Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F5 I97 R1	275	44.0	1733530	96.5%	1672856	122.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	715,111	A→G	I269T (ATC→ACC)	speF ←	ornithine decarboxylase SpeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	715,111	0	A	G	100.0%	181.4 / NA	51	I269T (ATC→ACC)	speF	ornithine decarboxylase SpeF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/28); total (23/28)

TGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTGT  >  NZ_CP009273/714977‑715246

tGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAg                                                                                                                                     >  1:369642/1‑139 (MQ=255)

        cacaGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGCGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCCGCTCACg                                                                                                                               >  1:38342/1‑137 (MQ=255)

            gATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGt                                                                                                                          >  1:612903/1‑138 (MQ=255)

            gATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGt                                                                                                                          >  1:838483/1‑138 (MQ=255)

             aTGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTaa                                                                                                                        >  1:792144/1‑139 (MQ=255)

               gACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACt                                                                                                                      >  1:248554/1‑139 (MQ=255)

                aCCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACt                                                                                                                      <  1:56884/138‑1 (MQ=255)

                aCCAAGTTTATCCACCACTTGGCGGGCGTTAAAAATCGTACCGACGTAGGTGCCTAACTGAATCACAGCGAGGCGAAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGTTAACt                                                                                                                      <  2:38342/138‑1 (MQ=255)

                  cAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACt                                                                                                                      <  2:547285/136‑1 (MQ=255)

                  cAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACt                                                                                                                      >  1:547285/1‑136 (MQ=255)

                          tCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCaaaa                                                                                                            <  2:722391/138‑1 (MQ=255)

                          tCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCaaaa                                                                                                            <  1:808793/138‑1 (MQ=255)

                           cagccacTTGGCGGGCGTTATAAATCGTACCGGCGTGGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACa                                                                                                          <  2:414026/136‑1 (MQ=255)

                                 cTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTgcgc                                                                                                    <  1:165054/139‑1 (MQ=255)

                                    ggcgggcgTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCAt                                                                                                  >  1:558153/1‑138 (MQ=255)

                                    ggcgggcgTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCAt                                                                                                  >  2:12431/1‑138 (MQ=255)

                                      cgggcgTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCaa                                                                                               >  1:216822/1‑139 (MQ=255)

                                         gcgcTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCaactg                                                                                            >  1:414026/1‑136 (MQ=255)

                                         gcgTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGc                                                                                            >  2:635636/1‑139 (MQ=255)

                                          cgTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGcc                                                                                           <  2:838483/139‑1 (MQ=255)

                                                aaaTCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCTGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGAt                                                                                     <  2:350154/139‑1 (MQ=255)

                                                  aTCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATaa                                                                                   <  1:702831/139‑1 (MQ=255)

                                                       aCCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCa                                                                              >  1:141560/1‑139 (MQ=255)

                                                           tcgtAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACg                                                                          >  2:412525/1‑139 (MQ=255)

                                                                    ccTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGt                                                                  <  1:572044/138‑1 (MQ=255)

                                                                           gAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTc                                                          >  1:376094/1‑139 (MQ=255)

                                                                            aaTCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTc                                                          >  2:611462/1‑138 (MQ=255)

                                                                                acaGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGa                                                      <  1:635636/138‑1 (MQ=255)

                                                                                    cGAGGCGGAAAGGACGAGCCTTTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                                 <  2:572478/139‑1 (MQ=255)

                                                                                    cGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                                 <  1:611462/139‑1 (MQ=255)

                                                                                    cGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                                 <  2:361475/139‑1 (MQ=255)

                                                                                    cGAGGCGAAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                                 <  2:453743/139‑1 (MQ=255)

                                                                                               ggACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCAc                                      <  1:233385/139‑1 (MQ=255)

                                                                                                 aCGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCa                                    <  2:792144/139‑1 (MQ=255)

                                                                                                  cGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCAc                                      <  2:89273/136‑1 (MQ=255)

                                                                                                  cGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCAc                                      >  1:89273/1‑136 (MQ=255)

                                                                                                  cGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATACATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAg                                   >  2:81314/1‑139 (MQ=255)

                                                                                                    aGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGcc                                 <  2:603953/139‑1 (MQ=255)

                                                                                                           tttGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGc                           >  2:737804/1‑138 (MQ=255)

                                                                                                            ttGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGc                           <  2:472451/137‑1 (MQ=255)

                                                                                                            ttGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCa                          <  2:369642/138‑1 (MQ=255)

