Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I75 R1
|
71 |
32.8 |
2150506 |
93.3% |
2006422 |
83.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
3,845,141 |
A→G |
I335T (ATC→ACC) |
ilvB ← |
acetolactate synthase large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 3,845,141 | 0 | A | G | 100.0%
| 124.8
/ NA
| 35 | I335T (ATC→ACC) | ilvB | acetolactate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/21); total (14/21) |
GCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATC > NZ_CP009273/3845054‑3845226
|
gCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTc > 1:1001489/1‑90 (MQ=255)
cTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCa < 1:432507/90‑1 (MQ=255)
ttCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAAc < 1:656611/90‑1 (MQ=255)
gACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCa < 1:499780/90‑1 (MQ=255)
gACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGAGCCAGCa < 1:3798/90‑1 (MQ=255)
acacGGAAACTCCCGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCAc < 2:86640/90‑1 (MQ=255)
acacGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCAc < 2:140231/90‑1 (MQ=255)
accTCACGCTGCAAATCCGCTACCAACTGGTGCCCCTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTTCGCCAGCACGtcatca < 2:1060254/88‑1 (MQ=255)
aaCTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGGCAACTGCGCCAGCACGtcatca > 2:316594/1‑90 (MQ=255)
tGCAAACCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCAACAGCGGGGTCAACTGCGCCAGCACGTCCTCAACGTCCGcc < 2:250197/90‑1 (MQ=255)
aaaTCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGa > 2:9838/1‑90 (MQ=255)
gCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCTcc > 1:313996/1‑90 (MQ=255)
aaCTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGc > 1:201480/1‑90 (MQ=255)
cTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCgg < 1:422127/90‑1 (MQ=255)
ggTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgct > 1:975681/1‑90 (MQ=255)
ggTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgct > 2:625883/1‑90 (MQ=255)
tGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgctgc < 2:201480/90‑1 (MQ=255)
ccACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt < 2:1034257/90‑1 (MQ=255)
cACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTg > 1:632722/1‑90 (MQ=255)
aCTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGa > 1:691225/1‑90 (MQ=255)
tctGCACGCGGTTGCGCTTCCACCATCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt > 2:437801/1‑86 (MQ=255)
tctGCACGCGGTTGCGCTTCCACCATCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt < 1:437801/86‑1 (MQ=255)
gCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTa > 1:947228/1‑90 (MQ=255)
gCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTa > 1:43466/1‑90 (MQ=255)
cgcgGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGATGCTTGATTTTAccc < 1:463735/90‑1 (MQ=255)
cgGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt > 1:650013/1‑78 (MQ=255)
cgGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt < 2:650013/78‑1 (MQ=255)
ggTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGc < 2:1001489/90‑1 (MQ=255)
cTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCAc < 2:405383/90‑1 (MQ=255)
tCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACgg > 2:501980/1‑90 (MQ=255)
caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc < 2:281090/90‑1 (MQ=255)
caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc < 1:9838/90‑1 (MQ=255)
caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc < 2:883101/90‑1 (MQ=255)
caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc < 1:971290/90‑1 (MQ=255)
cGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATc < 2:152332/90‑1 (MQ=255)
|
GCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATC > NZ_CP009273/3845054‑3845226
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
