Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,782,932 T→C Y16H (TAT→CAT)  yibO → putative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9320TC100.0% 923.8 / NA 245Y16H (TAT→CAT) yibOputative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (129/116);  total (129/116)

Only 100 of 276 total aligned reads displayed.

AGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACC  >  NC_000913/3782842‑3783021
                                                                                          |                                                                                         
agtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccat                                                                                         >  2:1969480/1‑93 (MQ=255)
agtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccat                                                                                         <  2:342172/93‑1 (MQ=255)
agtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccat                                                                                         >  1:3414213/1‑93 (MQ=255)
 gtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccatg                                                                                        <  2:896808/93‑1 (MQ=255)
    aaaTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccatggct                                                                                     >  1:6284294/1‑93 (MQ=255)
     aaTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGAtggccatggcta                                                                                    >  2:3276165/1‑93 (MQ=255)
      aTTACGCAAATTTTGGCTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATggccatggctat                                                                                   <  1:5529963/93‑1 (MQ=255)
      aTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATggccatggctat                                                                                   <  2:5357300/93‑1 (MQ=255)
       ttACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATc                                                                                  <  2:5940371/93‑1 (MQ=255)
          cGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATcgcg                                                                               <  1:428431/93‑1 (MQ=255)
          cGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATcgcg                                                                               <  2:1470327/93‑1 (MQ=255)
               aTTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCgaagaa                                                                          <  1:6509641/93‑1 (MQ=255)
                ttttGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAc                                                                         <  1:1376482/93‑1 (MQ=255)
                 tttGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAca                                                                        <  2:2544486/93‑1 (MQ=255)
                     aCTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAcagcag                                                                    <  1:171172/93‑1 (MQ=255)
                      ctctTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAgg                                                                   >  2:6461130/1‑93 (MQ=255)
                       tctTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACTTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGa                                                                  <  1:5339959/93‑1 (MQ=255)
                       tctTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGa                                                                  <  1:3777819/93‑1 (MQ=255)
                        ctTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAt                                                                 <  2:1020454/93‑1 (MQ=255)
                        ctTGAGTATGAGGTTGTCGCAATGATGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAt                                                                 <  2:1305356/93‑1 (MQ=255)
                         ttGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATa                                                                >  2:4770315/1‑93 (MQ=255)
                         ttGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATa                                                                <  2:4589112/93‑1 (MQ=255)
                         ttGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATa                                                                <  2:76754/93‑1 (MQ=255)
                            aGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACg                                                             >  2:5164163/1‑93 (MQ=255)
                             gTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAGCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGc                                                            <  1:5683489/93‑1 (MQ=255)
                             gTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGc                                                            <  1:3195720/93‑1 (MQ=255)
                              tATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGcc                                                           <  2:3878123/93‑1 (MQ=255)
                              tATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGcc                                                           <  2:5638810/93‑1 (MQ=255)
                              tATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGcc                                                           >  2:1380880/1‑93 (MQ=255)
                              tATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTCATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGcc                                                           <  1:396107/93‑1 (MQ=255)
                               aTGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCa                                                          >  1:5209706/1‑93 (MQ=255)
                                tGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAt                                                         >  1:977325/1‑93 (MQ=255)
                                 gAGGTTGTCGCAATGTTGGTTTCTAAAAATCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAtt                                                        <  1:3449924/93‑1 (MQ=255)
                                  aGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAttt                                                       >  1:739507/1‑93 (MQ=255)
                                   ggTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAtttt                                                      >  2:4109485/1‑93 (MQ=255)
                                   ggTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAtttt                                                      >  1:1919523/1‑93 (MQ=255)
                                   ggTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAtttt                                                      >  2:4200839/1‑93 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    >  2:4296408/1‑93 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    <  2:4315865/93‑1 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    >  2:4862193/1‑93 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    <  2:4967040/93‑1 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    <  1:3855860/93‑1 (MQ=255)
                                     ttGTCGCAATGATGGGTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCCTTTCTa                                                    >  2:6215282/1‑93 (MQ=255)
                                     tcgTCGCAATGTTGGTTTCTAAAAACCCTATGGTACTGGTGATTCTGAATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    <  1:2795061/91‑1 (MQ=255)
                                      tGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAg                                                   <  1:2924223/93‑1 (MQ=255)
                                        tCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTg                                                 >  1:4701913/1‑93 (MQ=255)
                                          gCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCt                                               >  1:5021085/1‑93 (MQ=255)
