Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,156,159 A→G T31A (ACG→GCG)  oxyR → OxyR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,156,1590AG100.0% 948.4 / NA 259T31A (ACG→GCG) oxyROxyR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (133/126);  total (133/126)

Only 100 of 270 total aligned reads displayed.

ATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCG  >  NC_000913/4156069‑4156248
                                                                                          |                                                                                         
aTGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCg                                                                                         >  1:3920625/1‑93 (MQ=255)
  gAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCt                                                                                       >  2:5247173/1‑93 (MQ=255)
   aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCtt                                                                                      <  2:1174114/93‑1 (MQ=255)
    atatTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTa                                                                                     <  2:5012756/93‑1 (MQ=255)
    atatTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTa                                                                                     <  2:5580640/93‑1 (MQ=255)
     tatTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAg                                                                                    >  1:4498432/1‑93 (MQ=255)
     tatTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAg                                                                                    <  1:5673644/93‑1 (MQ=255)
     tatTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAg                                                                                    >  2:3654335/1‑93 (MQ=255)
      atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGc                                                                                   >  2:4412495/1‑93 (MQ=255)
      atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGc                                                                                   >  1:1362491/1‑93 (MQ=255)
         cGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCggg                                                                                <  2:6566136/93‑1 (MQ=255)
          gTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGc                                                                               >  2:1286800/1‑93 (MQ=255)
            gATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCaa                                                                             >  2:1356497/1‑93 (MQ=255)
              tCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAAt                                                                           >  2:5956649/1‑93 (MQ=255)
               cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAAtt                                                                          <  2:5788741/93‑1 (MQ=255)
                ttGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTc                                                                         >  1:3739031/1‑93 (MQ=255)
                 tGAGTCCCTGGTGCCGTTGGCTGAACACCACCGTTTTGGGCGTGCGGCAGATTCCTGCCACGTTAGCTAGCCGGCGCTTAGGGGGCAAATTCg                                                                        <  1:961042/93‑1 (MQ=255)
                  gactACCTGGTGGCATTGGCTGAACACCGCCATTTGCGGCGTGCGGCAGATGCCTGCCACTTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGt                                                                       <  2:5674684/90‑1 (MQ=255)
                  gAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGt                                                                       >  2:6348305/1‑93 (MQ=255)
                  gAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGt                                                                       >  1:4046745/1‑93 (MQ=255)
                  gAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGt                                                                       >  1:1087971/1‑93 (MQ=255)
                   aGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTa                                                                      >  1:407348/1‑93 (MQ=255)
                    gTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTaa                                                                     <  1:2925307/93‑1 (MQ=255)
                      aCCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGTAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGc                                                                   >  2:4681463/1‑93 (MQ=255)
                      aCCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGc                                                                   <  2:2320771/93‑1 (MQ=255)
                       ccTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCt                                                                  <  2:4295230/93‑1 (MQ=255)
                        cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTg                                                                 >  2:6213618/1‑93 (MQ=255)
                         tggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTgg                                                                <  1:2612461/93‑1 (MQ=255)
                           gtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGaa                                                              >  1:1168763/1‑93 (MQ=255)
                             ggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGa                                                            <  1:4582517/93‑1 (MQ=255)
                              gCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAt                                                           <  2:2091986/93‑1 (MQ=255)
                              gCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAt                                                           >  2:3004115/1‑93 (MQ=255)
                              gCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAt                                                           <  1:2344186/93‑1 (MQ=255)
                              gCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGGTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAt                                                           >  1:4772968/1‑93 (MQ=255)
                               cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATg                                                          <  2:2006633/93‑1 (MQ=255)
                               cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATg                                                          <  1:6533450/93‑1 (MQ=255)
                               cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATg                                                          >  1:6284788/1‑93 (MQ=255)
                               cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATg                                                          >  1:1792212/1‑93 (MQ=255)
                                aTTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGa                                                         >  2:5746940/1‑93 (MQ=255)
                                aTTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGa                                                         >  2:5455033/1‑93 (MQ=255)
