Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,816,853 T→C intergenic (‑186/+130) serV ← / ← csrA tRNA‑Ser/CsrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,816,8530TC90.8% 817.3 / 54.8 272intergenic (‑186/+130)serV/csrAtRNA‑Ser/CsrA
Reads supporting (aligned to +/- strand):  ref base T (14/11);  new base C (129/118);  total (143/129)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 273 total aligned reads displayed.

TTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT  >  NC_000913/2816763‑2816943
                                                                                          |                                                                                          
ttttCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCt                                                                                          <  1:2950489/93‑1 (MQ=255)
ttttCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCt                                                                                          <  1:2907706/93‑1 (MQ=255)
   tacTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGAc                                                                                       >  1:6551847/3‑93 (MQ=255)
     cTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAg                                                                                     >  1:5488510/1‑93 (MQ=255)
      tACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGa                                                                                    >  2:1765924/1‑93 (MQ=255)
      tACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGa                                                                                    <  2:1979615/93‑1 (MQ=255)
       acacTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAt                                                                                   >  2:4439528/1‑93 (MQ=255)
       acacTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAt                                                                                   >  1:341666/1‑93 (MQ=255)
        cacTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAta                                                                                  >  1:958406/1‑93 (MQ=255)
           ttttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAtagta                                                                               <  2:3318220/93‑1 (MQ=255)
            tttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGAtagtag                                                                              >  2:6212308/1‑93 (MQ=255)
            tttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAtagtag                                                                              >  1:6112059/1‑93 (MQ=255)
             ttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATagtagt                                                                             <  1:3170472/93‑1 (MQ=255)
             ttATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATagtagt                                                                             <  2:2413899/93‑1 (MQ=255)
               aTCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTaa                                                                           >  2:1153324/1‑93 (MQ=255)
                tCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAt                                                                          <  1:5813148/93‑1 (MQ=255)
                tCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAt                                                                          >  1:1444485/1‑93 (MQ=255)
                tCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAt                                                                          >  2:5796654/1‑93 (MQ=255)
                 cGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATg                                                                         <  2:2078665/93‑1 (MQ=255)
                 cGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATg                                                                         <  2:6355499/93‑1 (MQ=255)
                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        >  2:4798572/1‑93 (MQ=255)
                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        >  2:6062856/1‑93 (MQ=255)
                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        <  1:4471640/93‑1 (MQ=255)
                  gTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGc                                                                        >  1:4244287/1‑93 (MQ=255)
                   tttGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCa                                                                       >  1:4960893/1‑93 (MQ=255)
                   tttGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCa                                                                       >  1:2110814/1‑93 (MQ=255)
                      gCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCaaaa                                                                    >  1:3241934/1‑93 (MQ=255)
                       cacaCTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaa                                                                   >  2:1017164/1‑93 (MQ=255)
                        acacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaa                                                                  >  2:474705/1‑93 (MQ=255)
                         cacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaaa                                                                 <  1:2505290/93‑1 (MQ=255)
                         cacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaaa                                                                 >  2:2661917/1‑93 (MQ=255)
                         cacTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCaaaaaaa                                                                 <  2:2988911/93‑1 (MQ=255)
                           cTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATg                                                               >  2:5366053/1‑93 (MQ=255)
                             tCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGga                                                             >  2:2674128/1‑93 (MQ=255)
                             tCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGga                                                             <  2:5148525/93‑1 (MQ=255)
                              cACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgag                                                            >  2:6403406/1‑93 (MQ=255)
                              cACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgag                                                            <  1:1772002/93‑1 (MQ=255)
                              cACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgag                                                            <  2:5689327/93‑1 (MQ=255)
                               aCGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgaga                                                           >  2:6236671/1‑93 (MQ=255)
                               aCGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGgaga                                                           <  1:523252/93‑1 (MQ=255)
                                cGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGAc                                                          <  2:4206048/93‑1 (MQ=255)
                                  ttCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACtt                                                        >  2:4946629/1‑93 (MQ=255)
                                  ttCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACtt                                                        <  2:3243648/93‑1 (MQ=255)
                                  ttCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACtt                                                        >  2:1483332/1‑93 (MQ=255)
                                    cAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTaa                                                      <  2:36126/93‑1 (MQ=255)
                                      aTTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGt                                                    <  1:3372492/93‑1 (MQ=255)
                                      aTTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGt                                                    >  1:1863397/1‑93 (MQ=255)