                                           cAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTa                                              >  1:3670507/1‑93 (MQ=255)
                                           cAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTa                                              <  2:2765491/93‑1 (MQ=255)
                                            aaTGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTaa                                             >  2:3929806/1‑93 (MQ=255)
                                             aTGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTaaa                                            >  1:2290234/1‑93 (MQ=255)
                                              tGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTaaaa                                           >  2:1890012/1‑93 (MQ=255)
                                               gTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAc                                          >  1:5775686/1‑93 (MQ=255)
                                                ttGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAcc                                         >  1:4221522/1‑93 (MQ=255)
                                                 tGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAccc                                        <  2:5996642/93‑1 (MQ=255)
                                                  ggTTTCTAAAAATCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAcccc                                       <  2:1104193/93‑1 (MQ=255)
                                                  ggTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAcccc                                       <  2:135719/93‑1 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      <  2:1717611/93‑1 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      <  1:2257745/93‑1 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      >  1:5965733/1‑93 (MQ=255)
                                                    tttCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCgg                                     >  2:23045/1‑93 (MQ=255)
                                                     ttCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGt                                    >  2:6516349/1‑93 (MQ=255)
                                                     ttCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGt                                    <  1:6376458/93‑1 (MQ=255)
                                                      tCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTa                                   <  1:5125710/93‑1 (MQ=255)
                                                      tCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTa                                   <  2:366340/93‑1 (MQ=255)
                                                        tAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAt                                 <  2:1601862/93‑1 (MQ=255)
                                                        tAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAt                                 >  2:5022132/1‑93 (MQ=255)
                                                         aaaaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATg                                <  2:4821286/93‑1 (MQ=255)
                                                          aaaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATgg                               >  2:2337536/1‑93 (MQ=255)
                                                          aaaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATgg                               >  2:6610479/1‑93 (MQ=255)
                                                          aaaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATgg                               >  1:513555/1‑93 (MQ=255)
                                                           aaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGa                              <  1:1796716/93‑1 (MQ=255)
                                                           aaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGa                              <  2:3583777/93‑1 (MQ=255)
                                                           aaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGa                              >  2:5892529/1‑93 (MQ=255)
                                                           aaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGa                              >  2:3157188/1‑93 (MQ=255)
                                                            aaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAt                             <  2:4064901/93‑1 (MQ=255)
                                                             aaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATg                            <  1:6047622/93‑1 (MQ=255)
                                                             aaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATg                            >  1:2611553/1‑93 (MQ=255)
                                                             aaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATg                            >  1:1136924/1‑93 (MQ=255)
                                                                cTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAc                         >  2:3019122/1‑93 (MQ=255)
                                                                 tATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACt                        >  1:2953845/1‑93 (MQ=255)
                                                                    ggTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACtgtg                     <  1:4321501/93‑1 (MQ=255)
                                                                    ggTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACtgtg                     <  1:964567/93‑1 (MQ=255)
                                                                        cTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGcc                 >  2:5324655/1‑93 (MQ=255)
                                                                         tGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCa                >  1:3500805/1‑93 (MQ=255)
                                                                         tGGTCATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTCAGCCa                >  2:3042797/1‑93 (MQ=255)
                                                                          ggTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACACCGGTAATGGATGCACTGTGGGACaa               >  2:3373962/1‑93 (MQ=255)
                                                                          ggTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAGAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCaa               >  2:4673519/1‑93 (MQ=255)
                                                                            tGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATc             >  1:2001332/1‑93 (MQ=255)
                                                                            tGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATc             <  2:6425473/93‑1 (MQ=255)
                                                                             gATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCg            <  1:4572308/93‑1 (MQ=255)
                                                                               ttCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTc          <  1:2532771/93‑1 (MQ=255)
                                                                                 cTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCg        <  1:5171082/93‑1 (MQ=255)
                                                                                  tGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGc       >  1:4514405/1‑93 (MQ=255)
                                                                                   ggATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCa      >  2:3056082/1‑93 (MQ=255)
                                                                                   ggATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCa      >  1:729157/1‑93 (MQ=255)
                                                                                   ggATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCa      <  1:62573/93‑1 (MQ=255)
