Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,008,525	+G	intergenic (+92/‑141)	b4555 → / → yigM	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,008,524	1	.	G	99.6%	962.4 / ‑3.8	256	intergenic (+91/‑142)	b4555/yigM	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (126/129); total (127/129)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

Only 100 of 273 total aligned reads displayed.

CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611

cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                           <  1:737491/93‑1 (MQ=255)

  ggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                        >  2:172112/1‑92 (MQ=255)

         cgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt                                                                                 <  1:3177658/93‑1 (MQ=255)

         cgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt                                                                                 >  1:5113939/1‑93 (MQ=255)

           acgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTg                                                                               >  2:515563/1‑93 (MQ=255)

                gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACt                                                                          >  2:3294343/1‑93 (MQ=255)

                gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACt                                                                          >  2:608246/1‑93 (MQ=255)

                gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACt                                                                          >  1:1199901/1‑93 (MQ=255)

                   aTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAAt                                                                       <  2:2542082/93‑1 (MQ=255)

                   aTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAAt                                                                       >  2:6487699/1‑93 (MQ=255)

                    tGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg                                                                      >  2:4729384/1‑93 (MQ=255)

                    tGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg                                                                      <  2:5953053/93‑1 (MQ=255)

                      cTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGcc                                                                    >  1:1743840/1‑93 (MQ=255)

                      cTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGcc                                                                    <  1:4629335/93‑1 (MQ=255)

                       tGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg                                                                   >  1:6401371/1‑93 (MQ=255)

                       tGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg                                                                   >  1:3625383/1‑93 (MQ=255)

                       cgTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg                                                                   <  1:506992/92‑1 (MQ=255)

                        gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcga                                                                  <  2:4865780/93‑1 (MQ=255)

                        gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcga                                                                  >  2:1185427/1‑93 (MQ=255)

                         tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgac                                                                 <  2:3782441/93‑1 (MQ=255)

                         tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgac                                                                 <  2:3028737/93‑1 (MQ=255)

                          cGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacg                                                                >  1:523193/1‑93 (MQ=255)

                           gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga                                                               <  2:5635097/93‑1 (MQ=255)

                            aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac                                                              >  1:5701826/1‑93 (MQ=255)

                              ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgc                                                            >  1:4753811/5‑93 (MQ=255)

                               gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg                                                           >  2:452360/4‑93 (MQ=255)

                               gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg                                                           >  1:1820823/4‑93 (MQ=255)

                               gcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg                                                           >  1:558129/4‑93 (MQ=255)

                                cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg                                                          <  1:4029892/91‑1 (MQ=255)

                                cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg                                                          >  1:300252/3‑93 (MQ=255)

                                cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCgg                                                          >  1:2382718/3‑93 (MQ=255)

                                 ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt                                                         >  1:1705896/2‑93 (MQ=255)

                                 ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt                                                         <  1:2670173/92‑1 (MQ=255)

                                 ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt                                                         <  2:3844288/92‑1 (MQ=255)

                                   ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                       >  1:2926756/1‑93 (MQ=255)

                                   ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                       <  2:2055246/93‑1 (MQ=255)

                                   ggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                       <  2:3052035/93‑1 (MQ=255)

                                     aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTGCTAATGCCGACGACGCGGTGCCg                                                     <  1:4697058/89‑1 (MQ=255)

                                      aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                                                    >  2:3675794/4‑93 (MQ=255)

                                       aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                                                   <  2:3364328/91‑1 (MQ=255)

                                       aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                                                   >  2:1312660/3‑93 (MQ=255)

                                           tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               <  2:2126216/93‑1 (MQ=255)

                                           tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               <  1:6465690/93‑1 (MQ=255)

                                            gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              <  1:1378233/93‑1 (MQ=255)

                                            gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              >  2:3730663/1‑93 (MQ=255)

                                             cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  1:6020153/93‑1 (MQ=255)

                                             cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  1:3570102/93‑1 (MQ=255)

