Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,782,932 T→C Y16H (TAT→CAT)  yibO → putative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9320TC99.5% 796.3 / ‑4.8 217Y16H (TAT→CAT) yibOputative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (118/98);  total (119/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 250 total aligned reads displayed.

AGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACC  >  NC_000913/3782842‑3783021
                                                                                          |                                                                                         
agtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccat                                                                                         >  2:5546503/1‑93 (MQ=255)
 gtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccatg                                                                                        >  2:1803598/1‑93 (MQ=255)
 gtaAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccatg                                                                                        <  2:4240425/93‑1 (MQ=255)
 gtaAAATTACACAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccatg                                                                                        >  1:3811292/1‑93 (MQ=255)
    aaaTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGatggccatggct                                                                                     <  2:2559630/93‑1 (MQ=255)
     aaTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGAtggccatggcta                                                                                    <  1:5024337/93‑1 (MQ=255)
     aaTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGAtggccatggcta                                                                                    <  1:1001967/93‑1 (MQ=255)
      aTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATggccatggctat                                                                                   <  2:3945770/93‑1 (MQ=255)
      aTTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATggccatggctat                                                                                   <  1:244265/93‑1 (MQ=255)
      aTTACCGAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATggccatggctat                                                                                   <  2:1928602/93‑1 (MQ=255)
       ttACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATc                                                                                  >  1:4010991/1‑93 (MQ=255)
       ttACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATc                                                                                  >  2:3267476/1‑93 (MQ=255)
          cGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATcgcg                                                                               >  1:4594986/1‑93 (MQ=255)
          cGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATcgcg                                                                               <  1:3750699/93‑1 (MQ=255)
          cGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATcgcg                                                                               <  1:1120503/93‑1 (MQ=255)
            cAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCgaa                                                                             >  1:1324133/1‑93 (MQ=255)
            cAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCgaa                                                                             <  1:5040649/93‑1 (MQ=255)
               aTTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCgaagaa                                                                          <  1:5142578/93‑1 (MQ=255)
                ttttGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAc                                                                         <  2:1533841/93‑1 (MQ=255)
                ttttGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAc                                                                         <  1:2903215/93‑1 (MQ=255)
                ttttGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAc                                                                         >  1:999621/1‑93 (MQ=255)
                  ttGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAcag                                                                       <  2:4613807/93‑1 (MQ=255)
                    gACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAccgca                                                                     <  1:6020230/93‑1 (MQ=255)
                    gACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAcagca                                                                     <  1:2280479/93‑1 (MQ=255)
                    gACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAcagca                                                                     <  2:1491245/93‑1 (MQ=255)
                     aCTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAcagcag                                                                    <  2:321469/93‑1 (MQ=255)
                     aCTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAcagcag                                                                    >  1:1186746/1‑93 (MQ=255)
                      ctctTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAgg                                                                   >  1:474752/1‑93 (MQ=255)
                          tGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATaa                                                               <  1:319950/93‑1 (MQ=255)
                          tGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATaa                                                               <  2:2078060/93‑1 (MQ=255)
                          tGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATaa                                                               <  1:295766/93‑1 (MQ=255)
                          tGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATaa                                                               <  2:2641104/93‑1 (MQ=255)
                           gAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAc                                                              >  2:4055152/1‑93 (MQ=255)
                           gAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAc                                                              >  1:2789226/1‑93 (MQ=255)
                             gTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGc                                                            >  2:436652/1‑93 (MQ=255)
                               aTGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCa                                                          >  2:6131410/1‑93 (MQ=255)
                               aTGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCa                                                          <  2:3112722/93‑1 (MQ=255)
                                tGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAt                                                         >  1:721591/1‑93 (MQ=255)
                                   ggTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAtttt                                                      <  2:5925680/93‑1 (MQ=255)
                                    gTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAttttt                                                     <  2:5454327/93‑1 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAGCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    <  1:1465452/93‑1 (MQ=255)
                                     ttGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTa                                                    >  1:21433/1‑93 (MQ=255)
