Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,667,459	G→A	D80N (GAC→AAC)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,667,459	0	G	A	88.0%	699.7 / 60.8	233	D80N (GAC→AAC)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand): ref base G (16/12); new base A (114/91); total (130/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTT  >  NC_000913/3667367‑3667548

cctCTGCTGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCg                                                                                           >  2:1213756/1‑93 (MQ=255)

 ctctGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGa                                                                                          >  2:5009494/1‑93 (MQ=255)

  tctGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAAc                                                                                         >  1:1302422/1‑93 (MQ=255)

     gATGCGCTGTCGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGCCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTgc                                                                                      <  1:3839739/93‑1 (MQ=255)

     gATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTgc                                                                                      <  2:3083094/93‑1 (MQ=255)

     gATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTgc                                                                                      >  1:6359290/1‑93 (MQ=255)

      aTGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTgcg                                                                                     <  1:1819387/93‑1 (MQ=255)

        gcgcTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCa                                                                                   <  1:6151098/93‑1 (MQ=255)

           cTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCt                                                                                >  2:372396/1‑93 (MQ=255)

             gACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCCACTGCGCACCTaa                                                                              >  1:4999102/1‑93 (MQ=255)

              aCGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTaaa                                                                             >  1:3001633/1‑93 (MQ=255)

              aCGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAAACTTTCCCAACTGCGCACCTaaa                                                                             >  1:5766000/1‑93 (MQ=255)

               cGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTaaaa                                                                            <  2:2204520/93‑1 (MQ=255)

                 ccTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATg                                                                          <  1:2457929/93‑1 (MQ=255)

                  cTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATgg                                                                         >  2:2339982/1‑93 (MQ=255)

                   tGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAACCATTTCCAAACTGGGACCCTAAATTGGa                                                                        >  2:2857079/1‑93 (MQ=255)

                   tGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGa                                                                        <  1:2353429/93‑1 (MQ=255)

                    gCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGAc                                                                       <  1:1420632/93‑1 (MQ=255)

                     ccGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGAcc                                                                      <  2:5180701/93‑1 (MQ=255)

                          cGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTg                                                                 <  2:4810272/93‑1 (MQ=255)

                           gCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTgg                                                                >  1:85392/1‑93 (MQ=255)

                             tATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGat                                                              >  1:1527966/1‑93 (MQ=255)

                              aTCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGata                                                             >  2:289692/1‑93 (MQ=255)

                              aTCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGatc                                                             >  2:4566512/1‑92 (MQ=255)

                              aTCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGata                                                             >  2:443559/1‑93 (MQ=255)

                               tctcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGatat                                                            <  1:5739065/93‑1 (MQ=255)

                                ctcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATc                                                           <  1:4225184/93‑1 (MQ=255)

                                 tcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCt                                                          >  1:590844/1‑93 (MQ=255)

                                   gAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTa                                                        >  1:4886393/1‑93 (MQ=255)

                                   gAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTa                                                        >  1:3424463/1‑93 (MQ=255)

                                    aaCTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTaa                                                       >  1:4751885/1‑93 (MQ=255)

                                      cTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATc                                                     <  2:6306593/93‑1 (MQ=255)

                                      cTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATc                                                     <  1:1554613/93‑1 (MQ=255)

                                      cTGTTCGAGAATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATc                                                     <  2:5428579/93‑1 (MQ=255)

                                       tGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATcc                                                    >  1:6010614/1‑93 (MQ=255)

                                         ttCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGc                                                  >  2:1982919/1‑93 (MQ=255)

                                          tCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCt                                                 >  1:185348/1‑93 (MQ=255)

                                          tCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCt                                                 <  2:5804333/93‑1 (MQ=255)

                                            gAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAc                                               >  2:3860943/1‑93 (MQ=255)

                                            gAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAc                                               >  2:402320/1‑93 (MQ=255)

