Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I122 R1
|
193 |
20.9 |
1228102 |
94.7% |
1163012 |
84.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
4,263,211 |
T→C |
K197E (AAA→GAA) |
uvrA ← |
excinuclease ABC subunit UvrA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 4,263,211 | 0 | T | C | 100.0%
| 137.8
/ NA
| 37 | K197E (AAA→GAA) | uvrA | excinuclease ABC subunit UvrA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/15); total (22/15) |
GCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTTCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT > NZ_CP009273/4263129‑4263297
|
gcgGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGc < 1:29137/90‑1 (MQ=255)
gcgGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGc < 1:217804/90‑1 (MQ=255)
ggTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAg > 2:183445/1‑90 (MQ=255)
ttCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTc > 2:471369/1‑90 (MQ=255)
cGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCg < 2:415863/90‑1 (MQ=255)
gTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGaaa < 2:97781/90‑1 (MQ=255)
tCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACt < 1:482209/90‑1 (MQ=255)
gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc > 1:540995/1‑90 (MQ=255)
gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc > 2:599353/1‑90 (MQ=255)
gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc > 1:136992/1‑90 (MQ=255)
gTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTc > 1:210170/1‑90 (MQ=255)
tCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCg < 1:291596/90‑1 (MQ=255)
cACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGc < 2:210499/90‑1 (MQ=255)
gCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCAt < 1:177577/90‑1 (MQ=255)
ccTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCaa > 1:21226/1‑90 (MQ=255)
ttGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATa > 2:488220/1‑90 (MQ=255)
cGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCa > 1:419880/1‑90 (MQ=255)
gATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc < 2:141391/90‑1 (MQ=255)
gATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc < 1:471369/90‑1 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc > 2:201679/1‑89 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCAc < 1:201679/89‑1 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg > 1:128722/1‑90 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg > 1:608488/1‑90 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg < 2:157443/90‑1 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg > 2:168501/1‑90 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg > 1:389298/1‑90 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg < 1:488220/90‑1 (MQ=255)
aTCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACg > 2:227088/1‑90 (MQ=255)
ccaccaCTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGt < 1:64697/90‑1 (MQ=255)
accacTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAg > 2:573494/1‑90 (MQ=255)
acTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGccc > 1:427170/1‑90 (MQ=255)
cTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCt > 2:77967/1‑90 (MQ=255)
tATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCa < 2:338848/90‑1 (MQ=255)
aTGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAg > 1:183180/1‑90 (MQ=255)
aTGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAg > 1:428816/1‑90 (MQ=255)
aTGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAg > 1:21436/1‑90 (MQ=255)
gTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGt > 2:266/1‑90 (MQ=255)
|
GCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTTCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT > NZ_CP009273/4263129‑4263297
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCGGAAAGCTCCAGCGCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTTCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT > NZ_CP009273/4263113‑4263297
|
ACCGGAAAGCTCCAGCGCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCC < SRR3721989.538225/100‑1 (MQ=60)
GCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTT < SRR3721989.221749/100‑1 (MQ=60)
GCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTT < SRR3721989.29646/100‑1 (MQ=60)
GTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGctgtctcttat > SRR3721989.129332/1‑89 (MQ=60)
GGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTC > SRR3721989.139478/1‑100 (MQ=60)
GGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTC > SRR3721989.552652/1‑100 (MQ=60)
GGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTC > SRR3721989.213976/1‑100 (MQ=60)
TCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAA > SRR3721989.21594/1‑100 (MQ=60)
TCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAA < SRR3721989.492369/100‑1 (MQ=60)
TCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATAC < SRR3721989.296986/100‑1 (MQ=60)
GCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGC < SRR3721989.180825/100‑1 (MQ=60)
CACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCA > SRR3721989.428524/1‑100 (MQ=60)
CCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACG > SRR3721989.131083/1‑100 (MQ=60)
CCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACG > SRR3721989.621688/1‑100 (MQ=60)
CCTTGAAGCGATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACG > SRR3721989.397152/1‑100 (MQ=60)
GATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCC < SRR3721989.481282/100‑1 (MQ=60)
GATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCC < SRR3721989.205350/100‑1 (MQ=60)
ATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCC > SRR3721989.436015/1‑100 (MQ=60)
ATCAACCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCC < SRR3721989.498534/100‑1 (MQ=60)
CCACCACTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCT < SRR3721989.65841/100‑1 (MQ=60)
CTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAG > SRR3721989.437707/1‑100 (MQ=60)
CTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAG > SRR3721989.21806/1‑100 (MQ=60)
CTTCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAG > SRR3721989.186541/1‑100 (MQ=60)
TCAATGGTATGTTCCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT > SRR3721989.234845/1‑100 (MQ=60)
|
ACCGGAAAGCTCCAGCGCGGTTTCAAATGACTCGGCAAGACGTTGGGTAAGATCGTCACGCACCTTGAAGCGATCAACCACCACTTCAATGGTATGTTTCTTTTGCAGTTCCAGTTTTGGCGGATCGGAAAGATCGCAGACTTCGCCATCAATACGAGCACGGATGTAGCCCTGGCTTGCCAGGT > NZ_CP009273/4263113‑4263297
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
