Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F5 I176 R1 180 42.9 1710034 96.5% 1650182 119.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGC  >  NZ_CP009273/755699‑755985
                                                                                                                                             |                                                                                                                                                 
acgagattcgccttagtctcgtgggctcggagatgtgtataagagacagCTCTTTCACCTGGTCGCCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCAC                                                                                                                                          <  SRR3722130.258142/102‑1 (MQ=60)
          cggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGCCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAA                                                                                                                                <  SRR3722130.493132/97‑1 (MQ=60)
                                           agcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATT                                                                                               <  SRR3722130.403287/87‑1 (MQ=60)
                                                ACTCTTTCACCTGGTCGCCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGG                                                                                          <  SRR3722130.98884/151‑1 (MQ=60)
                                                   CTTTCACCTGGTCGCCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCG                                                                                        >  SRR3722130.356601/1‑150 (MQ=60)
                                                            gtctcgtgggctcggagatgtgtataagagacagGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGC                                                                              <  SRR3722130.497030/117‑1 (MQ=60)
                                                                 CCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGG                                                                         >  SRR3722130.626191/1‑151 (MQ=60)
                                                                 CCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGG                                                                         >  SRR3722130.563480/1‑151 (MQ=60)
                                                                      CCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCtgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtctt                                                                    >  SRR3722130.752783/1‑93 (MQ=60)
                                                                                      GACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTctgtctcttatacacatctccgagcccacgagactaaggcg                                                     >  SRR3722130.272206/1‑109 (MQ=60)
                                                                                        ggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGAC                                                  <  SRR3722130.286288/98‑1 (MQ=60)
                                                                                        CGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGAC                                                  <  SRR3722130.491854/151‑1 (MQ=60)
                                                                                                  aagagacagGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTG                                        <  SRR3722130.827973/142‑1 (MQ=60)
                                                                                                    GAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCtgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatg                                      >  SRR3722130.511724/1‑100 (MQ=60)
                                                                                                        AAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAA                                  <  SRR3722130.314882/151‑1 (MQ=60)
                                                                                                          AGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAAC                                <  SRR3722130.579663/151‑1 (MQ=60)
                                                                                                          AGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACTCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAAC                                <  SRR3722130.729584/151‑1 (MQ=60)
                                                                                                                                        GAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGC  <  SRR3722130.549208/151‑1 (MQ=60)
                                                                                                                                             |                                                                                                                                                 
TGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTCAACCACGAATGGGACGAAGAGTACCCGAACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGC  >  NZ_CP009273/755699‑755985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