                                                                                    gATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAt     >  2:5265297/1‑93 (MQ=255)
                                                                                     atggccatggctATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATa    <  1:1201249/93‑1 (MQ=255)
                                                                                     atggccatggctATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATa    >  2:4423448/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACC  >  NC_000913/3782842‑3783021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  NC_000913/3782839‑3783025
                                                                                             |                                                                                             
TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCC                                                                                               >  SRR4302547.975257/1‑94 (MQ=60)
TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCC                                                                                               >  SRR4302547.6310426/1‑94 (MQ=60)
 GTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCA                                                                                              >  SRR4302547.1783636/1‑94 (MQ=60)
  TAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCAT                                                                                             >  SRR4302547.3483736/1‑94 (MQ=60)
  TAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCAT                                                                                             <  SRR4302547.3443958/94‑1 (MQ=60)
  TAGTAAAATTACGCAAATTTTGACTCTTGAGTACGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCAT                                                                                             >  SRR4302547.2369047/1‑94 (MQ=60)
   AGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATG                                                                                            <  SRR4302547.882249/94‑1 (MQ=60)
     TAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGC                                                                                          <  SRR4302547.3887265/94‑1 (MQ=60)
      AAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCT                                                                                         >  SRR4302547.6414858/1‑94 (MQ=60)
       AAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTA                                                                                        >  SRR4302547.5293126/1‑94 (MQ=60)
       AAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTA                                                                                        <  SRR4302547.4399746/94‑1 (MQ=60)
        AATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTAT                                                                                       >  SRR4302547.3020916/1‑94 (MQ=60)
         ATTACGCAAATTTTGGCTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      <  SRR4302547.5644106/94‑1 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      >  SRR4302547.5726214/1‑94 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      <  SRR4302547.5126435/94‑1 (MQ=60)
            ACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCG                                                                                   <  SRR4302547.24076/94‑1 (MQ=60)
            ACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCG                                                                                   >  SRR4302547.1359392/1‑94 (MQ=60)
             CGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGA                                                                                  <  SRR4302547.436365/94‑1 (MQ=60)
               CAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAG                                                                                >  SRR4302547.805385/1‑94 (MQ=60)
                  ATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAC                                                                             <  SRR4302547.6645172/94‑1 (MQ=60)
                  ATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAC                                                                             <  SRR4302547.5271077/94‑1 (MQ=60)
                   TTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACA                                                                            >  SRR4302547.5659545/1‑94 (MQ=60)
                   TTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACA                                                                            <  SRR4302547.986573/94‑1 (MQ=60)
                   TTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACA                                                                            <  SRR4302547.1404076/94‑1 (MQ=60)
                    TTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATTGCCATGGCTATCGCGAAGAACAG                                                                           >  SRR4302547.201040/1‑94 (MQ=60)
                    TTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAG                                                                           >  SRR4302547.1840601/1‑94 (MQ=60)
                    TTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAG                                                                           >  SRR4302547.292623/1‑94 (MQ=60)
                     TTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCCATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGC                                                                          <  SRR4302547.747504/94‑1 (MQ=60)
                      TGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCA                                                                         <  SRR4302547.2699359/94‑1 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       <  SRR4302547.6475875/94‑1 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       <  SRR4302547.174307/94‑1 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       >  SRR4302547.6250732/1‑94 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       <  SRR4302547.5393890/94‑1 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       >  SRR4302547.2271463/1‑94 (MQ=60)
                          TCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACTTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAT                                                                     <  SRR4302547.5450026/94‑1 (MQ=60)
                          TCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAT                                                                     <  SRR4302547.3870532/94‑1 (MQ=60)
                          TCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAT                                                                     <  SRR4302547.3854859/94‑1 (MQ=60)
                           CTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATA                                                                    <  SRR4302547.266031/94‑1 (MQ=60)
                           CTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATA                                                                    >  SRR4302547.5476659/1‑94 (MQ=60)
                            TTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAA                                                                   >  SRR4302547.5274600/1‑94 (MQ=60)
                              GAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACG                                                                 >  SRR4302547.3758142/1‑94 (MQ=60)
                              GAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACG                                                                 <  SRR4302547.5234203/94‑1 (MQ=60)
                               AGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGC                                                                >  SRR4302547.6135291/1‑94 (MQ=60)
                                GTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAGCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCC                                                               <  SRR4302547.5800839/94‑1 (MQ=60)
