Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I179 R1
|
182 |
40.3 |
1547118 |
96.8% |
1497610 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
467,406 |
Δ1 bp |
coding (1315/1782 nt) |
mdlB → |
SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 467,406 | 0 | C | . | 100.0%
| 115.4
/ NA
| 27 | coding (1315/1782 nt) | mdlB | SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (11/16); total (11/16) |
ACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGC > NZ_CP009273/467271‑467538
|
aCGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGgggggga > 2:565228/1‑138 (MQ=255)
aCGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGgggggga < 2:221521/139‑2 (MQ=255)
gtcgtcCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAg < 1:718727/139‑1 (MQ=255)
ttAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGa < 1:228061/139‑1 (MQ=255)
cGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTg > 2:193064/1‑139 (MQ=255)
caGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCgg < 2:319415/139‑1 (MQ=255)
tGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGc > 2:722091/1‑139 (MQ=255)
ggCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAg < 1:346890/137‑1 (MQ=255)
ggCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAg > 2:346890/1‑137 (MQ=255)
gCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGa < 1:105988/139‑1 (MQ=255)
cAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGAc < 1:656447/139‑1 (MQ=255)
cAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGAc < 2:269759/139‑1 (MQ=255)
gTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTAt > 1:469902/1‑139 (MQ=255)
agcaAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTa > 2:574947/1‑138 (MQ=255)
agcaAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGGATTTa > 2:760148/1‑138 (MQ=255)
gcaAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTaca < 1:148187/139‑1 (MQ=255)
aTCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACAcgccg < 1:722091/139‑1 (MQ=255)
tCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCt > 2:202558/1‑104 (MQ=255)
tCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCt < 1:202558/104‑1 (MQ=255)
tgCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAg < 1:574947/139‑1 (MQ=255)
tgCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAg < 2:745877/139‑1 (MQ=255)
tCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAAtctc > 1:234285/1‑139 (MQ=255)
cTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTac > 2:251509/1‑107 (MQ=255)
cTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTac < 1:251509/107‑1 (MQ=255)
cTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAAtctct > 2:192269/1‑138 (MQ=255)
cTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAAtctct < 2:234285/138‑1 (MQ=255)
gCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGt < 1:749585/139‑1 (MQ=255)
cGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTggg > 1:453367/1‑138 (MQ=255)
gCTGGGG‑GGGATATCTCCGAAGAACGCGTCGGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGc < 2:480972/139‑1 (MQ=255)
|
ACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGC > NZ_CP009273/467271‑467538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGG > NZ_CP009273/467256‑467553
|
GGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGG > SRR3722133.273432/1‑151 (MQ=60)
CTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGGGG > SRR3722133.659842/1‑151 (MQ=60)
ATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGAT < SRR3722133.12076/151‑1 (MQ=60)
GGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAAC < SRR3722133.728048/151‑1 (MQ=60)
ATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAAC < SRR3722133.231170/151‑1 (MQ=60)
gctcggagatgtgtataagagacaGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCA < SRR3722133.205256/127‑1 (MQ=60)
GCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATT > SRR3722133.476286/1‑151 (MQ=60)
CGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT < SRR3722133.351760/151‑1 (MQ=60)
GGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACA < SRR3722133.107370/151‑1 (MQ=60)
GCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACAC < SRR3722133.664992/151‑1 (MQ=60)
cggagatgtgtataagagacaGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGG < SRR3722133.254936/130‑1 (MQ=60)
AGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGC < SRR3722133.150134/151‑1 (MQ=60)
GATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCA < SRR3722133.731448/151‑1 (MQ=60)
GTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAA < SRR3722133.582588/151‑1 (MQ=60)
GGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCT > SRR3722133.237476/1‑151 (MQ=60)
TTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGC > SRR3722133.459530/1‑150 (MQ=60)
CGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGC < SRR3722133.759305/151‑1 (MQ=60)
ACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACctgtctcttatacacatctccgagcccacgagaccgtactagatctcgt > SRR3722133.660380/1‑102 (MQ=60)
GGGGGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAActgtctcttatacacatctccgagcccacgacacc > SRR3722133.152968/1‑116 (MQ=60)
|
GGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGG > NZ_CP009273/467256‑467553
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