                                      tGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAg                                                   <  1:1247792/93‑1 (MQ=255)
                                      tGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAg                                                   <  2:5631544/93‑1 (MQ=255)
                                        tCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTg                                                 <  2:3546076/93‑1 (MQ=255)
                                          gCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCt                                               >  2:1066377/1‑93 (MQ=255)
                                            aaTGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTaa                                             >  1:3136532/1‑93 (MQ=255)
                                            aaTGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTaa                                             <  1:2991131/93‑1 (MQ=255)
                                              tGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTaaaa                                           <  1:4042254/93‑1 (MQ=255)
                                              tGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAAAACAGGAGAACGCCATTTTTTTTTCTaaac                                           >  2:1271903/1‑92 (MQ=255)
                                               gTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAc                                          >  1:4940587/1‑93 (MQ=255)
                                                ttGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAcc                                         >  1:3244518/1‑93 (MQ=255)
                                                  ggTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAcccc                                       >  2:4564750/1‑93 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      >  2:3351483/1‑93 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      >  1:258409/1‑93 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      >  2:3689909/1‑93 (MQ=255)
                                                   gTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCg                                      <  2:2788369/93‑1 (MQ=255)
                                                    tttCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCgg                                     >  1:1485959/1‑93 (MQ=255)
                                                     ttCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGt                                    <  2:3624136/93‑1 (MQ=255)
                                                       cTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTaa                                  <  2:5956518/93‑1 (MQ=255)
                                                       cTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTaa                                  <  1:4430672/93‑1 (MQ=255)
                                                       cTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTaa                                  <  2:3359930/93‑1 (MQ=255)
                                                       cTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTaa                                  <  1:4250904/93‑1 (MQ=255)
                                                        tAAAAAACCTATGGTACTGGGGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAt                                 >  2:2382874/1‑93 (MQ=255)
                                                         aaaaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATg                                >  2:5854510/1‑93 (MQ=255)
                                                          aaaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATgg                               <  1:5705939/93‑1 (MQ=255)
                                                          aaaaaCCTATGGGCCTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATgg                               >  1:1501074/1‑93 (MQ=255)
                                                           aaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGa                              <  1:745132/93‑1 (MQ=255)
                                                           aaaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGa                              <  1:1979101/93‑1 (MQ=255)
                                                            aaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAt                             >  2:4695027/1‑93 (MQ=255)
                                                            aaaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAt                             <  1:1373683/93‑1 (MQ=255)
                                                             aaCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATg                            >  1:5387355/1‑93 (MQ=255)
                                                               ccTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCa                          >  2:3837226/1‑93 (MQ=255)
                                                                cTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAc                         >  1:144410/1‑93 (MQ=255)
                                                                cTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAc                         <  2:5685865/93‑1 (MQ=255)
                                                                 tATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACt                        >  1:3859066/1‑93 (MQ=255)
                                                                  aTGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACtg                       >  1:1275286/1‑93 (MQ=255)
                                                                   tGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACtgt                      >  1:6059452/1‑93 (MQ=255)
                                                                     gTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTgg                    <  1:3626020/93‑1 (MQ=255)
                                                                      tACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTggg                   <  2:3030139/93‑1 (MQ=255)
                                                                      tACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTggg                   >  2:2008994/1‑93 (MQ=255)
                                                                        cTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGcc                 >  1:3519041/1‑93 (MQ=255)
                                                                        cTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGcc                 >  1:896621/1‑93 (MQ=255)
                                                                         tGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGGTGCACTGTGGGCCa                >  1:4144788/1‑93 (MQ=255)
                                                                         tGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCa                >  1:3032718/1‑93 (MQ=255)
                                                                          ggTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCaa               <  2:5274678/93‑1 (MQ=255)
                                                                           gTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAt              >  1:2716227/1‑93 (MQ=255)
                                                                           gTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAt              >  1:4646338/1‑93 (MQ=255)
                                                                             gATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCg            >  1:3970671/1‑93 (MQ=255)
