Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,430,453	+C	coding (745/747 nt)	smf ←	putative Rossmann‑fold nucleotide‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,430,453	1	.	C	99.6%	807.0 / NA	222	P249A (CCT→GCT)	smf	putative Rossmann‑fold nucleotide‑binding protein
Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (132/89); minor base A (0/1); total (132/90)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑G‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430362‑3430539

 aTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAg‑gca                                                                                       <  2:2308668/93‑3 (MQ=255)

  tttCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaag‑g‑caa                                                                                     >  2:5178821/1‑93 (MQ=255)

  tttCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaag‑g‑caa                                                                                     <  1:817540/93‑1 (MQ=255)

    tcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑G‑caaaa                                                                                   >  2:3271076/1‑93 (MQ=255)

    tcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑G‑caaaa                                                                                   <  2:3807994/93‑1 (MQ=255)

     cttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑G‑caaaaa                                                                                  >  1:1268297/1‑92 (MQ=255)

      ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑G‑caaaaac                                                                                 >  2:862398/1‑91 (MQ=255)

       tcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACt                                                                                 >  2:5676710/1‑93 (MQ=255)

        ccGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACtt                                                                                >  1:4118489/1‑93 (MQ=255)

        ccGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACtt                                                                                <  2:4864813/93‑1 (MQ=255)

          ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAGCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAGCTtct                                                                              <  1:2770880/93‑1 (MQ=255)

          ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTtct                                                                              >  1:3520824/1‑93 (MQ=255)

          ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTtct                                                                              >  1:4870219/1‑93 (MQ=255)

           gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTtctc                                                                             <  2:4027837/93‑1 (MQ=255)

           gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTtctc                                                                             >  1:621393/1‑93 (MQ=255)

            ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCg                                                                            <  1:233089/93‑1 (MQ=255)

            ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCg                                                                            >  1:2450409/1‑93 (MQ=255)

               cGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGccc                                                                         <  1:2287794/93‑1 (MQ=255)

                gTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGcccc                                                                        <  2:4442509/93‑1 (MQ=255)

                 tCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCt                                                                       <  1:5969331/93‑1 (MQ=255)

                  cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTg                                                                      <  2:4140188/93‑1 (MQ=255)

                    caAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCt                                                                    <  1:931408/93‑1 (MQ=255)

                       gAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCaa                                                                 >  1:4193946/1‑93 (MQ=255)

                       gAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCaa                                                                 >  1:875595/1‑93 (MQ=255)

                        aaTCGCACCTTGTTTTATTAACCAGTGAGGTCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAg                                                                >  1:2116881/1‑93 (MQ=255)

                        aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAg                                                                <  2:731713/93‑1 (MQ=255)

                        aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAg                                                                >  2:1446645/1‑93 (MQ=255)

                         aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCGTCCCGGATTCCCTAGTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAgc                                                               <  2:6213561/93‑1 (MQ=255)

                          tCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAgca                                                              >  2:6379802/1‑92 (MQ=255)

                           cGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAgcgc                                                             >  1:2553743/1‑93 (MQ=255)

                            gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            >  1:388332/1‑93 (MQ=255)

                             cACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcac                                                           >  1:342601/1‑93 (MQ=255)

                             cACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcac                                                           >  1:6372562/1‑93 (MQ=255)

                             cACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcaa                                                           >  1:1930057/1‑92 (MQ=255)

                              aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  1:2242843/1‑93 (MQ=255)

                              aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  1:5296102/1‑93 (MQ=255)

                                cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAc                                                        >  1:6100413/1‑93 (MQ=255)

                                cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAc                                                        >  2:5848743/1‑93 (MQ=255)

                                cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAc                                                        >  1:4446714/1‑93 (MQ=255)

                                cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GAAAAAACTTCTCGCCCCTGCTCAAGCGCACAAc                                                        <  1:3595764/93‑1 (MQ=255)

                                 ttGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACg                                                       >  1:5086094/1‑93 (MQ=255)

                                  tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGt                                                      <  1:4667910/93‑1 (MQ=255)

                                   gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                     >  2:6433330/1‑93 (MQ=255)

                                   gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                     >  2:5309999/1‑93 (MQ=255)

                                    ttttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACCACGTGc                                                    >  1:4924073/1‑93 (MQ=255)

                                    ttttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGc                                                    >  1:5560909/1‑93 (MQ=255)