                                             cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             >  1:1503404/1‑93 (MQ=255)

                                                  tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        <  2:5587924/93‑1 (MQ=255)

                                                   gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       >  2:1373889/1‑93 (MQ=255)

                                                    ggCAGTGCGCACGAGCGGCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  1:979996/93‑1 (MQ=255)

                                                    ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      >  1:6153694/1‑93 (MQ=255)

                                                     gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc                                     >  2:660382/1‑93 (MQ=255)

                                                      cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct                                    <  1:3048197/93‑1 (MQ=255)

                                                           cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt                               <  2:5340615/91‑1 (MQ=255)

                                                           cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt                               <  2:2328243/91‑1 (MQ=255)

                                                            gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAttt                              <  2:4233000/92‑1 (MQ=255)

                                                             caccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             >  2:5687610/1‑93 (MQ=255)

                                                              accaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                            <  1:100433/93‑1 (MQ=255)

                                                               ccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                           >  1:3834797/1‑93 (MQ=255)

                                                                 aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         >  1:577187/1‑93 (MQ=255)

                                                                 aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         <  1:687844/93‑1 (MQ=255)

                                                                 aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGACGCATTATCTGCGTAAACTCTATTTATCGt                         >  1:2250569/1‑93 (MQ=255)

                                                                  gCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTa                        <  2:1855330/93‑1 (MQ=255)

                                                                   cGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTaa                       <  2:3500554/93‑1 (MQ=255)

                                                                   cGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTaa                       >  1:4310769/1‑93 (MQ=255)

                                                                     tCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                     <  1:4488876/93‑1 (MQ=255)

                                                                      gtGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg                    <  1:1285537/92‑1 (MQ=255)

                                                                      cTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg                    >  2:1951092/1‑93 (MQ=255)

                                                                       tGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   <  1:422988/93‑1 (MQ=255)

                                                                        gAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGtt                  >  1:3517674/1‑93 (MQ=255)

                                                                         aaaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                 >  2:1542161/1‑93 (MQ=255)

                                                                         aaaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                 >  2:584949/1‑93 (MQ=255)

                                                                         aaaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                 >  1:162501/1‑93 (MQ=255)

                                                                          aaaaCCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCt                <  2:5325532/90‑1 (MQ=255)

                                                                          aaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCt                <  1:2129041/93‑1 (MQ=255)

                                                                           aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               <  2:3935201/93‑1 (MQ=255)

                                                                           aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               >  2:1511770/1‑93 (MQ=255)

                                                                            gACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              <  1:629456/93‑1 (MQ=255)

                                                                            gACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  2:2578301/1‑93 (MQ=255)

                                                                             aCCTTTATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             <  1:4923225/93‑1 (MQ=255)

                                                                              ccTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAt            >  1:5424655/1‑93 (MQ=255)

                                                                              ccTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAt            <  2:4409643/93‑1 (MQ=255)

                                                                               cTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           >  1:2760907/1‑93 (MQ=255)

                                                                               cTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAtt           <  2:3238540/93‑1 (MQ=255)

                                                                                ttCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          <  2:3410116/93‑1 (MQ=255)

                                                                                ttCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          <  2:3142386/93‑1 (MQ=255)

                                                                                 tCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         >  1:2860219/1‑93 (MQ=255)

                                                                                 tCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         >  2:2440865/1‑93 (MQ=255)

                                                                                 tCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         <  1:3377039/93‑1 (MQ=255)

                                                                                   aTCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       >  2:1657541/1‑93 (MQ=255)

                                                                                   aTCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       >  2:6184634/1‑93 (MQ=255)

                                                                                    tCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc      <  2:817646/93‑1 (MQ=255)

                                                                                    tCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc      <  2:2171988/93‑1 (MQ=255)

                                                                                    tCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc      >  2:2896225/1‑93 (MQ=255)

                                                                                      ccGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAg    >  2:876994/1‑93 (MQ=255)