                                GTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCC                                                               <  SRR4302547.3259407/94‑1 (MQ=60)
                                 TATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCA                                                              <  SRR4302547.178049/94‑1 (MQ=60)
                                 TATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCA                                                              >  SRR4302547.5316917/1‑94 (MQ=60)
                                 TATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCA                                                              <  SRR4302547.2317978/94‑1 (MQ=60)
                                 TATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTCATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCA                                                              <  SRR4302547.403471/94‑1 (MQ=60)
                                  ATGAGGTTGTCGCAATGTTGGTTTCTCAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGACAAGCAGGATCACGCCGT                                                             >  SRR4302547.5955106/1‑94 (MQ=60)
                                  ATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAT                                                             >  SRR4302547.996331/1‑94 (MQ=60)
                                   TGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATT                                                            >  SRR4302547.4538382/1‑94 (MQ=60)
                                    GAGGTTGTCGCAATGTTGGTTTCTAAAAATCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTT                                                           <  SRR4302547.3520246/94‑1 (MQ=60)
                                    GAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTT                                                           >  SRR4302547.753371/1‑94 (MQ=60)
                                     AGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTT                                                          >  SRR4302547.5991652/1‑94 (MQ=60)
                                     AGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTT                                                          >  SRR4302547.1957540/1‑94 (MQ=60)
                                      GGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTT                                                         >  SRR4302547.6675897/1‑94 (MQ=60)
                                       GTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTA                                                        <  SRR4302547.2314948/94‑1 (MQ=60)
                                        TTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAG                                                       <  SRR4302547.3934343/94‑1 (MQ=60)
                                        TCGTCGCAATGTTGGTTTCTAAAAACCCTATGGTACTGGTGATTCTGAATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAG                                                       <  SRR4302547.2851016/94‑1 (MQ=60)
                                         TGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACTCCATTTTTAGT                                                      <  SRR4302547.1482243/94‑1 (MQ=60)
                                         TGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGT                                                      <  SRR4302547.2982532/94‑1 (MQ=60)
                                          GTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTG                                                     >  SRR4302547.4799295/1‑94 (MQ=60)
                                          GTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTG                                                     >  SRR4302547.3739047/1‑94 (MQ=60)
                                            CGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCT                                                   >  SRR4302547.5124841/1‑94 (MQ=60)
                                             GCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTA                                                  >  SRR4302547.4708883/1‑94 (MQ=60)
                                             GCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTA                                                  <  SRR4302547.3475484/94‑1 (MQ=60)
                                             GCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTA                                                  >  SRR4302547.3745370/1‑94 (MQ=60)
                                             GCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTA                                                  <  SRR4302547.3514928/94‑1 (MQ=60)
                                              CAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAA                                                 >  SRR4302547.4000789/1‑94 (MQ=60)
                                               AATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAA                                                >  SRR4302547.2336630/1‑94 (MQ=60)
                                               AATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAA                                                >  SRR4302547.1179612/1‑94 (MQ=60)
                                               AATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAA                                                >  SRR4302547.5822453/1‑94 (MQ=60)
                                                ATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAA                                               <  SRR4302547.638461/94‑1 (MQ=60)
                                                 TGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAC                                              >  SRR4302547.5941283/1‑94 (MQ=60)
                                                 TGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAC                                              >  SRR4302547.5895147/1‑94 (MQ=60)
                                                  GTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACC                                             >  SRR4302547.4307743/1‑94 (MQ=60)
                                                   TTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCC                                            <  SRR4302547.3752424/94‑1 (MQ=60)
                                                    TGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCC                                           <  SRR4302547.5858807/94‑1 (MQ=60)
                                                     GGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCG                                          >  SRR4302547.6089460/1‑94 (MQ=60)
                                                      GTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGG                                         >  SRR4302547.4937303/1‑94 (MQ=60)
                                                      GTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGG                                         <  SRR4302547.2303341/94‑1 (MQ=60)
                                                       TTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGT                                        <  SRR4302547.6456822/94‑1 (MQ=60)
                                                        TTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTA                                       <  SRR4302547.6509511/94‑1 (MQ=60)
                                                         TCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAA                                      <  SRR4302547.5231328/94‑1 (MQ=60)
                                                          CTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAT                                     >  SRR4302547.4368210/1‑94 (MQ=60)
                                                            AAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGG                                   >  SRR4302547.522880/1‑94 (MQ=60)
                                                             AAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGA                                  >  SRR4302547.4286372/1‑94 (MQ=60)
                                                              AAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAT                                 <  SRR4302547.1832261/94‑1 (MQ=60)