                                 ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAg                                                        >  2:3691379/1‑93 (MQ=255)
                                  tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                       >  1:3209732/1‑93 (MQ=255)
                                  tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGCAAGCTGGAAGATGAGc                                                       >  2:674558/1‑93 (MQ=255)
                                   ggCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCt                                                      >  1:4399750/1‑93 (MQ=255)
                                    gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTg                                                     >  2:5889408/1‑93 (MQ=255)
                                      tGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTggg                                                   >  1:1471660/1‑93 (MQ=255)
                                       gaccacCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                  <  1:3430966/90‑1 (MQ=255)
                                       gAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGGTGGAAGATGAGCTGGGc                                                  <  1:5272251/93‑1 (MQ=255)
                                       gAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                  >  2:3476088/1‑93 (MQ=255)
                                        aaCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCg                                                 <  1:2803495/93‑1 (MQ=255)
                                          cacCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTg                                               >  2:5639510/1‑93 (MQ=255)
                                           acCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                              >  1:1889605/1‑93 (MQ=255)
                                           acCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                              >  1:6126512/1‑93 (MQ=255)
                                           acCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGACAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                              >  1:1495883/1‑93 (MQ=255)
                                            ccGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAt                                             <  2:903272/93‑1 (MQ=255)
                                            ccGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAt                                             <  1:5229405/93‑1 (MQ=255)
                                               ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGtt                                          >  2:2610489/1‑93 (MQ=255)
                                                 aTTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGc                                        <  1:4655167/93‑1 (MQ=255)
                                                 aTTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGc                                        >  1:5056553/1‑93 (MQ=255)
                                                 aTTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGc                                        <  2:4855253/93‑1 (MQ=255)
                                                  ttttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCt                                       <  1:4364150/93‑1 (MQ=255)
                                                  ttttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCt                                       >  1:5411334/1‑93 (MQ=255)
                                                  ttttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCt                                       <  1:640192/93‑1 (MQ=255)
                                                   tttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTg                                      <  2:6087332/93‑1 (MQ=255)
                                                   tttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTg                                      >  2:809597/1‑93 (MQ=255)
                                                    ttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTgg                                     >  1:3817377/1‑93 (MQ=255)
                                                        gCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCg                                 <  1:4988608/93‑1 (MQ=255)
                                                        gCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCg                                 >  2:3972688/1‑93 (MQ=255)
                                                            gCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAcc                             <  1:742141/93‑1 (MQ=255)
                                                            gCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAcc                             <  1:2738873/93‑1 (MQ=255)
                                                            gCGGCAGATACCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAcc                             <  2:4150669/93‑1 (MQ=255)
                                                             gggCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCa                            <  1:6348829/92‑1 (MQ=255)
                                                             cGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCa                            <  1:4169908/93‑1 (MQ=255)
                                                              ggCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                           >  1:5112406/1‑93 (MQ=255)
                                                               gCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGc                          >  1:2217165/1‑93 (MQ=255)
                                                                 aGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCg                        >  2:5090653/1‑93 (MQ=255)
                                                                   aTTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGGTGAGCTGGGCGTGATGTTGCTGTGGGGGAGCAGCCGTa                      >  1:2431838/1‑93 (MQ=255)
                                                                    ttCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTaa                     >  2:3236399/1‑93 (MQ=255)
                                                                    ttCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTaa                     >  2:5992163/1‑93 (MQ=255)
                                                                      ccTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAg                   >  2:3447779/1‑93 (MQ=255)
                                                                        tGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                 >  1:3560514/1‑93 (MQ=255)
                                                                          ccACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgtt               >  1:3326675/1‑93 (MQ=255)
                                                                          ccACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgtt               >  2:2489819/1‑93 (MQ=255)
                                                                            aCGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttgt             <  2:29169/93‑1 (MQ=255)
                                                                            aCGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttgt             <  1:3662610/93‑1 (MQ=255)