                                     tttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCt                                                   >  1:110985/1‑93 (MQ=255)

                                        attaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTc                                                >  1:5657376/1‑93 (MQ=255)

                                        attaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTc                                                >  1:2850993/1‑93 (MQ=255)

                                        atgaaCGAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTc                                                <  1:4330005/90‑1 (MQ=255)

                                           aaCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAcc                                             <  2:2692976/93‑1 (MQ=255)

                                            aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            <  2:483951/93‑1 (MQ=255)

                                             ccAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAg                                           >  1:6035905/1‑93 (MQ=255)

                                              cAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                          >  1:3186192/1‑93 (MQ=255)

                                               aGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCg                                         >  2:614991/1‑93 (MQ=255)

                                                   aGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAcc                                     >  2:5402370/1‑93 (MQ=255)

                                                   aGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAcc                                     <  2:5573376/93‑1 (MQ=255)

                                                    ggCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCa                                    >  1:3564988/1‑93 (MQ=255)

                                                       ccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTa                                 >  2:1179076/1‑93 (MQ=255)

                                                        cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  1:5035360/1‑93 (MQ=255)

                                                        cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  2:3036911/1‑93 (MQ=255)

                                                          tCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              <  1:4079836/93‑1 (MQ=255)

                                                            gCTTCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaa                            <  2:4478979/93‑1 (MQ=255)

                                                               tCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         <  1:5883050/93‑1 (MQ=255)

                                                               tCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         <  1:1803710/93‑1 (MQ=255)

                                                               tCCCGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         >  2:4282798/1‑93 (MQ=255)

                                                               tCCCGGATCCCCTATTGGACCGGGCAAG‑GCAAAACCTCCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         <  1:1194928/93‑1 (MQ=255)

                                                                cccTGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGcc                        <  2:68186/93‑1 (MQ=255)

                                                                cccGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGcc                        >  1:1169821/1‑93 (MQ=255)

                                                                cccGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGcc                        <  2:4605297/93‑1 (MQ=255)

                                                                  cGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGc                      <  1:5242516/93‑1 (MQ=255)

                                                                  cGGATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGc                      >  2:37000/1‑93 (MQ=255)

                                                                   ggATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGCACCACTACGCAAAGCAGCt                     >  1:3481645/1‑93 (MQ=255)

                                                                   ggATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                     <  2:5089372/93‑1 (MQ=255)

                                                                   ggATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                     >  2:2166881/1‑93 (MQ=255)

                                                                    gATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCtt                    >  2:1995507/1‑93 (MQ=255)

                                                                    gATTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCtt                    >  2:1048674/1‑93 (MQ=255)

                                                                     aTTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTc                   >  1:3955159/1‑93 (MQ=255)

                                                                     aTTCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTc                   >  1:6473230/1‑93 (MQ=255)

                                                                     aTTCCCGATGGGACCTGGCAAGTC‑AAGAGCTAGTCGCCCCTGCTCAAGCGCACAACGTGCTGTCCCCAGCGAACCACTACGCAAAGCCGCTTc                   <  2:5720284/93‑1 (MQ=255)

                                                                      ttCCCTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                  >  2:3373554/1‑93 (MQ=255)

                                                                        cccTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAGAGCCGCTTccac                >  1:1485864/1‑93 (MQ=255)

                                                                        cccTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccac                >  2:6197207/1‑93 (MQ=255)

                                                                        cccTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccac                >  2:5369448/1‑93 (MQ=255)

                                                                         ccTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacc               >  2:5921049/1‑93 (MQ=255)

                                                                         ccTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacc               >  2:29192/1‑93 (MQ=255)

                                                                         ccTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacc               >  1:6528180/1‑93 (MQ=255)

                                                                          cTATTGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacca              <  2:2731806/93‑1 (MQ=255)

                                                                          cTATTCGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTAACCAGCGAACCACTACGCAAAGCCGCTTccacgc              >  1:6573901/1‑91 (MQ=255)

                                                                             ttGGACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           <  2:1079752/93‑1 (MQ=255)

                                                                               ggACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGt         >  2:3524145/1‑93 (MQ=255)

                                                                                gACCTGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        >  2:343631/1‑93 (MQ=255)

                                                                                    tGGCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAcc    <  1:1550133/93‑1 (MQ=255)

                                                                                     ggcaag‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   <  2:5993012/93‑1 (MQ=255)