                                                                                       cGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGt   <  2:5453072/93‑1 (MQ=255)

                                                                                       cGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGt   >  1:1104611/1‑93 (MQ=255)

                                                                                       cGAGCT‑GTAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGt   >  2:4815951/1‑93 (MQ=255)

                                                                                        gAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  >  2:6440628/1‑93 (MQ=255)

CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618

CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               >  SRR4302547.5830561/1‑94 (MQ=60)

CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               <  SRR4302547.751299/94‑1 (MQ=60)

 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302547.5615165/1‑94 (MQ=60)

 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302547.3976144/1‑94 (MQ=60)

    ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            <  SRR4302547.308281/94‑1 (MQ=60)

     TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                           <  SRR4302547.1089880/94‑1 (MQ=60)

       AACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         >  SRR4302547.1198134/1‑94 (MQ=60)

        tactgcggcataattcattagattggggggaaaggcttaATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         <  SRR4302547.2189780/55‑1 (MQ=34)

        ACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        <  SRR4302547.1788185/94‑1 (MQ=60)

        ACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        >  SRR4302547.5219323/1‑94 (MQ=60)

         CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       <  SRR4302547.3240957/94‑1 (MQ=60)

          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302547.2289714/94‑1 (MQ=60)

          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302547.888404/94‑1 (MQ=60)

            CGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    >  SRR4302547.1230577/1‑94 (MQ=60)

               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302547.1776886/1‑94 (MQ=60)

               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302547.3037007/1‑94 (MQ=60)

               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 <  SRR4302547.469134/94‑1 (MQ=60)

               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302547.1224111/1‑94 (MQ=60)

                GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                >  SRR4302547.5253826/1‑94 (MQ=60)

                 AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302547.544902/1‑94 (MQ=60)

                    TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            <  SRR4302547.6312935/94‑1 (MQ=60)

                     GCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           <  SRR4302547.2982575/94‑1 (MQ=60)

                     GCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           >  SRR4302547.1778203/1‑94 (MQ=60)

                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302547.3606970/94‑1 (MQ=60)

                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302547.4725225/94‑1 (MQ=60)

                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302547.6534897/1‑94 (MQ=60)

                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302547.4185309/1‑94 (MQ=60)

                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302547.3699325/1‑94 (MQ=60)

                       CGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         <  SRR4302547.516194/94‑1 (MQ=60)

                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        <  SRR4302547.5780534/94‑1 (MQ=60)

                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        <  SRR4302547.4348088/94‑1 (MQ=60)

                        GTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        <  SRR4302547.3492111/94‑1 (MQ=60)

                         TCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                                                       >  SRR4302547.532665/1‑94 (MQ=60)

                          CGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                      <  SRR4302547.3621421/94‑1 (MQ=60)

                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302547.6580736/94‑1 (MQ=60)

                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     >  SRR4302547.5819606/1‑94 (MQ=60)

                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     >  SRR4302547.171841/1‑94 (MQ=60)

                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     >  SRR4302547.508670/1‑94 (MQ=60)

                            ATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    >  SRR4302547.5767409/1‑94 (MQ=60)

                             TGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   >  SRR4302547.3747605/1‑94 (MQ=60)

                             TGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   >  SRR4302547.4852321/1‑94 (MQ=60)

                              agtcggggtaaggctgcagtatggtgagtgcggccccagGTCTGGAAGACCCTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   <  SRR4302547.2172320/55‑1 (MQ=46)

                              GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  <  SRR4302547.4499608/94‑1 (MQ=60)

                              GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  >  SRR4302547.568183/1‑94 (MQ=60)

                              GGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  >  SRR4302547.1856817/1‑94 (MQ=60)

                                gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                                                                 >  SRR4302547.305855/11‑94 (MQ=60)

                                gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                                                                 >  SRR4302547.2430988/11‑94 (MQ=60)

                                 cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302547.4111755/85‑1 (MQ=60)

                                 cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                >  SRR4302547.1739451/10‑94 (MQ=60)