                                                                              gTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTc           <  1:2458902/93‑1 (MQ=255)
                                                                              gTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTc           <  1:5478456/93‑1 (MQ=255)
                                                                              gTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTc           >  2:1663065/1‑93 (MQ=255)
                                                                              gTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTc           >  2:593769/1‑93 (MQ=255)
                                                                               ttAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCa          >  1:6041524/1‑93 (MQ=255)
                                                                                tAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc         >  2:333838/1‑93 (MQ=255)
                                                                                tAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGCAAAGTGTTGTTCAc         >  2:769337/1‑93 (MQ=255)
                                                                                  gccagccGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAccc       <  2:5298918/93‑1 (MQ=255)
                                                                                  gccagccGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAccc       <  2:4076169/93‑1 (MQ=255)
                                                                                   ccagccGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCa      >  2:2699933/1‑93 (MQ=255)
                                                                                     agccGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAgg    >  1:3418824/1‑93 (MQ=255)
                                                                                     agccGGCGCTTAGCGGGCAAATGCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAgg    <  1:2006649/93‑1 (MQ=255)
                                                                                      gccGGCGCTTAGCGGGCAGATTCATAAGCTGGAAGATGAGCTGGGGGTAATGTTGCTGGAGCGAACCAGCCGTAAAGTGTTGTTCACCCAGGc   <  1:3525113/93‑1 (MQ=255)
                                                                                      gccGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGGGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGc   >  2:4508971/1‑93 (MQ=255)
                                                                                      gccGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGc   <  2:1824534/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCG  >  NC_000913/4156069‑4156248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGG  >  NC_000913/4156066‑4156250
                                                                                             |                                                                                           
ATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGG                                                                                             >  SRR4302547.1229754/1‑94 (MQ=60)
 TAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGC                                                                                            <  SRR4302547.2660331/94‑1 (MQ=60)
 TAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGC                                                                                            >  SRR4302547.5289232/1‑94 (MQ=60)
  AATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGGTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCG                                                                                           <  SRR4302547.220007/94‑1 (MQ=60)
  AATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCG                                                                                           >  SRR4302547.4000346/1‑94 (MQ=60)
     GAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTT                                                                                        >  SRR4302547.3943975/1‑94 (MQ=60)
     GAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTT                                                                                        >  SRR4302547.4700646/1‑94 (MQ=60)
       ATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAG                                                                                      >  SRR4302547.4591350/1‑94 (MQ=60)
        TATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGC                                                                                     >  SRR4302547.1389830/1‑94 (MQ=60)
        TATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGC                                                                                     <  SRR4302547.5790794/94‑1 (MQ=60)
         ATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCG                                                                                    >  SRR4302547.1585500/1‑94 (MQ=60)
         ATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCG                                                                                    <  SRR4302547.5623396/94‑1 (MQ=60)
          TTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGG                                                                                   <  SRR4302547.635523/94‑1 (MQ=60)
           TCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGG                                                                                  <  SRR4302547.3360053/94‑1 (MQ=60)
            CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGC                                                                                 >  SRR4302547.6715265/1‑94 (MQ=60)
             GTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCA                                                                                >  SRR4302547.5321781/1‑94 (MQ=60)
             GTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCA                                                                                >  SRR4302547.5817245/1‑94 (MQ=60)
             GTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCA                                                                                >  SRR4302547.6526787/1‑94 (MQ=60)
               GATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAA                                                                              >  SRR4302547.2070322/1‑94 (MQ=60)
                ATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAAT                                                                             >  SRR4302547.4136128/1‑94 (MQ=60)
                  CTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTG                                                                           >  SRR4302547.2765376/1‑94 (MQ=60)
                  CTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTC                                                                           <  SRR4302547.4248184/94‑1 (MQ=60)
                  CTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTC                                                                           >  SRR4302547.3815369/1‑94 (MQ=60)
                   TTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCG                                                                          <  SRR4302547.3587414/94‑1 (MQ=60)
                    TGAGTCCCTGGTGCCGTTGGCTGAACACCACCGTTTTGGGCGTGCGGCAGATTCCTGCCACGTTAGCTAGCCGGCGCTTAGGGGGCAAATTCGT                                                                         <  SRR4302547.979706/94‑1 (MQ=53)
