Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,388,615	A→G	V158A (GTG→GCG)	b4161 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,388,615	0	A	G	100.0%	1013.8 / NA	272	V158A (GTG→GCG)	b4161	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (142/130); total (142/130)

Only 100 of 276 total aligned reads displayed.

AATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA  >  NC_000913/4388525‑4388704

  tACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaat                                                                                       <  2:6343085/93‑1 (MQ=255)

  tACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaat                                                                                       >  1:2715587/1‑93 (MQ=255)

     gcgATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaataat                                                                                    <  2:4616656/93‑1 (MQ=255)

     gcgATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCaataat                                                                                    >  1:205773/1‑93 (MQ=255)

       gATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCg                                                                                  <  1:5673054/93‑1 (MQ=255)

       gATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCg                                                                                  >  1:654018/1‑93 (MQ=255)

         tAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGc                                                                                >  2:5976519/1‑93 (MQ=255)

           aCCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTc                                                                              >  1:4776617/1‑93 (MQ=255)

            ccGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCa                                                                             >  2:240326/1‑93 (MQ=255)

            ccGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGGCTATCTTGTTGAGTACAATAATCGACTCa                                                                             >  2:6258064/1‑93 (MQ=255)

             cGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCaa                                                                            <  1:1079135/93‑1 (MQ=255)

             cGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCaa                                                                            <  2:1209480/93‑1 (MQ=255)

              gATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAAt                                                                           >  2:5259228/1‑93 (MQ=255)

              gATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAAt                                                                           >  2:4916520/1‑93 (MQ=255)

               atatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATc                                                                          >  2:2766115/1‑93 (MQ=255)

               atatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATc                                                                          <  2:312150/93‑1 (MQ=255)

                tatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCt                                                                         <  2:5830978/93‑1 (MQ=255)

                tatTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCt                                                                         <  2:4165726/93‑1 (MQ=255)

                  ttGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGc                                                                       >  2:3423239/1‑93 (MQ=255)

                  ttGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGc                                                                       <  1:5100544/93‑1 (MQ=255)

                    gCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCaa                                                                     <  2:3061886/93‑1 (MQ=255)

                    gCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCaa                                                                     <  2:1346573/93‑1 (MQ=255)

                    gCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCaa                                                                     <  1:2754854/93‑1 (MQ=255)

                     cGGTAGATATCCATCTCCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAg                                                                    <  1:41529/93‑1 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  1:5727381/1‑93 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  2:171241/1‑93 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  2:1951765/1‑93 (MQ=255)

                      ggTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAgg                                                                   >  1:2942800/1‑93 (MQ=255)

                       gTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGt                                                                  <  1:1671236/93‑1 (MQ=255)

                        tAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGtt                                                                 <  1:3299183/93‑1 (MQ=255)

                         aGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGgtt                                                                >  1:3789172/1‑93 (MQ=255)

                          gATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTc                                                               >  2:4641212/1‑93 (MQ=255)

                          gATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTc                                                               <  2:4494429/93‑1 (MQ=255)

                           atatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCg                                                              <  1:3694918/93‑1 (MQ=255)

                           atatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCg                                                              >  2:5484683/1‑93 (MQ=255)

                            tatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGc                                                             >  1:1774443/1‑93 (MQ=255)

                             atCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCa                                                            <  1:6278271/93‑1 (MQ=255)

                              tCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAg                                                           >  1:6045635/1‑93 (MQ=255)

                              tCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAg                                                           <  2:2599727/93‑1 (MQ=255)

                               ccATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAgg                                                          <  2:654421/93‑1 (MQ=255)

                               ccATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGGCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAgg                                                          >  1:2025737/1‑93 (MQ=255)

                                cATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGc                                                         <  2:5310058/93‑1 (MQ=255)

                                  tCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGcca                                                       >  2:1994027/1‑93 (MQ=255)

                                    tGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGccacc                                                     >  1:5417746/1‑93 (MQ=255)

