Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I194 R1
|
197 |
32.2 |
1845014 |
95.2% |
1756453 |
85.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,868,213 |
T→C |
F128S (TTC→TCC) |
yeaK → |
mischarged aminoacyl‑tRNA deacylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,868,213 | 0 | T | C | 100.0%
| 76.0
/ NA
| 22 | F128S (TTC→TCC) | yeaK | mischarged aminoacyl‑tRNA deacylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/9); total (13/9) |
TCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGC > NZ_CP009273/1868135‑1868299
|
tCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTtg > 1:708605/1‑90 (MQ=255)
gtgtCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCg > 2:692045/1‑90 (MQ=255)
ttCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTc < 1:356459/90‑1 (MQ=255)
tCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCa > 1:111612/1‑90 (MQ=255)
cgcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGc > 1:250195/1‑90 (MQ=255)
cgcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGc > 2:762753/1‑90 (MQ=255)
gcgATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCa > 2:374576/1‑90 (MQ=255)
ccccTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGc < 2:708605/90‑1 (MQ=255)
aaaCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAAcc > 2:750690/1‑90 (MQ=255)
aaCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcg > 1:170177/1‑90 (MQ=255)
aCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcgc < 2:777103/90‑1 (MQ=255)
aCTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACcgc < 2:358676/90‑1 (MQ=255)
ggTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGa > 2:69404/1‑90 (MQ=255)
gTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat > 2:383883/1‑90 (MQ=255)
gTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGat > 2:446800/1‑90 (MQ=255)
ccTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgc > 1:549443/1‑90 (MQ=255)
ctctACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgca < 1:314349/90‑1 (MQ=255)
tctACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcat < 1:374576/90‑1 (MQ=255)
ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg > 1:139825/1‑90 (MQ=255)
ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg < 2:49043/90‑1 (MQ=255)
ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg < 1:446800/90‑1 (MQ=255)
ctctCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcg < 1:416780/90‑1 (MQ=255)
tctcCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATttgcgcattgcgc < 1:82081/90‑1 (MQ=255)
|
TCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGC > NZ_CP009273/1868135‑1868299
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTCGATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGG > NZ_CP009273/1868125‑1868311
|
GTCGATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTG > SRR3722072.721244/1‑100 (MQ=60)
GGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGctgtctcttatacacatctc > SRR3722072.306719/1‑80 (MQ=60)
GGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCA > SRR3722072.113676/1‑100 (MQ=60)
GTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGC > SRR3722072.254426/1‑100 (MQ=60)
TTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCA < SRR3722072.362766/100‑1 (MQ=60)
CCAAAACTCAAACTGGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCG > SRR3722072.173124/1‑100 (MQ=60)
GGTTGCCGACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGC > SRR3722072.559327/1‑100 (MQ=60)
gacCCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCG > SRR3722072.142333/4‑100 (MQ=60)
ACCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCA > SRR3722072.53818/1‑100 (MQ=60)
CCCTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAA > SRR3722072.928727/1‑100 (MQ=60)
CTCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACC < SRR3722072.319838/100‑1 (MQ=60)
TCTACTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCA < SRR3722072.381249/100‑1 (MQ=60)
CTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAAC < SRR3722072.424331/100‑1 (MQ=60)
CTCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAAC < SRR3722072.454985/100‑1 (MQ=60)
TCTCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACT < SRR3722072.83622/100‑1 (MQ=60)
TCCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGG < SRR3722072.349199/100‑1 (MQ=60)
|
GTCGATGAACTCACAGGCTGTGTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGG > NZ_CP009273/1868125‑1868311
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