                                                                                     ggcaag‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   >  2:2642418/1‑93 (MQ=255)

                                                                                     ggcaag‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   <  1:5798894/93‑1 (MQ=255)

                                                                                     ggcaag‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   >  2:2553489/1‑93 (MQ=255)

                                                                                     ggcaag‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   <  2:4628317/93‑1 (MQ=255)

                                                                                     ggcaag‑gcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   <  2:1651010/93‑1 (MQ=255)

                                                                                      gCAAG‑GCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCtt  >  1:3746307/1‑93 (MQ=255)

AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAG‑G‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430362‑3430539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430361‑3430546

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑C                                                                                              >  SRR4302547.5191763/1‑94 (MQ=60)

  ATTTCTTCCGGTTCCGACACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAA                                                                                            <  SRR4302547.1567268/94‑1 (MQ=60)

   TTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAAA                                                                                           <  SRR4302547.4402973/94‑1 (MQ=60)

   TTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAAA                                                                                           <  SRR4302547.833064/94‑1 (MQ=60)

    TTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑CAAAA                                                                                          >  SRR4302547.783184/1‑94 (MQ=60)

     TCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAA                                                                                          >  SRR4302547.1293839/1‑94 (MQ=60)

      CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAAC                                                                                         <  SRR4302547.3622017/94‑1 (MQ=60)

       TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACT                                                                                        <  SRR4302547.2730751/94‑1 (MQ=60)

       TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACT                                                                                        <  SRR4302547.6572512/94‑1 (MQ=60)

       TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACT                                                                                        >  SRR4302547.4599413/1‑94 (MQ=60)

        TCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTT                                                                                       >  SRR4302547.4202194/1‑94 (MQ=60)

         CCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTC                                                                                      <  SRR4302547.2585383/94‑1 (MQ=60)

          CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCT                                                                                     >  SRR4302547.3592692/1‑94 (MQ=60)

          CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCT                                                                                     <  SRR4302547.862339/94‑1 (MQ=60)

          CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCT                                                                                     >  SRR4302547.4971143/1‑94 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAGCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAGCTTCTC                                                                                    <  SRR4302547.2826390/94‑1 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    >  SRR4302547.4209087/1‑94 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    >  SRR4302547.632883/1‑94 (MQ=60)

           GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTC                                                                                    <  SRR4302547.6360436/94‑1 (MQ=60)

            GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCG                                                                                   >  SRR4302547.2499920/1‑94 (MQ=60)

            GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCG                                                                                   >  SRR4302547.5623546/1‑94 (MQ=60)

            GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCG                                                                                   >  SRR4302547.4090673/1‑94 (MQ=60)

             TTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGC                                                                                  <  SRR4302547.237468/94‑1 (MQ=60)

               CCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCC                                                                                >  SRR4302547.5116971/1‑94 (MQ=60)

                CGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCC                                                                               >  SRR4302547.2088448/1‑94 (MQ=60)

                CGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCC                                                                               <  SRR4302547.2334111/94‑1 (MQ=60)

                 GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCT                                                                              <  SRR4302547.4965107/94‑1 (MQ=60)

                  TCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTG                                                                             <  SRR4302547.4455758/94‑1 (MQ=60)

                  TCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTG                                                                             <  SRR4302547.6093141/94‑1 (MQ=60)

                   CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGC                                                                            >  SRR4302547.5853221/1‑94 (MQ=60)

                   CACAAGAATCCCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATGGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGC                                                                            <  SRR4302547.2426975/94‑1 (MQ=60)

                    ACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCT                                                                           >  SRR4302547.6168817/1‑94 (MQ=60)

                    ACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCT                                                                           >  SRR4302547.5168642/1‑94 (MQ=60)

                     CAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTC                                                                          <  SRR4302547.949409/94‑1 (MQ=60)

                      AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCA                                                                         >  SRR4302547.2433222/1‑94 (MQ=60)

                       AGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAA                                                                        >  SRR4302547.892403/1‑94 (MQ=60)

                       AGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAA                                                                        >  SRR4302547.4279472/1‑94 (MQ=60)

                       AGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAA                                                                        >  SRR4302547.1375232/1‑94 (MQ=60)

                        GAATCGCACCTTGTTTTATTAACCAGTGAGGTCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAG                                                                       >  SRR4302547.2159071/1‑94 (MQ=60)

                         AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      <  SRR4302547.5348613/94‑1 (MQ=60)