                                                                             gATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCg            >  1:5557811/1‑93 (MQ=255)
                                                                               ttCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTc          >  1:4527396/1‑93 (MQ=255)
                                                                               ttCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTc          <  2:4741378/93‑1 (MQ=255)
                                                                                tCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTcc         <  1:2293999/93‑1 (MQ=255)
                                                                                tCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAAGCACTGTGGGCCAATCGTcc         <  1:5866578/93‑1 (MQ=255)
                                                                                 cTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCg        <  1:2914464/93‑1 (MQ=255)
                                                                                 cTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCg        >  2:5151782/1‑93 (MQ=255)
                                                                                  tGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGc       >  1:679200/1‑93 (MQ=255)
                                                                                   ggATGGCCATGGCTATCGCGAAGAACAGCAGGAAAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCa      >  1:5237317/1‑93 (MQ=255)
                                                                                    gATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAt     >  1:106572/1‑93 (MQ=255)
                                                                                      tggccatggctaTCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAc   >  1:613807/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACC  >  NC_000913/3782842‑3783021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  NC_000913/3782839‑3783025
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TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCC                                                                                               >  SRR4302532.5417362/1‑94 (MQ=60)
 GTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCA                                                                                              <  SRR4302532.2157693/94‑1 (MQ=60)
 GTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCA                                                                                              <  SRR4302532.2157692/94‑1 (MQ=60)
 GTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCA                                                                                              >  SRR4302532.3201600/1‑94 (MQ=60)
 GTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCA                                                                                              >  SRR4302532.4013593/1‑94 (MQ=60)
  TAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCAT                                                                                             >  SRR4302532.788287/1‑94 (MQ=60)
  TAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCAT                                                                                             <  SRR4302532.185571/94‑1 (MQ=60)
  TAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCAT                                                                                             >  SRR4302532.1770478/1‑94 (MQ=60)
   AGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATG                                                                                            >  SRR4302532.4882968/1‑94 (MQ=60)
   AGTAAAATTACACAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATG                                                                                            >  SRR4302532.3939972/1‑94 (MQ=60)
      AAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCT                                                                                         <  SRR4302532.2249667/94‑1 (MQ=60)
      AAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCT                                                                                         >  SRR4302532.1311793/1‑94 (MQ=60)
      AAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCT                                                                                         <  SRR4302532.5202356/94‑1 (MQ=60)
       AAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTA                                                                                        <  SRR4302532.4559007/94‑1 (MQ=60)
        AATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTAT                                                                                       >  SRR4302532.3538132/1‑94 (MQ=60)
        AATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTAT                                                                                       <  SRR4302532.1036076/94‑1 (MQ=60)
        AATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTAT                                                                                       <  SRR4302532.5196657/94‑1 (MQ=60)
         ATTCCGCACATTTTGACTCTTGAGGATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      <  SRR4302532.730183/94‑1 (MQ=60)
         ATTACGCAATTTTTGACTCTTGAGTATGAGGGTGTCGCAATGTTGGTTTCTAAAAATCCTATGGTACTGGTGATTCGGGATGGTCATGGCTTTC                                                                                      <  SRR4302532.730184/94‑1 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGTATGGCCATGGCTATC                                                                                      <  SRR4302532.247350/94‑1 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      <  SRR4302532.4774950/94‑1 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      >  SRR4302532.4264291/1‑94 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      >  SRR4302532.4146146/1‑94 (MQ=60)
         ATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATC                                                                                      <  SRR4302532.252717/94‑1 (MQ=60)
          TTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCG                                                                                     >  SRR4302532.2515381/1‑94 (MQ=60)
            ACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCG                                                                                   >  SRR4302532.4751632/1‑94 (MQ=60)
            ACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCG                                                                                   <  SRR4302532.5416840/94‑1 (MQ=60)
             CGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATTGCGA                                                                                  >  SRR4302532.4984751/1‑94 (MQ=60)
             CGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGA                                                                                  <  SRR4302532.1157918/94‑1 (MQ=60)
             CGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGA                                                                                  <  SRR4302532.3876796/94‑1 (MQ=60)
             CGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGA                                                                                  <  SRR4302532.1157919/94‑1 (MQ=60)
              GCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAA                                                                                 >  SRR4302532.1368378/1‑94 (MQ=60)
               CAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAG                                                                                <  SRR4302532.5213562/94‑1 (MQ=60)
                AAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGA                                                                               >  SRR4302532.2250841/1‑94 (MQ=60)
                 AATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGACGAA                                                                              >  SRR4302532.4392419/1‑94 (MQ=60)
                 AATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAA                                                                              >  SRR4302532.2885520/1‑94 (MQ=60)
                 AATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAA                                                                              >  SRR4302532.2360307/1‑94 (MQ=60)
                  ATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAC                                                                             >  SRR4302532.3439488/1‑94 (MQ=60)
                  ATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAC                                                                             <  SRR4302532.5319493/94‑1 (MQ=60)
                  ATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAAC                                                                             >  SRR4302532.1033667/1‑94 (MQ=60)
                   TTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACA                                                                            <  SRR4302532.5889208/94‑1 (MQ=60)
                   TTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACA                                                                            <  SRR4302532.3010494/94‑1 (MQ=60)
                   TTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACA                                                                            <  SRR4302532.3000075/94‑1 (MQ=60)
                      TGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCA                                                                         <  SRR4302532.3032232/94‑1 (MQ=60)
                      TGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCA                                                                         <  SRR4302532.5760595/94‑1 (MQ=60)
                      TGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAACA                                                                         >  SRR4302532.1025754/1‑94 (MQ=60)
                       GACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACCGCAG                                                                        <  SRR4302532.6228129/94‑1 (MQ=60)
                       GACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAG                                                                        <  SRR4302532.2356176/94‑1 (MQ=60)
                       GACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAG                                                                        <  SRR4302532.4825287/94‑1 (MQ=60)
                       GACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAG                                                                        >  SRR4302532.1226415/1‑94 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       >  SRR4302532.491295/1‑94 (MQ=60)
                        ACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGG                                                                       <  SRR4302532.679572/94‑1 (MQ=60)
                         CTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGA                                                                      >  SRR4302532.4157774/1‑94 (MQ=60)
                          TCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAT                                                                     >  SRR4302532.3097855/1‑94 (MQ=60)
                          TCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGAT                                                                     <  SRR4302532.1483734/94‑1 (MQ=60)
                           CTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATA                                                                    <  SRR4302532.6158848/94‑1 (MQ=60)
                            TTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAA                                                                   <  SRR4302532.770691/94‑1 (MQ=60)
                             TGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAC                                                                  >  SRR4302532.2882754/1‑94 (MQ=60)
                             TGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAC                                                                  <  SRR4302532.331062/94‑1 (MQ=60)
                             TGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAC                                                                  <  SRR4302532.331061/94‑1 (MQ=60)
                             TGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAC                                                                  <  SRR4302532.305969/94‑1 (MQ=60)
                             TGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAAC                                                                  <  SRR4302532.6236065/94‑1 (MQ=60)
                               AGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGC                                                                <  SRR4302532.3085839/94‑1 (MQ=60)
                                 TATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACTGCAGGATAACGCCT                                                              >  SRR4302532.1344241/1‑94 (MQ=60)
                                  ATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAT                                                             >  SRR4302532.4638824/1‑94 (MQ=60)
                                  ATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAT                                                             >  SRR4302532.746214/1‑94 (MQ=60)
                                  ATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCAT                                                             >  SRR4302532.1406267/1‑94 (MQ=60)
                                   TGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATT                                                            >  SRR4302532.4543919/1‑94 (MQ=60)
                                      GGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTT                                                         <  SRR4302532.5116330/94‑1 (MQ=60)
                                       GTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTA                                                        >  SRR4302532.22294/1‑94 (MQ=60)
                                        TTGTCGCAATGTTGGTTTCTAAAAAGCCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAG                                                       <  SRR4302532.1514822/94‑1 (MQ=60)
                                        TTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAG                                                       <  SRR4302532.3956574/94‑1 (MQ=60)
                                         TGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGT                                                      <  SRR4302532.1289537/94‑1 (MQ=60)
                                          GTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTG                                                     >  SRR4302532.4259838/1‑94 (MQ=60)
                                          GTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTG                                                     <  SRR4302532.1446208/94‑1 (MQ=60)