                                       ttAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGtt                                                   <  1:4849780/93‑1 (MQ=255)
                                        tAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtg                                                  >  1:197148/1‑93 (MQ=255)
                                          gTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaa                                                <  2:4075670/93‑1 (MQ=255)
                                          gTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaa                                                >  1:675496/1‑93 (MQ=255)
                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               <  2:1304566/93‑1 (MQ=255)
                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               >  1:4562870/1‑93 (MQ=255)
                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               <  2:1662041/93‑1 (MQ=255)
                                           tCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaat                                               <  1:557999/93‑1 (MQ=255)
                                            cTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaatg                                              <  2:2299773/93‑1 (MQ=255)
                                             tGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaatga                                             <  1:6209365/93‑1 (MQ=255)
                                              gTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTtgaatgaa                                            >  1:1140469/1‑93 (MQ=255)
                                               tAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAAc                                           >  1:2738755/1‑93 (MQ=255)
                                                   caCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGa                                       <  2:5301700/92‑1 (MQ=255)
                                                   aaCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGa                                       <  2:4329306/93‑1 (MQ=255)
                                                   aaCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGa                                       >  2:5526609/1‑93 (MQ=255)
                                                    aCGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAg                                      >  1:4263509/1‑93 (MQ=255)
                                                       tCAAAAAGAGTGTTTTATCGACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTaa                                   <  2:6131005/93‑1 (MQ=255)
                                                       tCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTaa                                   <  1:764692/93‑1 (MQ=255)
                                                        cAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTaaa                                  <  1:1267769/93‑1 (MQ=255)
                                                         aaaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAg                                 <  2:4182980/93‑1 (MQ=255)
                                                          aaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGc                                >  2:3523521/1‑93 (MQ=255)
                                                          aaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGc                                >  2:3800605/1‑93 (MQ=255)
                                                          aaaaGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGc                                <  1:2851552/93‑1 (MQ=255)
                                                           aaaGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCg                               >  2:3834362/1‑93 (MQ=255)
                                                             agagTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGaa                             >  2:786019/1‑93 (MQ=255)
                                                               agTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGaaaa                           <  2:2273247/93‑1 (MQ=255)
                                                                gtgtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAg                          <  1:1606235/93‑1 (MQ=255)
                                                                 tgtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGa                         >  2:2798837/1‑93 (MQ=255)
                                                                 tgtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGa                         <  1:1592909/93‑1 (MQ=255)
                                                                  gtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAc                        >  1:5066111/1‑93 (MQ=255)
                                                                  gtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAc                        <  1:4191276/93‑1 (MQ=255)
                                                                  gtTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAc                        <  2:143251/93‑1 (MQ=255)
                                                                    tttATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACta                      >  2:1205860/1‑93 (MQ=255)
                                                                    tttATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACta                      <  1:2926062/93‑1 (MQ=255)
                                                                    tttATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACta                      <  2:6615210/93‑1 (MQ=255)
                                                                      tATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACtata                    <  2:4377062/93‑1 (MQ=255)
                                                                      tATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACtata                    >  1:3839333/1‑93 (MQ=255)
                                                                      tATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACtata                    >  2:219061/1‑93 (MQ=255)
                                                                       aTCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATag                   >  2:5071627/1‑93 (MQ=255)
                                                                       aTCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATag                   <  2:6268412/93‑1 (MQ=255)
                                                                        tCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATaga                  <  1:3708237/93‑1 (MQ=255)
                                                                        tCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATaga                  <  1:3465921/93‑1 (MQ=255)
                                                                          aaCAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGt                >  2:5978790/1‑93 (MQ=255)
                                                                            cAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGa              >  2:4600954/1‑93 (MQ=255)
                                                                              gaagaaTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAg            >  1:6313490/1‑93 (MQ=255)
                                                                               aagaaTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAgg           <  1:5349824/93‑1 (MQ=255)
                                                                                  aaTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGgaga        <  1:810758/93‑1 (MQ=255)
                                                                                   aTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGaa       >  1:6037897/1‑93 (MQ=255)
                                                                                   aTGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGaa       >  1:6411049/1‑93 (MQ=255)
                                                                                    tGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGaaa      >  2:255907/1‑93 (MQ=255)
                                                                                      gAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAAtt    >  1:2283434/1‑93 (MQ=255)
                                                                                      gAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAAtt    >  2:3867402/1‑93 (MQ=255)
