Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I211 R1
|
223 |
18.0 |
1007644 |
96.3% |
970361 |
85.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,410,868 |
A→T |
G103G (GGA→GGT) |
yfcC → |
putative basic amino acid antiporter YfcC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,410,868 | 0 | A | T | 100.0%
| 50.0
/ NA
| 15 | G103G (GGA→GGT) | yfcC | putative basic amino acid antiporter YfcC |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (6/9); total (6/9) |
CTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA > NZ_CP009273/2410783‑2410953
|
cTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGa < 2:105649/90‑1 (MQ=255)
tATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATAc < 1:401226/90‑1 (MQ=255)
aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGa > 2:277785/1‑90 (MQ=255)
aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGa > 2:464846/1‑90 (MQ=255)
ccGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGAc < 2:102469/90‑1 (MQ=255)
gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg > 2:345693/1‑90 (MQ=255)
ggCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGttt > 2:35482/1‑90 (MQ=255)
gggCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTgg > 1:262527/1‑90 (MQ=255)
aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGca < 1:342148/54‑1 (MQ=255)
aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGca > 2:342148/1‑54 (MQ=255)
cccGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAt < 1:444054/90‑1 (MQ=255)
tttGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg < 2:80171/90‑1 (MQ=255)
ggATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGt < 1:440237/90‑1 (MQ=255)
cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCa < 1:223414/90‑1 (MQ=255)
cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCa < 2:409074/90‑1 (MQ=255)
|
CTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA > NZ_CP009273/2410783‑2410953
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGG > NZ_CP009273/2410788‑2410963
|
TATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCG < SRR3722094.408151/100‑1 (MQ=60)
cagcgtcagatgtgtataagagacaGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTT < SRR3722094.347794/75‑1 (MQ=60)
ATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGG > SRR3722094.266422/1‑100 (MQ=60)
CCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGT < SRR3722094.451857/100‑1 (MQ=60)
GGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA < SRR3722094.447953/100‑1 (MQ=60)
CAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGG < SRR3722094.226412/100‑1 (MQ=60)
|
TATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGG > NZ_CP009273/2410788‑2410963
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
