Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I211 R1
|
223 |
18.0 |
1007644 |
96.3% |
970361 |
85.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,375,251 |
A→G |
G55G (GGA→GGG) |
rbn → |
ribonuclease BN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,375,251 | 0 | A | G | 100.0%
| 62.0
/ NA
| 20 | G55G (GGA→GGG) | rbn | ribonuclease BN |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/12); total (8/12) |
CAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCA > NZ_CP009273/2375165‑2375335
|
cAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGgaaa > 1:443057/1‑90 (MQ=255)
cAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGgaaa > 1:55814/1‑90 (MQ=255)
aaCATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAAc < 2:335764/90‑1 (MQ=255)
ccGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGAc < 1:491995/90‑1 (MQ=255)
gACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACaa < 1:502726/90‑1 (MQ=255)
gagCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGAtttt > 2:414725/1‑90 (MQ=255)
gACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCa > 1:194109/1‑90 (MQ=255)
tttGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTc < 1:87016/90‑1 (MQ=255)
tttGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTc < 2:47382/90‑1 (MQ=255)
tttGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTc < 2:469517/90‑1 (MQ=255)
tgttTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCCGTCACCTTc > 1:347208/1‑90 (MQ=255)
gttTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCa > 1:3119/1‑90 (MQ=255)
gCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGatc > 1:6632/1‑90 (MQ=255)
cccAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGtt > 1:298589/1‑90 (MQ=255)
gCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTAcc < 2:134903/90‑1 (MQ=255)
aGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCt < 2:298589/90‑1 (MQ=255)
aGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCt < 2:444316/90‑1 (MQ=255)
aGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCt < 2:54063/90‑1 (MQ=255)
aTACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGAGCATCTTTTTGGTTTACCCGGCTTGCTGTGCa > 1:351649/1‑90 (MQ=255)
aCCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGt < 2:188747/90‑1 (MQ=255)
tttAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCt > 1:174048/1‑90 (MQ=255)
ccTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCa < 2:471288/90‑1 (MQ=255)
|
CAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCA > NZ_CP009273/2375165‑2375335
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCA > NZ_CP009273/2375155‑2375346
|
GCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAA > SRR3722094.450836/1‑100 (MQ=60)
GCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAA > SRR3722094.56587/1‑100 (MQ=60)
CTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAAC < SRR3722094.146042/100‑1 (MQ=60)
CCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTA < SRR3722094.500667/100‑1 (MQ=60)
GACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATC < SRR3722094.511585/100‑1 (MQ=60)
ACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCA > SRR3722094.196758/1‑100 (MQ=60)
GGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCCGTCACCTTC > SRR3722094.352971/1‑100 (MQ=60)
GACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCA > SRR3722094.3166/1‑100 (MQ=60)
TTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGG < SRR3722094.88269/100‑1 (MQ=60)
TTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATC > SRR3722094.6735/1‑100 (MQ=60)
GGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTT > SRR3722094.303154/1‑100 (MQ=60)
CAGCTACTGCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGAGCATCTTTTTGGTTTACCCGGCTTGCTGTGCA > SRR3722094.357520/1‑100 (MQ=60)
GCATACCGCCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCT > SRR3722094.176462/1‑100 (MQ=60)
CCTTTAACCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGG > SRR3722094.424317/1‑100 (MQ=60)
CCCTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATC > SRR3722094.474128/1‑100 (MQ=60)
CTGGGAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCA > SRR3722094.122755/1‑100 (MQ=60)
|
GCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAGATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCA > NZ_CP009273/2375155‑2375346
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
