Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,585	+G	coding (953/2286 nt)	glcF ←	glycolate oxidase iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,582	1	.	G	99.0%	750.2 / ‑0.9	208	coding (956/2286 nt)	glcF	glycolate oxidase iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (117/89); total (118/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 240 total aligned reads displayed.

AGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG  >  NC_000913/3123490‑3123669

 gTTCGGCGTCCATGCGCCCGGGGTTAACCCCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                         <  1:3422342/93‑1 (MQ=255)

 gTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                         <  2:4110236/93‑1 (MQ=255)

 gTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                         >  2:3109791/1‑93 (MQ=255)

 gTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                         <  2:1489340/93‑1 (MQ=255)

 gTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                         <  2:1272405/93‑1 (MQ=255)

 gTTCCGCGTACAGGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                         <  2:2346592/93‑1 (MQ=255)

  ttCCGCGTACATGCGACCTGGGTAAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg                                                                                        <  1:713737/93‑1 (MQ=255)

  ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg                                                                                        <  2:5084238/93‑1 (MQ=255)

  ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg                                                                                        <  1:3210323/93‑1 (MQ=255)

   tCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggg                                                                                       <  1:4018155/93‑1 (MQ=255)

    ccGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gggg                                                                                      <  2:5983208/93‑2 (MQ=255)

     cgcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggggc                                                                                     <  1:1001259/93‑3 (MQ=255)

         tACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAg                                                                                 >  1:1023002/1‑93 (MQ=255)

         tACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAg                                                                                 >  1:1447807/1‑93 (MQ=255)

           cATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGcc                                                                               >  2:5903485/1‑93 (MQ=255)

            aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGcca                                                                              <  1:5351467/93‑1 (MQ=255)

             tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGccac                                                                             <  2:1547718/93‑1 (MQ=255)

                gACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAt                                                                          <  1:5737813/93‑1 (MQ=255)

                  ccGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAtct                                                                        <  2:3413628/93‑1 (MQ=255)

                   cGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAtctc                                                                       >  1:2161049/1‑93 (MQ=255)

                    ggggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTcc                                                                      >  2:4524734/1‑93 (MQ=255)

                     gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCg                                                                     >  1:941319/1‑93 (MQ=255)

                     gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTACg                                                                     >  1:2940488/1‑93 (MQ=255)

                     gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAACCCCg                                                                     >  1:2016314/1‑93 (MQ=255)

                     cggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCg                                                                     >  2:4826400/2‑93 (MQ=255)

                       gTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGc                                                                   >  1:2453426/1‑93 (MQ=255)

                         tAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCAc                                                                 >  2:5813899/1‑93 (MQ=255)

                          aaaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACt                                                                <  2:2590219/93‑1 (MQ=255)

                           aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTa                                                               >  1:4320132/1‑93 (MQ=255)

                           aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTa                                                               >  1:2197184/1‑93 (MQ=255)

                           aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTa                                                               >  1:394570/1‑93 (MQ=255)

                            acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGCCAAAGCCACCATCTCCGGCACTaa                                                              <  1:1203767/93‑1 (MQ=255)

                             cacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTaaa                                                             <  1:4121427/93‑1 (MQ=255)

                             cacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTaaa                                                             >  1:6079320/1‑93 (MQ=255)

                               cgccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGc                                                           >  1:4082600/1‑93 (MQ=255)

                               cgccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGc                                                           <  2:5741470/93‑1 (MQ=255)

                                 ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCgg                                                         <  1:5217437/93‑1 (MQ=255)

                                  cgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCggg                                                        >  2:6569938/1‑93 (MQ=255)

                                   gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGt                                                       >  1:2727399/1‑93 (MQ=255)

                                     aaGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCg                                                     >  2:5494945/1‑93 (MQ=255)

                                      aGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAGGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGc                                                    >  2:4667166/1‑93 (MQ=255)

                                      aGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGc                                                    >  1:334177/1‑93 (MQ=255)

                                        ggTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAt                                                  >  2:3349392/1‑93 (MQ=255)

                                         gTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATg                                                 >  2:1442388/1‑93 (MQ=255)

                                          tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGa                                                >  1:5281860/1‑93 (MQ=255)

                                          tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGa                                                <  1:483300/93‑1 (MQ=255)

                                           cGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAc                                               <  2:5473839/93‑1 (MQ=255)