                                             aGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAcc                                              >  1:4936097/1‑93 (MQ=255)

                                             aGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAcc                                              >  1:1750312/1‑93 (MQ=255)

                                             aGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAcc                                              >  1:5208202/1‑93 (MQ=255)

                                               cATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGt                                            >  2:6607845/1‑93 (MQ=255)

                                                 tGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTaa                                          <  2:2393723/93‑1 (MQ=255)

                                                   ttCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAg                                        <  1:4089120/93‑1 (MQ=255)

                                                   ttCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAg                                        <  2:4715682/93‑1 (MQ=255)

                                                     cAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATACGCTACCGTAAAGTg                                      >  1:2861104/1‑93 (MQ=255)

                                                      aGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgc                                     <  2:5444050/93‑1 (MQ=255)

                                                      aGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgc                                     <  1:5386508/93‑1 (MQ=255)

                                                      aGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgc                                     >  2:1241173/1‑93 (MQ=255)

                                                       gTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgcg                                    >  2:4316395/1‑93 (MQ=255)

                                                       gTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgcg                                    >  2:3255109/1‑93 (MQ=255)

                                                       gTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgcg                                    >  2:1537470/1‑93 (MQ=255)

                                                        tCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgcgc                                   >  1:758354/1‑93 (MQ=255)

                                                        tCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgcgc                                   >  1:3128455/1‑93 (MQ=255)

                                                         cGGCGAAAATTTTCCCCGACAGTAAATCCTTTCCCAACTGCGCACCTAAAATGGCCCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGcc                                  <  2:2172509/93‑1 (MQ=255)

                                                         cGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGcc                                  >  1:3369740/1‑93 (MQ=255)

                                                           gCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGt                                <  1:3624632/93‑1 (MQ=255)

                                                              aaaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAt                             <  2:1777682/93‑1 (MQ=255)

                                                              aaaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAt                             <  2:409933/93‑1 (MQ=255)

                                                              aaaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAt                             <  1:5089266/93‑1 (MQ=255)

                                                              aaaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAt                             <  2:3022878/93‑1 (MQ=255)

                                                              aaaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAt                             >  1:3086145/1‑93 (MQ=255)

                                                              aaaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCATAAAGTGCGCCGTCAt                             >  1:688391/1‑93 (MQ=255)

                                                               aaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATc                            <  2:6294660/93‑1 (MQ=255)

                                                               aaaTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATc                            <  1:459020/93‑1 (MQ=255)

                                                                 aTTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGt                          <  1:5568723/93‑1 (MQ=255)

                                                                      ccccGACAGTAAACCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGAttt                     <  2:2739589/93‑1 (MQ=255)

                                                                       cccGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGAtttt                    >  2:5674868/1‑93 (MQ=255)

                                                                         cGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGa                  >  2:3594313/1‑93 (MQ=255)

                                                                          gACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGAc                 <  2:6059647/93‑1 (MQ=255)

                                                                          gACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGAc                 >  1:1033040/1‑93 (MQ=255)

                                                                           aCAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACt                >  1:2615013/1‑93 (MQ=255)

                                                                             aGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTg              >  2:3766479/1‑93 (MQ=255)

                                                                               tAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTgcg            >  1:3932722/1‑93 (MQ=255)

                                                                               tAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTgcg            >  2:1404085/1‑93 (MQ=255)

                                                                                aaaaCCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTgcgc           >  1:6529994/1‑93 (MQ=255)

                                                                                 aaaCCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCa          <  1:2212884/93‑1 (MQ=255)

                                                                                 aaaCCTTTCCCAACTTCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCa          <  1:4402557/93‑1 (MQ=255)

                                                                                  acccTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCaa         <  1:2629736/91‑1 (MQ=255)

                                                                                  aaCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCaa         >  1:4242679/1‑93 (MQ=255)

                                                                                  aaCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCaa         <  2:2485337/93‑1 (MQ=255)

