Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,580,830 T→C S54S (AGT→AGC)  yrhA → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,580,8300TC88.8% 414.7 / 37.3 143S54S (AGT→AGC) yrhAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (13/3);  new base C (99/28);  total (112/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 145 total aligned reads displayed.

GCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGAT  >  NC_000913/3580740‑3580922
                                                                                          |                                                                                            
gCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAt                                                                                            >  1:606517/1‑93 (MQ=255)
gCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAt                                                                                            <  2:6319689/93‑1 (MQ=255)
gCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAt                                                                                            >  1:6099818/1‑93 (MQ=255)
gCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAt                                                                                            >  1:4566956/1‑93 (MQ=255)
  tCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTc                                                                                          >  2:3521663/1‑93 (MQ=255)
   cAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCt                                                                                         >  2:2056999/1‑93 (MQ=255)
    aaGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCtt                                                                                        <  1:5598151/93‑1 (MQ=255)
    aaGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCtt                                                                                        >  2:5853515/1‑93 (MQ=255)
     aGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCttt                                                                                       <  1:3195325/93‑1 (MQ=255)
      ggAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCtttt                                                                                      >  1:3122373/1‑93 (MQ=255)
       gAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCttttt                                                                                     >  1:1147121/1‑93 (MQ=255)
         aTCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTaa                                                                                   >  2:4470487/1‑93 (MQ=255)
            aTTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGaa                                                                                >  2:4521994/1‑93 (MQ=255)
            aTTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGaa                                                                                >  1:6240777/1‑93 (MQ=255)
            aTTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGaa                                                                                >  1:5021399/1‑93 (MQ=255)
             tttATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAAt                                                                               >  1:4687728/1‑93 (MQ=255)
                 ttGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAAc                                                                           <  1:334721/93‑1 (MQ=255)
                  tGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAAcc                                                                          <  2:553140/93‑1 (MQ=255)
                   gAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCt                                                                         >  1:4324435/1‑93 (MQ=255)
                   gAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCt                                                                         >  2:3981656/1‑93 (MQ=255)
                      aGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGAt                                                                      >  1:5461925/1‑93 (MQ=255)
                        tataATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTa                                                                    >  1:3298280/1‑93 (MQ=255)
                        tataATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTa                                                                    >  1:3446642/1‑93 (MQ=255)
                         ataATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTAt                                                                   <  2:3603172/93‑1 (MQ=255)
                          taATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATc                                                                  <  1:22046/93‑1 (MQ=255)
                          taATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATc                                                                  >  2:5063561/1‑93 (MQ=255)
                            aTCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCtt                                                                >  1:1512635/1‑93 (MQ=255)
                            aTCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCtt                                                                >  2:5257508/1‑93 (MQ=255)
                             tCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCtaa                                                               >  2:1794022/1‑91 (MQ=255)
                               cAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACt                                                             <  2:3479636/93‑1 (MQ=255)
                                aaaTAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACtt                                                            >  2:3696838/1‑93 (MQ=255)
                                aaaTAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACtt                                                            >  1:3159465/1‑93 (MQ=255)
                                aaaTAGAATTTAATCCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACtt                                                            >  1:4393876/1‑93 (MQ=255)
                                 aaTAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACttt                                                           >  2:4577494/1‑93 (MQ=255)
                                 aaTAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACttt                                                           >  2:2862116/1‑93 (MQ=255)
                                 aaTAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACttt                                                           >  1:2402346/1‑93 (MQ=255)
                                   tAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACttttt                                                         >  1:2154868/1‑93 (MQ=255)
                                    aGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACtttttt                                                        >  1:1316385/1‑93 (MQ=255)
                                    aGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACtttttt                                                        <  1:5714039/93‑1 (MQ=255)
                                     gAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTa                                                       >  1:3275934/1‑93 (MQ=255)
                                     gAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTa                                                       >  1:2321492/1‑93 (MQ=255)
                                     gAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTa                                                       >  2:5679385/1‑93 (MQ=255)
                                      aaTTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTaa                                                      <  2:2402710/93‑1 (MQ=255)
                                       aTTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAag                                                     <  1:1008328/93‑1 (MQ=255)
                                         ttAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAagag                                                   <  1:1907996/93‑1 (MQ=255)