                                             aGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg                                             >  2:6361456/1‑93 (MQ=255)

                                                tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          >  2:6174519/1‑93 (MQ=255)

                                                tgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCa                                          <  2:1360254/93‑1 (MQ=255)

                                                 gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                         <  1:6570380/93‑1 (MQ=255)

                                                 gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                         <  1:3252934/93‑1 (MQ=255)

                                                 gctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAACGGGTCGCATTACCGCCAg                                         >  1:5683753/1‑93 (MQ=255)

                                                  ctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGc                                        >  1:5490745/1‑93 (MQ=255)

                                                   tgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCg                                       >  1:2690413/1‑93 (MQ=255)

                                                   tgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCg                                       >  2:1590962/1‑93 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      >  2:3219725/1‑93 (MQ=255)

                                                    gTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGt                                      >  1:2918108/1‑93 (MQ=255)

                                                     tttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGtt                                     <  2:4208275/93‑1 (MQ=255)

                                                        aaGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                  <  2:2483698/93‑1 (MQ=255)

                                                          gctgctGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                <  2:911537/93‑1 (MQ=255)

                                                             gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga                             >  2:4559066/1‑93 (MQ=255)

                                                              ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgat                            <  1:5452753/93‑1 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                           <  1:4891105/93‑1 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                           <  1:2779975/93‑1 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                           <  2:3945659/93‑1 (MQ=255)

                                                               tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACAATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                           >  2:4231317/1‑93 (MQ=255)

                                                                ggTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                          <  2:6093869/93‑1 (MQ=255)

                                                                ggTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                          >  1:6164980/1‑93 (MQ=255)

                                                                 gTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg                         <  2:2187428/93‑1 (MQ=255)

                                                                      aGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGa                    <  1:2404459/93‑1 (MQ=255)

                                                                      aGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGa                    >  2:857530/1‑93 (MQ=255)

                                                                       gCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAt                   <  2:3461103/93‑1 (MQ=255)

                                                                       gCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAt                   >  1:3686075/1‑93 (MQ=255)

                                                                         ggAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAttt                 >  2:2817482/1‑93 (MQ=255)

                                                                          gAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTg                <  1:371645/93‑1 (MQ=255)

                                                                           aaTAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGa               >  1:557863/1‑93 (MQ=255)

                                                                            aTAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt              >  2:3881896/1‑93 (MQ=255)

                                                                            aTAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt              <  2:2907367/93‑1 (MQ=255)

                                                                            aTAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAt              >  1:3866521/1‑93 (MQ=255)

                                                                             tAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAtt             >  1:4474532/1‑93 (MQ=255)

                                                                              aaaGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTg            >  2:742322/1‑93 (MQ=255)

                                                                              aaaGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTg            >  2:5282525/1‑93 (MQ=255)

                                                                               aaGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGt           >  2:4321947/1‑93 (MQ=255)

                                                                               aaGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGt           <  2:364770/93‑1 (MQ=255)

                                                                                aGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc          >  2:2676236/1‑93 (MQ=255)

                                                                                aGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc          >  2:6112465/1‑93 (MQ=255)

                                                                                 ggAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTcc         <  2:1921152/93‑1 (MQ=255)

                                                                                  gAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt        >  1:583904/1‑93 (MQ=255)

                                                                                  gAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt        <  1:985589/93‑1 (MQ=255)

                                                                                   aGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc       <  1:4777581/93‑1 (MQ=255)

                                                                                   aGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc       >  1:6317473/1‑93 (MQ=255)

                                                                                    gCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGAATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg      <  1:5355659/93‑1 (MQ=255)

                                                                                     ccGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCgg     >  2:4151850/1‑93 (MQ=255)

                                                                                       gATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCt   >  2:1618990/1‑93 (MQ=255)

                                                                                        aTAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  <  1:5145564/93‑1 (MQ=255)

                                                                                        aTAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  <  2:49285/93‑1 (MQ=255)

                                                                                        aTAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  1:1122849/1‑93 (MQ=255)

                                                                                        aTAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  2:4649735/1‑93 (MQ=255)

                                                                                        aTAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTg  >  1:3820468/1‑93 (MQ=255)

AGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG  >  NC_000913/3123490‑3123669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCA  >  NC_000913/3123492‑3123678