                          ATCGCACCTTGTTTTATTAACCAGTGAGCCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCG                                                                     <  SRR4302547.1210882/94‑1 (MQ=60)

                           TCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGC                                                                    >  SRR4302547.2605313/1‑94 (MQ=60)

                           ACGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGC                                                                    <  SRR4302547.3456419/94‑1 (MQ=60)

                            CGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCA                                                                   >  SRR4302547.395534/1‑94 (MQ=60)

                             GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  >  SRR4302547.6505524/1‑94 (MQ=60)

                             GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  >  SRR4302547.6614783/1‑94 (MQ=60)

                             GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  >  SRR4302547.348963/1‑94 (MQ=60)

                             GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAA                                                                  >  SRR4302547.1968315/1‑94 (MQ=60)

                              CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 <  SRR4302547.602637/94‑1 (MQ=60)

                              CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 >  SRR4302547.5405370/1‑94 (MQ=60)

                              CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 >  SRR4302547.6123538/1‑94 (MQ=60)

                              CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 >  SRR4302547.2288075/1‑94 (MQ=60)

                               ACCTTGTTTTATTAACCAGTGAGGCGCTTCGCTTCCCGGATTCCCTATTGGACCTTGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302547.3664405/1‑94 (MQ=60)

                               ACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302547.6688100/1‑94 (MQ=60)

                               ACCTTGTTTGATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302547.6627202/1‑94 (MQ=60)

                                CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               <  SRR4302547.3175033/94‑1 (MQ=60)

                                CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               >  SRR4302547.6226947/1‑94 (MQ=60)

                                CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               >  SRR4302547.4538676/1‑94 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302547.5191007/1‑94 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302547.117814/94‑1 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302547.3630687/94‑1 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302547.3778459/94‑1 (MQ=60)

                                 CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGAAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302547.3669105/94‑1 (MQ=60)

                                   TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            <  SRR4302547.4764580/94‑1 (MQ=60)

                                   TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            <  SRR4302547.1776574/94‑1 (MQ=60)

                                   TGTTTTATTAACCAATAAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            <  SRR4302547.5575210/94‑1 (MQ=60)

                                    GTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACCACGTGC                                                           >  SRR4302547.5026070/1‑94 (MQ=60)

                                    GTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGC                                                           >  SRR4302547.5675603/1‑94 (MQ=60)

                                     TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          >  SRR4302547.113010/1‑94 (MQ=60)

                                       TTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGT                                                        <  SRR4302547.5016070/94‑1 (MQ=60)

                                        TATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTC                                                       >  SRR4302547.5774153/1‑94 (MQ=60)

                                        TATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTC                                                       >  SRR4302547.2907946/1‑94 (MQ=60)

                                         ATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCA                                                      <  SRR4302547.4419208/94‑1 (MQ=60)

                                         ATGAACGAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCA                                                      <  SRR4302547.4419098/94‑1 (MQ=60)

                                           TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACC                                                    >  SRR4302547.870225/1‑94 (MQ=60)

                                           TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACC                                                    >  SRR4302547.141427/1‑94 (MQ=60)

                                            AACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCA                                                   >  SRR4302547.293411/1‑94 (MQ=60)

                                             ACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAG                                                  >  SRR4302547.6161155/1‑94 (MQ=60)

                                             ACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAG                                                  <  SRR4302547.3507916/94‑1 (MQ=60)

                                              CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 >  SRR4302547.3249679/1‑94 (MQ=60)

                                               CAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCG                                                >  SRR4302547.4192657/1‑94 (MQ=60)

                                                 GTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAA                                              >  SRR4302547.2185131/1‑94 (MQ=60)

                                                   GAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACC                                            <  SRR4302547.5697912/94‑1 (MQ=60)

                                                    AGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCA                                           >  SRR4302547.3637732/1‑94 (MQ=60)

                                                    AGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCA                                           >  SRR4302547.1269645/1‑94 (MQ=60)

                                                     GGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCAC                                          >  SRR4302547.3558545/1‑94 (MQ=60)

                                                      GCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCACGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACgatt                                         >  SRR4302547.5583906/1‑90 (MQ=60)

                                                       CCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCTCACAACGTGCTGTCACCAGCGAACCACTA                                        >  SRR4302547.4101979/1‑94 (MQ=60)

                                                        CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302547.3980515/1‑94 (MQ=60)

                                                        CCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302547.5139375/1‑94 (MQ=60)

                                                         CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302547.4240201/1‑94 (MQ=60)

