATCTCGATGACTGGAAACATCTCGACAACTATTGCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTTCCGCACCGC                                                                                           <  SRR4302547.57724/94‑1 (MQ=60)
     ATTCGCTTCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCC                                                                                          <  SRR4302547.5938097/94‑1 (MQ=60)
     ATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTTCCGCACCGCC                                                                                          <  SRR4302547.6519847/94‑1 (MQ=60)
     ATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCC                                                                                          <  SRR4302547.1066536/94‑1 (MQ=60)
      TTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCG                                                                                         <  SRR4302547.5157136/94‑1 (MQ=60)
       TCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGA                                                                                        >  SRR4302547.5979814/1‑94 (MQ=60)
        CGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAG                                                                                       <  SRR4302547.3275094/94‑1 (MQ=60)
        CGCTGCTGCTGCAACTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAG                                                                                       <  SRR4302547.4813879/94‑1 (MQ=60)
         GCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGT                                                                                      >  SRR4302547.601022/1‑94 (MQ=60)
          CTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTA                                                                                     <  SRR4302547.3067509/94‑1 (MQ=60)
          CTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTA                                                                                     >  SRR4302547.3054225/1‑94 (MQ=60)
          CTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTA                                                                                     <  SRR4302547.2953475/94‑1 (MQ=60)
          CTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGACGCGCTTTGCCGCACCGCCGAGTA                                                                                     >  SRR4302547.2660414/1‑94 (MQ=60)
          CTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGACACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTA                                                                                     >  SRR4302547.969489/1‑94 (MQ=60)
           TGCTGCTGCATCTCGATGACTGGGAGCATCTCGACAACTATTTCCGCCAGCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAA                                                                                    >  SRR4302547.2582556/1‑94 (MQ=60)
           TGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAA                                                                                    <  SRR4302547.25113/94‑1 (MQ=60)
           TGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAA                                                                                    <  SRR4302547.103777/94‑1 (MQ=60)
           TGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAA                                                                                    >  SRR4302547.4351754/1‑94 (MQ=60)
           TGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAA                                                                                    <  SRR4302547.6009016/94‑1 (MQ=60)
            TCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAA                                                                                   <  SRR4302547.2047248/94‑1 (MQ=60)
            GCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAA                                                                                   >  SRR4302547.3896952/1‑94 (MQ=60)
              TGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAA                                                                                 <  SRR4302547.2573110/94‑1 (MQ=60)
              TGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAA                                                                                 >  SRR4302547.3787170/1‑94 (MQ=60)
              TGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAA                                                                                 >  SRR4302547.4837729/1‑94 (MQ=60)
              TGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAA                                                                                 <  SRR4302547.5071864/94‑1 (MQ=60)
               GCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAAC                                                                                >  SRR4302547.4437722/1‑94 (MQ=60)
               GCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAACAAC                                                                                >  SRR4302547.4525877/1‑94 (MQ=60)
               GCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAAC                                                                                <  SRR4302547.2758249/94‑1 (MQ=60)
               GCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAAC                                                                                <  SRR4302547.3139628/94‑1 (MQ=60)
                 TGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAACACGT                                                                              >  SRR4302547.3806476/1‑94 (MQ=60)
                 TGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGT                                                                              >  SRR4302547.5949550/1‑94 (MQ=60)
                 TGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGT                                                                              <  SRR4302547.5378402/94‑1 (MQ=60)
                  GCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTC                                                                             >  SRR4302547.2320788/1‑94 (MQ=60)
                   CATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCA                                                                            <  SRR4302547.6211588/94‑1 (MQ=60)
                   CATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCA                                                                            >  SRR4302547.2619830/1‑94 (MQ=60)
                    ATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAG                                                                           >  SRR4302547.6556229/1‑94 (MQ=60)
                     TCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGC                                                                          <  SRR4302547.749109/94‑1 (MQ=60)
                      CTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCA                                                                         >  SRR4302547.5829371/1‑94 (MQ=60)
                      CTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCA                                                                         >  SRR4302547.1406077/1‑94 (MQ=60)
                      CTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCA                                                                         <  SRR4302547.168900/94‑1 (MQ=60)
                        CGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAA                                                                       >  SRR4302547.265195/1‑94 (MQ=60)
                         GATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAG                                                                      <  SRR4302547.4574789/94‑1 (MQ=60)
                         GATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAG                                                                      <  SRR4302547.1856372/94‑1 (MQ=60)
                          ATGACGGGAAACATCTCGACAACTACTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGA                                                                     <  SRR4302547.5246890/94‑1 (MQ=60)
                           TGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGAT                                                                    <  SRR4302547.4610789/94‑1 (MQ=60)
                           TGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGAT                                                                    >  SRR4302547.3703986/1‑94 (MQ=60)
                            GACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATG                                                                   >  SRR4302547.5769263/1‑94 (MQ=60)
                            GACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATG                                                                   >  SRR4302547.5623886/1‑94 (MQ=60)