TTCCGCGTACATGCGACCTGGGTAAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG                                                                                                <  SRR4302547.726946/94‑1 (MQ=60)

TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG                                                                                                <  SRR4302547.4194754/94‑1 (MQ=60)

TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG                                                                                                <  SRR4302547.3378631/94‑1 (MQ=60)

TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG                                                                                                <  SRR4302547.3274372/94‑1 (MQ=60)

TTCCCCGTACATGCGACCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG                                                                                                <  SRR4302547.984655/94‑1 (MQ=60)

GTCCGCGTACATGCGACCGGGCTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG                                                                                                <  SRR4302547.4142891/94‑1 (MQ=60)

 TCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG‑G                                                                                              >  SRR4302547.5747698/1‑94 (MQ=60)

 TCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG‑G                                                                                              <  SRR4302547.4099804/94‑1 (MQ=60)

   CGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGgca                                                                                             <  SRR4302547.1020781/94‑4 (MQ=60)

     CGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAA                                                                                           >  SRR4302547.1514720/1‑94 (MQ=60)

      GTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAG                                                                                          >  SRR4302547.1476761/1‑94 (MQ=60)

      GTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAG                                                                                          >  SRR4302547.1043072/1‑94 (MQ=60)

          ATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCAC                                                                                      <  SRR4302547.5461820/94‑1 (MQ=60)

           TGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACC                                                                                     <  SRR4302547.4441617/94‑1 (MQ=60)

             GGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAT                                                                                   <  SRR4302547.2141458/94‑1 (MQ=60)

             CGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAT                                                                                   <  SRR4302547.2710737/94‑1 (MQ=60)

             CGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAT                                                                                   >  SRR4302547.2386283/1‑94 (MQ=60)

              GACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATC                                                                                  <  SRR4302547.5856375/94‑1 (MQ=60)

               ACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCT                                                                                 >  SRR4302547.5623496/1‑94 (MQ=60)

               ACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCT                                                                                 >  SRR4302547.6100514/1‑94 (MQ=60)

                CCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTC                                                                                >  SRR4302547.6142952/1‑94 (MQ=60)

                CCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTC                                                                                >  SRR4302547.2204240/1‑94 (MQ=60)

                 CGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCC                                                                               <  SRR4302547.2459017/94‑1 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCG                                                                              >  SRR4302547.2999076/1‑94 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCG                                                                              >  SRR4302547.959563/1‑94 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCG                                                                              <  SRR4302547.1167537/94‑1 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCG                                                                              >  SRR4302547.281977/1‑94 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCG                                                                              >  SRR4302547.3685409/1‑94 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTACG                                                                              >  SRR4302547.2999077/1‑94 (MQ=60)

                  GGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCAACCCCG                                                                              >  SRR4302547.2056433/1‑94 (MQ=60)

                    GGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGC                                                                            <  SRR4302547.3789717/94‑1 (MQ=60)

                    GGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGC                                                                            <  SRR4302547.5126851/94‑1 (MQ=60)

                    GGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGC                                                                            >  SRR4302547.2503007/1‑94 (MQ=60)

                        AAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTA                                                                        >  SRR4302547.4409065/1‑94 (MQ=60)

                        AAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTA                                                                        >  SRR4302547.2241319/1‑94 (MQ=60)

                        AAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTA                                                                        >  SRR4302547.401896/1‑94 (MQ=60)

                         AACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAA                                                                       <  SRR4302547.3772258/94‑1 (MQ=60)

                          ACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAA                                                                      >  SRR4302547.6205428/1‑94 (MQ=60)

                          ACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGCCAAAGCCACCATCTCCGGCACTAAA                                                                      <  SRR4302547.1228067/94‑1 (MQ=60)

                           CACGCCGCAAGGGTGGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAG                                                                     <  SRR4302547.2911897/94‑1 (MQ=60)

                           CACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAG                                                                     <  SRR4302547.4205181/94‑1 (MQ=60)

                            ACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGC                                                                    <  SRR4302547.5593811/94‑1 (MQ=60)

                            ACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGC                                                                    >  SRR4302547.4165583/1‑94 (MQ=60)

                            ACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGC                                                                    >  SRR4302547.5567297/1‑94 (MQ=60)

                               CCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGG                                                                 <  SRR4302547.5324829/94‑1 (MQ=60)

                                CGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGT                                                                >  SRR4302547.2782155/1‑94 (MQ=60)