                    TGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGT                                                                         >  SRR4302547.1109737/1‑94 (MQ=60)
                    TGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGT                                                                         >  SRR4302547.4128920/1‑94 (MQ=60)
                    TGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGGTAGCCAGCCGGCGCTTAGCTGGCAAATTCGT                                                                         >  SRR4302547.3000613/1‑94 (MQ=60)
                     GAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTA                                                                        >  SRR4302547.414904/1‑94 (MQ=60)
                     GAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTA                                                                        <  SRR4302547.2675246/94‑1 (MQ=60)
                      AGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAA                                                                       <  SRR4302547.467374/94‑1 (MQ=60)
                      AGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAA                                                                       <  SRR4302547.51157/94‑1 (MQ=60)
                      AGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAA                                                                       >  SRR4302547.4903136/1‑94 (MQ=60)
                       GTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAG                                                                      <  SRR4302547.6272220/94‑1 (MQ=60)
                       GTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAG                                                                      <  SRR4302547.2983660/94‑1 (MQ=60)
                        TACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGGAGATTCCTGCCACGTTAATCATTCGTCGCTTAGAGGGCAAATTCGTAAGC                                                                     >  SRR4302547.4614427/1‑94 (MQ=60)
                        TACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGC                                                                     <  SRR4302547.5533170/94‑1 (MQ=60)
                         TCCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCT                                                                    <  SRR4302547.2376305/94‑1 (MQ=60)
                          CCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTG                                                                   >  SRR4302547.616807/1‑94 (MQ=60)
                           CTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGG                                                                  >  SRR4302547.3595045/1‑94 (MQ=60)
                           CTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGG                                                                  >  SRR4302547.1457055/1‑94 (MQ=60)
                            TGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGA                                                                 <  SRR4302547.2665102/94‑1 (MQ=60)
                            TGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGA                                                                 <  SRR4302547.5220462/94‑1 (MQ=60)
                             GGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAA                                                                >  SRR4302547.5105531/1‑94 (MQ=60)
                             GGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAA                                                                >  SRR4302547.1192397/1‑94 (MQ=60)
                             GGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAA                                                                <  SRR4302547.3713928/94‑1 (MQ=60)
                              GTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAG                                                               <  SRR4302547.3112028/94‑1 (MQ=60)
                                GGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAT                                                             <  SRR4302547.4677354/94‑1 (MQ=60)
                                GGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAT                                                             <  SRR4302547.6462160/94‑1 (MQ=60)
                                GGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAT                                                             <  SRR4302547.3799916/94‑1 (MQ=60)
                                GGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGGTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGAT                                                             >  SRR4302547.4871856/1‑94 (MQ=60)
                                 GCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATG                                                            >  SRR4302547.6415366/1‑94 (MQ=60)
                                 GCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATG                                                            >  SRR4302547.1827695/1‑94 (MQ=60)
                                 GCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATG                                                            <  SRR4302547.2391691/94‑1 (MQ=60)
                                  CATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGA                                                           <  SRR4302547.6669429/94‑1 (MQ=60)
                                  CATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGA                                                           <  SRR4302547.1470151/94‑1 (MQ=60)
                                   ATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAG                                                          <  SRR4302547.3488756/94‑1 (MQ=60)
                                   ATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAG                                                          <  SRR4302547.2465521/94‑1 (MQ=60)
                                   ATGGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAG                                                          <  SRR4302547.1750087/94‑1 (MQ=60)
                                    TTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGC                                                         <  SRR4302547.2457606/94‑1 (MQ=60)
                                    TTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGC                                                         >  SRR4302547.2959026/1‑94 (MQ=60)
                                    TTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGC                                                         >  SRR4302547.3273774/1‑94 (MQ=60)
                                     TGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCT                                                        >  SRR4302547.3500384/1‑94 (MQ=60)
                                     TGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCT                                                        >  SRR4302547.4490349/1‑94 (MQ=60)
                                     TGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCT                                                        >  SRR4302547.1918132/1‑94 (MQ=60)
                                        CTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAACTTCGTAAGCTGGAAGATGAGCTGGG                                                     >  SRR4302547.5346319/1‑94 (MQ=60)