                                    tGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGccacc                                                     >  1:978159/1‑93 (MQ=255)

                                     gCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGccacca                                                    >  2:5597920/1‑93 (MQ=255)

                                      cTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAg                                                   >  2:3666436/1‑93 (MQ=255)

                                      cTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAg                                                   <  1:2680618/93‑1 (MQ=255)

                                       tCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAgg                                                  <  2:2974747/93‑1 (MQ=255)

                                         gTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTa                                                >  2:1280590/1‑93 (MQ=255)

                                         gTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTa                                                >  1:3477470/1‑93 (MQ=255)

                                          ttGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTaa                                               <  2:453057/93‑1 (MQ=255)

                                           tGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAAc                                              <  2:5695228/93‑1 (MQ=255)

                                           tGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAAc                                              <  2:1412093/93‑1 (MQ=255)

                                             aCGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACgg                                            >  1:277591/1‑93 (MQ=255)

                                                aaCGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCg                                         <  1:302540/93‑1 (MQ=255)

                                                  cGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGat                                       <  1:1461834/93‑1 (MQ=255)

                                                    ccATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGataa                                     >  2:1248220/1‑93 (MQ=255)

                                                     cATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGataat                                    >  1:5175074/1‑93 (MQ=255)

                                                      aTGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGataata                                   >  2:4359553/1‑93 (MQ=255)

                                                        gCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATAtt                                 <  2:6589551/93‑1 (MQ=255)

                                                        gCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATAtt                                 >  1:1558561/1‑93 (MQ=255)

                                                         ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTg                                <  1:4049433/93‑1 (MQ=255)

                                                         ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTg                                <  1:83126/93‑1 (MQ=255)

                                                         ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTg                                >  2:377393/1‑93 (MQ=255)

                                                          cTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGa                               <  1:2160777/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              <  1:5034221/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              <  1:3745126/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              <  2:5157980/93‑1 (MQ=255)

                                                           ttCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAg                              >  2:1540228/1‑93 (MQ=255)

                                                              gtcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGa                           >  2:2299697/1‑93 (MQ=255)

                                                              gtcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGa                           <  2:5448501/93‑1 (MQ=255)

                                                              gtcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGa                           >  2:6085272/1‑93 (MQ=255)

                                                               tcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAc                          <  1:5982691/93‑1 (MQ=255)

                                                               tcgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAc                          >  1:2541401/1‑93 (MQ=255)

                                                                cgtcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACa                         <  1:5372380/93‑1 (MQ=255)

                                                                  tcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                       <  2:1655062/93‑1 (MQ=255)

                                                                  tcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                       >  1:1859799/1‑93 (MQ=255)

                                                                  tcCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                       >  2:2903174/1‑93 (MQ=255)

                                                                   ccAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCt                      >  1:4494987/1‑93 (MQ=255)

                                                                     agcagGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTcc                    <  2:6512647/93‑1 (MQ=255)

                                                                      gcagGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCg                   <  2:1769664/93‑1 (MQ=255)

                                                                      gcagGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCg                   <  2:6438037/93‑1 (MQ=255)

                                                                           tCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaa              >  2:3216130/1‑93 (MQ=255)

                                                                            cTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa             <  1:5299564/93‑1 (MQ=255)

                                                                            cTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa             >  2:3865933/1‑93 (MQ=255)

                                                                            cTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa             >  1:1836216/1‑93 (MQ=255)

                                                                             tATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAt            >  1:1249796/1‑93 (MQ=255)

                                                                             tATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAt            >  1:2412346/1‑93 (MQ=255)

                                                                             tATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAt            >  1:5642279/1‑93 (MQ=255)

                                                                               tCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATgg          >  2:2046740/1‑93 (MQ=255)

                                                                                cTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGc         >  1:1003024/1‑93 (MQ=255)

                                                                                cTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGc         <  1:6613745/93‑1 (MQ=255)

                                                                                 ttgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCg        >  2:1066572/1‑93 (MQ=255)