                                           TCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGC                                                    <  SRR4302532.1117405/94‑1 (MQ=60)
                                            CGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCT                                                   >  SRR4302532.5235040/1‑94 (MQ=60)
                                              CAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAA                                                 >  SRR4302532.3682273/1‑94 (MQ=60)
                                              CAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAA                                                 >  SRR4302532.1108435/1‑94 (MQ=60)
                                              CAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAA                                                 >  SRR4302532.3240641/1‑94 (MQ=60)
                                              CAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAA                                                 >  SRR4302532.3199213/1‑94 (MQ=60)
                                               AATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAA                                                <  SRR4302532.3089978/94‑1 (MQ=60)
                                                 TTTTGGTTTCTAAAAAACCTATGGTATTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACTCCATTTTTAGTGCTAAAAC                                              >  SRR4302532.5682032/1‑94 (MQ=60)
                                                 TGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAC                                              <  SRR4302532.6294389/94‑1 (MQ=60)
                                                 TGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAC                                              <  SRR4302532.4178390/94‑1 (MQ=60)
                                                 TGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAC                                              >  SRR4302532.5110172/1‑94 (MQ=60)
                                                 TGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAAC                                              >  SRR4302532.4145321/1‑94 (MQ=60)
                                                  GTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACC                                             >  SRR4302532.3352240/1‑94 (MQ=60)
                                                   TTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCC                                            <  SRR4302532.2743307/94‑1 (MQ=60)
                                                   TTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCC                                            >  SRR4302532.3878840/1‑94 (MQ=60)
                                                   TTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCC                                            >  SRR4302532.2880161/1‑94 (MQ=60)
                                                     GGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCG                                          >  SRR4302532.1536632/1‑94 (MQ=60)
                                                     GGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCG                                          >  SRR4302532.267373/1‑94 (MQ=60)
                                                      GTTTCTAAAAAACCTATGGTACTTGTGAATCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGG                                         <  SRR4302532.6344556/94‑1 (MQ=60)
                                                      GTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGG                                         >  SRR4302532.1536083/1‑94 (MQ=60)
                                                       TTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGT                                        <  SRR4302532.1331987/94‑1 (MQ=60)
                                                       TTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGT                                        <  SRR4302532.1057164/94‑1 (MQ=60)
                                                          CTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAT                                     <  SRR4302532.1597907/94‑1 (MQ=60)
                                                          CTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAT                                     <  SRR4302532.4581630/94‑1 (MQ=60)
                                                          CTAAAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAAT                                     <  SRR4302532.4393749/94‑1 (MQ=60)
                                                            AAAAAACCTATGGGCCTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGG                                   >  SRR4302532.1551726/1‑94 (MQ=60)
                                                             AAAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGA                                  <  SRR4302532.5904878/94‑1 (MQ=60)
                                                              AAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAT                                 <  SRR4302532.2044840/94‑1 (MQ=60)
                                                              AAAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAT                                 <  SRR4302532.770625/94‑1 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                <  SRR4302532.127848/94‑1 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                <  SRR4302532.1419707/94‑1 (MQ=60)
                                                               AAACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATG                                >  SRR4302532.5573969/1‑94 (MQ=60)
                                                                AACCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGAGCTAAAACCCCGGTAATGGATGC                               >  SRR4302532.3964193/1‑94 (MQ=60)
                                                                  CCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAC                             >  SRR4302532.5469937/1‑94 (MQ=60)
                                                                  CCTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCAC                             >  SRR4302532.149265/1‑94 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            >  SRR4302532.5118171/1‑94 (MQ=60)
                                                                   CTATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACT                            >  SRR4302532.3989355/1‑94 (MQ=60)
                                                                    TATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTG                           >  SRR4302532.1317949/1‑94 (MQ=60)
                                                                     ATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGT                          >  SRR4302532.2909782/1‑94 (MQ=60)
                                                                     ATGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGT                          >  SRR4302532.6268667/1‑94 (MQ=60)
                                                                      TGGTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTG                         <  SRR4302532.3867727/94‑1 (MQ=60)
                                                                       GGTACTGGTGATTCTGGATTGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGG                        <  SRR4302532.2100302/94‑1 (MQ=60)
                                                                        GTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGG                       <  SRR4302532.3747569/94‑1 (MQ=60)
                                                                        GTACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGG                       >  SRR4302532.557047/1‑94 (MQ=60)