                                           aattctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCa                                                 <  2:4714455/93‑1 (MQ=255)
                                           aattctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCa                                                 >  2:5198474/1‑93 (MQ=255)
                                           aattctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCa                                                 >  2:1331653/1‑93 (MQ=255)
                                            attctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCaa                                                >  1:4911773/1‑93 (MQ=255)
                                            attctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCaa                                                >  2:3963514/1‑93 (MQ=255)
                                             ttctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAat                                               >  1:557563/1‑93 (MQ=255)
                                             ttctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAat                                               >  2:5766792/1‑93 (MQ=255)
                                             ttctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAat                                               <  2:1490356/93‑1 (MQ=255)
                                             ttctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAat                                               >  2:143187/1‑93 (MQ=255)
                                              tctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAatg                                              >  2:1534813/1‑93 (MQ=255)
                                               ctaattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAatgg                                             >  2:1213820/1‑93 (MQ=255)
                                                taattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAatgga                                            >  1:3003491/1‑93 (MQ=255)
                                                 aattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAatggat                                           >  1:6371963/1‑93 (MQ=255)
                                                 aattcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAatggat                                           >  1:3404195/1‑93 (MQ=255)
                                                  attcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAatggatg                                          >  1:1684812/1‑93 (MQ=255)
                                                    tcAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAAtggatgga                                        >  2:4242295/1‑93 (MQ=255)
                                                      aaCTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGAtt                                      >  2:3423401/1‑93 (MQ=255)
                                                      aaCTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGAtt                                      >  2:4107797/1‑93 (MQ=255)
                                                      aaCTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGAtt                                      >  2:782210/1‑93 (MQ=255)
                                                       aCTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTc                                     >  2:2409894/1‑93 (MQ=255)
                                                       aCTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTc                                     >  1:628152/1‑93 (MQ=255)
                                                       aCTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTc                                     >  1:3673748/1‑93 (MQ=255)
                                                           aTTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAg                                 >  2:1121749/1‑93 (MQ=255)
                                                           aTTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAg                                 >  1:5857361/1‑93 (MQ=255)
                                                             tGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGtt                               >  2:5390364/1‑93 (MQ=255)
                                                             tGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGtt                               <  1:1249804/93‑1 (MQ=255)
                                                             tGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGtt                               <  2:6186687/93‑1 (MQ=255)
                                                              gCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTa                              <  1:4354755/93‑1 (MQ=255)
                                                               ccTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTaa                             >  1:751636/1‑93 (MQ=255)
                                                               ccTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTaa                             >  2:4721461/1‑93 (MQ=255)
                                                                  ggAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATgg                          >  2:5270295/1‑93 (MQ=255)
                                                                  ggAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATgg                          >  1:4783838/1‑93 (MQ=255)
                                                                  ggAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATgg                          >  1:492428/1‑93 (MQ=255)
                                                                  ggAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATgg                          >  2:6056620/1‑93 (MQ=255)
                                                                  ggAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCCAAGTTAATgg                          >  1:3295918/1‑93 (MQ=255)
                                                                   gagaAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGa                         >  1:36953/1‑93 (MQ=255)
                                                                      aaGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTa                      <  2:6146869/93‑1 (MQ=255)
                                                                        gTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACg                    >  1:1039379/1‑93 (MQ=255)
                                                                           aTGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGatt                 >  1:4660996/1‑93 (MQ=255)
                                                                           aTGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGatt                 >  1:6261682/1‑93 (MQ=255)
                                                                            tGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGatta                >  1:4135823/1‑93 (MQ=255)
                                                                            tGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGatta                >  2:1627107/1‑93 (MQ=255)
                                                                             gTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGattat               >  2:4340645/1‑93 (MQ=255)
                                                                              tAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGattatt              >  1:1509580/1‑93 (MQ=255)
                                                                                aTGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTa            >  2:1922821/1‑93 (MQ=255)
                                                                                aTGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTa            >  1:6599130/1‑93 (MQ=255)
                                                                                aTGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTa            <  2:5795691/93‑1 (MQ=255)
                                                                                  gAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGc          <  1:2076218/93‑1 (MQ=255)
                                                                                   aaGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGcc         <  2:2235569/93‑1 (MQ=255)