                                                                                 ttgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCg        >  2:4042242/1‑93 (MQ=255)

                                                                                  tgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCgg       >  1:2145041/1‑93 (MQ=255)

                                                                                  tgttgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCgg       >  1:157830/1‑93 (MQ=255)

                                                                                     tgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGgaga    <  2:501439/93‑1 (MQ=255)

                                                                                     tgAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGgaga    <  1:4401722/93‑1 (MQ=255)

                                                                                      gAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAgac   >  2:3316895/1‑93 (MQ=255)

AATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA  >  NC_000913/4388525‑4388704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGA  >  NC_000913/4388522‑4388708

ATCAATACGCGATAACCGATATTGCGGTAAATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCG                                                                                               <  SRR4302547.1702534/94‑1 (MQ=60)

 TCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGC                                                                                              >  SRR4302547.6348795/1‑94 (MQ=60)

 TCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGC                                                                                              >  SRR4302547.3574332/1‑94 (MQ=60)

  CAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCA                                                                                             <  SRR4302547.2520137/94‑1 (MQ=60)

  CAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCA                                                                                             >  SRR4302547.6058944/1‑94 (MQ=60)

  CAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCA                                                                                             <  SRR4302547.1469352/94‑1 (MQ=60)

  CAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCA                                                                                             <  SRR4302547.4343805/94‑1 (MQ=60)

    ATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAAT                                                                                           >  SRR4302547.2770100/1‑94 (MQ=60)

      ACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAA                                                                                         <  SRR4302547.1598800/94‑1 (MQ=60)

       CGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAAT                                                                                        >  SRR4302547.209635/1‑94 (MQ=60)

       CGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAAT                                                                                        <  SRR4302547.1814126/94‑1 (MQ=60)

        GCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATC                                                                                       >  SRR4302547.248191/1‑94 (MQ=60)

         CGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCG                                                                                      >  SRR4302547.4919275/1‑94 (MQ=60)

         CGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCG                                                                                      <  SRR4302547.609452/94‑1 (MQ=60)

         CGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCG                                                                                      >  SRR4302547.666065/1‑94 (MQ=60)

          GATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGG                                                                                     <  SRR4302547.3713940/94‑1 (MQ=60)

          GATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGG                                                                                     <  SRR4302547.5790191/94‑1 (MQ=60)

           ATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGC                                                                                    <  SRR4302547.3851097/94‑1 (MQ=60)

             AACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTC                                                                                  >  SRR4302547.4875574/1‑94 (MQ=60)

               CCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAA                                                                                >  SRR4302547.1312527/1‑94 (MQ=60)

                CGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAAT                                                                               <  SRR4302547.1100666/94‑1 (MQ=60)

                  ATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCT                                                                             <  SRR4302547.2748665/94‑1 (MQ=60)

                  ATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTATATCTTGTTGAGCGCAATAATCGGCTCAATCT                                                                             >  SRR4302547.580546/1‑94 (MQ=60)

                   TATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTG                                                                            >  SRR4302547.2821446/1‑94 (MQ=60)

                    ATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGC                                                                           >  SRR4302547.5558419/1‑94 (MQ=60)

                    ATTGCGGAAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCGGGTCTATCTTGTTGATCGCAATAATCGGCTCAATCTGC                                                                           >  SRR4302547.1028039/1‑94 (MQ=60)

                     TTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCA                                                                          <  SRR4302547.5205702/94‑1 (MQ=60)

                       GCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAG                                                                        <  SRR4302547.3050526/94‑1 (MQ=60)

                       GCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAG                                                                        <  SRR4302547.88799/94‑1 (MQ=60)

                       GCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAG                                                                        <  SRR4302547.2810077/94‑1 (MQ=60)

                       GCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAG                                                                        <  SRR4302547.5389604/94‑1 (MQ=60)

                        CGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGG                                                                       >  SRR4302547.3001426/1‑94 (MQ=60)

                        CGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGG                                                                       >  SRR4302547.2189593/1‑94 (MQ=60)

                        CGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGG                                                                       >  SRR4302547.5845696/1‑94 (MQ=60)

                        CGGTAGATATCCATCTCCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGG                                                                       <  SRR4302547.42293/94‑1 (MQ=60)

                         GGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGT                                                                      >  SRR4302547.2125973/1‑94 (MQ=60)

                          GTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTT                                                                     <  SRR4302547.1704159/94‑1 (MQ=60)

                          GTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTT                                                                     >  SRR4302547.3786410/1‑94 (MQ=60)

                          GTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTT                                                                     <  SRR4302547.4630597/94‑1 (MQ=60)

                           TAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTT                                                                    <  SRR4302547.3365578/94‑1 (MQ=60)

                           TAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGGTT                                                                    >  SRR4302547.3866427/1‑94 (MQ=60)

                            AGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTC                                                                   <  SRR4302547.1794085/94‑1 (MQ=60)

                             GATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCG                                                                  <  SRR4302547.1889838/94‑1 (MQ=60)

                              ATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGC                                                                 <  SRR4302547.3770343/94‑1 (MQ=60)

                              ATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGC                                                                 <  SRR4302547.5480909/94‑1 (MQ=60)

                              ATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGC                                                                 >  SRR4302547.1809637/1‑94 (MQ=60)

                                ATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAG                                                               <  SRR4302547.5169406/94‑1 (MQ=60)

                                ATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAG                                                               >  SRR4302547.3910923/1‑94 (MQ=60)

                                ATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAG                                                               >  SRR4302547.6171072/1‑94 (MQ=60)

                                ATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAG                                                               <  SRR4302547.6408706/94‑1 (MQ=60)

                                ATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTATTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAG                                                               <  SRR4302547.736078/94‑1 (MQ=60)

                                 TCCATCTGTTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGG                                                              >  SRR4302547.5484484/1‑94 (MQ=60)

                                 TCCATCTGCTCGTTGACGATTGCCATGCCTTCGTCGTCCAGCAGGTCTGTCTTTTTGAGCGCAATAATaggttcacactgcaagtgttcgcaga                                                              >  SRR4302547.3257460/1‑68 (MQ=54)

                                 TCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGGCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGG                                                              >  SRR4302547.2066081/1‑94 (MQ=60)

                                   CATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCC                                                            <  SRR4302547.6199423/94‑1 (MQ=60)

                                    ATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCA                                                           >  SRR4302547.6583028/1‑94 (MQ=60)

                                    ATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCA                                                           >  SRR4302547.5779867/1‑94 (MQ=60)

                                      CTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACC                                                         >  SRR4302547.5529818/1‑94 (MQ=60)

                                      CTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACC                                                         >  SRR4302547.997179/1‑94 (MQ=60)

                                       TGCTCGTTGCCGAACGCCATGTCTTCGTCGTCCACCAGGTCTATCTTGTTGAGCGCAATAATCGGACCAATCTGCTAGGTGTCGCAGGCCACCA                                                        >  SRR4302547.4722878/1‑94 (MQ=60)

                                       TGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCA                                                        <  SRR4302547.6728338/94‑1 (MQ=60)

                                         CTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGG                                                      <  SRR4302547.2734445/94‑1 (MQ=60)

                                         CTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGG                                                      <  SRR4302547.6305741/94‑1 (MQ=60)

                                           CGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTA                                                    >  SRR4302547.3548401/1‑94 (MQ=60)

                                              TGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACG                                                 >  SRR4302547.2215202/1‑94 (MQ=60)

                                               GACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGG                                                <  SRR4302547.1525144/94‑1 (MQ=60)

                                               GACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGG                                                <  SRR4302547.5840159/94‑1 (MQ=60)

                                               GACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGG                                                <  SRR4302547.25364/94‑1 (MQ=60)

                                               GACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGG                                                >  SRR4302547.282773/1‑94 (MQ=60)

                                               GACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGG                                                <  SRR4302547.1670834/94‑1 (MQ=60)

                                                   AACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGA                                            <  SRR4302547.2075955/94‑1 (MQ=60)