                                                                                       aGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAAttt   >  1:3974846/1‑93 (MQ=255)
                                                                                          |                                                                                          
TTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT  >  NC_000913/2816763‑2816943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTTGAG  >  NC_000913/2816761‑2816946
                                                                                            |                                                                                             
ATTTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCC                                                                                              <  SRR4302547.4390517/94‑1 (MQ=60)
ATTTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCC                                                                                              <  SRR4302547.3747899/94‑1 (MQ=60)
 TTTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCT                                                                                             >  SRR4302547.155978/1‑94 (MQ=60)
 TTTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCT                                                                                             <  SRR4302547.4079879/94‑1 (MQ=60)
  TTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTG                                                                                            <  SRR4302547.2965692/94‑1 (MQ=60)
  TTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTG                                                                                            <  SRR4302547.3009238/94‑1 (MQ=60)
    TTACTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGAC                                                                                          >  SRR4302547.6688200/1‑94 (MQ=60)
     TCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACA                                                                                         <  SRR4302547.2162259/94‑1 (MQ=60)
     TCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACA                                                                                         <  SRR4302547.2795999/94‑1 (MQ=60)
     TCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACA                                                                                         >  SRR4302547.317944/1‑94 (MQ=60)
      CCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAG                                                                                        >  SRR4302547.4035494/1‑94 (MQ=60)
      CCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAG                                                                                        >  SRR4302547.5601850/1‑94 (MQ=60)
       CTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGA                                                                                       >  SRR4302547.4218971/1‑94 (MQ=60)
        TACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAT                                                                                      >  SRR4302547.348009/1‑94 (MQ=60)
        TACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGAT                                                                                      >  SRR4302547.6452482/1‑94 (MQ=60)
         ACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATA                                                                                     >  SRR4302547.3687843/1‑94 (MQ=60)
         ACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATA                                                                                     >  SRR4302547.977024/1‑94 (MQ=60)
          CACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAG                                                                                    >  SRR4302547.128192/1‑94 (MQ=60)
           ACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGT                                                                                   <  SRR4302547.904179/94‑1 (MQ=60)
           ACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGT                                                                                   >  SRR4302547.4469776/1‑94 (MQ=60)
             TTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAG                                                                                 <  SRR4302547.5555498/94‑1 (MQ=60)
             TTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAG                                                                                 >  SRR4302547.6238848/1‑94 (MQ=60)
             TTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAG                                                                                 <  SRR4302547.1436312/94‑1 (MQ=60)
              TTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGT                                                                                <  SRR4302547.5830745/94‑1 (MQ=60)
               TTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTA                                                                               <  SRR4302547.3233564/94‑1 (MQ=60)
               TTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTA                                                                               <  SRR4302547.2979875/94‑1 (MQ=60)
                 ATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAT                                                                             >  SRR4302547.1473373/1‑94 (MQ=60)
                 ATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAAT                                                                             <  SRR4302547.70692/94‑1 (MQ=60)
                  TCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATG                                                                            <  SRR4302547.5933427/94‑1 (MQ=60)
                  TCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATG                                                                            >  SRR4302547.982585/1‑94 (MQ=60)
                   CGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGC                                                                           >  SRR4302547.1641001/1‑94 (MQ=60)
                   CGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGC                                                                           >  SRR4302547.4331097/1‑94 (MQ=60)
                   CGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGC                                                                           >  SRR4302547.4601056/1‑94 (MQ=60)
                    GTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCA                                                                          <  SRR4302547.4564032/94‑1 (MQ=60)
                    GTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCA                                                                          <  SRR4302547.1757558/94‑1 (MQ=60)
                    GTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCA                                                                          >  SRR4302547.738398/1‑94 (MQ=60)
                    GTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCA                                                                          >  SRR4302547.2152869/1‑94 (MQ=60)
                    GTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCA                                                                          >  SRR4302547.5063551/1‑94 (MQ=60)
                       TGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAA                                                                       >  SRR4302547.3306638/1‑94 (MQ=60)
                       TGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAA                                                                       >  SRR4302547.6748106/1‑94 (MQ=60)
                         CACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAA                                                                     >  SRR4302547.3156254/1‑94 (MQ=60)
                           CACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAAT                                                                   <  SRR4302547.2555925/94‑1 (MQ=60)
                           CACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAAT                                                                   >  SRR4302547.5344961/1‑94 (MQ=60)