                                                                                   aaGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGcc         >  1:1967085/1‑93 (MQ=255)
                                                                                     gTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCctc       >  1:6223948/1‑93 (MQ=255)
                                                                                      ttAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCctct      >  1:1785895/1‑93 (MQ=255)
                                                                                      ttAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCctct      >  2:2864208/1‑93 (MQ=255)
                                                                                       tAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCtctc     >  1:3349382/1‑93 (MQ=255)
                                                                                          tATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGAt  >  2:4358257/1‑93 (MQ=255)
                                                                                          |                                                                                            
GCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGAT  >  NC_000913/3580740‑3580922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATT  >  NC_000913/3580737‑3580923
                                                                                             |                                                                                             
ACCGCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGC                                                                                               <  SRR4302547.575531/94‑1 (MQ=60)
  CGCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAT                                                                                             >  SRR4302547.617791/1‑94 (MQ=60)
  CGCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAT                                                                                             >  SRR4302547.6226344/1‑94 (MQ=60)
  CGCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCAT                                                                                             >  SRR4302547.4661452/1‑94 (MQ=60)
    CTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTC                                                                                           >  SRR4302547.3591330/1‑94 (MQ=60)
       AAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTT                                                                                        <  SRR4302547.5713631/94‑1 (MQ=60)
        AGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTT                                                                                       >  SRR4302547.3386609/1‑94 (MQ=60)
        AGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTT                                                                                       <  SRR4302547.3258999/94‑1 (MQ=60)
        AGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTT                                                                                       >  SRR4302547.3184296/1‑94 (MQ=60)
         GGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTT                                                                                      >  SRR4302547.1170316/1‑94 (MQ=60)
             TCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGA                                                                                  >  SRR4302547.6374615/1‑94 (MQ=60)
              CATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAA                                                                                 >  SRR4302547.5125160/1‑94 (MQ=60)
              CATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAA                                                                                 >  SRR4302547.6370426/1‑94 (MQ=60)
               ATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAAT                                                                                >  SRR4302547.4784815/1‑94 (MQ=60)
                 TTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCA                                                                              >  SRR4302547.3883149/1‑94 (MQ=60)
                   ATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAAC                                                                            <  SRR4302547.2176639/94‑1 (MQ=60)
                    TTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACC                                                                           <  SRR4302547.340949/94‑1 (MQ=60)
                     TGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCT                                                                          >  SRR4302547.4413456/1‑94 (MQ=60)
                        AAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGAT                                                                       >  SRR4302547.5574759/1‑94 (MQ=60)
                          GTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTA                                                                     >  SRR4302547.3364649/1‑94 (MQ=60)
                          GTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTA                                                                     >  SRR4302547.3516908/1‑94 (MQ=60)
                          GTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTA                                                                     <  SRR4302547.3455992/94‑1 (MQ=60)
                           TATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTAT                                                                    >  SRR4302547.4875759/1‑94 (MQ=60)
                             TAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCT                                                                  <  SRR4302547.22474/94‑1 (MQ=60)
                              AATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTT                                                                 >  SRR4302547.1542778/1‑94 (MQ=60)
                                  CAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTT                                                             >  SRR4302547.3222313/1‑94 (MQ=60)
                                  CAAATAGAATTTAATCCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTT                                                             >  SRR4302547.4484304/1‑94 (MQ=60)
                                   AAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTT                                                            >  SRR4302547.2451000/1‑94 (MQ=60)
                                     ATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTT                                                          >  SRR4302547.2197904/1‑94 (MQ=60)
                                      TAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTT                                                         >  SRR4302547.1342848/1‑94 (MQ=60)
                                       AGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTA                                                        <  SRR4302547.5832054/94‑1 (MQ=60)
                                       AGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTA                                                        >  SRR4302547.3341586/1‑94 (MQ=60)
                                       AGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTA                                                        >  SRR4302547.2368547/1‑94 (MQ=60)
                                          ATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGA                                                     <  SRR4302547.1028016/94‑1 (MQ=60)
                                            TTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGC                                                   <  SRR4302547.1945751/94‑1 (MQ=60)
                                            TTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGC                                                   <  SRR4302547.2796644/94‑1 (MQ=60)
                                            TTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGC                                                   >  SRR4302547.6244907/1‑94 (MQ=60)
                                             TAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCA                                                  >  SRR4302547.3288472/1‑94 (MQ=60)