                                                         CTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      <  SRR4302547.579566/94‑1 (MQ=60)

                                                           TCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCA                                    <  SRR4302547.4162747/94‑1 (MQ=60)

                                                             GCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA                                  >  SRR4302547.5395728/1‑94 (MQ=60)

                                                               TTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGC                                >  SRR4302547.3939082/1‑94 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAACGCC                               >  SRR4302547.1203222/1‑94 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302547.5993267/94‑1 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302547.2312075/94‑1 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               >  SRR4302547.1193470/1‑94 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302547.6004850/94‑1 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302547.2829316/94‑1 (MQ=60)

                                                                TCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302547.1839374/94‑1 (MQ=60)

                                                                TCCCGGATCCCCTATTGGACCGGGCAAGGCAAAACCTCCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302547.1219042/94‑1 (MQ=60)

                                                                 CCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGttg                              >  SRR4302547.6036143/1‑91 (MQ=60)

                                                                   CGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGCACCACTACGCAAAGCAGCT                            >  SRR4302547.3552668/1‑94 (MQ=60)

                                                                   CGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            >  SRR4302547.6385885/1‑94 (MQ=60)

                                                                   CGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            <  SRR4302547.5350423/94‑1 (MQ=60)

                                                                    GGATTCCCTATTGGACCTGGGAAGGAAAAAACTTATCGCCCCTGCTCAAGCGCACAACGTGCTTTCATCAGCGAAACACTACGCACAGCCTCTT                           >  SRR4302547.2627010/1‑94 (MQ=60)

                                                                    GGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCAT                           >  SRR4302547.3791712/1‑94 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302547.6288912/1‑94 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          <  SRR4302547.1586738/94‑1 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302547.1974389/1‑94 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302547.4035606/1‑94 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302547.6608015/1‑94 (MQ=60)

                                                                     GATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302547.816437/1‑94 (MQ=60)

                                                                        TCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAGAGCCGCTTCCAC                       >  SRR4302547.1515539/1‑94 (MQ=60)

                                                                        TCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCAC                       >  SRR4302547.6165696/1‑94 (MQ=60)

                                                                         CCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACC                      >  SRR4302547.6664065/1‑94 (MQ=60)

                                                                         CCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACC                      >  SRR4302547.479564/1‑94 (MQ=60)

                                                                          CCTATTCGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTAACCAGCGAACCACTACGCAAAGCCGCTTCCACgc                     >  SRR4302547.6710767/1‑92 (MQ=60)

                                                                            TATTGGACCTGGCAAGGCAAAACCTCCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACC                   <  SRR4302547.2371302/94‑1 (MQ=60)

                                                                            TATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACC                   <  SRR4302547.91240/94‑1 (MQ=60)

                                                                             ATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCA                  >  SRR4302547.5029816/1‑94 (MQ=60)

                                                                               TGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGT                >  SRR4302547.35180/1‑94 (MQ=60)

                                                                                GGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               <  SRR4302547.2319436/94‑1 (MQ=60)

                                                                                     TGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          >  SRR4302547.1841396/1‑94 (MQ=60)

                                                                                     TGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          <  SRR4302547.1580995/94‑1 (MQ=60)

                                                                                      GGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT         <  SRR4302547.5918849/94‑1 (MQ=60)

                                                                                      GGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT         >  SRR4302547.3822782/1‑94 (MQ=60)

                                                                                       GCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        <  SRR4302547.4034709/94‑1 (MQ=60)

                                                                                       GCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTCCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        <  SRR4302547.2710982/94‑1 (MQ=60)

                                                                                        CAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       >  SRR4302547.4236982/1‑94 (MQ=60)

                                                                                        CAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       >  SRR4302547.743822/1‑94 (MQ=60)

                                                                                         AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGGCGCTTCCACTACCAGTACACCTTTAC      >  SRR4302547.4639074/1‑94 (MQ=60)

                                                                                         AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      <  SRR4302547.2841631/94‑1 (MQ=60)

                                                                                         AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTAC      >  SRR4302547.3380601/1‑94 (MQ=60)

                                                                                          AGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     <  SRR4302547.1118534/94‑1 (MQ=60)

                                                                                          AGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     >  SRR4302547.3258628/1‑94 (MQ=60)

                                                                                            C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302547.5628197/94‑1 (MQ=60)

                                                                                            C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302547.2093428/94‑1 (MQ=60)

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430361‑3430546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`