Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F2 I74 R1	158	13.7	815430	95.7%	780366	86.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,662,649	T→G	H58P (CAC→CCC)	mlc ←	DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,662,649	0	T	G	100.0%	25.9 / NA	9	H58P (CAC→CCC)	mlc	DNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (3/6); total (3/6)

GTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACT  >  NZ_CP009273/1662564‑1662732

gTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGc                                                                                 >  1:342866/1‑90 (MQ=255)

       gCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGc                                                                          <  1:164097/90‑1 (MQ=255)

               ttCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACg                                                                  >  1:53402/1‑90 (MQ=255)

                                     ggACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCa                                            <  2:176865/90‑1 (MQ=255)

                                                          tcttTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACgg                       >  1:11997/1‑90 (MQ=255)

                                                                aTTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGa                 <  1:283903/90‑1 (MQ=255)

                                                                         tctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAAATCGATAcgc        <  2:53402/90‑1 (MQ=255)

                                                                          ctTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATAcgcg       <  2:2054/90‑1 (MQ=255)

                                                                               aCCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACt  <  2:257767/90‑1 (MQ=255)

GTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACT  >  NZ_CP009273/1662564‑1662732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCAGAGAAAGATAGTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGA  >  NZ_CP009273/1662551‑1662735

GCAGAGAAAGATAGTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGG                                                                                       <  SRR3722212.47871/100‑1 (MQ=60)

   GAGAAAGATAGTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGC                                                                                    >  SRR3722212.347643/1‑100 (MQ=60)

                  AGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACG                                                                     >  SRR3722212.54039/1‑100 (MQ=60)

                    GCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGA                                                                   <  SRR3722212.165937/100‑1 (MQ=60)

                                                             GTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGG                          >  SRR3722212.12145/1‑100 (MQ=60)

                                                                             ATTTCCAGCTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCG          <  SRR3722212.287682/100‑1 (MQ=60)

                                                                                     CTCTTGCACCAGGGGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGA  >  SRR3722212.189592/1‑100 (MQ=60)

GCAGAGAAAGATAGTGCCAGGCTTCAGTTTCAACCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGA  >  NZ_CP009273/1662551‑1662735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`