                            ACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATG                                                                  <  SRR4302547.5270700/94‑1 (MQ=60)
                            ACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATG                                                                  >  SRR4302547.3510254/1‑94 (MQ=60)
                             CTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGCGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGG                                                                 >  SRR4302547.6166725/1‑94 (MQ=60)
                               TCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAG                                                               <  SRR4302547.6359401/94‑1 (MQ=60)
                               TCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAG                                                               >  SRR4302547.3648750/1‑94 (MQ=60)
                                CACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGA                                                              <  SRR4302547.1807134/94‑1 (MQ=60)
                                 ACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGAC                                                             <  SRR4302547.4665853/94‑1 (MQ=60)
                                 ACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGAC                                                             <  SRR4302547.532725/94‑1 (MQ=60)
                                  CGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACT                                                            >  SRR4302547.4159372/1‑94 (MQ=60)
                                   GTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTT                                                           <  SRR4302547.2214253/94‑1 (MQ=60)
                                       AATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGT                                                       >  SRR4302547.386763/1‑94 (MQ=60)
                                       AATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGT                                                       >  SRR4302547.3275519/1‑94 (MQ=60)
                                       AATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGT                                                       >  SRR4302547.5926044/1‑94 (MQ=60)
                                       AATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGT                                                       >  SRR4302547.1900189/1‑94 (MQ=60)
                                       AATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGT                                                       >  SRR4302547.2695585/1‑94 (MQ=60)
                                        ATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTT                                                      <  SRR4302547.3440992/94‑1 (MQ=60)
                                        ATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTT                                                      <  SRR4302547.3479187/94‑1 (MQ=60)
                                         TTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTG                                                     <  SRR4302547.4950283/94‑1 (MQ=60)
                                         TTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTG                                                     >  SRR4302547.200832/1‑94 (MQ=60)
                                          TAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGA                                                    <  SRR4302547.6292084/94‑1 (MQ=60)
                                           AGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAA                                                   >  SRR4302547.687931/1‑94 (MQ=60)
                                            GTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAAT                                                  >  SRR4302547.4657270/1‑94 (MQ=60)
                                             TCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATG                                                 <  SRR4302547.568053/94‑1 (MQ=60)
                                             TCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATG                                                 >  SRR4302547.2778542/1‑94 (MQ=60)
                                              CTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGA                                                >  SRR4302547.2928160/1‑94 (MQ=60)
                                               TGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAA                                               >  SRR4302547.1163499/1‑94 (MQ=60)
                                               TGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAA                                               >  SRR4302547.2941077/1‑94 (MQ=60)
                                               TGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAA                                               >  SRR4302547.6287052/1‑94 (MQ=60)
                                               TGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAA                                               <  SRR4302547.6338304/94‑1 (MQ=60)
                                                GTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAAC                                              >  SRR4302547.2793704/1‑94 (MQ=60)
                                                GTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAAC                                              >  SRR4302547.781079/1‑94 (MQ=60)
                                                GTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAAC                                              <  SRR4302547.2094200/94‑1 (MQ=60)
                                                  TAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGG                                            >  SRR4302547.5668254/1‑94 (MQ=60)
                                                  AAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGG                                            >  SRR4302547.1917872/1‑94 (MQ=60)
                                                  AAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGG                                            >  SRR4302547.6130350/1‑94 (MQ=60)
                                                  AAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGG                                            <  SRR4302547.1635498/94‑1 (MQ=60)
                                                  AAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGG                                            >  SRR4302547.5360196/1‑94 (MQ=60)
                                                   AAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGG                                           >  SRR4302547.6788644/1‑94 (MQ=60)
                                                   AAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGG                                           <  SRR4302547.433519/94‑1 (MQ=60)
                                                    AAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGA                                          <  SRR4302547.995909/94‑1 (MQ=60)
                                                     AACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAG                                         >  SRR4302547.4350789/1‑94 (MQ=60)
                                                        GTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAA                                      <  SRR4302547.5330284/94‑1 (MQ=60)
                                                         TCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAA                                     <  SRR4302547.779102/94‑1 (MQ=60)
                                                          CAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAG                                    <  SRR4302547.3859303/94‑1 (MQ=60)
                                                          CAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAG                                    <  SRR4302547.1293302/94‑1 (MQ=60)
                                                          CAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAG                                    >  SRR4302547.659568/1‑94 (MQ=60)
                                                          CAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAG                                    <  SRR4302547.4310947/94‑1 (MQ=60)
                                                          CAAAAAGAGAGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAG                                    >  SRR4302547.5603978/1‑94 (MQ=60)
