Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I225 R1
|
227 |
21.4 |
1179702 |
97.1% |
1145490 |
86.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,410,868 |
A→T |
G103G (GGA→GGT) |
yfcC → |
putative basic amino acid antiporter YfcC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,410,868 | 0 | A | T | 100.0%
| 99.9
/ NA
| 29 | G103G (GGA→GGT) | yfcC | putative basic amino acid antiporter YfcC |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (16/13); total (16/13) |
ACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA > NZ_CP009273/2410781‑2410953
|
aCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTc > 2:184454/1‑90 (MQ=255)
gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg > 2:393822/1‑90 (MQ=255)
gcgTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAg > 2:226311/1‑90 (MQ=255)
gTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGcc > 1:94888/1‑90 (MQ=255)
cAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGtt > 2:463814/1‑90 (MQ=255)
tGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGca < 2:73092/90‑1 (MQ=255)
gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat > 1:289193/1‑90 (MQ=255)
gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat > 2:237028/1‑90 (MQ=255)
gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGcat > 1:50018/1‑90 (MQ=255)
acgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATg > 1:21390/1‑90 (MQ=255)
acgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATg > 2:272031/1‑90 (MQ=255)
gggCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGtt < 1:581744/90‑1 (MQ=255)
ggCGATGAACGCCCGGGCCGGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGtgt > 1:69133/1‑88 (MQ=255)
gCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa > 1:311081/1‑90 (MQ=255)
ccTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg < 1:237028/90‑1 (MQ=255)
ccTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTggcgg < 1:393822/90‑1 (MQ=255)
aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt < 1:536353/89‑1 (MQ=255)
aCTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt > 2:536353/1‑89 (MQ=255)
cccGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAt < 1:463814/90‑1 (MQ=255)
cGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATG‑TTATGCTGGTGATTGGCGGCGCGTTTGGCATtgt > 2:279002/1‑90 (MQ=255)
tttGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg < 2:80871/90‑1 (MQ=255)
gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg < 1:272031/90‑1 (MQ=255)
gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg < 1:24762/90‑1 (MQ=255)
gAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg < 1:205400/90‑1 (MQ=255)
ggATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATG‑TTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTa < 1:279002/90‑1 (MQ=255)
aaCCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAgg > 1:393236/1‑90 (MQ=255)
cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg > 2:495976/1‑60 (MQ=255)
cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGgcggcg < 1:495976/60‑1 (MQ=255)
cAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCa < 2:180461/90‑1 (MQ=255)
|
ACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCA > NZ_CP009273/2410781‑2410953
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAT > NZ_CP009273/2410787‑2410954
|
GTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCC > SRR3722109.96066/1‑100 (MQ=60)
GCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCAT > SRR3722109.50605/1‑100 (MQ=60)
GCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCAT > SRR3722109.292888/1‑100 (MQ=60)
GCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATG > SRR3722109.21669/1‑100 (MQ=60)
GTTCACGACGGGCGATGAACGCCCGGGCCGGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTGT > SRR3722109.69963/1‑100 (MQ=60)
GGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTG < SRR3722109.590552/100‑1 (MQ=60)
GGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGA > SRR3722109.315159/1‑100 (MQ=60)
CCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGC < SRR3722109.399471/100‑1 (MQ=60)
CCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGC < SRR3722109.239826/100‑1 (MQ=60)
cagatgtgtataagagacagGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAT < SRR3722109.503465/80‑1 (MQ=60)
AACTTCCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGA < SRR3722109.544514/100‑1 (MQ=60)
CCCGTTTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGT < SRR3722109.470778/100‑1 (MQ=60)
TTGAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGG > SRR3722109.398875/1‑100 (MQ=60)
GAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAA < SRR3722109.25077/100‑1 (MQ=60)
GAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAA < SRR3722109.275422/100‑1 (MQ=60)
GAAGGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAA < SRR3722109.207890/100‑1 (MQ=60)
GGATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATG‑TTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAT < SRR3722109.282518/100‑1 (MQ=60)
GATTAACCTCAGGTTCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAT > SRR3722109.24322/1‑100 (MQ=60)
|
GTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAT > NZ_CP009273/2410787‑2410954
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