                                                                          ACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCC                     >  SRR4302532.927209/1‑94 (MQ=60)
                                                                          ACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCC                     >  SRR4302532.1279438/1‑94 (MQ=60)
                                                                          ACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCC                     >  SRR4302532.3636853/1‑94 (MQ=60)
                                                                          ACTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCC                     >  SRR4302532.150934/1‑94 (MQ=60)
                                                                           CTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGGCATTTTTAGCGCTACAACCCCGGTAATTGGTGGACTGTGGGGCA                    >  SRR4302532.3132795/1‑94 (MQ=60)
                                                                           CTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGGTGCACTGTGGGCCA                    >  SRR4302532.4283844/1‑94 (MQ=60)
                                                                           CTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCA                    >  SRR4302532.6009683/1‑94 (MQ=60)
                                                                           CTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCA                    >  SRR4302532.3132796/1‑94 (MQ=60)
                                                                           CTGGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCA                    >  SRR4302532.4693214/1‑94 (MQ=60)
                                                                             GGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAT                  <  SRR4302532.3779500/94‑1 (MQ=60)
                                                                             GGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAT                  >  SRR4302532.4804749/1‑94 (MQ=60)
                                                                             GGTGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAAT                  >  SRR4302532.2807576/1‑94 (MQ=60)
                                                                               TGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCG                >  SRR4302532.4104429/1‑94 (MQ=60)
                                                                               TGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCG                >  SRR4302532.4104431/1‑94 (MQ=60)
                                                                               TGATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCG                >  SRR4302532.5750767/1‑94 (MQ=60)
                                                                                GATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGT               <  SRR4302532.1526042/94‑1 (MQ=60)
                                                                                GATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGT               <  SRR4302532.1526043/94‑1 (MQ=60)
                                                                                 ATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTC              >  SRR4302532.2753581/1‑94 (MQ=60)
                                                                                 ATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTC              >  SRR4302532.4091911/1‑94 (MQ=60)
                                                                                 ATTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTC              >  SRR4302532.4681927/1‑94 (MQ=60)
                                                                                  TTCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCAAATCGTCC             >  SRR4302532.5243725/1‑94 (MQ=60)
                                                                                   TCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCG            >  SRR4302532.76607/1‑94 (MQ=60)
                                                                                   TCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCG            <  SRR4302532.2658677/94‑1 (MQ=60)
                                                                                   TCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCG            <  SRR4302532.2370147/94‑1 (MQ=60)
                                                                                   TCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCG            <  SRR4302532.3300359/94‑1 (MQ=60)
                                                                                   TCTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGAAGCACTGTGGGCCAATCGTCCG            <  SRR4302532.6069923/94‑1 (MQ=60)
                                                                                    CTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACGCCTGTAATCGATTCACTGTGGGACAATCGTCCGC           >  SRR4302532.702564/1‑94 (MQ=60)
                                                                                    CTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGC           <  SRR4302532.3011600/94‑1 (MQ=60)
                                                                                    CTGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGC           >  SRR4302532.702563/1‑94 (MQ=60)
                                                                                     TGGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCA          >  SRR4302532.3032746/1‑94 (MQ=60)
                                                                                     TGGATGGCCATGGCTATCGCGAAGAACAGCAGGAAAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCA          >  SRR4302532.5417900/1‑94 (MQ=60)
                                                                                      GGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAT         <  SRR4302532.6095291/94‑1 (MQ=60)
                                                                                      GGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCAT         >  SRR4302532.110245/1‑94 (MQ=60)
                                                                                      GGATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGCCCGCAT         >  SRR4302532.3302540/1‑94 (MQ=60)
                                                                                        ATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAC       <  SRR4302532.4740007/94‑1 (MQ=60)
                                                                                        ATGGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATAC       >  SRR4302532.635207/1‑94 (MQ=60)
                                                                                          GGCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCC     <  SRR4302532.3128561/94‑1 (MQ=60)
                                                                                           GCCATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCT    <  SRR4302532.1876505/94‑1 (MQ=60)
                                                                                             CATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  <  SRR4302532.2548784/94‑1 (MQ=60)
                                                                                             CATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  SRR4302532.1588534/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGTAGTAAAATTACGCAAATTTTGACTCTTGAGTATGAGGTTGTCGCAATGTTGGTTTCTAAAAAACCTATGGTACTGGTGATTCTGGATGGCTATGGCTATCGCGAAGAACAGCAGGATAACGCCATTTTTAGTGCTAAAACCCCGGTAATGGATGCACTGTGGGCCAATCGTCCGCATACCCTAA  >  NC_000913/3782839‑3783025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