Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F5 I97 R1	275	44.0	1733530	96.5%	1672856	122.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	923,801	A→G	V284A (GTC→GCC)	cydC ←	cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	923,801	0	A	G	100.0%	165.9 / NA	44	V284A (GTC→GCC)	cydC	cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/25); total (19/25)

TATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGA  >  NZ_CP009273/923667‑923935

tATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCaaa                                                                                                                                    <  1:277172/139‑1 (MQ=255)

   cAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAg                                                                                                                                 >  1:378990/1‑139 (MQ=255)

              aCCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCaaa                                                                                                                                    >  1:100123/1‑125 (MQ=255)

              aCCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCaaa                                                                                                                                    <  2:100123/125‑1 (MQ=255)

                cTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAAcgcg                                                                                                                    <  1:127115/139‑1 (MQ=255)

                cTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAAcgcg                                                                                                                    <  1:841917/139‑1 (MQ=255)

                cTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAAcgcg                                                                                                                    <  2:357043/139‑1 (MQ=255)

                cTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAAcgcg                                                                                                                    <  2:380739/139‑1 (MQ=255)

                        tttGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGtt                                                                                                             <  2:290065/138‑1 (MQ=255)

                                         gagaTACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAAcgc                                                                                           >  1:160233/1‑139 (MQ=255)

                                                gtacgGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGa                                                                                    >  1:653700/1‑139 (MQ=255)

                                                    ggCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGcc                                                                                <  2:493106/139‑1 (MQ=255)

                                                    ggCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGc                                                                                 >  1:59196/1‑138 (MQ=255)

                                                         agGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAAcgcg                                                                                                                    >  1:618464/1‑98 (MQ=255)

                                                         agGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAAcgcg                                                                                                                    <  2:618464/98‑1 (MQ=255)

                                                         agGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCca                                                                           >  1:626151/1‑139 (MQ=255)

                                                         agGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCca                                                                           <  2:609104/139‑1 (MQ=255)

                                                                     gCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCa                                                                               >  1:292821/1‑123 (MQ=255)

                                                                     gCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCa                                                                               <  2:292821/123‑1 (MQ=255)

                                                                     gCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAgg                                                                 <  1:344398/137‑1 (MQ=255)

                                                                     gCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                               >  2:42489/1‑139 (MQ=255)

                                                                      ccccAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                               <  2:331147/138‑1 (MQ=255)

                                                                                tGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAAcg                                                    >  2:231792/1‑139 (MQ=255)

                                                                                  aaaTGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACg                                                                                  <  1:606455/107‑1 (MQ=255)

                                                                                  aaaTGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACg                                                                                  >  2:606455/1‑107 (MQ=255)

                                                                                  aaaTGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAAcgcg                                                  <  2:59196/139‑1 (MQ=255)

                                                                                  aaaTGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAAcg                                                    <  2:153401/137‑1 (MQ=255)

                                                                                         cccGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGc                                                                       <  1:637663/111‑1 (MQ=255)

                                                                                         cccGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGc                                                                       >  2:637663/1‑111 (MQ=255)

                                                                                          ccGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCATACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGag                                          <  2:240488/139‑1 (MQ=255)

                                                                                          ccGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGag                                          <  2:653700/139‑1 (MQ=255)

                                                                                          ccGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGag                                          <  2:378990/139‑1 (MQ=255)

                                                                                          ccGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGag                                          <  2:589178/139‑1 (MQ=255)

                                                                                            gTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                               >  1:824416/1‑116 (MQ=255)

                                                                                            gTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                               <  2:824416/116‑1 (MQ=255)

                                                                                                 tGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCAt                                    >  2:827529/1‑138 (MQ=255)

                                                                                                   gTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTa                                  <  2:626151/138‑1 (MQ=255)

                                                                                                    tGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATc                                >  2:29058/1‑139 (MQ=255)

                                                                                                          tGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGc                          >  1:684368/1‑139 (MQ=255)

                                                                                                              tCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGAc                       >  1:819625/1‑138 (MQ=255)

                                                                                                                      ggCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCa                                                                                                        >  2:503525/1‑49 (MQ=255)

                                                                                                                      ggCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCa                                                                                                        <  1:503525/49‑1 (MQ=255)

                                                                                                                           aCGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGtt          <  1:295664/138‑1 (MQ=255)

                                                                                                                                cAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGAtt    >  2:551156/1‑139 (MQ=255)

                                                                                                                                  gaaggCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGa  <  1:231792/139‑1 (MQ=255)

TATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGA  >  NZ_CP009273/923667‑923935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGC  >  NZ_CP009273/923659‑923946

AGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGG                                                                                                                                           >  SRR3722120.384575/1‑151 (MQ=60)

       GTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTA                                                                                                                                    <  SRR3722120.281167/151‑1 (MQ=60)

           CAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTActgt                                                                                                                                >  SRR3722120.101462/1‑147 (MQ=60)

                       CCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGC                                                                                                                    <  SRR3722120.128834/151‑1 (MQ=60)

                       CCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGC                                                                                                                    <  SRR3722120.853242/151‑1 (MQ=60)

                                    cgatgtttttttacaagcagaaggcggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAAC                                                                                                        <  SRR3722120.510727/71‑1 (MQ=60)

                                      CCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCC                                                                                                     >  SRR3722120.162425/1‑151 (MQ=60)

                                             GTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGAC                                                                                              >  SRR3722120.662696/1‑151 (MQ=60)

                                                 GAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCC                                                                                           >  SRR3722120.59922/1‑150 (MQ=60)

                                                      ACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCctgtctcttatacacatctccgagcccacg                                                                                      >  SRR3722120.627067/1‑120 (MQ=60)

                                                      ACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCAC                                                                                     >  SRR3722120.634836/1‑151 (MQ=60)

                                                         cggagatgtgtataagagacagCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGC                                                                                  <  SRR3722120.614874/129‑1 (MQ=60)

                                                                  GGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCctgtct                                                                         >  SRR3722120.297014/1‑145 (MQ=60)

                                                                    gatgtgtataagagacagGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCAC                                                                       <  SRR3722120.646459/133‑1 (MQ=60)

                                                                            TGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAA                                                                 <  SRR3722120.349380/149‑1 (MQ=60)

                                                                                         GAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCtgtctcttatac                                                  >  SRR3722120.835499/1‑139 (MQ=60)

                                                                                                       ACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCG                                    >  SRR3722120.693744/1‑151 (MQ=60)

                                                                                                           GTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACA                                 >  SRR3722120.830652/1‑150 (MQ=60)

                                                                                                                                  AACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGG          <  SRR3722120.299888/150‑1 (MQ=60)

                                                                                                                                         AGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGC  <  SRR3722120.235063/151‑1 (MQ=60)

AGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGTCAGTTCAGATTGACGGCGTTGCGC  >  NZ_CP009273/923659‑923946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`