                                             TAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCA                                                  <  SRR4302547.1826798/94‑1 (MQ=60)
                                              AATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAA                                                 >  SRR4302547.5013483/1‑94 (MQ=60)
                                               ATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAAT                                                >  SRR4302547.567611/1‑94 (MQ=60)
                                                  CTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATCCTTTTTAAGAATCAACCTGATTCTCTTACTTTTTTAAGAGCAATGGA                                             <  SRR4302547.5450940/94‑1 (MQ=60)
                                                  CTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGA                                             >  SRR4302547.3063242/1‑94 (MQ=60)
                                                   TAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGAT                                            >  SRR4302547.3473469/1‑94 (MQ=60)
                                                   TAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGAT                                            >  SRR4302547.6504916/1‑94 (MQ=60)
                                                   TAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGAT                                            >  SRR4302547.4683904/1‑94 (MQ=60)
                                                    AATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATG                                           >  SRR4302547.1717970/1‑94 (MQ=60)
                                                       TCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGAT                                        >  SRR4302547.1213466/1‑94 (MQ=60)
                                                         AACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTC                                      >  SRR4302547.3748691/1‑94 (MQ=60)
                                                         AACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTC                                      <  SRR4302547.1666579/94‑1 (MQ=60)
                                                         AACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTC                                      >  SRR4302547.639747/1‑94 (MQ=60)
                                                             AATTGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAG                                  >  SRR4302547.5978556/1‑94 (MQ=60)
                                                                TGCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTA                               <  SRR4302547.1275023/94‑1 (MQ=60)
                                                                 GCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAA                              >  SRR4302547.765812/1‑94 (MQ=60)
                                                                 GCCTGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAA                              <  SRR4302547.4444214/94‑1 (MQ=60)
                                                                    TGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGG                           >  SRR4302547.501418/1‑94 (MQ=60)
                                                                    TGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGG                           >  SRR4302547.4882918/1‑94 (MQ=60)
                                                                    TGGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCCAAGTTAATGG                           >  SRR4302547.3362188/1‑94 (MQ=60)
                                                                     GGAGAAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGA                          >  SRR4302547.37636/1‑94 (MQ=60)
                                                                         AAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTAC                      >  SRR4302547.5022515/1‑94 (MQ=60)
                                                                         AAGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTAC                      <  SRR4302547.1357758/94‑1 (MQ=60)
                                                                          AGTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACG                     >  SRR4302547.1059857/1‑94 (MQ=60)
                                                                           GTTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGA                    >  SRR4302547.3191582/1‑94 (MQ=60)
                                                                            TTATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGAT                   <  SRR4302547.5981136/94‑1 (MQ=60)
                                                                             TATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATT                  >  SRR4302547.5811299/1‑94 (MQ=60)
                                                                             TATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATT                  >  SRR4302547.4757528/1‑94 (MQ=60)
                                                                             TATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATT                  >  SRR4302547.6391769/1‑94 (MQ=60)
                                                                              ATGTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTA                 >  SRR4302547.4219973/1‑94 (MQ=60)
                                                                                GTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATT               >  SRR4302547.2352228/1‑94 (MQ=60)
                                                                                GTAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATT               >  SRR4302547.1539667/1‑94 (MQ=60)
                                                                                 TAATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTT              >  SRR4302547.6734912/1‑94 (MQ=60)
                                                                                  AATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTA             >  SRR4302547.4461306/1‑94 (MQ=60)
                                                                                  AATGAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTA             >  SRR4302547.6736500/1‑94 (MQ=60)
                                                                                     GAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCC          <  SRR4302547.2117632/94‑1 (MQ=60)
                                                                                     GAAGTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCC          >  SRR4302547.2006158/1‑94 (MQ=60)
                                                                                       AGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTC        >  SRR4302547.6353209/1‑94 (MQ=60)
                                                                                        GTTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCT       >  SRR4302547.1821264/1‑94 (MQ=60)
                                                                                         TTAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTC      >  SRR4302547.3417298/1‑94 (MQ=60)
                                                                                          TAGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCG     >  SRR4302547.6140714/1‑94 (MQ=60)
                                                                                           AGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGA    >  SRR4302547.5352553/1‑94 (MQ=60)
                                                                                           AGCATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGA    >  SRR4302547.2256484/1‑94 (MQ=60)
                                                                                             CATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATT  >  SRR4302547.2623433/1‑94 (MQ=60)
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ACCGCTCAAGGAATCATTTATTGAAAGTATAATCCAAATAGAATTTAATTCTAATTCAACTAATTGCCTGGAGAAGTTATGTAATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATT  >  NC_000913/3580737‑3580923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