                                                                                  aaCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGAGCGCCGTCATCGCGATTTTGACTTGCGCaa         >  1:593447/1‑93 (MQ=255)

                                                                                   aCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAg        <  1:923273/93‑1 (MQ=255)

                                                                                   aCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAg        >  2:3499769/1‑93 (MQ=255)

                                                                                    ccTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGt       <  1:6217589/93‑1 (MQ=255)

                                                                                     cTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGtt      <  2:1101077/93‑1 (MQ=255)

                                                                                     cTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGtt      >  2:6121302/1‑93 (MQ=255)

                                                                                     cTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGtt      <  2:1285443/93‑1 (MQ=255)

                                                                                      tttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGttt     >  1:5292773/1‑93 (MQ=255)

                                                                                      tttCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGttt     <  2:52603/93‑1 (MQ=255)

                                                                                      tttCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGttt     >  1:3095905/1‑93 (MQ=255)

                                                                                      tttCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGttt     >  2:6149642/1‑93 (MQ=255)

                                                                                      tttCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGttt     >  2:6225051/1‑93 (MQ=255)

                                                                                       ttCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttg    >  2:6432856/1‑93 (MQ=255)

                                                                                        tCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgt   <  2:268774/93‑1 (MQ=255)

                                                                                        tCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgt   <  1:3993120/93‑1 (MQ=255)

                                                                                        tCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgt   >  2:1422315/1‑93 (MQ=255)

                                                                                         cccAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATCTTGACTTGCGCAAGTttgtt  <  1:6503922/93‑1 (MQ=255)

CCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTT  >  NC_000913/3667367‑3667548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAA  >  NC_000913/3667366‑3667551

GCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCA                                                                                              <  SRR4302547.1607645/94‑1 (MQ=60)

 CCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAA                                                                                             >  SRR4302547.530757/1‑94 (MQ=60)

 CCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAA                                                                                             <  SRR4302547.5829184/94‑1 (MQ=60)

  CTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAAC                                                                                            >  SRR4302547.1328626/1‑94 (MQ=60)

   TCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACT                                                                                           <  SRR4302547.2655106/94‑1 (MQ=60)

   TCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACT                                                                                           >  SRR4302547.112397/1‑94 (MQ=60)

     TGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGC                                                                                         >  SRR4302547.6491924/1‑94 (MQ=60)

      GATGCGCTGTCGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGCCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCG                                                                                        <  SRR4302547.3917941/94‑1 (MQ=60)

      GATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCG                                                                                        <  SRR4302547.5119276/94‑1 (MQ=60)

       GTGCGCTGCCGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGC                                                                                       <  SRR4302547.6537793/94‑1 (MQ=60)

       ATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGC                                                                                       <  SRR4302547.1855351/94‑1 (MQ=60)

         GCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCAC                                                                                     >  SRR4302547.1846794/1‑94 (MQ=60)

         GCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCAC                                                                                     <  SRR4302547.6278725/94‑1 (MQ=60)

            CTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTA                                                                                  >  SRR4302547.4430335/1‑94 (MQ=60)

            CTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTA                                                                                  <  SRR4302547.3172338/94‑1 (MQ=60)

            CTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTA                                                                                  >  SRR4302547.1478279/1‑94 (MQ=60)

             TGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCCACTGCGCACCTAA                                                                                 >  SRR4302547.5102458/1‑94 (MQ=60)

              GACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAA                                                                                >  SRR4302547.3061320/1‑94 (MQ=60)

              GACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAAACTTTCCCAACTGCGCACCTAAA                                                                                >  SRR4302547.5885248/1‑94 (MQ=60)

                CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAAT                                                                              >  SRR4302547.728831/1‑94 (MQ=60)

                CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAAT                                                                              >  SRR4302547.1677191/1‑94 (MQ=60)

                CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCAAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAAT                                                                              <  SRR4302547.3089617/94‑1 (MQ=60)

                  CCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGG                                                                            <  SRR4302547.3835596/94‑1 (MQ=60)

