Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F5 I102 R1
|
278 |
39.1 |
2251254 |
95.8% |
2156701 |
87.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
803,404 |
A→C |
intergenic (‑39/+20) |
ybhB ← / ← bioA |
kinase inhibitor/adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 803,404 | 0 | A | C | 100.0%
| 145.1
/ NA
| 40 | intergenic (‑39/+20) | ybhB/bioA | kinase inhibitor/adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/24); total (16/24) |
GATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCA > NZ_CP009273/803317‑803485
|
gATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAcccc < 1:88705/91‑1 (MQ=255)
gATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAcccc < 1:756959/91‑1 (MQ=255)
aTGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCt > 2:679145/1‑91 (MQ=255)
tttATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGAc < 2:1122750/91‑1 (MQ=255)
tCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTtct > 1:85992/1‑91 (MQ=255)
aTCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTa > 2:233004/1‑91 (MQ=255)
tCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTAt > 1:245333/1‑91 (MQ=255)
ttACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAcccc < 2:838720/59‑1 (MQ=255)
ttACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAcccc > 1:838720/1‑59 (MQ=255)
tACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATg > 2:841150/1‑91 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:1028137/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 2:85992/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 2:432891/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 2:243327/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:695694/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:679145/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:635365/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:507455/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:488655/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:416922/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:1050791/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:255966/91‑1 (MQ=255)
tgatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:1102460/91‑1 (MQ=255)
gatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt < 1:407778/90‑1 (MQ=255)
gatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGttt > 2:407778/1‑90 (MQ=255)
gatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTc > 1:604891/1‑91 (MQ=255)
gatgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTc > 1:197513/1‑91 (MQ=255)
tgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCAt > 1:676589/1‑91 (MQ=255)
tgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCAt > 1:307414/1‑91 (MQ=255)
gTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCt > 2:8393/1‑91 (MQ=255)
aCCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgcgc < 2:245333/91‑1 (MQ=255)
ccccccTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTGACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgcgtg > 2:177252/1‑89 (MQ=255)
cTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGc < 1:1094539/91‑1 (MQ=255)
ttGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAGAATGTTTCATCCTGTACCGCGCGGTTAACCGCt > 1:1047271/1‑91 (MQ=255)
ttGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCt < 1:1088925/91‑1 (MQ=255)
gTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc < 1:637125/91‑1 (MQ=255)
gTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc > 2:637125/1‑91 (MQ=255)
gTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGc < 1:841150/91‑1 (MQ=255)
ttGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCgg < 2:604891/91‑1 (MQ=255)
gCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTc > 2:531414/1‑91 (MQ=255)
aaaGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGAc < 2:1022161/91‑1 (MQ=255)
aaaGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGAc < 1:1005300/91‑1 (MQ=255)
aaGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACg < 2:881657/91‑1 (MQ=255)
tAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCa > 2:549708/1‑91 (MQ=255)
|
GATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCA > NZ_CP009273/803317‑803485
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTG > NZ_CP009273/803304‑803492
|
TTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAC < SRR3722125.371666/101‑1 (MQ=60)
TTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAC < SRR3722125.621927/101‑1 (MQ=60)
TTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAC < SRR3722125.120725/101‑1 (MQ=60)
GATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGAC < SRR3722125.90361/101‑1 (MQ=60)
GATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGAC < SRR3722125.773343/101‑1 (MQ=60)
CGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCT > SRR3722125.87593/1‑101 (MQ=60)
TTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTAT > SRR3722125.250262/1‑101 (MQ=60)
GCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACctgtctcttatacacatctccg > SRR3722125.857185/1‑79 (MQ=60)
GATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTC > SRR3722125.201408/1‑101 (MQ=60)
GATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTC > SRR3722125.617923/1‑101 (MQ=60)
TCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCAT > SRR3722125.691169/1‑101 (MQ=60)
TCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCAT > SRR3722125.313719/1‑101 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.649059/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.416371/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.693796/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.518221/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.710692/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.498976/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.425735/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.313900/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.261128/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.1051178/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.1127298/101‑1 (MQ=60)
TGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTAC < SRR3722125.1074391/101‑1 (MQ=60)
CCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAGAATGTTTCATCCTGTACCGCGCGGTTAACCGCT > SRR3722125.1070784/1‑101 (MQ=60)
CTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGA < SRR3722125.1119176/101‑1 (MQ=60)
TTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGAC < SRR3722125.1113433/101‑1 (MQ=60)
GTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGC < SRR3722125.650863/101‑1 (MQ=60)
GTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGC < SRR3722125.859669/101‑1 (MQ=60)
AAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTG < SRR3722125.1027781/101‑1 (MQ=60)
GTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCctgtctcttatacacatctccgagcccacgagactaggcatgat > SRR3722125.333426/1‑57 (MQ=60)
GTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACctgtctcttatac > SRR3722125.475706/1‑88 (MQ=60)
GTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTG > SRR3722125.1044388/1‑101 (MQ=60)
GTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTG > SRR3722125.243639/1‑101 (MQ=60)
GTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTG > SRR3722125.468514/1‑101 (MQ=60)
|
TTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTG > NZ_CP009273/803304‑803492
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
