Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F5 I97 R1 275 44.0 1733530 96.5% 1672856 122.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACAACAGCGTGGTACGCAGAATACGATGAATGGGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAAT  >  NZ_CP009273/728523‑728814
                                                                                                                                                   |                                                                                                                                                
GGACAACAGCGTGGTACGCAGAATACGATGAATGGGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCC                                                                                                                                               >  SRR3722120.628288/1‑151 (MQ=42)
     ACAGCGTGGTACGCAGAATACGATGAATGGGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGC                                                                                                                                          <  SRR3722120.171857/151‑1 (MQ=34)
           aagagacagGAATACGATGAATGGGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGG                                                                                                                                     <  SRR3722120.520298/141‑1 (MQ=6)
                 GCAGAATACGATGAATGGGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATA                                                                                                                              <  SRR3722120.558598/151‑1 (MQ=21)
                      ATACGATGAATGGGGTAAGCTGAGGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCtgtctcttat                                                                                                                          >  SRR3722120.313222/1‑140 (MQ=5)
                                  GGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATT                                                                                                             >  SRR3722120.494429/1‑151 (MQ=19)
                                           GCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAA                                                                                                     >  SRR3722120.501721/1‑150 (MQ=8)
                                            gtataagagacagACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAA                                                                                                    <  SRR3722120.317169/137‑1 (MQ=10)
                                                            CGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTA                                                                                   <  SRR3722120.423630/151‑1 (MQ=21)
                                                                       CAGCAGTTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGACAGGGGGATGGAATTTTTATCAGTATCCGT                                                                        >  SRR3722120.540981/1‑151 (MQ=20)
                                                                                          acgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATAT                                                     <  SRR3722120.831345/103‑1 (MQ=60)
                                                                                           cgtgggctcggagatgtgtataagagacagGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATAT                                                     <  SRR3722120.484147/120‑1 (MQ=52)
                                                                                            agagacagGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAG                                                   <  SRR3722120.618696/143‑1 (MQ=53)
                                                                                            GGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATAT                                                     >  SRR3722120.122151/1‑149 (MQ=46)
                                                                                                      ATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGG                                         >  SRR3722120.387545/1‑151 (MQ=60)
                                                                                                                    GGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAA                           <  SRR3722120.594128/151‑1 (MQ=60)
                                                                                                                         GTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGC                      >  SRR3722120.104114/1‑151 (MQ=60)
                                                                                                                                   CGCCACCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTctgtctcttatacacatctccgagcc            >  SRR3722120.597333/1‑125 (MQ=60)
                                                                                                                                        CCGCTATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCAT       <  SRR3722120.537833/151‑1 (MQ=60)
                                                                                                                                             ATTATGCCCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAAT  <  SRR3722120.496537/151‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                
GGACAACAGCGTGGTACGCAGAATACGATGAATGGGGTAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGGCTGAAAGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAAT  >  NZ_CP009273/728523‑728814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