                                 GCAGGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTC                                                               <  SRR4302547.3863877/94‑1 (MQ=60)

                                  CAAGGTTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCG                                                              <  SRR4302547.4037530/94‑1 (MQ=60)

                                   AAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGC                                                             >  SRR4302547.897325/1‑94 (MQ=60)

                                   AAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGC                                                             >  SRR4302547.340395/1‑94 (MQ=60)

                                    AGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCA                                                            <  SRR4302547.3934606/94‑1 (MQ=60)

                                    AGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGGCGCA                                                            >  SRR4302547.3983942/1‑94 (MQ=60)

                                       GTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGA                                                         >  SRR4302547.5390808/1‑94 (MQ=60)

                                        TCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGGTAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAC                                                        >  SRR4302547.54876/1‑94 (MQ=60)

                                        TCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAC                                                        <  SRR4302547.492159/94‑1 (MQ=60)

                                        TCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAC                                                        <  SRR4302547.1041176/94‑1 (MQ=60)

                                         CGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                       >  SRR4302547.5821030/1‑94 (MQ=60)

                                         CGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                       <  SRR4302547.1593954/94‑1 (MQ=60)

                                         CGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                       <  SRR4302547.5731181/94‑1 (MQ=60)

                                          GAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCG                                                      >  SRR4302547.4276398/1‑94 (MQ=60)

                                           AGCTGCTGTTTAAGCTGCTTGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGC                                                     >  SRR4302547.5920585/1‑94 (MQ=60)

                                           AGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGC                                                     >  SRR4302547.632783/1‑94 (MQ=60)

                                            GCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCC                                                    <  SRR4302547.5464622/94‑1 (MQ=60)

                                             CTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCA                                                   >  SRR4302547.5449270/1‑94 (MQ=60)

                                              TGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAG                                                  >  SRR4302547.4969555/1‑94 (MQ=60)

                                              TGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAG                                                  >  SRR4302547.4557075/1‑94 (MQ=60)

                                              TGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAACGGGTCGCATTACCGCCAG                                                  >  SRR4302547.5801110/1‑94 (MQ=60)

                                               actgtagaaggtcctgtaaATAGCGGATTAAAGGAGCCGATAGCGGGG‑AAAAGCCACCATGTCCGACAGTAAAGCGGGTCGCATGACCCCCGGC                                                 <  SRR4302547.2165886/75‑1 (MQ=32)

                                               GCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGC                                                 <  SRR4302547.6707178/94‑1 (MQ=60)

                                               GCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGC                                                 <  SRR4302547.3317928/94‑1 (MQ=60)

                                               GCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGC                                                 >  SRR4302547.5604122/1‑94 (MQ=60)

                                                CTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCG                                                >  SRR4302547.2744421/1‑94 (MQ=60)

                                                 TGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGT                                               >  SRR4302547.2976306/1‑94 (MQ=60)

                                                 TGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGT                                               >  SRR4302547.2211108/1‑94 (MQ=60)

                                                  GTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTT                                              >  SRR4302547.4156206/1‑94 (MQ=60)

                                                  GTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTT                                              >  SRR4302547.6435946/1‑94 (MQ=60)

                                                   TTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGAGTTG                                             >  SRR4302547.1580451/1‑94 (MQ=60)

                                                     TAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCG                                           >  SRR4302547.5442346/1‑94 (MQ=60)

                                                      AAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGG                                          <  SRR4302547.4902806/94‑1 (MQ=60)

                                                       AGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGG                                         >  SRR4302547.4874414/1‑94 (MQ=60)

                                                         CTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCG                                       >  SRR4302547.5305437/1‑94 (MQ=60)

                                                          TGCTGGTGATAGTGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGA                                      >  SRR4302547.873805/1‑94 (MQ=60)

                                                            CTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATG                                    <  SRR4302547.5565429/94‑1 (MQ=60)

                                                             TGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGC                                   <  SRR4302547.2835672/94‑1 (MQ=60)

                                                             TGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGC                                   <  SRR4302547.4992406/94‑1 (MQ=60)

                                                             TGGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGC                                   >  SRR4302547.6292939/1‑94 (MQ=60)

                                                              GGTGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCG                                  >  SRR4302547.396178/1‑94 (MQ=60)

                                                               GTGATAGCGGAAAAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGA                                 <  SRR4302547.3435088/94‑1 (MQ=60)