                                                           AAAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGC                                   <  SRR4302547.2379097/94‑1 (MQ=60)
                                                            AAAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCG                                  <  SRR4302547.2908517/94‑1 (MQ=60)
                                                             AAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGA                                 <  SRR4302547.3255184/94‑1 (MQ=60)
                                                             AAAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGA                                 <  SRR4302547.4307189/94‑1 (MQ=60)
                                                              AAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAA                                <  SRR4302547.1472265/94‑1 (MQ=60)
                                                              AAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAA                                >  SRR4302547.1514845/1‑94 (MQ=60)
                                                              AAGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAA                                >  SRR4302547.1832633/1‑94 (MQ=60)
                                                               AGAGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAA                               >  SRR4302547.5055340/1‑94 (MQ=60)
                                                                 AGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAG                             <  SRR4302547.5091215/94‑1 (MQ=60)
                                                                 AGTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAG                             <  SRR4302547.5091216/94‑1 (MQ=60)
                                                                  GTGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGA                            <  SRR4302547.1638076/94‑1 (MQ=60)
                                                                   TGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAC                           <  SRR4302547.1624535/94‑1 (MQ=60)
                                                                   TGTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGAC                           >  SRR4302547.5170645/1‑94 (MQ=60)
                                                                    GTTTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACT                          <  SRR4302547.4276756/94‑1 (MQ=60)
                                                                      TTTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTAT                        <  SRR4302547.2984425/94‑1 (MQ=60)
                                                                       TTATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATA                       >  SRR4302547.3917531/1‑94 (MQ=60)
                                                                        TATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAG                      <  SRR4302547.2984065/94‑1 (MQ=60)
                                                                        TATCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAG                      >  SRR4302547.889251/1‑94 (MQ=60)
                                                                          TCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAG                    <  SRR4302547.3783936/94‑1 (MQ=60)
                                                                          TCAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAG                    <  SRR4302547.3536607/94‑1 (MQ=60)
                                                                           CAACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGT                   >  SRR4302547.3004221/1‑94 (MQ=60)
                                                                            AACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTG                  >  SRR4302547.4610003/1‑94 (MQ=60)
                                                                             ACAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGA                 <  SRR4302547.1825887/94‑1 (MQ=60)
                                                                              CAGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAA                <  SRR4302547.334715/94‑1 (MQ=60)
                                                                               AGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGTGAGTAAAGCGAAAAGACTATAGAGTGAAG               <  SRR4302547.3493574/94‑1 (MQ=60)
                                                                               AGAAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAG               >  SRR4302547.6444774/1‑94 (MQ=60)
                                                                                GAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGG              <  SRR4302547.2342979/94‑1 (MQ=60)
                                                                                 AAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGA             <  SRR4302547.5460138/94‑1 (MQ=60)
                                                                                 AAGAATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGA             >  SRR4302547.3371961/1‑94 (MQ=60)
                                                                                    AATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          >  SRR4302547.4845817/1‑94 (MQ=60)
                                                                                    AATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          <  SRR4302547.826121/94‑1 (MQ=60)
                                                                                    AATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          >  SRR4302547.6163185/1‑94 (MQ=60)
                                                                                    AATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          >  SRR4302547.6544751/1‑94 (MQ=60)
                                                                                    AATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          <  SRR4302547.639373/94‑1 (MQ=60)
                                                                                    AATGGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          >  SRR4302547.6052108/1‑94 (MQ=60)
                                                                                    AATGGAGGCATGACAGATAGTATTAATGCTAATAAATGGAGTCTCAAGTTGAAGGAAGGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAA          <  SRR4302547.6665840/94‑1 (MQ=53)
                                                                                       GGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATT       >  SRR4302547.2329656/1‑94 (MQ=60)
                                                                                       GGAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATT       >  SRR4302547.4406661/1‑94 (MQ=60)
                                                                                        GAGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTT      >  SRR4302547.4055678/1‑94 (MQ=60)
                                                                                         AGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT     >  SRR4302547.1981197/1‑94 (MQ=60)
                                                                                         AGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT     >  SRR4302547.5735566/1‑94 (MQ=60)
                                                                                         AGGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTT     >  SRR4302547.3842598/1‑94 (MQ=60)
                                                                                          GGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTTG    <  SRR4302547.3131680/94‑1 (MQ=60)
                                                                                          GGCCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTTG    <  SRR4302547.6147625/94‑1 (MQ=60)
                                                                                            CCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTTGAG  <  SRR4302547.5678417/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATTTTTCCTACACTTTTATCGTTTGCACACTTCACGTTCAATTAGTCTGTAAAAACGTCAAAAAGAGTGTTTTATCAACAGAAGAATGGAGGTCTGACAGATAGTAGTAATGCAAAAAAATGGAGACTTAAGTTGAATGAACGGGAGTAAAGCGAAAAGACTATAGAGTGAAGGAGAAATTTTGAG  >  NC_000913/2816761‑2816946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