                  CCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGG                                                                            <  SRR4302547.2507598/94‑1 (MQ=60)

                   CTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGA                                                                           >  SRR4302547.5318600/1‑94 (MQ=60)

                   CTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGA                                                                           >  SRR4302547.3147729/1‑94 (MQ=60)

                   CTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGA                                                                           >  SRR4302547.2401536/1‑94 (MQ=60)

                    TGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGAC                                                                          <  SRR4302547.2401117/94‑1 (MQ=60)

                    TGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGAC                                                                          >  SRR4302547.430048/1‑94 (MQ=60)

                     GCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACC                                                                         <  SRR4302547.1449071/94‑1 (MQ=60)

                       CGATCTCTATCTCAAACTGTTCGAGCATGTTCAGTCGGCAAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCG                                                                       <  SRR4302547.3550011/94‑1 (MQ=60)

                       CGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGGGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCG                                                                       >  SRR4302547.40115/1‑94 (MQ=60)

                       CGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCG                                                                       >  SRR4302547.6259945/1‑94 (MQ=60)

                       CGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCG                                                                       <  SRR4302547.860737/94‑1 (MQ=60)

                       CGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCG                                                                       <  SRR4302547.1494455/94‑1 (MQ=60)

                       CGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCC                                                                       >  SRR4302547.4026654/1‑94 (MQ=60)

                        GATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGC                                                                      >  SRR4302547.2425441/1‑94 (MQ=60)

                         ATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAACTTTTCCCCGACAGTAAAACCTTTCCCGCCTGCGCACCTAAAATTGACCCGCT                                                                     >  SRR4302547.1674255/1‑94 (MQ=60)

                           CGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGG                                                                   >  SRR4302547.86970/1‑94 (MQ=60)

                            GCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGA                                                                  <  SRR4302547.2228981/94‑1 (MQ=60)

                            GCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGA                                                                  <  SRR4302547.2043521/94‑1 (MQ=60)

                             CTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGCAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGAT                                                                 >  SRR4302547.4762702/1‑94 (MQ=60)

                             CTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGAT                                                                 >  SRR4302547.1558382/1‑94 (MQ=60)

                              TATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATA                                                                >  SRR4302547.1702113/1‑94 (MQ=60)

                              TATCTCGAACTGTGCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATA                                                                <  SRR4302547.6053263/94‑1 (MQ=60)

                                TCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATC                                                              <  SRR4302547.5857665/94‑1 (MQ=60)

                                 ctcatattattcgagtatatccagtcggccaaatgtttcaCCGACAGTAAAACCTTACCCAACTGCGCCCCTAAAATGGACCCGCTGGATATTT                                                             <  SRR4302547.1995587/54‑1 (MQ=47)

                                 CTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAAGCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCT                                                             >  SRR4302547.5851478/1‑94 (MQ=60)

                                 CTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCT                                                             >  SRR4302547.79213/1‑94 (MQ=60)

                                 CTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCT                                                             >  SRR4302547.5071023/1‑94 (MQ=60)

                                 CTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCT                                                             >  SRR4302547.601579/1‑94 (MQ=60)

                                 CTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCT                                                             <  SRR4302547.4311513/94‑1 (MQ=60)

                                 CTCGAACTGTCCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAACCCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCT                                                             <  SRR4302547.6058089/94‑1 (MQ=60)

                                   CGAACTGTTCGGGCATGTTCAGTCGGCGAAAATTTTTCCCGACAGTAAAACCTTTCCTAACTGCGCACCTTAAATGGACCCGCTGGATATCTTA                                                           >  SRR4302547.3094488/1‑94 (MQ=60)

                                   CGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTA                                                           >  SRR4302547.3494195/1‑94 (MQ=60)

                                   CGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTA                                                           >  SRR4302547.4987615/1‑94 (MQ=60)

                                    GAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAA                                                          >  SRR4302547.4850361/1‑94 (MQ=60)

                                     aaccgctcttccgatcttgacattCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAAT                                                         >  SRR4302547.6268826/25‑94 (MQ=60)

                                      ACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAAACTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATC                                                        >  SRR4302547.614823/1‑94 (MQ=60)

                                       CTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCC                                                       >  SRR4302547.6135361/1‑94 (MQ=60)

                                       CTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCC                                                       <  SRR4302547.4874007/94‑1 (MQ=60)

                                       CTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCC                                                       <  SRR4302547.1585546/94‑1 (MQ=60)

                                          TTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCT                                                    >  SRR4302547.188834/1‑94 (MQ=60)

                                            CGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAC                                                  >  SRR4302547.5858590/1‑94 (MQ=60)

                                             GAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACC                                                 >  SRR4302547.5315380/1‑94 (MQ=60)

                                             GAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACC                                                 <  SRR4302547.3218992/94‑1 (MQ=60)

                                             GAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACC                                                 >  SRR4302547.1784868/1‑94 (MQ=60)

                                             GAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACC                                                 >  SRR4302547.5038354/1‑94 (MQ=60)

                                                CATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTA                                              >  SRR4302547.530675/1‑94 (MQ=60)

                                                 ATTTTCAGTCGACGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAA                                             <  SRR4302547.3450181/94‑1 (MQ=60)

                                                  TGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAA                                            >  SRR4302547.2782815/1‑94 (MQ=60)

                                                  TGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAA                                            >  SRR4302547.2709107/1‑94 (MQ=60)

                                                  TGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTAGATATCTTAATCCGCTACCGTAAA                                            >  SRR4302547.6657583/1‑94 (MQ=60)

                                                   GTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAG                                           >  SRR4302547.1338051/1‑94 (MQ=60)

                                                    TTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGT                                          <  SRR4302547.886672/94‑1 (MQ=60)

                                                    TTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGT                                          >  SRR4302547.6018846/1‑94 (MQ=60)

                                                    TTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGT                                          <  SRR4302547.4172267/94‑1 (MQ=60)

                                                     TCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATACGCTACCGTAAAGTG                                         >  SRR4302547.2918257/1‑94 (MQ=60)

                                                      CAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGC                                        <  SRR4302547.4599899/94‑1 (MQ=60)

                                                       AGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCG                                       >  SRR4302547.3965066/1‑94 (MQ=60)

                                                       AGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCG                                       <  SRR4302547.5497733/94‑1 (MQ=60)

                                                        GTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGC                                      >  SRR4302547.772648/1‑94 (MQ=60)

                                                        GTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGC                                      >  SRR4302547.3190534/1‑94 (MQ=60)

                                                        GTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGC                                      <  SRR4302547.3270047/94‑1 (MQ=60)

                                                         TCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCC                                     >  SRR4302547.3438176/1‑94 (MQ=60)

                                                           GGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGT                                   <  SRR4302547.413103/94‑1 (MQ=60)

                                                            GCGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTC                                  <  SRR4302547.3698562/94‑1 (MQ=60)

                                                             CGAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCA                                 <  SRR4302547.675395/94‑1 (MQ=60)

                                                              GAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAT                                >  SRR4302547.3147388/1‑94 (MQ=60)

                                                              GAAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCATAAAGTGCGCCGTCAT                                >  SRR4302547.701092/1‑94 (MQ=60)

                                                               AAAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATC                               <  SRR4302547.5194242/94‑1 (MQ=60)

                                                                AAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCG                              >  SRR4302547.5034728/1‑94 (MQ=60)

                                                                AAATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCG                              <  SRR4302547.467503/94‑1 (MQ=60)

                                                                  ATTTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTG                            <  SRR4302547.5683549/94‑1 (MQ=60)

                                                                    TTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGAT                          >  SRR4302547.4230683/1‑94 (MQ=60)

                                                                    TTTCCCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGAT                          >  SRR4302547.266840/1‑94 (MQ=60)

                                                                       CCCCGACAGTAAAACATTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTT                       >  SRR4302547.5226899/1‑94 (MQ=60)