                                                                TGATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGAT                                >  SRR4302547.1212964/1‑94 (MQ=60)

                                                                  ATAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGG                              <  SRR4302547.5370863/94‑1 (MQ=60)

                                                                   TAGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGA                             >  SRR4302547.6675437/1‑94 (MQ=60)

                                                                    AGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAT                            <  SRR4302547.2453146/94‑1 (MQ=60)

                                                                    AGCGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAT                            >  SRR4302547.3761327/1‑94 (MQ=60)

                                                                      CGGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTT                          >  SRR4302547.3456944/1‑94 (MQ=60)

                                                                       GGAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTTGCATGACAGCCAGCGctgcagccgcagcgatggagtag                         >  SRR4302547.970492/1‑71 (MQ=60)

                                                                        GAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGG                        <  SRR4302547.322060/94‑1 (MQ=60)

                                                                        GAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGA                        >  SRR4302547.567917/1‑94 (MQ=60)

                                                                        GAATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGA                        <  SRR4302547.378534/94‑1 (MQ=60)

                                                                         AATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAT                       >  SRR4302547.3945233/1‑94 (MQ=60)

                                                                          ATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATT                      >  SRR4302547.4566977/1‑94 (MQ=60)

                                                                          ATAAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATT                      <  SRR4302547.1136802/94‑1 (MQ=60)

                                                                            AAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGT                    <  SRR4302547.1265449/94‑1 (MQ=60)

                                                                            AAAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATCTGATTGT                    >  SRR4302547.6411660/1‑94 (MQ=60)

                                                                             AAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTC                   <  SRR4302547.5260590/94‑1 (MQ=60)

                                                                             AAGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTC                   <  SRR4302547.6257096/94‑1 (MQ=60)

                                                                              AGGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCC                  <  SRR4302547.2927517/94‑1 (MQ=60)

                                                                               GGAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCT                 >  SRR4302547.594407/1‑94 (MQ=60)

                                                                                GAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTC                >  SRR4302547.6448839/1‑94 (MQ=60)

                                                                                GAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTC                <  SRR4302547.1004763/94‑1 (MQ=60)

                                                                                GAGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATAGTCCTC                <  SRR4302547.3128364/94‑1 (MQ=60)

                                                                                 AGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCG               >  SRR4302547.2654305/1‑94 (MQ=60)

                                                                                 AGCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCG               <  SRR4302547.4876548/94‑1 (MQ=60)

                                                                                  GCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGG              <  SRR4302547.2771512/94‑1 (MQ=60)

                                                                                  GCCGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGAATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGG              <  SRR4302547.5466101/94‑1 (MQ=60)

                                                                                    CGATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCT            >  SRR4302547.3293052/1‑94 (MQ=60)

                                                                                    CGATAGC‑GGGCCAAAGCCACAATCCCCGGCATTAAAGTGCATCGCATAACCCCCAGCGTTGCGGGCGATGCGATGGATTTGATTGGCCTCGGGT            <  SRR4302547.5028157/94‑1 (MQ=32)

                                                                                     GATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG           >  SRR4302547.1145495/1‑94 (MQ=60)

                                                                                     GATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG           >  SRR4302547.3898314/1‑94 (MQ=60)

                                                                                     GATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG           >  SRR4302547.6783334/1‑94 (MQ=60)

                                                                                      ATAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGT          <  SRR4302547.5251547/94‑1 (MQ=60)

                                                                                      ATAGCGGGG‑AAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGT          >  SRR4302547.6611947/1‑94 (MQ=60)

                                                                                       TAGCGGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTC         >  SRR4302547.511714/1‑94 (MQ=60)

                                                                                         gcgGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGA       <  SRR4302547.2411054/91‑1 (MQ=60)

                                                                                          cgGGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGAT      >  SRR4302547.1387837/3‑94 (MQ=60)

                                                                                          G‑GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGACGATGCGATGGATTTGATTGTCCTCGGCTGTCGATT     <  SRR4302547.5506047/94‑1 (MQ=60)

                                                                                            GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTT    >  SRR4302547.4196141/1‑94 (MQ=60)

                                                                                            GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTT    <  SRR4302547.1964622/94‑1 (MQ=60)

                                                                                              G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCA  >  SRR4302547.3550594/1‑94 (MQ=60)

TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCA  >  NC_000913/3123492‑3123678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`