                                 GGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                                                              >  SRR4302547.2985126/1‑94 (MQ=60)

                                 GGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                                                              >  SRR4302547.4703879/1‑94 (MQ=60)

                                  ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               <  SRR4302547.2723814/86‑1 (MQ=60)

                                  ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               <  SRR4302547.6443764/86‑1 (MQ=60)

                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          >  SRR4302547.6556496/1‑94 (MQ=60)

                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          >  SRR4302547.2785673/1‑94 (MQ=60)

                                      AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTGCTAATGCCGACGACGCGGTGCCGC                                                           <  SRR4302547.4794351/94‑1 (MQ=60)

                                      AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                                                           >  SRR4302547.5440187/1‑94 (MQ=60)

                                      AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                                                           <  SRR4302547.6712459/94‑1 (MQ=60)

                                        G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        <  SRR4302547.4378874/94‑1 (MQ=60)

                                          GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                       <  SRR4302547.3055973/94‑1 (MQ=60)

                                            TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     <  SRR4302547.6600330/94‑1 (MQ=60)

                                             GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302547.1405862/94‑1 (MQ=60)

                                             GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    >  SRR4302547.1533375/1‑94 (MQ=60)

                                              CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302547.6145100/94‑1 (MQ=60)

                                              CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302547.611167/94‑1 (MQ=60)

                                              CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   >  SRR4302547.999191/1‑94 (MQ=60)

                                              CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302547.3642936/94‑1 (MQ=60)

                                               ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302547.5329233/94‑1 (MQ=60)

                                               ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302547.4473080/94‑1 (MQ=60)

                                                TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302547.1382198/94‑1 (MQ=60)

                                                 TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT                                                >  SRR4302547.614677/1‑94 (MQ=60)

                                                  ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               <  SRR4302547.4875493/94‑1 (MQ=60)

                                                  ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               <  SRR4302547.2308866/94‑1 (MQ=60)

                                                    GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302547.6281375/1‑94 (MQ=60)

                                                    GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302547.1797251/1‑94 (MQ=60)

                                                     GGCAGTGCGCACGAGCGGCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302547.999065/94‑1 (MQ=60)

                                                      GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                           <  SRR4302547.5575843/94‑1 (MQ=60)

                                                       CAGTGCGCACCAGCGTCTGAAAGGCCTTCCTCCCGGGATGGCAGTGTTTGGTTCTCATGCCGACGTCGCGGTGCCGCATTATCTGCGTAAACTC                                          <  SRR4302547.6786390/94‑1 (MQ=23)

                                                       CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC                                          <  SRR4302547.3108841/94‑1 (MQ=60)

                                                        AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         <  SRR4302547.5520832/94‑1 (MQ=60)

                                                        AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         <  SRR4302547.5110872/94‑1 (MQ=60)

                                                        AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         >  SRR4302547.3675527/1‑94 (MQ=60)

                                                         gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302547.4099540/89‑1 (MQ=60)

                                                           gcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      >  SRR4302547.1193913/4‑94 (MQ=60)

                                                             GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302547.1299607/1‑94 (MQ=60)

                                                             GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302547.957844/1‑94 (MQ=60)

                                                              CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   >  SRR4302547.3698978/1‑94 (MQ=60)

                                                               ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302547.1500194/1‑94 (MQ=60)

                                                               ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  <  SRR4302547.102284/94‑1 (MQ=60)

                                                               ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302547.2452061/1‑94 (MQ=60)

                                                               ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302547.3912908/1‑94 (MQ=60)

                                                                 CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302547.587534/1‑94 (MQ=60)

                                                                 CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                <  SRR4302547.2905223/94‑1 (MQ=60)

                                                                 CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302547.2795039/1‑94 (MQ=60)

                                                                 CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGACGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302547.2296003/1‑94 (MQ=60)

                                                                  AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               >  SRR4302547.4954723/1‑94 (MQ=60)

                                                                  AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               <  SRR4302547.700541/94‑1 (MQ=60)