                             ACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGT                                                                  >  SRR4302547.746305/1‑94 (MQ=60)
                              CTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTT                                                                 <  SRR4302547.6768998/94‑1 (MQ=60)
                               TGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTCAAAACGTCCGCAAACATGTTT                                                                >  SRR4302547.3051383/1‑94 (MQ=60)
                               TGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTT                                                                >  SRR4302547.1158235/1‑94 (MQ=60)
                               TGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTT                                                                >  SRR4302547.5551317/1‑94 (MQ=60)
                               TGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTG                                                                <  SRR4302547.3507224/94‑1 (MQ=60)
                                GGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTT                                                               <  SRR4302547.5212818/94‑1 (MQ=60)
                                GGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTT                                                               <  SRR4302547.360247/94‑1 (MQ=60)
                                GGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTT                                                               >  SRR4302547.5164975/1‑94 (MQ=60)
                                 GGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTT                                                              <  SRR4302547.2812142/94‑1 (MQ=60)
                                 GGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTT                                                              <  SRR4302547.4743345/94‑1 (MQ=60)
                                 GGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTT                                                              >  SRR4302547.4357569/1‑94 (MQ=60)
                                   AACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCA                                                            <  SRR4302547.1593957/94‑1 (MQ=60)
                                    ACCTCTCGACAACTTTTTCCGCCACCCGCTGGCGGGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCAT                                                           <  SRR4302547.2130165/94‑1 (MQ=60)
                                    ACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCAT                                                           <  SRR4302547.4612146/94‑1 (MQ=60)
                                     CATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATC                                                          >  SRR4302547.4592560/1‑94 (MQ=60)
                                     CATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATC                                                          >  SRR4302547.5817074/1‑94 (MQ=60)
                                       TCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCG                                                        <  SRR4302547.4200057/94‑1 (MQ=60)
                                        CTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGG                                                       >  SRR4302547.3237639/1‑94 (MQ=60)
                                          CGACACCTATTTCCGCCGCCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTG                                                     <  SRR4302547.4640262/94‑1 (MQ=60)
                                          CGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTG                                                     >  SRR4302547.2893968/1‑94 (MQ=60)
                                          CGACAACTATTCCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTG                                                     <  SRR4302547.5864630/94‑1 (MQ=60)
                                           GACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGT                                                    <  SRR4302547.2710249/94‑1 (MQ=60)
                                           GACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGT                                                    <  SRR4302547.6523364/94‑1 (MQ=60)
                                            ACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTT                                                   >  SRR4302547.802641/1‑94 (MQ=60)
                                              AACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCG                                                 >  SRR4302547.5110937/1‑94 (MQ=60)
                                               ACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGA                                                >  SRR4302547.2424857/1‑94 (MQ=60)
                                               ACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGA                                                <  SRR4302547.6731409/94‑1 (MQ=60)
                                                CTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGAC                                               >  SRR4302547.5358564/1‑94 (MQ=60)
                                                CTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGAC                                               >  SRR4302547.607332/1‑94 (MQ=60)
                                                CTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGGAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGAC                                               >  SRR4302547.4623310/1‑94 (MQ=60)
                                                   TTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTC                                            >  SRR4302547.2250099/1‑94 (MQ=60)
                                                   TTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTC                                            <  SRR4302547.1698471/94‑1 (MQ=60)
                                                     TCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGA                                          >  SRR4302547.2888764/1‑94 (MQ=60)
                                                     TCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGA                                          <  SRR4302547.5967394/94‑1 (MQ=60)
                                                        GCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCA                                       >  SRR4302547.894015/1‑94 (MQ=60)
                                                        GCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCA                                       >  SRR4302547.5601058/1‑94 (MQ=60)
                                                        CCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCA                                       <  SRR4302547.6065976/94‑1 (MQ=60)
                                                         CCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAA                                      >  SRR4302547.3435110/1‑94 (MQ=60)
                                                          CACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAAC                                     >  SRR4302547.572890/1‑94 (MQ=60)
                                                           CCCCGCGGGCGCGTCGCCCGATGCGCTTTGCCGCACCGACGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACA                                    <  SRR4302547.4485291/94‑1 (MQ=60)
                                                           ACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACA                                    <  SRR4302547.4093547/94‑1 (MQ=60)
                                                            ctagcgggcacgttgccctATGCGCTCTGCCGCTCCGCAGAGTAAAAACGTCAACAAAGATGTTTTTCATCCGGTGTTCAACGTCGATCAACAG                                   <  SRR4302547.6265487/75‑1 (MQ=60)
                                                             CCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGG                                  >  SRR4302547.4333795/1‑94 (MQ=60)
                                                              CGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGG                                 >  SRR4302547.727558/1‑94 (MQ=60)
                                                              CGCTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGG                                 >  SRR4302547.532402/1‑94 (MQ=60)
                                                               GCTGGCGCGTCGCCCGATGCGCTTTTCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGT                                <  SRR4302547.3328288/94‑1 (MQ=60)
                                                                CTGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTC                               <  SRR4302547.5378133/94‑1 (MQ=60)
