Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I96 R1
|
227 |
23.3 |
882352 |
98.0% |
864704 |
126.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
757,924 |
T→C |
L316P (CTG→CCG) |
odhB → |
2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 757,924 | 0 | T | C | 100.0%
| 69.8
/ NA
| 21 | L316P (CTG→CCG) | odhB | 2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/10); total (11/10) |
TGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTC > NZ_CP009273/757789‑758058
|
tGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGAc < 1:224696/139‑1 (MQ=255)
cgccgcGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAAc < 2:241093/137‑1 (MQ=255)
cgcgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtcac > 2:133245/1‑139 (MQ=255)
cgcgGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTtcacca > 1:59807/1‑139 (MQ=255)
ccGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGgtggtg > 2:275520/1‑139 (MQ=255)
ccGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGgtggtg > 2:237749/1‑139 (MQ=255)
ccGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGgtggtg > 2:146602/1‑139 (MQ=255)
ttCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGtt < 1:3575/139‑1 (MQ=255)
cGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGtt > 1:178215/1‑139 (MQ=255)
tGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGtt > 1:227658/1‑132 (MQ=255)
tGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGtt < 2:227658/132‑1 (MQ=255)
aTGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt < 2:59807/139‑1 (MQ=255)
aTGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt < 1:338693/139‑1 (MQ=255)
aTGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCt < 1:149509/139‑1 (MQ=255)
cGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGcc > 1:405753/1‑138 (MQ=255)
aTCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACccgc > 2:27118/1‑139 (MQ=255)
cTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGt > 2:120187/1‑87 (MQ=255)
cTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGt < 1:120187/87‑1 (MQ=255)
gTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCa > 2:52558/1‑135 (MQ=255)
aaGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGa < 1:237749/139‑1 (MQ=255)
gATCCGACCGGTGGTATCTTCACCCTCACCAACGGTGGGGTGTTCGGTTCCCTGATGTCTACGCGGATCAACAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTc < 1:133245/139‑1 (MQ=255)
|
TGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTC > NZ_CP009273/757789‑758058
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTCAGGTTGAGATC > NZ_CP009273/757788‑758069
|
ATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACT < SRR3722119.227085/151‑1 (MQ=60)
TTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCAT > SRR3722119.60363/1‑151 (MQ=60)
CGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTC > SRR3722119.180058/1‑151 (MQ=60)
CCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCC > SRR3722119.230067/1‑151 (MQ=60)
GTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGA < SRR3722119.3606/151‑1 (MQ=60)
cgccttagtctcgtgggctcggagatgtgtataagagacagAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTC < SRR3722119.121387/109‑1 (MQ=60)
GATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGC < SRR3722119.151049/151‑1 (MQ=60)
GATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGC < SRR3722119.342155/151‑1 (MQ=60)
GTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCG > SRR3722119.409812/1‑150 (MQ=60)
CAAGCTGACCGTTGAAGATCCGACCGGTGGTAACTTCACCATCCCCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAAT < SRR3722119.240235/151‑1 (MQ=60)
AGATCCGACCGGTGGTATCTTCACCCTCACCAACGGTGGGGTGTTCGGTTCCCTGATGTCTACGCGGATCAACAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTCAGGTTGAGATC < SRR3722119.134618/151‑1 (MQ=60)
|
ATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAGACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGACGGCAAGCTGACCGTTGAAGATCTGACCGGTGGTAACTTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGGTATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTCAGGTTGAGATC > NZ_CP009273/757788‑758069
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