                                                                   GCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAA                              >  SRR4302547.4399447/1‑94 (MQ=60)

                                                                   GCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAA                              <  SRR4302547.2962/94‑1 (MQ=60)

                                                                     GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302547.1600469/1‑94 (MQ=60)

                                                                     GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            <  SRR4302547.5167410/94‑1 (MQ=60)

                                                                     GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302547.5213940/1‑94 (MQ=60)

                                                                      TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302547.4581604/94‑1 (MQ=60)

                                                                       GTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          <  SRR4302547.1311427/94‑1 (MQ=60)

                                                                       CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGTTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          <  SRR4302547.6529243/94‑1 (MQ=60)

                                                                       CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTGATCGT                          >  SRR4302547.1410696/1‑94 (MQ=60)

                                                                       CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          <  SRR4302547.1862551/94‑1 (MQ=60)

                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302547.2255965/94‑1 (MQ=60)

                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302547.430828/94‑1 (MQ=60)

                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302547.3581813/94‑1 (MQ=60)

                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302547.480366/94‑1 (MQ=60)

                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302547.3589478/1‑94 (MQ=60)

                                                                         GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302547.165480/1‑94 (MQ=60)

                                                                           AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302547.2171462/94‑1 (MQ=60)

                                                                           AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302547.726408/94‑1 (MQ=60)

                                                                            AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     >  SRR4302547.2028511/1‑94 (MQ=60)

                                                                             GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302547.641079/94‑1 (MQ=60)

                                                                              ACCTTTATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302547.5025190/94‑1 (MQ=60)

                                                                              ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302547.715732/94‑1 (MQ=60)

                                                                              ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302547.585778/94‑1 (MQ=60)

                                                                              ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   >  SRR4302547.5536840/1‑94 (MQ=60)

                                                                               CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  >  SRR4302547.2816234/1‑94 (MQ=60)

                                                                                CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 <  SRR4302547.3728582/94‑1 (MQ=60)

                                                                                 TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                >  SRR4302547.2917360/1‑94 (MQ=60)

                                                                                 TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                <  SRR4302547.2440660/94‑1 (MQ=60)

                                                                                 TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                <  SRR4302547.3149093/94‑1 (MQ=60)

                                                                                  TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAACCTCTATTTATCGTAATCGTTCTTTATTTGG               <  SRR4302547.3319367/94‑1 (MQ=60)

                                                                                  TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               <  SRR4302547.3924541/94‑1 (MQ=60)

                                                                                  TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               <  SRR4302547.3445653/94‑1 (MQ=60)

                                                                                   CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              <  SRR4302547.6102322/94‑1 (MQ=60)

                                                                                    ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC             <  SRR4302547.3099570/94‑1 (MQ=60)

                                                                                     TCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCA            <  SRR4302547.4799638/94‑1 (MQ=60)

                                                                                     TCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGATGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCA            <  SRR4302547.1711955/94‑1 (MQ=60)

                                                                                      CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302547.1607347/94‑1 (MQ=60)

                                                                                      CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           >  SRR4302547.3320622/1‑94 (MQ=60)

                                                                                      CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302547.6341161/94‑1 (MQ=60)

                                                                                       CCGAGCTGGCAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGT          >  SRR4302547.169154/1‑94 (MQ=60)

                                                                                       CCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGT          >  SRR4302547.1126830/1‑94 (MQ=60)

                                                                                        CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302547.4778131/94‑1 (MQ=60)

                                                                                          AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       <  SRR4302547.568024/94‑1 (MQ=60)

                                                                                            ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302547.4633423/4‑94 (MQ=60)

                                                                                            ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302547.939005/91‑1 (MQ=60)

                                                                                             G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302547.1333410/94‑1 (MQ=60)

                                                                                             G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302547.6148578/94‑1 (MQ=60)

                                                                                             G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   >  SRR4302547.324907/1‑94 (MQ=60)

                                                                                               GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  SRR4302547.1574827/1‑94 (MQ=60)

CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`