                                                                        CCCGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTG                      >  SRR4302547.6410195/1‑94 (MQ=60)

                                                                          CGACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGAC                    >  SRR4302547.1053355/1‑94 (MQ=60)

                                                                           GACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACT                   >  SRR4302547.2667711/1‑94 (MQ=60)

                                                                            ACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTT                  >  SRR4302547.6341101/1‑94 (MQ=60)

                                                                            ACAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTT                  >  SRR4302547.3668286/1‑94 (MQ=60)

                                                                             CAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTG                 <  SRR4302547.291727/94‑1 (MQ=60)

                                                                             CAGTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTG                 <  SRR4302547.5684254/94‑1 (MQ=60)

                                                                              AGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGC                <  SRR4302547.6405071/94‑1 (MQ=60)

                                                                               GTAAAACTTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGCAAAGTGCGCCGTCATCGTCAATTTGCCTTGCG               >  SRR4302547.4141818/1‑94 (MQ=60)

                                                                               GTAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCG               >  SRR4302547.4012708/1‑94 (MQ=60)

                                                                                TAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGC              >  SRR4302547.6665906/1‑94 (MQ=60)

                                                                                TAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGC              >  SRR4302547.1565706/1‑94 (MQ=60)

                                                                                TAAAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGC              >  SRR4302547.4589308/1‑94 (MQ=60)

                                                                                  AAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA            <  SRR4302547.2257387/94‑1 (MQ=60)

                                                                                  AAACCTTTCCCAACTTCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA            <  SRR4302547.4493225/94‑1 (MQ=60)

                                                                                  AAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA            >  SRR4302547.4320175/1‑94 (MQ=60)

                                                                                  AAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA            >  SRR4302547.4329442/1‑94 (MQ=60)

                                                                                  AAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA            >  SRR4302547.2302271/1‑94 (MQ=60)

                                                                                  AAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA            >  SRR4302547.4941718/1‑94 (MQ=60)

                                                                                  AAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGAGCGCCGTCATCGTGATTTTGACTTGCGCAA            >  SRR4302547.555136/1‑94 (MQ=60)

                                                                                  AAACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGAGCGCCGTCATCGCGATTTTGACTTGCGCAA            >  SRR4302547.604295/1‑94 (MQ=60)

                                                                                   ACCCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAG           <  SRR4302547.2682703/94‑1 (MQ=60)

                                                                                   AACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAG           >  SRR4302547.5966301/1‑94 (MQ=60)

                                                                                    ACCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGT          <  SRR4302547.941111/94‑1 (MQ=60)

                                                                                     CCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTT         >  SRR4302547.186551/1‑94 (MQ=60)

                                                                                     CCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTT         <  SRR4302547.6346713/94‑1 (MQ=60)

                                                                                     CCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTT         >  SRR4302547.5411545/1‑94 (MQ=60)

                                                                                     CCTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTT         >  SRR4302547.5133009/1‑94 (MQ=60)

                                                                                      CTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTT        >  SRR4302547.5401964/1‑94 (MQ=60)

                                                                                      CTTTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTT        >  SRR4302547.3157257/1‑94 (MQ=60)

                                                                                        TTCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGT      <  SRR4302547.6762976/94‑1 (MQ=60)

                                                                                         TCCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTT     <  SRR4302547.4074303/94‑1 (MQ=60)

                                                                                          CCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTG    >  SRR4302547.239883/1‑94 (MQ=60)

                                                                                          CCCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATCTTGACTTGCGCAAGTTTGTTG    <  SRR4302547.6639306/94‑1 (MQ=60)

                                                                                           CCAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGA   >  SRR4302547.2133738/1‑94 (MQ=60)

                                                                                            CAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAA  <  SRR4302547.4923183/94‑1 (MQ=60)

                                                                                            CAACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAA  >  SRR4302547.3419720/1‑94 (MQ=60)

GCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAA  >  NC_000913/3667366‑3667551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`