                                        CTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGG                                                     >  SRR4302547.1501055/1‑94 (MQ=60)
                                          GACCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCG                                                   <  SRR4302547.3500877/94‑1 (MQ=60)
                                          GAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGGTGGAAGATGAGCTGGGCG                                                   <  SRR4302547.5380894/94‑1 (MQ=60)
                                          GAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCG                                                   >  SRR4302547.2795333/1‑94 (MQ=60)
                                           AACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGGAGATGAGCTGGGCGT                                                  >  SRR4302547.2483888/1‑94 (MQ=60)
                                           AACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGT                                                  <  SRR4302547.2859618/94‑1 (MQ=60)
                                            ACCCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTG                                                 <  SRR4302547.3728882/94‑1 (MQ=60)
                                             CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTTGTAAGCTGGAAGATGAGCTGGGCGTGA                                                >  SRR4302547.2609386/1‑94 (MQ=60)
                                             CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGA                                                >  SRR4302547.1926948/1‑94 (MQ=60)
                                             CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGA                                                >  SRR4302547.6253600/1‑94 (MQ=60)
                                             CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGACAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGA                                                >  SRR4302547.1525723/1‑94 (MQ=60)
                                               CCGCTATTTGCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATG                                              <  SRR4302547.401465/94‑1 (MQ=60)
                                               CCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATG                                              <  SRR4302547.5337050/94‑1 (MQ=60)
                                                CGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGT                                             >  SRR4302547.12782/1‑94 (MQ=60)
                                                   CATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGC                                          >  SRR4302547.5160916/1‑94 (MQ=60)
                                                    ATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCT                                         >  SRR4302547.5523252/1‑94 (MQ=60)
                                                    ATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCT                                         <  SRR4302547.4751581/94‑1 (MQ=60)
                                                     TTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTG                                        <  SRR4302547.652003/94‑1 (MQ=60)
                                                     TTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTG                                        <  SRR4302547.4453769/94‑1 (MQ=60)
                                                      TTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGG                                       >  SRR4302547.3895162/1‑94 (MQ=60)
                                                      TTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGG                                       <  SRR4302547.6660678/94‑1 (MQ=60)
                                                       ttcgacgtgctgtgGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAGTTCGTAAGCTGGAAGATGAGCTGGGCGTTATGTTGCTGGA                                      <  SRR4302547.465826/80‑1 (MQ=60)
                                                       TTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGA                                      <  SRR4302547.1362930/94‑1 (MQ=60)
                                                       TTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGA                                      <  SRR4302547.6453827/94‑1 (MQ=60)
                                                           GCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGG                                  <  SRR4302547.5091736/94‑1 (MQ=60)
                                                           GCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGG                                  <  SRR4302547.34656/94‑1 (MQ=60)
                                                            CGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGA                                 >  SRR4302547.3784167/1‑94 (MQ=60)
                                                             GTGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAC                                >  SRR4302547.1916043/1‑94 (MQ=60)
                                                              TGCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACC                               <  SRR4302547.805621/94‑1 (MQ=60)
                                                               GCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA                              <  SRR4302547.756087/94‑1 (MQ=60)
                                                               GCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA                              >  SRR4302547.4460107/1‑94 (MQ=60)
                                                               GCGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA                              <  SRR4302547.2793825/94‑1 (MQ=60)
                                                                GGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAG                             <  SRR4302547.6481108/94‑1 (MQ=60)
                                                                CGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAG                             <  SRR4302547.1505549/94‑1 (MQ=60)
                                                                CGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAG                             >  SRR4302547.5217760/1‑94 (MQ=60)
                                                                CGGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAG                             <  SRR4302547.4254839/94‑1 (MQ=60)
                                                                 GGCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGC                            >  SRR4302547.2261823/1‑94 (MQ=60)
                                                                  GCATATTCCTG‑CACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCG                          <  SRR4302547.5367145/94‑1 (MQ=60)
                                                                  GCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCG                           >  SRR4302547.3459060/1‑94 (MQ=60)
                                                                  GCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCC                           <  SRR4302547.4933573/94‑1 (MQ=60)
                                                                  GCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCC                           <  SRR4302547.1191919/94‑1 (MQ=60)