                                                                                                               cacGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGc                      >  2:372091/3‑139 (MQ=255)

                                                                                                                    tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                                 <  1:817293/107‑1 (MQ=255)

                                                                                                                    tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                                 >  2:817293/1‑107 (MQ=255)

                                                                                                                    tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCa                                       <  2:399264/117‑1 (MQ=255)

                                                                                                                    tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCa                                       >  1:399264/1‑117 (MQ=255)

                                                                                                                      cGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCgtg                <  2:248554/138‑1 (MQ=255)

                                                                                                                      cGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCgtg                <  1:110864/138‑1 (MQ=255)

                                                                                                                       ggTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTGCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCgtggt              <  1:63059/139‑1 (MQ=255)

                                                                                                                           cGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTggtggt           <  1:372091/138‑1 (MQ=255)

                                                                                                                                   gcgGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTtgt  >  1:669126/1‑139 (MQ=255)

TGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTGT  >  NZ_CP009273/714977‑715246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCAAACAGGATGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTGTGG  >  NZ_CP009273/714966‑715248

GTCAAACAGGATGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGC                                                                                                                                      >  SRR3722120.375109/1‑151 (MQ=60)

  CAAACAGGATGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCT                                                                                                                                     <  SRR3722120.272883/150‑1 (MQ=60)

        GGATGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGCGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCCGCTCACGC                                                                                                                                >  SRR3722120.38811/1‑149 (MQ=60)

            GTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTA                                                                                                                           >  SRR3722120.849760/1‑150 (MQ=60)

            GTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTA                                                                                                                           >  SRR3722120.621437/1‑150 (MQ=60)

             TAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAAC                                                                                                                         >  SRR3722120.802797/1‑151 (MQ=60)

               GTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTT                                                                                                                       >  SRR3722120.252157/1‑151 (MQ=60)

                  TCACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCT                                                                                                                    >  SRR3722120.554988/1‑151 (MQ=60)

                          GACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAAC                                                                                                             <  SRR3722120.57584/150‑1 (MQ=60)

                                    ATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATC                                                                                                   <  SRR3722120.819692/150‑1 (MQ=60)

                                    ATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATC                                                                                                   >  SRR3722120.565981/1‑150 (MQ=60)

                                      cagCCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAAT                                                                                                >  SRR3722120.219859/4‑151 (MQ=60)

                                         CCACTTGGCGGGCGCTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAActgt                                                                                             >  SRR3722120.420111/1‑147 (MQ=60)

                                           CCTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCAC                                                                                           <  SRR3722120.167316/151‑1 (MQ=60)

                                                       TTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCAT                                                                               >  SRR3722120.143471/1‑151 (MQ=60)

                                                            AATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGG                                                                          <  SRR3722120.712413/151‑1 (MQ=60)

                                                             ATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccg                                                                         >  SRR3722120.45802/1‑96 (MQ=60)

                                                                           GGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCC                                                           >  SRR3722120.381636/1‑151 (MQ=60)

                                                                              GCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAG                                                         <  SRR3722120.580021/150‑1 (MQ=60)

                                                                                          CACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGT                                             <  SRR3722120.644416/150‑1 (MQ=60)

                                                                                              cggagatgtgtataagagacagCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCAC                                        <  SRR3722120.828298/129‑1 (MQ=60)

                                                                                              GCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCAC                                        <  SRR3722120.619938/151‑1 (MQ=60)

                                                                                                  GGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGC                                    >  SRR3722120.90418/1‑151 (MQ=60)

                                                                                                         AGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGC                             <  SRR3722120.236687/151‑1 (MQ=60)

                                                                                                                gggctcggagatgtgtataagagacagTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTC                      <  SRR3722120.35582/124‑1 (MQ=60)

                                                                                                                    CTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCtgtctcttat                   >  SRR3722120.405185/1‑140 (MQ=60)

                                                                                                                                GCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATT       <  SRR3722120.112328/150‑1 (MQ=60)

                                                                                                                                 CGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTGCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTG     <  SRR3722120.63871/151‑1 (MQ=60)

                                                                                                                                   GTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTGTG   >  SRR3722120.678297/1‑151 (MQ=60)

                                                                                                                                     GCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCCCCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCGCCTGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTGTGG  <  SRR3722120.377592/150‑1 (MQ=60)

GTCAAACAGGATGTAGTCACACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTTAGATTTGTGG  >  NZ_CP009273/714966‑715248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`