                                                              AAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAT                                 <  SRR4302547.744295/94‑1 (MQ=60)
                                                              AAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAT                                 <  SRR4302547.1511585/94‑1 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                >  SRR4302547.2664176/1‑94 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                <  SRR4302547.3546182/94‑1 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                >  SRR4302547.1159885/1‑94 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                <  SRR4302547.4357454/94‑1 (MQ=60)
                                                                AACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGC                               <  SRR4302547.6173096/94‑1 (MQ=60)
                                                                 ACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCA                              <  SRR4302547.5301013/94‑1 (MQ=60)
                                                                  CCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAC                             >  SRR4302547.46006/1‑94 (MQ=60)
                                                                  CCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAC                             >  SRR4302547.6705571/1‑94 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            >  SRR4302547.3012644/1‑94 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            >  SRR4302547.5339658/1‑94 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            >  SRR4302547.4397799/1‑94 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            <  SRR4302547.4578003/94‑1 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGAATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            >  SRR4302547.6550238/1‑94 (MQ=60)
                                                                    TATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTG                           <  SRR4302547.3510257/94‑1 (MQ=60)
                                                                      TGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTG                         >  SRR4302547.275470/1‑94 (MQ=60)
                                                                      TGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTG                         >  SRR4302547.199830/1‑94 (MQ=60)
                                                                      TGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTG                         <  SRR4302547.3584381/94‑1 (MQ=60)
                                                                       GGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGG                        <  SRR4302547.983300/94‑1 (MQ=60)
                                                                       GGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGG                        <  SRR4302547.4410461/94‑1 (MQ=60)
                                                                        GTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGG                       <  SRR4302547.3466164/94‑1 (MQ=60)
                                                                         TACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGC                      <  SRR4302547.294202/94‑1 (MQ=60)
                                                                           CTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCA                    >  SRR4302547.3572247/1‑94 (MQ=60)
                                                                            TGGTGATTCTGGATGGCCATGGCTGTCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAA                   <  SRR4302547.4137945/94‑1 (MQ=60)
                                                                             GGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAT                  >  SRR4302547.1793087/1‑94 (MQ=60)
                                                                              GTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATC                 >  SRR4302547.2041159/1‑94 (MQ=60)
                                                                               TGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCG                >  SRR4302547.5167535/1‑94 (MQ=60)
                                                                                GATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGT               <  SRR4302547.4666922/94‑1 (MQ=60)
                                                                                 ATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTC              <  SRR4302547.4678131/94‑1 (MQ=60)
                                                                                  TTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCC             >  SRR4302547.6375081/1‑94 (MQ=60)
                                                                                  TTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCC             <  SRR4302547.2583964/94‑1 (MQ=60)
                                                                                    CTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGC           >  SRR4302547.4607677/1‑94 (MQ=60)
                                                                                    CTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGC           <  SRR4302547.5277542/94‑1 (MQ=60)
                                                                                     TGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCA          >  SRR4302547.742757/1‑94 (MQ=60)
                                                                                     TGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCA          >  SRR4302547.299670/1‑94 (MQ=60)
                                                                                     TGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCA          >  SRR4302547.138910/1‑94 (MQ=60)
                                                                                      GGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAT         <  SRR4302547.63749/94‑1 (MQ=60)
                                                                                      GGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAT         <  SRR4302547.2654089/94‑1 (MQ=60)
                                                                                       GATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATA        <  SRR4302547.4807879/94‑1 (MQ=60)
                                                                                        ATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAC       <  SRR4302547.6408526/94‑1 (MQ=60)
                                                                                        ATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAC       >  SRR4302547.310136/1‑94 (MQ=60)
                                                                                        ATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAC       >  SRR4302547.2187803/1‑94 (MQ=60)
                                                                                        ATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAC       <  SRR4302547.1225497/94‑1 (MQ=60)
                                                                                           GCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCT    <  SRR4302547.2030880/94‑1 (MQ=60)
                                                                                            CCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGTCAATCGTCCGCATACCCTA   >  SRR4302547.674682/1‑94 (MQ=60)
                                                                                            CCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTA   >  SRR4302547.1816054/1‑94 (MQ=60)
                                                                                             CATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  SRR4302547.2494743/1‑94 (MQ=60)
                                                                                             CATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  SRR4302547.3542299/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  NC_000913/3782839‑3783025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