                                                                  GCAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCC                           <  SRR4302547.4205323/94‑1 (MQ=60)
                                                                   CAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTGAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCG                          >  SRR4302547.4486479/1‑94 (MQ=60)
                                                                   CAGATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCG                          <  SRR4302547.2020064/94‑1 (MQ=60)
                                                                     GATTCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGGTGAGCTGGGCGTGATGTTGCTGTGGGGGAGCAGCCGTA                        >  SRR4302547.2481036/1‑94 (MQ=60)
                                                                        tcctgcgagtTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAG                     <  SRR4302547.4335683/84‑1 (MQ=60)
                                                                        TCCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAG                     <  SRR4302547.917003/94‑1 (MQ=60)
                                                                         CCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGT                    >  SRR4302547.4045470/1‑94 (MQ=60)
                                                                         CCTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGG                    >  SRR4302547.3994437/1‑94 (MQ=60)
                                                                          CTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTG                   >  SRR4302547.3633166/1‑94 (MQ=60)
                                                                          CTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTG                   >  SRR4302547.5439272/1‑94 (MQ=60)
                                                                          CTGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTG                   >  SRR4302547.5736104/1‑94 (MQ=60)
                                                                           TGCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGT                  >  SRR4302547.223680/1‑94 (MQ=60)
                                                                            GCCACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTT                 >  SRR4302547.3393902/1‑94 (MQ=60)
                                                                             CCACGTTAGCCAGCCGGCGCTCAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTG                >  SRR4302547.3850261/1‑94 (MQ=60)
                                                                               ACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTT              <  SRR4302547.3737331/94‑1 (MQ=60)
                                                                               ACGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGAACCAGCCGTAAAGTGTTGTT              <  SRR4302547.6628320/94‑1 (MQ=60)
                                                                                CGTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTC             <  SRR4302547.6710068/94‑1 (MQ=60)
                                                                                 GTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCA            >  SRR4302547.6166886/1‑94 (MQ=60)
                                                                                 GTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCA            <  SRR4302547.2527479/94‑1 (MQ=60)
                                                                                 GTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCA            <  SRR4302547.2949311/94‑1 (MQ=60)
                                                                                 GTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCA            <  SRR4302547.2508589/94‑1 (MQ=60)
                                                                                 GTTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCA            <  SRR4302547.5591591/94‑1 (MQ=60)
                                                                                 GTTAGCCAGCCGGCGCTTAGCGGGCAAATTAGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCA            >  SRR4302547.3625524/1‑94 (MQ=60)
                                                                                  TTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAGCAGCCGTAAAGTGTTGTTCAC           >  SRR4302547.2343827/1‑94 (MQ=60)
                                                                                  TTAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAC           >  SRR4302547.4814874/1‑94 (MQ=60)
                                                                                   TAGCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACC          >  SRR4302547.457716/1‑94 (MQ=60)
                                                                                     GCCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCA        <  SRR4302547.5919803/94‑1 (MQ=60)
                                                                                      CCAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAG       >  SRR4302547.5190785/1‑94 (MQ=60)
                                                                                       CAGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGG      >  SRR4302547.3488432/1‑94 (MQ=60)
                                                                                        AGCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGC     >  SRR4302547.5430363/1‑94 (MQ=60)
                                                                                        AGCCGGCGCTTAGCGGGCAAATGCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGC     <  SRR4302547.2046579/94‑1 (MQ=60)
                                                                                        AGACGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGC     >  SRR4302547.4271836/1‑94 (MQ=60)
                                                                                         GCCGGCGCTTAGCGGGCAGATTCATAAGCTGGAAGATGAGCTGGGGGTAATGTTGCTGGAGCGAACCAGCCGTAAAGTGTTGTTCACCCAGGCG    <  SRR4302547.3597075/94‑1 (MQ=60)
                                                                                         GCCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCG    <  SRR4302547.4302718/94‑1 (MQ=60)
                                                                                          CCGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGG   >  SRR4302547.6446571/1‑94 (MQ=60)
                                                                                          CCGGCGCTTAGCGGGCAAATTCGAAAGCTGGAAGATGAGCTGGGGGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGG   <  SRR4302547.3422852/94‑1 (MQ=60)
                                                                                           CGGCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGG  >  SRR4302547.6536385/1‑94 (MQ=60)
                                                                                           CGGCGCTGAGCGGACAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTCTTCACCCAGGCGGG  <  SRR4302547.2610731/94‑1 (MQ=60)
                                                                                             |                                                                                           
ATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGG  >  NC_000913/4156066‑4156250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