                                                   AACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGA                                            <  SRR4302547.308181/94‑1 (MQ=60)

                                                   AACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGA                                            >  SRR4302547.66849/1‑94 (MQ=60)

                                                    ACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGAT                                           >  SRR4302547.2143254/1‑94 (MQ=60)

                                                     CGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATA                                          <  SRR4302547.1664427/94‑1 (MQ=60)

                                                     CGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATA                                          <  SRR4302547.1491048/94‑1 (MQ=60)

                                                      GCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAA                                         <  SRR4302547.4791053/94‑1 (MQ=60)

                                                      GCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAA                                         >  SRR4302547.2241271/1‑94 (MQ=60)

                                                       CCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAAT                                        >  SRR4302547.5281605/1‑94 (MQ=60)

                                                        CATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATA                                       >  SRR4302547.1573973/1‑94 (MQ=60)

                                                          TGCCTTCGTCGTCCATCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATT                                     <  SRR4302547.1849219/94‑1 (MQ=60)

                                                          TGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATT                                     >  SRR4302547.1589605/1‑94 (MQ=60)

                                                          TGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATT                                     >  SRR4302547.4015867/1‑94 (MQ=60)

                                                           GCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTG                                    >  SRR4302547.1265725/1‑94 (MQ=60)

                                                            GCTTCGGCGTCTAGCAGGTCTATCTTGTTGAGGTGAATAGTTGGCTCAATCTGCAAGGTTTCGGAGGCCGTCACGTAACGGTCGATAAGATTGG                                   <  SRR4302547.1312408/94‑1 (MQ=19)

                                                            CCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGA                                   <  SRR4302547.5432182/94‑1 (MQ=60)

                                                            CCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGA                                   <  SRR4302547.84665/94‑1 (MQ=60)

                                                            CCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGA                                   <  SRR4302547.4131674/94‑1 (MQ=60)

                                                             CTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAG                                  <  SRR4302547.2203963/94‑1 (MQ=60)

                                                             CTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAG                                  <  SRR4302547.2906419/94‑1 (MQ=60)

                                                              TTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGC                                 <  SRR4302547.5138219/94‑1 (MQ=60)

                                                              TTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGC                                 <  SRR4302547.3821575/94‑1 (MQ=60)

                                                              TTCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGC                                 <  SRR4302547.270210/94‑1 (MQ=60)

                                                               TCGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCG                                <  SRR4302547.3180886/94‑1 (MQ=60)

                                                                CGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGA                               <  SRR4302547.4332016/94‑1 (MQ=60)

                                                                CGTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGA                               <  SRR4302547.345249/94‑1 (MQ=60)

                                                                 GTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAC                              <  SRR4302547.3695582/94‑1 (MQ=60)

                                                                 GTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAC                              <  SRR4302547.2821539/94‑1 (MQ=60)

                                                                 GTCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGAC                              >  SRR4302547.2592739/1‑94 (MQ=60)

                                                                  TCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACA                             >  SRR4302547.5504006/1‑94 (MQ=60)

                                                                  TCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACA                             <  SRR4302547.850573/94‑1 (MQ=60)

                                                                  TCGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACA                             <  SRR4302547.6106798/94‑1 (MQ=60)

                                                                   CGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAG                            <  SRR4302547.4175203/94‑1 (MQ=60)

                                                                   CGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAG                            <  SRR4302547.5483252/94‑1 (MQ=60)

                                                                   CGTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAG                            <  SRR4302547.2025243/94‑1 (MQ=60)

                                                                    GTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGC                           >  SRR4302547.2008760/1‑94 (MQ=60)

                                                                    GTCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGC                           >  SRR4302547.1896522/1‑94 (MQ=60)

                                                                     TCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCT                          >  SRR4302547.4587826/1‑94 (MQ=60)

                                                                     TCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCT                          <  SRR4302547.3289933/94‑1 (MQ=60)

                                                                     TCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCT                          >  SRR4302547.14829/1‑94 (MQ=60)

                                                                     TCCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCT                          <  SRR4302547.416630/94‑1 (MQ=60)

