Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I97 R1
|
275 |
44.0 |
1733530 |
96.5% |
1672856 |
122.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
592,602 |
C→T |
Q6* (CAA→TAA) |
cusF → |
Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 592,602 | 0 | C | T | 100.0%
| 123.0
/ NA
| 32 | Q6* (CAA→TAA) | cusF | Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16) |
TCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTA > NZ_CP009273/592470‑592718
|
tctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCg > 1:267811/1‑139 (MQ=255)
gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAg < 2:665597/133‑1 (MQ=255)
gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAg > 1:665597/1‑133 (MQ=255)
cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGc < 2:247297/136‑1 (MQ=255)
cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGc < 1:715186/136‑1 (MQ=255)
cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAAt > 2:97433/1‑139 (MQ=255)
cgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAAt < 1:97674/139‑1 (MQ=255)
aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGt < 2:310004/137‑1 (MQ=255)
aGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTa < 2:631996/139‑1 (MQ=255)
gTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTAc < 2:660423/139‑1 (MQ=255)
acttacttATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCg < 2:26646/138‑1 (MQ=255)
tAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCttt > 2:49159/1‑138 (MQ=255)
gCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATg > 1:842387/1‑139 (MQ=255)
gATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTa < 2:129571/139‑1 (MQ=255)
aCTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGaa > 2:407391/1‑139 (MQ=255)
gCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcat < 1:276608/138‑1 (MQ=255)
cGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcatcatcat > 2:822368/1‑138 (MQ=255)
gCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATg < 1:863753/138‑1 (MQ=255)
aaTTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGca > 2:758315/1‑139 (MQ=255)
cTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAg < 1:717874/139‑1 (MQ=255)
ttAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAgcgc > 2:169418/1‑139 (MQ=255)
ttAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAgcgc > 1:217746/1‑139 (MQ=255)
aGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcat < 1:730516/87‑1 (MQ=255)
aGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcat > 2:730516/1‑87 (MQ=255)
aGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATg < 2:626290/94‑1 (MQ=255)
aGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATg > 1:626290/1‑94 (MQ=255)
aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAg > 2:632008/1‑37 (MQ=255)
aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAg < 1:632008/37‑1 (MQ=255)
aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAgcgc > 2:344929/1‑124 (MQ=255)
aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAgcgc < 1:344929/124‑1 (MQ=255)
aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTa > 2:707469/1‑137 (MQ=255)
aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTa > 1:817466/1‑137 (MQ=255)
|
TCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTA > NZ_CP009273/592470‑592718
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAAAGGGTATCGATCTGGAAAGCAAAAAA > NZ_CP009273/592459‑592745
|
CATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGC > SRR3722120.271652/1‑151 (MQ=60)
GTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAAT > SRR3722120.674727/1‑151 (MQ=60)
GCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATG < SRR3722120.405699/150‑1 (MQ=60)
GCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGT < SRR3722120.98973/151‑1 (MQ=60)
GCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTC < SRR3722120.724935/148‑1 (MQ=60)
gttttgttatttcttttttttttttcaagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagCTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCG < SRR3722120.640747/59‑1 (MQ=60)
TACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGC > SRR3722120.853718/1‑151 (MQ=60)
cgccttagtctcgtgggctcggagatgtgtataagagacagCTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAAC < SRR3722120.740413/109‑1 (MQ=60)
CGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAAC < SRR3722120.280592/150‑1 (MQ=60)
CGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGC < SRR3722120.875289/150‑1 (MQ=60)
acgagattcgccttagtctcgtgggctcggagatgtgtataagagacagAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAG < SRR3722120.450140/102‑1 (MQ=60)
GAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCC > SRR3722120.220799/1‑151 (MQ=60)
ACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCG < SRR3722120.727647/151‑1 (MQ=60)
CTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCctgtctcttatacacatctccgagcccacgagact > SRR3722120.634976/1‑116 (MQ=60)
gacagCTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGG < SRR3722120.349920/146‑1 (MQ=60)
CTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAA > SRR3722120.828473/1‑149 (MQ=60)
GAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCtgtctcttatacacatctccgagcccacgagactaaggcgaatctcgt > SRR3722120.69317/1‑102 (MQ=60)
GCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAAAGGGctgtctcttatacacatctccg > SRR3722120.435100/1‑128 (MQ=60)
|
CATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAAAGGGTATCGATCTGGAAAGCAAAAAA > NZ_CP009273/592459‑592745
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