                                                                 TGGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCG                              >  SRR4302547.2511440/1‑94 (MQ=60)
                                                                  GGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGC                             <  SRR4302547.3244541/94‑1 (MQ=60)
                                                                  GGCGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGC                             <  SRR4302547.6422459/94‑1 (MQ=60)
                                                                    CGCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGATCGACGTCGATCAACAGGGTCGCCC                           >  SRR4302547.1430171/1‑94 (MQ=60)
                                                                     GCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCG                          <  SRR4302547.2945790/94‑1 (MQ=60)
                                                                     GCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCG                          <  SRR4302547.1437748/94‑1 (MQ=60)
                                                                     GCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCG                          >  SRR4302547.6250264/1‑94 (MQ=60)
                                                                     GCGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCG                          >  SRR4302547.4027822/1‑94 (MQ=60)
                                                                      CGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGG                         <  SRR4302547.6409774/94‑1 (MQ=60)
                                                                      CGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGG                         >  SRR4302547.4508139/1‑94 (MQ=60)
                                                                      CGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGG                         >  SRR4302547.4366602/1‑94 (MQ=60)
                                                                      CGTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGG                         >  SRR4302547.5558847/1‑94 (MQ=60)
                                                                       GTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGT                        <  SRR4302547.1759511/94‑1 (MQ=60)
                                                                       GTCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGT                        <  SRR4302547.2866636/94‑1 (MQ=60)
                                                                        GCGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTG                       <  SRR4302547.4066581/94‑1 (MQ=60)
                                                                         CGCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGCGA                      >  SRR4302547.326335/1‑94 (MQ=60)
                                                                          GCCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGAT                     <  SRR4302547.3958529/94‑1 (MQ=60)
                                                                           CCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATG                    <  SRR4302547.263707/94‑1 (MQ=60)
                                                                           CCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATG                    <  SRR4302547.2086708/94‑1 (MQ=60)
                                                                           CCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATG                    <  SRR4302547.5532996/94‑1 (MQ=60)
                                                                           CCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATG                    >  SRR4302547.3217422/1‑94 (MQ=60)
                                                                           CCCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATA                    <  SRR4302547.824803/94‑1 (MQ=60)
                                                                            CCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGC                   <  SRR4302547.5771977/94‑1 (MQ=60)
                                                                            CCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGC                   >  SRR4302547.4510303/1‑94 (MQ=60)
                                                                            CCGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGC                   >  SRR4302547.4641026/1‑94 (MQ=60)
                                                                             CGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCCTCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCG                  <  SRR4302547.3262267/94‑1 (MQ=60)
                                                                             CGATGCGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCG                  >  SRR4302547.6361842/1‑94 (MQ=60)
                                                                              GATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGC                 >  SRR4302547.5159843/1‑94 (MQ=60)
                                                                               ATGCGCTTTTCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCT                >  SRR4302547.6612991/1‑94 (MQ=60)
                                                                                  CGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATA             >  SRR4302547.1972070/1‑94 (MQ=60)
                                                                                  CGCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATA             >  SRR4302547.3173895/1‑94 (MQ=60)
                                                                                   GCTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATAT            <  SRR4302547.3391492/94‑1 (MQ=60)
                                                                                    CTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATC           <  SRR4302547.3911343/94‑1 (MQ=60)
                                                                                    CTTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATC           >  SRR4302547.4736014/1‑94 (MQ=60)
                                                                                     TTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCG          <  SRR4302547.566709/94‑1 (MQ=60)
                                                                                     TTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCG          <  SRR4302547.2948467/94‑1 (MQ=60)
                                                                                     TTTGCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCG          <  SRR4302547.3018125/94‑1 (MQ=60)
                                                                                        TCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACC       <  SRR4302547.567854/94‑1 (MQ=60)
                                                                                        GCCGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACC       <  SRR4302547.5619551/94‑1 (MQ=60)
                                                                                          CGCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAG     >  SRR4302547.2448829/1‑94 (MQ=60)
                                                                                           GCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGT    <  SRR4302547.5467178/94‑1 (MQ=60)
                                                                                           GCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGT    >  SRR4302547.3893919/1‑94 (MQ=60)
                                                                                           GCACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGT    <  SRR4302547.176335/94‑1 (MQ=60)
                                                                                            CACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTT   >  SRR4302547.769643/1‑94 (MQ=60)
                                                                                            CACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGCTGATGCGCTATATCGACCAGGT   >  SRR4302547.5491134/1‑94 (MQ=60)
                                                                                             GCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTC  <  SRR4302547.5163163/94‑1 (MQ=60)
                                                                                             ACCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTC  >  SRR4302547.1646914/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGAAATTCGCTGCTGCTGCATCTCGATGACTGGGAACATCTCGACAACTATTTCCGCCACCCGCTGGCGCGTCGCCCGATGCGCTTTGCCGCGCCGCCGAGTAAAAACGTCAGCAAAGATGTTTTTCATCCGGTGTTCGACGTCGATCAACAGGGTCGCCCGGTGATGCGCTATATCGACCAGTTC  >  NC_000913/2787560‑2787746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