                                                                      CCAGCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTC                         <  SRR4302547.2785257/94‑1 (MQ=60)

                                                                         GCAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGG                      >  SRR4302547.6025453/1‑94 (MQ=60)

                                                                          CAGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGC                     >  SRR4302547.3782579/1‑94 (MQ=60)

                                                                           AGGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCA                    >  SRR4302547.4272850/1‑94 (MQ=60)

                                                                            GGTCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAA                   >  SRR4302547.1753256/1‑94 (MQ=60)

                                                                             GTCTATCTTGTTGAGCGCAATAATCGGCTCAATTTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAA                  >  SRR4302547.5163490/1‑94 (MQ=60)

                                                                              TCTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAA                 >  SRR4302547.1872516/1‑94 (MQ=60)

                                                                               CTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAT                <  SRR4302547.5408911/94‑1 (MQ=60)

                                                                               CTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAT                >  SRR4302547.5758751/1‑94 (MQ=60)

                                                                               CTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAT                >  SRR4302547.1275015/1‑94 (MQ=60)

                                                                               CTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAT                <  SRR4302547.1232234/94‑1 (MQ=60)

                                                                               CTATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAAT                >  SRR4302547.2461185/1‑94 (MQ=60)

                                                                                 ATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGG              >  SRR4302547.2907550/1‑94 (MQ=60)

                                                                                 ATCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGG              >  SRR4302547.3514490/1‑94 (MQ=60)

                                                                                  TCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGC             >  SRR4302547.1022592/1‑94 (MQ=60)

                                                                                  TCTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGC             <  SRR4302547.5315952/94‑1 (MQ=60)

                                                                                   CTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCG            <  SRR4302547.5787527/94‑1 (MQ=60)

                                                                                   CTTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCG            <  SRR4302547.6751406/94‑1 (MQ=60)

                                                                                    TTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGG           >  SRR4302547.2187828/1‑94 (MQ=60)

                                                                                    TTGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGG           >  SRR4302547.160721/1‑94 (MQ=60)

                                                                                     TGTTGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGA          >  SRR4302547.4574508/1‑94 (MQ=60)

                                                                                       TTGAGCGCAATAATCGGCTCAATCTGCAAGGTTGCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGA        <  SRR4302547.6592340/94‑1 (MQ=60)

                                                                                        TGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGAC       <  SRR4302547.5261879/94‑1 (MQ=60)

                                                                                        TGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGAC       <  SRR4302547.4492364/94‑1 (MQ=60)

                                                                                        TGAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGAC       <  SRR4302547.2089526/94‑1 (MQ=60)

                                                                                         GAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA      <  SRR4302547.5034497/94‑1 (MQ=60)

                                                                                         GAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA      >  SRR4302547.2686221/1‑94 (MQ=60)

                                                                                         GAGCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACA      <  SRR4302547.2774553/94‑1 (MQ=60)

                                                                                           GCGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATAGAGCGACAGCTCCGGCAAAATGGCGGAGACAAT    >  SRR4302547.3644395/1‑94 (MQ=60)

                                                                                            CGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATG   <  SRR4302547.1162450/94‑1 (MQ=60)

                                                                                            CGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATG   <  SRR4302547.3226883/94‑1 (MQ=60)

                                                                                            CGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATG   >  SRR4302547.2570708/1‑94 (MQ=60)

                                                                                            CGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATG   >  SRR4302547.2143648/1‑94 (MQ=60)

                                                                                            CGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATG   <  SRR4302547.5466698/94‑1 (MQ=60)

                                                                                            CGCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATA   >  SRR4302547.4996168/1‑94 (MQ=60)

                                                                                             GCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGA  <  SRR4302547.4734127/94‑1 (MQ=60)

                                                                                             GCAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGA  <  SRR4302547.296114/94‑1 (MQ=60)

                                                                                             GCAATAATCGGCTCAATCTGCAAGGGTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGA  >  SRR4302547.5750051/1‑94 (MQ=60)

ATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGA  >  NC_000913/4388522‑4388708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`