Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I205 R1
|
220 |
17.5 |
961422 |
97.1% |
933540 |
86.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,174,381 |
T→G |
C270G (TGC→GGC) |
yegU → |
ADP‑ribosylglycohydrolase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,174,381 | 0 | T | G | 100.0%
| 64.6
/ NA
| 20 | C270G (TGC→GGC) | yegU | ADP‑ribosylglycohydrolase family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (9/11); total (9/11) |
GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGC > NZ_CP009273/2174296‑2174454
|
ggTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGgcgc < 2:121590/90‑1 (MQ=255)
ggTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGgcgc < 1:98127/90‑1 (MQ=255)
gTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGcc > 2:50314/1‑90 (MQ=255)
cAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCt > 1:269697/1‑90 (MQ=255)
cTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTggcg > 1:43635/1‑90 (MQ=255)
gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGacac > 2:442757/1‑90 (MQ=255)
gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGacac < 1:468467/90‑1 (MQ=255)
gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGACCTGTGCGCTAACCTTGGCGGCGacac > 2:468467/1‑90 (MQ=255)
gttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGac < 1:193661/90‑1 (MQ=255)
ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca < 2:405505/90‑1 (MQ=255)
ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca > 1:405505/1‑90 (MQ=255)
ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca > 2:5598/1‑90 (MQ=255)
tccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGacac < 2:94814/90‑1 (MQ=255)
gtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCAt > 1:356156/1‑90 (MQ=255)
tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc < 1:442757/90‑1 (MQ=255)
tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc < 2:43635/90‑1 (MQ=255)
ggTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca < 2:155903/71‑1 (MQ=255)
ggTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca > 1:155903/1‑71 (MQ=255)
ttGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACgg < 2:269697/90‑1 (MQ=255)
aaCCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGgcgc < 1:448603/90‑1 (MQ=255)
|
GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGC > NZ_CP009273/2174296‑2174454
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGC > NZ_CP009273/2174296‑2174464
|
GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGC < SRR3722087.99069/100‑1 (MQ=60)
GCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCT > SRR3722087.272351/1‑100 (MQ=60)
GGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCG > SRR3722087.43985/1‑100 (MQ=60)
ATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACA > SRR3722087.410215/1‑100 (MQ=60)
AGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCAT > SRR3722087.360163/1‑100 (MQ=60)
GTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATC < SRR3722087.473919/100‑1 (MQ=60)
GTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTG < SRR3722087.195363/100‑1 (MQ=60)
TGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGG < SRR3722087.447933/100‑1 (MQ=60)
CCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCctgtctctt > SRR3722087.157336/1‑91 (MQ=60)
AACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGC < SRR3722087.453838/100‑1 (MQ=60)
|
GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGC > NZ_CP009273/2174296‑2174464
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
